Adverse Effects of Methylglyoxal on Transcriptome and Metabolic Changes in Visceral Adipose Tissue in a Prediabetic Rat Model

Excessive methylglyoxal (MG) production contributes to metabolic and vascular changes by increasing inflammatory processes, disturbing regulatory mechanisms and exacerbating tissue dysfunction. MG accumulation in adipocytes leads to structural and functional changes. We used transcriptome analysis to investigate the effect of MG on metabolic changes in the visceral adipose tissue of hereditary hypetriglyceridaemic rats, a non-obese model of metabolic syndrome. Compared to controls, 4-week intragastric MG administration impaired glucose tolerance (p < 0.05) and increased glycaemia (p < 0.01) and serum levels of MCP-1 and TNFα (p < 0.05), but had no effect on serum adiponectin or leptin. Adipose tissue insulin sensitivity and lipolysis were impaired (p < 0.05) in MG-treated rats. In addition, MG reduced the expression of transcription factor Nrf2 (p < 0.01), which controls antioxidant and lipogenic genes. Increased expression of Mcp-1 and TNFα (p < 0.05) together with activation of the SAPK/JNK signaling pathway can promote chronic inflammation in adipose tissue. Transcriptome network analysis revealed the over-representation of genes involved in insulin signaling (Irs1, Igf2, Ide), lipid metabolism (Nr1d1, Lpin1, Lrpap1) and angiogenesis (Dusp10, Tp53inp1).

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Systems-wide effects of short-term feed deprivation in obese mice

Scientific Reports ◽

10.1038/s41598-021-85020-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Daniel Andersen ◽

Henrik Munch Roager ◽

Li Zhang ◽

Janne Marie Moll ◽

Henrik Lauritz Frandsen ◽

...

Keyword(s):

Adipose Tissue ◽

Butyric Acid ◽

Visceral Adipose Tissue ◽

Animal Studies ◽

Uncoupling Protein ◽

Metabolic Changes ◽

Obese Mice ◽

Short Term ◽

Feed Deprivation ◽

Visceral Adipose

AbstractWhile prolonged fasting induces significant metabolic changes in humans and mice, less is known about systems-wide metabolic changes in response to short-term feed deprivation, which is used in experimental animal studies prior to metabolic challenge tests. We here performed a systems biology-based investigation of connections between gut bacterial composition and function, inflammatory and metabolic parameters in the intestine, liver, visceral adipose tissue, blood and urine in high-fat fed, obese mice that were feed deprived up to 12 h. The systems-wide analysis revealed that feed deprivation linked to enhanced intestinal butyric acid production and expression of the gene encoding the pro-thermogenic uncoupling protein UCP1 in visceral adipose tissue of obese mice. Ucp1 expression was also positively associated with Il33 expression in ileum, colon and adipose tissue as well as with the abundance of colonic Porphyromonadaceae, the latter also correlating to cecal butyric acid levels. Collectively, the data highlighted presence of a multi-tiered system of inter-tissue communication involving intestinal, immune and metabolic functions which is affected by feed deprivation in obese mice, thus pointing to careful use of short-feed deprivation in metabolic studies using obese mice.

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Progranulin serum levels and gene expression in subcutaneous vs visceral adipose tissue of severely obese patients undergoing bariatric surgery

Clinical Endocrinology ◽

10.1111/cen.14040 ◽

2019 ◽

Vol 91 (3) ◽

pp. 400-410 ◽

Cited By ~ 3

Author(s):

Judith Brock ◽

Andreas Schmid ◽

Thomas Karrasch ◽

Petra Pfefferle ◽

Jutta Schlegel ◽

...

Keyword(s):

Gene Expression ◽

Bariatric Surgery ◽

Adipose Tissue ◽

Visceral Adipose Tissue ◽

Serum Levels ◽

Obese Patients ◽

Severely Obese ◽

Visceral Adipose

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Systems-wide effects of short-term feed deprivation in obese mice

10.21203/rs.3.rs-44356/v1 ◽

2020 ◽

Author(s):

Daniel Andersen ◽

Henrik Munch Roager ◽

Li Zhang ◽

Janne Marie Moll ◽

Henrik Lauritz Frandsen ◽

...

Keyword(s):

Adipose Tissue ◽

Butyric Acid ◽

Visceral Adipose Tissue ◽

Animal Studies ◽

Uncoupling Protein ◽

Metabolic Changes ◽

Obese Mice ◽

Short Term ◽

Feed Deprivation ◽

Visceral Adipose

Abstract Background While prolonged fasting induces significant metabolic changes in humans and mice, less is known about systems-wide metabolic changes in response to short-term feed deprivation, which traditionally has been used in experimental animal studies prior to metabolic challenge tests. Methods We here performed a systems biology-based investigation of connections between gut bacterial composition and function, inflammatory and metabolic parameters in the intestine, liver, visceral adipose tissue, blood and urine in obese mice that were feed deprived for varying durations up to 12 hours. Results Our analysis revealed that increased duration of feed deprivation linked to enhanced intestinal butyric acid production and expression of the gene encoding the pro-thermogenic uncoupling protein UCP1 in visceral adipose tissue of obese mice. Ucp1 expression was also positively associated with Il33 expression in ileum, colon and adipose tissue as well as with the abundance of colonic Porphyromonadaceae, the latter also correlating to cecal butyric acid levels. Conclusions The data highlighted presence of a three-tiered system of inter-tissue communication involving intestinal, immune and metabolic functions which is affected by the duration of feed deprivation in obese mice.

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Systems-wide Effects of Short-term Feed Deprivation in Obese Mice

10.21203/rs.3.rs-109202/v1 ◽

2020 ◽

Author(s):

Daniel Andersen ◽

Henrik Roager ◽

Li Zhang ◽

Janne Moll ◽

Henrik Frandsen ◽

...

Keyword(s):

Adipose Tissue ◽

Butyric Acid ◽

Visceral Adipose Tissue ◽

Animal Studies ◽

Uncoupling Protein ◽

Metabolic Changes ◽

Obese Mice ◽

Short Term ◽

Feed Deprivation ◽

Visceral Adipose

Abstract While prolonged fasting induces significant metabolic changes in humans and mice, less is known about systems-wide metabolic changes in response to short-term feed deprivation, which is used in experimental animal studies prior to metabolic challenge tests, and hence, information on the effects of varying length of feed deprivation is warranted. We here performed a systems biology-based investigation of connections between gut bacterial composition and function, inflammatory and metabolic parameters in the intestine, liver, visceral adipose tissue, blood and urine in obese mice that were feed deprived for varying durations up to 12 hours. The systems-wide analysis revealed that increased duration of feed deprivation linked to enhanced intestinal butyric acid production and expression of the gene encoding the pro-thermogenic uncoupling protein UCP1 in visceral adipose tissue of obese mice. Ucp1 expression was also positively associated with Il33 expression in ileum, colon and adipose tissue as well as with the abundance of colonic Porphyromonadaceae, the latter also correlating to cecal butyric acid levels. Collectively, the data highlighted presence of a three-tiered system of inter-tissue communication involving intestinal, immune and metabolic functions which is affected by the duration of feed deprivation in obese mice, thus pointing to careful use of short-feed deprivation in metabolic studies using mice.

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17β-Estradiol suppresses visceral adipogenesis and activates brown adipose tissue-specific gene expression

Hormone Molecular Biology and Clinical Investigation ◽

10.1515/hmbci-2016-0031 ◽

2016 ◽

Vol 0 (0) ◽

Cited By ~ 3

Author(s):

Saad Misfer Al-Qahtani ◽

Galyna Bryzgalova ◽

Ismael Valladolid-Acebes ◽

Marion Korach-André ◽

Karin Dahlman-Wright ◽

...

Keyword(s):

Adipose Tissue ◽

Visceral Adipose Tissue ◽

Specific Gene ◽

Lipogenic Genes ◽

Brown Adipose ◽

17Β Estradiol ◽

Magnetic Resonance Imaging Mri ◽

Visceral Adipose ◽

Targeting Strategy

AbstractBoth functional ovaries and estrogen replacement therapy (ERT) reduce the risk of type 2 diabetes (T2D). Understanding the mechanisms underlying the antidiabetic effects of 17β-estradiol (E2) may permit the development of a molecular targeting strategy for the treatment of metabolic disease. This study examines how the promotion of insulin sensitivity and weight loss by E2 treatment in high-fat-diet (HFD)-fed mice involve several anti-adipogenic processes in the visceral adipose tissue. Magnetic resonance imaging (MRI) revealed specific reductions in visceral adipose tissue volume in HFD+E2 mice, compared with HFD mice. This loss of adiposity was associated with diminished visceral adipocyte size and reductions in expression of lipogenic genes, adipokines and of the nuclear receptor

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Systems-wide effects of short-term feed deprivation in obese mice

10.21203/rs.3.rs-44356/v2 ◽

2020 ◽

Author(s):

Daniel Andersen ◽

Henrik Munch Roager ◽

Li Zhang ◽

Janne Marie Moll ◽

Henrik Lauritz Frandsen ◽

...

Keyword(s):

Adipose Tissue ◽

Butyric Acid ◽

Visceral Adipose Tissue ◽

Animal Studies ◽

Uncoupling Protein ◽

Metabolic Changes ◽

Obese Mice ◽

Short Term ◽

Feed Deprivation ◽

Visceral Adipose

Abstract Background: While prolonged fasting induces significant metabolic changes in humans and mice, less is known about systems-wide metabolic changes in response to short-term feed deprivation, which traditionally has been used in experimental animal studies prior to metabolic challenge tests. Methods: We here performed a systems biology-based investigation of connections between gut bacterial composition and function, inflammatory and metabolic parameters in the intestine, liver, visceral adipose tissue, blood and urine in obese mice that were feed deprived for varying durations up to 12 hours. Results: Our analysis revealed that increased duration of feed deprivation linked to enhanced intestinal butyric acid production and expression of the gene encoding the pro-thermogenic uncoupling protein UCP1 in visceral adipose tissue of obese mice. Ucp1 expression was also positively associated with Il33 expression in ileum, colon and adipose tissue as well as with the abundance of colonic Porphyromonadaceae, the latter also correlating to cecal butyric acid levels. Conclusions: The data highlighted presence of a three-tiered system of inter-tissue communication involving intestinal, immune and metabolic functions which is affected by the duration of feed deprivation in obese mice.

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Uroven' visfatina syvorotki krovi i ekspressiyagena visfatina (PBEF1) v podkozhnoyi vistseral'noy zhirovoy tkani u detey

Obesity and metabolism ◽

10.14341/2071-8713-5083 ◽

2010 ◽

Vol 7 (4) ◽

pp. 20-23

Author(s):

A V Kosygina ◽

V V Sosunov ◽

V A Peterkova ◽

I I Dedov

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Visceral Adipose Tissue ◽

Mrna Level ◽

Serum Levels ◽

Normal Weight ◽

Anthropometric Parameters ◽

Overweight Group ◽

Normal Body Weight ◽

Visceral Adipose

The aim of this study was to investigate the characteristics of visfatin - PBEF1 - gene expression in subcutaneous and visceral adipose tissue and serum levels of visfatin in children with relation to age and anthropometric parameters. The study included 52 patients (27 boys (52%), 25 girls (48%) aged from 2,5 to 18 years (13,8 [7,4-15,1] years)), who underwent an elective surgical intervention. PBEF1 mRNA level was measured by real-time PCR and serum level of visfatin was quantified by immunoenzyme assay. According to our study visfatin serum concentration in children with normal body weight was 9,2 [7,4-11,6] ng/ml, whereas in the overweight group - 9,5 [7,5-11,1] ng/ml (p=0,5). No statistically significant gender difference in serum visfatin levels was observed. No correlation between visfatin levels and age, pubertal stages and anthropometric indices in children was found. Statistically significant differences in the level of gene expression between subcutaneous and visceral adipose tissue were found (р=0,002) in the total group of children surveyed and children with normal weight, while there were no depot-specific differences in overweight children. The study did not reveal any dependence of PBEF1 expression on age and sex of children. Expression of PBEF1 in adipose tissue decreases with puberty (PBEF1VATγ=-0,24, р=0,02; PBEF1SATγ=-0,25, р=0,02). PBEF1 expression in adipose tissue was not correlated with the serum visfatin (R=-0,06, р=0,6).

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Innate Immune System Orchestrates Metabolic Homeostasis and Dysfunction in Visceral Adipose Tissue During Obesity

Frontiers in Immunology ◽

10.3389/fimmu.2021.702835 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yu Zhen ◽

Wentao Shu ◽

Xintong Hou ◽

Yinan Wang

Keyword(s):

Adipose Tissue ◽

Immune System ◽

Chronic Inflammation ◽

Visceral Adipose Tissue ◽

Immune Cells ◽

Innate Immune ◽

Health Concern ◽

Innate Immune Cells ◽

Functional Changes ◽

Visceral Adipose

Arising incidence of metabolic disorders and related diseases caused by obesity is a global health concern. Elucidating the role of the immune system in this process will help to understand the related mechanisms and develop treatment strategies. Here, we have focused on innate immune cells in visceral adipose tissue (VAT) and summarized the roles of these cells in maintaining the homeostasis of VAT. Furthermore, this review reveals the importance of quantitative and functional changes of innate immune cells when the metabolic microenvironment changes due to obesity or excess lipids, and confirms that these changes eventually lead to the occurrence of chronic inflammation and metabolic diseases of VAT. Two perspectives are reviewed, which include sequential changes in various innate immune cells in the steady state of VAT and its imbalance during obesity. Cross-sectional interactions between various innate immune cells at the same time point are also reviewed. Through delineation of a comprehensive perspective of VAT homeostasis in obesity-induced chronic inflammation, and ultimately metabolic dysfunction and disease, we expect to clarify the complex interactive networks among distinct cell populations and propose that these interactions should be taken into account in the development of biotherapeutic strategies.

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