scholarly journals Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta

2020 ◽  
Vol 10 (21) ◽  
pp. 7835
Author(s):  
Uros Josic ◽  
Tatjana Maravic ◽  
Maurizio Bossù ◽  
Milena Cadenaro ◽  
Allegra Comba ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Osteogenesis Imperfecta ◽  
Ultrastructural Level ◽  
Morphological Characterization ◽  
Deciduous Teeth ◽  
Tem Analysis ◽  
Correlative Analysis ◽  
Transmission Electron ◽  
Dentin Collagen

The purpose of this study was to clarify the structural and ultrastructural alterations of the enamel and dentin collagen network in the deciduous teeth of children affected by osteogenesis imperfecta (OI) using field-emission in-lens scanning electron microscopy (FEI-SEM) and transmission electron microscopy (TEM) analyses. Exfoliated primary teeth were collected from children with a diagnosis of OI and from healthy individuals (N = 24). Tooth slices containing both dentin and enamel were fixed, dehydrated and dried, gold sputtered, and observed using FEI-SEM. Additional dentin fragments were decalcified, dehydrated, embedded in resin, cut, and processed for TEM analysis. Under FEI-SEM, the enamel in OI-affected children showed an irregular prism distribution with the enamel hydroxyapatite crystals unpacked. Ultrastructural correlative analysis of the dentin in patients affected by OI showed an altered collagen pattern with a low density. In some areas, teeth in OI patients showed a reduction in the number of dentinal tubules, with odontoblastic process missing in most of the tubules. The presence of altered dentine and enamel organization in OI children was firmly established at an ultrastructural level, but additional biochemical studies are necessary in order to clarify quantitatively and qualitatively the collagenic and non-collagenic proteins in this disorder.

STRUCTURAL AND MORPHOLOGICAL CHARACTERIZATION OF GOLD NANOPARTICLES BY TEM AND DIGITAL CIS TECHNIQUE

2010 ◽  
Vol 09 (05) ◽  
pp. 399-406 ◽  
Author(s):  
A. A. EL-DALY
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Au Nanoparticles ◽  
Morphological Characterization ◽  
Particle Sizes ◽  
Rigid Surface ◽  
Gold Nanocrystals ◽  
Driving Mechanisms ◽  
Transmission Electron

In this paper, we report a convenient and informative procedure for detecting the morphology and surface structure of individual gold nanocrystals using digital Crystal Image Software (CIS) processing of transmission electron microscopy (TEM) image, which comprises coalescence phenomena of these nanoparticles. The results show that the internal structure of Au nanoparticles has a core of gold atoms arranged as a Marks decahedron, surrounded by additional gold–organic compound layers forming a rigid surface layer, and its outer layer comprises four staple motif bridge molecules that resemble handles, formed an unusual pattern. The obtained results improved our understanding of the basics of the coalescence phenomena such as the driving mechanisms acting at different particle sizes. However, these discrete natures of the nanoparticles will assist in the understanding of principles of nanocore assembly and opens a new window for nanoparticles chemistry.

scholarly journals Preparation and characterization of baicalein loaded phytosomes: Assessment of in vitro parameters

2020 ◽  
Vol 12 (4) ◽  
pp. 22-29
Author(s):  
KanchanV Zade ◽  
Alok Pal Jain
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Differential Scanning Calorimetry ◽  
Herbal Extracts ◽  
Scanning Calorimetry ◽  
Reverse Phase ◽  
Tem Analysis ◽  
Transmission Electron

Phytosome is a complex between natural active ingredient and a phospholipid. Further, phytosomes been applied to many popular herbal extracts or active molecules for augmenting oral dissolution. Therefore, in present investigation, orally administered Baicalein, atype of flavanoids, is poorly absorbed, and shows suboptimal dissolution. The phytosomes encapsulating baicalein (1:1 Mm) were prepared by reverse phase evaporation method followed by lyophilization. Transmission electron microscopy (TEM) analysis revealed that phytosomes were almost spherical in shape with particle size below 100 nm. The Powder ex-ray diffraction (PXRD) and differential scanning calorimetry (DSC) demonstrated that Baicalein loaded phytosomes were amorphous in nature. Amorphization of therapeutic moiety leads to improvement in dissolution. In conclusion, epigallocatechin loaded phytosomes exhibited promising results and warrant further in vitro andin vivo investigations under a set of stringent parameters for transforming in to a clinically viable products.

Low-Voltage Scanning Electron Microscopy Investigation of Polymers

1988 ◽  
Vol 46 ◽  
pp. 220-221
Author(s):  
V. K. Berry
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Low Voltage ◽  
Morphological Characterization ◽  
Transmission Electron ◽  
Conducting Materials ◽  
Voltage Scanning ◽  
Scanning Electron

The morphological characterization of any polymer blend plays an important part in the development of a new blend system because the properties of blends are dictated by phase morphology which is dependent upon the chemistry and the processing conditions. Light microscopy, scanning electron microscopy and transmission electron microscopy are the most commonly used microscopical techniques for morphological characterization. Transmission electron microscopy techniques provide the best resolution (≈ 0.3 nm) but are limited in the size of sample area and require elaborate sample preparation procedures. Surface charging and beam damage problems have been some of the drawbacks of conventional scanning electron microscopy with non-conducting materials like polymers.The use of low accelerating voltage scanning electron microscopy (LVSEM) in the characterization of polymers and other non-conducting materials is beginning to be recognized.

Synthesis and Electrical Characterization of Tin Oxide Nanostructures

2009 ◽  
Vol 1178 ◽  
Author(s):  
Olivia Maria Berengue ◽  
Cleocir J. Dalmaschio ◽  
Tiago G. Conti ◽  
Adenilson J. Chiquito ◽  
Edson R. Leite
Keyword(s):  
Electron Microscopy ◽  
Electrical Characterization ◽  
Morphological Characterization ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Current Voltage ◽  
Different Temperatures ◽  
Light Excitation ◽  
Resistance Curves

AbstractSn3O4 nanobelts were grown by a carbothermal evaporation process of SnO2 powders in association with the well known vapour-solid mechanism (VS). The nanobelts crystal structure was investigated by x-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM), raman spectroscopy and field emission gun scanning electron microscopy (FEG-SEM). The structural and morphological characterization has confirmed the growth of single crystal nanobelts. The electrical characterization (current-voltage, temperature-dependent resistance curves) of individual Sn3O4 nanobelts was performed at different temperatures and light excitation. The experiments revealed a semiconductor – like character as evidenced by the resistance decreasing at high temperatures. The transport mechanism was identified as the variable range hopping.

scholarly journals Morphological characterization of fullerene–androsterone conjugates

2014 ◽  
Vol 5 ◽  
pp. 374-379 ◽  
Author(s):  
Alberto Ruiz ◽  
Margarita Suárez ◽  
Nazario Martin ◽  
Fernando Albericio ◽  
Hortensia Rodríguez
Keyword(s):  
Electron Microscopy ◽  
Aqueous Solution ◽  
Transmission Electron Microscopy ◽  
Morphological Characterization ◽  
The Self ◽  
Self Organization ◽  
Fullerene Derivatives ◽  
Transmission Electron ◽  
Scale Structures

Here we report on the self-organization characteristics in water of two diastereomer pairs of fullerene–androsterone hybrids that have the hydrophobic C60 appendage in the A and D ring of the androsterone moiety, respectively. The morphology and particle size in aqueous solution were determined by transmission electron microscopy (TEM) and dynamic light scattering (DLS), with satisfactory agreement between both techniques. In general, these fullerene derivatives are shown to organize into spherical nano-scale structures with diameters in the ranges of 10–20 and 30–50 nm, respectively.

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