scholarly journals Ultrasound Extraction Mediated Recovery of Nutrients and Antioxidant Bioactive Compounds from Phaeodactylum tricornutum Microalgae

2021 ◽  
Vol 11 (4) ◽  
pp. 1701
Author(s):  
Fadila Al Khawli ◽  
Francisco J. Martí-Quijal ◽  
Noelia Pallarés ◽  
Francisco J. Barba ◽  
Emilia Ferrer
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Influential Factor ◽  
Extraction Time ◽  
Added Value ◽  
Antioxidant Compounds ◽  
Ultrasound Assisted Extraction ◽  
Processing Technologies ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In recent years, a growing interest has been shown in the use of microalgae due to their interesting nutritional and bioactive profiles. Green innovative processing technologies such as ultrasound-assisted extraction (UAE) avoid the use of toxic solvents and high temperatures, being a sustainable alternative in comparison with traditional extraction methods. The present study aims to evaluate the recovery of high added-value compounds from Phaedoactylum tricornutum assisted by ultrasound. To optimize the UAE of proteins, carbohydrates, pigments and antioxidant compounds, a response surface methodology was used. Carbohydrate extraction was positively affected by the temperature. However, for the extraction of carotenoids, the most influential factor was the extraction time. The total polyphenols were only significantly affected by the extraction time. Finally, the antioxidant capacity, measured by 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), was strongly modulated by the extraction time, while for the oxygen radical antioxidant capacity (ORAC) assay, the most important parameter was the temperature, followed by the extraction time. The optimal conditions for the maximum extraction of nutrients, bioactive compounds and antioxidant capacity were 30 min, 50 ºC and a pH of 8.5. Finally, it has been seen that with these conditions, the extraction of fucoxanthin is allowed, although no differences were found between an ultrasound-assisted extraction and a shaking extraction (control).

scholarly journals Optimization of Ultrasound-Assisted Extraction of Antioxidant Polyphenols from the Seed Coats of Red Sword Bean (Canavalia gladiate (Jacq.) DC.)

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 200 ◽  
Author(s):  
Yue Zhou ◽  
Xiao-Yu Xu ◽  
Ren-You Gan ◽  
Jie Zheng ◽  
Ya Li ◽  
...  
Keyword(s):  
Extraction Method ◽  
Natural Antioxidants ◽  
Extraction Time ◽  
Antioxidant Compounds ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Seed Coats

The seed coat of red sword bean (Canavalia gladiata (Jacq.) DC.) is rich in antioxidant polyphenols. It is often discarded as a byproduct with the consumption of red sword bean, since it is very thick and not consumed by people. The aim of this study was to develop an ultrasound-assisted extraction method to extract natural antioxidants from the seed coats. The extraction process was optimized by using response surface methodology. After the single-factor experiments, three key factors, including ethanol concentration, liquid/solid ratio, and extraction time, were selected and their interactions were studied using a central composite design. The optimal extraction condition was 60.2% hydroethanol, a liquid/solid ratio of 29.3 mL/g, an extraction time of 18.4 min, an extraction temperature of 50 °C, and ultrasound power of 400 W. Under the optimal conditions, antioxidant activity of the extract was 755.98 ± 10.23 μmol Trolox/g dry weight (DW), much higher than that from maceration (558.77 ± 14.42 μmol Trolox/g DW) or Soxhlet extraction (479.81 ± 12.75 μmol Trolox/g DW). In addition, the main antioxidant compounds in the extract were identified and quantified by high-performance liquid chromatography–diode array detection–tandem mass spectrometry (HPLC–DAD–MS/MS). The concentrations of digalloyl hexoside, methyl gallate, gallic acid, trigalloyl hexoside, and digallic acid were 15.30 ± 0.98, 8.85 ± 0.51, 8.76 ± 0.36, 4.27 ± 0.21, and 2.89 ± 0.13 mg/g DW. This study provides an efficient and green extraction method for the extraction of natural antioxidants from the bean coat of red sword bean. The extract of antioxidants might be added into functional foods or nutraceuticals with potential beneficial functions.

scholarly journals Green solvents and Ultrasound-Assisted Extraction of bioactive orange (Citrus sinensis) peel compounds

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Abigail Montero-Calderon ◽  
Clara Cortes ◽  
Ana Zulueta ◽  
Ana Frigola ◽  
Maria J. Esteve
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Orange Peel ◽  
Green Solvents ◽  
Ultrasound Assisted Extraction ◽  
Total Carotenoids ◽  
Carotenoid Concentration ◽  
Assisted Extraction ◽  
Physicochemical Evaluation ◽  
Ultrasound Assisted

Abstract Byproducts such as orange peel have potential uses because of their bioactive compounds, which are important for their potential to reduce the risk factors of diseases caused by aging. The lack of effective techniques and the high levels of pollution produced by the conventional extraction of bioactive compounds using organic solvents have highlighted the need to enhance the ‘green chemistry’ trend. This study evaluates the use of ultrasound to extract bioactive compounds from orange peel. The antioxidant capacity, phenolic content, ascorbic acid, total carotenoids, and HPLC profile of phenolic compounds from orange peel extracts were obtained by a physicochemical evaluation. The results demonstrate that the optimal conditions for the ultrasound-assisted extraction of bioactive orange peel compounds were a power of 400 W, a time of 30 min, and 50% ethanol in water. These conditions were used to obtain a total carotenoid concentration of 0.63 mg ß-carotene/100 g, vitamin C concentration of 53.78 mg AA/100 g, phenolic concentration of 105.96 mg GAE/100 g, and antioxidant capacity of ORAC = 27.08 mM TE and TEAC = 3.97 mM TE. The major phenolic compound identified in all orange peel extracts was hesperidin, with a maximum concentration of 113.03 ± 0.08 mg/100 g.

scholarly journals Optimization of ultrasound-assisted extraction of bioactive compounds from Gomphrena celosioides Mart. using response surface methodology

2020 ◽  
Vol 34 (2) ◽  
pp. 237-248
Author(s):  
N. T. H. Yen ◽  
L. P. T. Quoc
Keyword(s):  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Gomphrena Celosioides ◽  
Experimental Values

In this study, ultrasound-assisted extraction (UAE) was used to extract bioactive compounds from Gomphrena celosioides Mart. Central composite face design (CCF) was used to optimize the influences of extraction factors on total phenolics content (TPC), total flavonoids content (TFC), and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging capacity (DPPH-RSC). The results obtained showed that all factors (solvent to solid ratio, extraction time and extraction temperature) strongly affected TPC, TFC, and DPPH-RSC. The optimal extraction conditions for TPC (3.123 mg GAE/g DW), TFC (1.736 mg QE/g DW), and DPPH-RSC (64.118%) were solvent to solid ratio of 27.3/1 (mL/g), extraction time of 40.2 min, and extraction temperature of 69 oC. In addition, under the optimal conditions, the results pointed out that the experimental values agreed with those predicted. Hence, this model has successfully optimized the extraction conditions for TPC, TFC and DPPH-RSC of Gomphrena celosioides extract.                     KEY WORDS: Antioxidant activity, CCF, Flavonoids, Model, Phenolic compounds, Regression equation   Bull. Chem. Soc. Ethiop. 2020, 34(2), 237-248 DOI: https://dx.doi.org/10.4314/bcse.v34i2.3

scholarly journals Optimization of Ultrasound-Assisted Extraction of Bioactive Compounds from Acacia Seyal Gum Using Response Surface Methodology and Their Chemical Content Identification by Raman, FTIR, and GC-TOFMS

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1612
Author(s):  
Tahani Maher ◽  
Nassereldeen A. Kabbashi ◽  
Mohamed E. S. Mirghani ◽  
Md Z. Alam ◽  
Djabir Daddiouaissa ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Bioactive Compounds ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Acacia Seyal

Acacia Seyal gum (ASG), also known as gum Arabic, is an antioxidant-rich soluble fiber. ASG has been reported to have many biological activities, including anticancer, antidiabetic, antiulcer, and immunomodulatory activity. Extraction of bioactive compounds from ASG is commonly performed using conventional extraction methods. However, these techniques have certain limitation in terms of extraction time, energy, and solvent requirements. Ultrasound-assisted extraction (UAE) could be used as an alternative technique to extract bioactive compounds in less time, at low temperature, and with less energy and solvent requirements. In this study, the UAE extraction of ASG was optimized using response surface methodology (RSM). A face-centered central composite design (FCCCD) was used to monitor the effect of different independent factors of ultrasound operation (sonication time, temperature, and solvent ratio) on ASG extraction yield. In addition, screening and characterization of phytochemicals in 60% ethanol ASG extract was carried out using Raman microscopy, Fourier transform infrared spectroscopy (FTIR), and gas chromatography time-of-flight mass spectroscopy (GC-TOFMS) analysis. The results indicated that, under optimal conditions (extraction time 45 min, extraction temperature 40 °C, and solid–liquid ratio of 1:25 g/mL), the yield of ASG was 75.87% ± 0.10. This yield was reasonably close to the predicted yield of 75.39% suggested by the design of experiment. The ANOVA revealed that the model was highly significant due to the low probability value (p < 0.0001). Raman spectrum fingerprint detected polysaccharides, such as galactose and glucose, and protein like lysine and proline, while FTIR spectrum revealed the presence of functional groups peaks value of alkanes, aldehydes, aliphatic amines, and phenol. GC-TOFMS spectroscopic detected the presence of strong d-galactopyranose, carotenoid, and lycopene antioxidant compounds. In conclusion, this study demonstrated that the UAE technique is an efficient method to achieve a high yield of ASG extracts. The selected model is adequate to optimize the extraction of several chemical compounds reported in this study.

scholarly journals Critical Variables Influencing the Ultrasound-Assisted Extraction of Bioactive Compounds—A Review

2021 ◽  
Vol 5 (1) ◽  
pp. 50
Author(s):  
Anxo Carreira-Casais ◽  
Maria Carpena ◽  
Antia G. Pereira ◽  
Franklin Chamorro ◽  
Anton Soria-Lopez ◽  
...  
Keyword(s):  
Green Chemistry ◽  
Bioactive Compounds ◽  
Extraction Methods ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Extraction Performance ◽  
Ultrasound Power

Ultrasound-assisted extraction (UAE) is a novel methodology, belonging to the so-called “Green Chemistry”, which has gained interest in recent years due to the potential to recover bioactive compounds, especially those from plant matrices. It is widely recognized that the extraction of molecules by UAE gives rise to higher or similar yields than those obtained by traditional extraction methods. UAE has certain advantages inherent to Green Chemistry extraction methods, such as short extraction time and low solvent consumption. The aim of this review is to critically present the different variables and parameters that can be modified in UAE, such as ultrasound power, time, temperature, solvent, and solid to solvent ratio that influence yield and extraction performance.

scholarly journals Ultrasound-Assisted Extraction of Bioactive Compounds and Antioxidant Capacity for the Valorization of Elaeocarpus serratus L. Leaves

Processes ◽  
2020 ◽  
Vol 8 (10) ◽  
pp. 1218
Author(s):  
Ying-Hsuan Chen ◽  
Chun-Yao Yang
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Order Kinetic ◽  
Ultrasound Assisted Extraction ◽  
Sulfate Heptahydrate ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Elaeocarpus serratus L. leaves contain significant polyphenols, flavonoids, and myricitrin with medicinal property, which are byproducts after harvest and can be recovered for potential food applications. In this study, the effective extraction method for bioactive compounds and myricitrin from Elaeocarpus serratus L. leaves was investigated by comparing ultrasound-assisted extraction (UAE) and shaking-assisted extraction (SAE) for total phenolic content (TPC), total flavonoid content (TFC), myricitrin, and antioxidant capacity (as ferric reducing antioxidant power, FRAP). A first-order kinetic model of extraction was also proposed to correlate the observed extraction rate coefficient and limiting concentration of myricitrin in various solvents. Using defatted leaves (DL) as the substrate at the UAE condition of 95% ethanol with 1/10 (g/mL) of DL to solvent ratio under 40 kHz/300 W of ultrasound, 26.63% of yield of extract, and 199.75 mg-ferrous-sulfate-heptahydrate-equivalent/g-DL of FRAP were obtained. Further, the bioactive compounds extracted were 92.35 mg-gallic-acid-equivalent/g-DL of TPC, 53.14 mg-rutin-equivalent/g-DL of TFC, and 4.41 mg/g-DL of myricitrin, which were 1.53, 1.44, and 1.4 folds using SAE at 120 rpm, respectively. This showed that UAE was more efficient than SAE in bioactive compounds recovery for valorization of Elaeocarpus serratus L. leaves.

scholarly journals Ultrasound-Assisted Extraction of GAC Peel: An Optimization of Extraction Conditions for Recovering Carotenoids and Antioxidant Capacity

Processes ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 8 ◽  
Author(s):  
Hoang V. Chuyen ◽  
Paul D. Roach ◽  
John B. Golding ◽  
Sophie E. Parks ◽  
Minh H. Nguyen
Keyword(s):  
Antioxidant Capacity ◽  
Extraction Time ◽  
Carotenoid Content ◽  
Ultrasonic Power ◽  
Optimal Conditions ◽  
Total Carotenoid ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
The Impact

The peel of Gac fruit (Momordica cochinchinensis Spreng.), which is considered as waste of Gac processing, has been found to possess high levels of carotenoids and other antioxidants. This study aimed at determining the optimal conditions of an ultrasound-assisted extraction for recovering carotenoids and antioxidant capacity from Gac peel. A response surface methodology using the Box–Behnken design was employed to investigate the impact of extraction time, temperature and ultrasonic power on the recovery of total carotenoid and antioxidant capacity. The results showed that an extraction time of 76 min, temperature of 50 °C and ultrasonic power of 250 W were the optimal conditions for the extraction. The experimental carotenoid yield and antioxidant capacity obtained under the optimal extraction conditions were validated as 269 mg/100 g DW (dry weight) and 822 µM TE (Trolox equivalent)/100 g DW, respectively. These values were not significantly different from the values predicted by the models. The HPLC analysis for carotenoid composition showed that β-carotene, lycopene and lutein were the principal carotenoids of the extract, which constitute 86% of the total carotenoid content. Based on the obtained results, the ultrasound-assisted extraction using ethyl acetate under the above optimal conditions is suggested for the simultaneous recovery of carotenoids and antioxidant capacity from Gac peel.

scholarly journals Effect of Ultrasound-Assisted Extraction Parameters on Total Polyphenols and Its Antioxidant Activity from Mango Residues (Mangifera indica L. var. Manililla)

Separations ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 94
Author(s):  
Anahí J. Borrás-Enríquez ◽  
Elizabeth Reyes-Ventura ◽  
Socorro J. Villanueva-Rodríguez ◽  
Lorena Moreno-Vilet
Keyword(s):  
Antioxidant Activity ◽  
Mangifera Indica ◽  
Central Point ◽  
Added Value ◽  
Sonication Time ◽  
Ultrasound Assisted Extraction ◽  
Extraction Technology ◽  
Total Polyphenols ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Manililla is a mango variety whose residues contain bioactive compounds such as polyphenols and flavonoids, with high added value. The use of environmentally friendly extraction technology would be of great relevance; hence, this study aimed to evaluate the effect of solvent relation, sonication time and amplitude on the ultrasound-assisted extraction of total polyphenols in Manililla mango residues (peel, endocarp and kernel) and antioxidant activity. An experimental design 23 with a central point was used to evaluate the curvature behavior of the process variables. Conventional maceration was used as a control. The better conditions were obtained at the central point using 50% ethanol in water, 60% amplitude and 20 min of sonication time. We obtained values of up to 1814 mg GAE/100 g, 469 mg GAE/100 g and 672 mg GAE/100 g of total polyphenols and 1228 mg QE/100 g, 653 mg QE/100 g and 880 mg QE/100 g of total flavonoids for peel, endocarp and kernel, respectively. Mangiferin was quantified in ultrasound-assisted extraction at 150 mg/g in peel and 0.025 mg/g in the kernel, but it was not detectable in maceration. An antioxidant capacity of 87%, 14% and 83% inhibition for peel, endocarp and kernel, respectively, were obtained. Peel and kernel were the residues with higher potential as extraction material, while endocarp was not.

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