Entomopathogenic Fungi Biomass Production and Extracellular Biosynthesis of Silver Nanoparticles for Bioinsecticide Action

Entomopathogenic fungi are microbial agents of insect control in nature. They have been used as biologic strategies to manage insect invasion; however, the challenge is to maintain their shelf life and viability when exposed to high temperatures, ultraviolet radiation, and humidity. Synthesized silver nanoparticles (AgNPs) from fungal extracellular enzymes are an alternative using these microorganisms to obtain nanoparticles with insecticidal action. The present study evaluates the biomass production and the potential to synthesize silver nanoparticles using entomopathogenic fungi isolates. Sixteen isolates of entomopathogenic fungi were used in this study. The fungi pathogenicity and virulence were evaluated using the insect model Tenebrio molitor, at a concentration of 5 × 106 conidia/mL. The fungal biomass was produced in a liquid medium, dried, and weighed. The synthesis of silver nanoparticles was performed with aqueous extracts of the entomopathogenic fungi and silver nitrate solution (1 mM), following characterization by a UV/vis spectrophotometer, mean size, and polydispersity index. The results showed a significant variation in pathogenicity, virulence, and biomass production among the evaluated fungi isolates; however, only one of the isolates did not have the potential to synthesize silver nanoparticles. Pearson’s correlation showed significant correlation values only between virulence × biosynthesis potential and biomass production × biosynthesis potential, both with negative values, indicating an inverse correlation. Thus, AgNPs with entomopathogenic fungus extract can produce an innovative bioinsecticide product using a green production process.

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Sintesis dan karakterisai kitosan dari limbah cangkang udang sebagai stabilizer terhadap Ag nanopartikel

Fullerene Journal of Chemistry ◽

10.37033/fjc.v4i2.100 ◽

2019 ◽

Vol 4 (2) ◽

pp. 86

Author(s):

Astuti Amin ◽

Nur Khairi ◽

Eko Allo

Keyword(s):

Silver Nanoparticles ◽

Chemical Reduction ◽

Nitrate Solution ◽

Average Particle Size ◽

Silver Nitrate Solution ◽

Raw Material ◽

Average Particle ◽

Synthesize Silver Nanoparticles ◽

Shrimp Shell ◽

Shell Powder

The research of manufacturing chitosan from shrimp shell waste , and their use as a stabilizer in the manufacture of silver nanoparticles has been done. The aim of the research was to synthesize silver nanoparticles using chitosan as a stabilizer by chemical reduction method and determine the effect of chitosan concentration on the stability of Ag nanoparticles. In this study, the raw material used is shrimp shell powder and then processed in several stages, eliminating proteins, demineralization, and deacetylation. Chitosan obtained is 16.4 % of shrimp shell powder, with a degree of deacetylation of 85 %. Chitosan is used to synthesize silver nanoparticles as a reducing agent of silver ions in silver nitrate solution and is expected to be stabilizer. Sample containing 45 mg of chitosan and 1000 ppm AgNO3 has 421,60 nm of maximum wavelength, and the average particle size is 154.07 nm.

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Microwave-assisted green synthesis of silver nanoparticles using dried extracts of Chlorella vulgaris and antibacterial activity studies

Green Processing and Synthesis ◽

10.1515/gps-2020-0024 ◽

2020 ◽

Vol 9 (1) ◽

pp. 283-293

Author(s):

Milad Torabfam ◽

Meral Yüce

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Chlorella Vulgaris ◽

Metallic Nanoparticles ◽

Nitrate Solution ◽

Spherical Shape ◽

Silver Nitrate Solution ◽

Spectroscopy Analysis ◽

Bacterial Diseases ◽

Serovar Typhimurium

AbstractGreen synthesis of metallic nanoparticles (NPs) is acquiring considerable attention due to its environmental and economic superiorities over other methods. This study describes the practical synthesis of silver nanoparticles (AgNPs) through the reduction of silver nitrate solution using an algal source, Chlorella vulgaris, as the reducing as well as the stabilizing agent. The energy required for this synthesis was supplied by microwave radiation. The ultraviolet-visible spectroscopy exhibited a single peak related to the surface plasmon absorbance of AgNPs at 431 nm. The AgNPs with high stability (a zeta potential of −17 mV), hydrodynamic size distribution of 1–50 nm, and mostly spherical shape were obtained through a 10 min process. Fourier transform infrared spectroscopy analysis revealed that several functional groups, including carbonyl groups of C. vulgaris, play a significant role in the formation of functional NPs. Antibacterial features of the produced AgNPs were verified against those of Salmonella enterica subsp. enterica serovar typhimurium and Staphylococcus aureus, demonstrating a considerable growth inhibition at increasing concentrations of the NPs. As a result, the formed AgNPs can be used as a promising agent against bacterial diseases.

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Antimicrobial Bacterial Cellulose-Silver Nanoparticles Composite Membranes

Journal of Nanomaterials ◽

10.1155/2011/721631 ◽

2011 ◽

Vol 2011 ◽

pp. 1-8 ◽

Cited By ~ 117

Author(s):

Hernane S. Barud ◽

Thaís Regiani ◽

Rodrigo F. C. Marques ◽

Wilton R. Lustri ◽

Younes Messaddeq ◽

...

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Bacterial Cellulose ◽

Nitrate Solution ◽

Composite Membranes ◽

Silver Nitrate Solution ◽

Complexing Agent ◽

X Ray Diffraction ◽

Gram Positive Bacteria ◽

Transmission Electron

Antimicrobial bacterial cellulose-silver nanoparticles composite membranes have been obtained by“in situ”preparation of Ag nanoparticles from hydrolytic decomposition of silver nitrate solution using triethanolamine as reducing and complexing agent. The formation of silver nanoparticles was evidenced by the X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and absorption in the UV-Visible (350 nm to 600 nm). Thermal and mechanical properties together with swelling behavior for water were considered. TEA concentration was observed to be important in order to obtain only Ag particles and not a mixture of silver oxides. It was also observed to control particle size and amount of silver contents in bacterial cellulose. The composite membranes exhibited strong antimicrobial activity against Gram-negative and Gram-positive bacteria.

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Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Produced by Yarrowia lipolytica Against Vegetative and Starved Shigella

NANO ◽

10.1142/s1793292021500880 ◽

2021 ◽

pp. 2150088

Author(s):

Kalthoum Chourabi ◽

Lobna Elleuch ◽

Salma Kloula ◽

Ahmed Landoulsi ◽

Abdelwaheb Chatti

Keyword(s):

Silver Nanoparticles ◽

Yarrowia Lipolytica ◽

Nitrate Solution ◽

Silver Nitrate Solution ◽

Water Disinfection ◽

Personal Hygiene ◽

Visible Range ◽

Significant Toxicity ◽

Air Disinfection ◽

The Stability

Silver nanoparticles have attracted much interest from scientists to develop nanosilver-based disinfectant products due to their unique properties of high antimicrobial activity. This study focused on biosynthesis, characterization, antimicrobial and antibiofilm effects of silver nanoparticles against vegetative and starved Shigella strains. The silver nanoparticles were synthesized using the yeast Yarrowia lipolytica and characterized by ultraviolet–visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The antimicrobial and antibiofilm activities of silver nanoparticles were tested against the growth of vegetative and starved Shigella strains. After the addition of silver nitrate solution to the supernatant of Y. lipolytica, we noticed the appearance of a brown-black coloration that suggested the formation of silver nanoparticles. The presence of silver nanoparticles was manifested by a maximum absorption in the ultraviolet–visible range, precisely at the wavelength 420[Formula: see text]nm. The crystalline nature and the stability of silver nanoparticles were confirmed, respectively, by XRD and FTIR analysis. The antibacterial activity of silver nanoparticles showed significant toxicity on Shigella strains indicating that the starved cells were more sensitive to treatment with silver nanoparticles than vegetative cells. Surprisingly, the biofilm formation had not been inhibited by silver nanoparticles for both vegetative and starved cells. In conclusion, a new class of nanosilver containing disinfectant nanoproducts will be promising for advanced environmental treatments including air disinfection, water disinfection, surface disinfection and personal hygiene that will help to prevent the further outbreak of diseases.

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Controlling the Surface Plasmon Absorption of Silver Nanoparticles via Green Synthesis Using Pennisetum purpureum Leaf Extract

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.772.73 ◽

2018 ◽

Vol 772 ◽

pp. 73-77

Author(s):

Ruelson S. Solidum ◽

Arnold C. Alguno ◽

Rey Capangpangan

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Silver Nitrate ◽

Surface Plasmon ◽

Leaf Extract ◽

Nitrate Solution ◽

Silver Nitrate Solution ◽

Absorption Peak ◽

Plasmon Absorption ◽

Surface Plasmon Absorption

We report on the green synthesis of silver nanoparticles utilizing theP.purpureumleaf extract. Controlling the surface plasmon absorption of silver nanoparticles was achieved by regulating the amount of extract concentration and the molarity of silver nitrate solution. The surface plasmon absorption peak is found at around 430nm. The surface plasmon absorption peak have shifted to lower wavelength as the amount of extract is increased, while plasmon absorption peak shifts on a higher wavelength as the concentration of silver nitrate is increased before it stabilized at 430nm. This can be explained in terms of the available nucleation sites promoted by the plant extract as well as the available silver ions present in silver nitrate solution.

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The Synthesis and Stability Study of Silver Nanoparticles Prepared by Using p-Aminobenzoic Acid as Reducing and Stabilizing Agent

Indonesian Journal of Chemistry ◽

10.22146/ijc.29312 ◽

2018 ◽

Vol 18 (3) ◽

pp. 421 ◽

Cited By ~ 2

Author(s):

Dian Susanthy ◽

Sri Juari Santosa ◽

Eko Sri Kunarti

Keyword(s):

Silver Nanoparticles ◽

Reaction Time ◽

Silver Nitrate ◽

Tap Water ◽

Nitrate Solution ◽

Silver Nitrate Solution ◽

Stability Study ◽

Synthesis Process ◽

Aminobenzoic Acid ◽

Stabilizing Agent

A study to examine the performance of p-aminobenzoic acid as both reducing agent for silver nitrate to silver nanoparticles (AgNPs) and stabilizing agent for the formed AgNPs has been done. The synthesis of AgNPs was performed by mixing silver nitrate solution as precursor with p-aminobenzoic acid solution and heating it in a boiling water bath. After the solution turned to yellow, the reaction stopped by cooling it in tap water. The formed AgNPs were analyzed by using UV-Vis spectrophotometry to evaluate their SPR absorption in wavelength range of 400–500 nm. The synthesis process was highly depend on the pH, reaction time, and mole ratios of the reactants. The synthesis only occur in pH 11 and at reaction time 30 min, the particle size of the formed AgNPs was 12 ± 7 nm. Longer reaction time increased the reducing performance of p-aminobenzoic acid in AgNPs synthesis but decreased its stabilizing performance. The increase of silver nitrate amount relative to p-aminobenzoic acid in the synthesis increased the reducing and stabilizing performance of p-aminobenzoic acid and the optimum mole ratio between AgNO3 and p-aminobenzoic acid was 5:100 (AgNO3 to p-aminobenzoic acid).

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Green Synthesis of Silver Nanoparticles Using Melia Azedarach and its Characterization, Corrosion and Antibacterial Properties

Biointerface Research in Applied Chemistry ◽

10.33263/briac111.85778586 ◽

2020 ◽

Vol 11 (1) ◽

pp. 8577-8586

Keyword(s):

Silver Nanoparticles ◽

Electron Microscope ◽

Color Change ◽

Nitrate Solution ◽

Antibacterial Properties ◽

Nanoparticle Synthesis ◽

Silver Nitrate Solution ◽

Xrd Analysis ◽

Melia Azedarach ◽

Change State

Nanoparticle synthesis by the biological method is economical and environmentally friendly. In the present study, the biosynthesis of silver nanoparticles is performed by using extracts of Melia Azedarach plant leaves. The synthesis is performed by adding to the silver nitrate solution to the leaf extract of Melia azedarach. The color change state the precipitation of nanoparticles of silver. The silver nanoparticles obtained were characterized by various techniques such as X-ray diffraction (XRD), Scanning electron microscope (SEM), transmission electron microscope (TEM), and Energy dispersive spectroscopy (EDX). The XRD analysis shows the particle size of 11 nm calculated by the Debye-Scherrer. The microstructure analysis shows silver particles of spherical and triangular. The corrosion rate was found to be 0.025 mpy. The green silver nanoparticle (AgNPs) synthesized have better antimicrobial potential against both bacteria’s (Bacillus subtilis and Pseudomonas aeruginosa).

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Pretreated Lignocellulosic Waste Mediated Biosynthesis of Silver Nanoparticles Under Room Temperature

International Journal of Nanoscience ◽

10.1142/s0219581x16600012 ◽

2016 ◽

Vol 15 (05n06) ◽

pp. 1660001 ◽

Cited By ~ 1

Author(s):

V. P. Manjamadha ◽

Karuppan Muthukumar

Keyword(s):

Silver Nanoparticles ◽

Silver Nitrate ◽

Room Temperature ◽

Nitrate Solution ◽

Silver Ions ◽

Silver Nitrate Solution ◽

Morphological Characterization ◽

Alternative Source ◽

X Ray ◽

Antibacterial Performance

The current work elucidates the utilization of biowaste as a valuable reducing agent for the synthesis of silver nanoparticles. In this study, the wastewater generated during the alkaline pretreatment of lignocellulosic wastes (APLW) was used as a bioreductant to reduce silver nitrate under room temperature. Synthesis of stable silver nanoparticles (AgNPs) was achieved rapidly on addition of APLW into the silver nitrate solution (1[Formula: see text]mM). The morphological characterization of AgNPs was performed using field emission scanning electron microscopy (FESEM). The micrograph clearly depicted the presence of spherical AgNPs. The presence of elemental silver along with biomoilties was determined using energy dispersive X-ray spectroscopy (EDAX) analysis. The X-ray diffraction (XRD) study proved the crystalline form of stable AgNPs. The AgNPs exhibited excellent antibacterial performance against Gram negative organism. The immediate bioreduction of silver ions using APLW was well illustrated in the present study. Thus, APLW serve as an alternative source for reducing agents instead of utilizing valuable medicinal plants for nanoparticles synthesis.

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Synthesis and Characterization of Silver Nanoparticles from Couroupita guianensis leaf extract–A Simple Green Route

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00493 ◽

2021 ◽

pp. 2796-2800

Author(s):

Inbathamizh L ◽

Kalpana V ◽

Revathi Yadav K

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Nitrate Solution ◽

Ultra Violet ◽

Silver Nitrate Solution ◽

Green Route ◽

Couroupita Guianensis ◽

Potential Applications ◽

Aqueous Leaf Extract

With the increase in the potential applications of nanoparticles in pharma and various fields, nanoparticle research is attracting more attention. Though several chemical and physical methods are being used for the synthesis of metal nanoparticles, they are associated with several disadvantages. Couroupita guianensis is a traditional plant with medicinal values. The focus of the study was to follow a green chemistry route to synthesize silver nanoparticles (AgNPs) using the leaf extract of Couroupita guianensis as a reductant and stabilizing agent. The boiled aqueous leaf extract with silver nitrate solution on exposure to sunlight showed the maximum absorbance at 430nm indicating the synthesis of AgNPs. Ultra Violet (UV)-Visible spectroscopy, Scanning Electron Microscopy (SEM), and Fourier Transform Infrared spectroscopy (FTIR) techniques were used for the characterization of AgNPs. The synthesized AgNPs were found to be spherical and 4.44 – 40.20nm in size. They also seemed to be capped with the significant functional groups present in the leaf extract. Thus, the study suggested Couroupita guianensis mediated green synthesis of AgNPs as an efficient and eco-friendly approach with substantial advantages over the conventional methods. The process could be further scaled-up for mass production and wider applications of AgNPs.

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Degradation of Methylene Blue Using Biologically Synthesized Silver Nanoparticles

Bioinorganic Chemistry and Applications ◽

10.1155/2014/742346 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 81

Author(s):

M. Vanaja ◽

K. Paulkumar ◽

M. Baburaja ◽

S. Rajeshkumar ◽

G. Gnanajobitha ◽

...

Keyword(s):

Methylene Blue ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Nitrate Solution ◽

Reaction Medium ◽

Silver Ions ◽

Silver Nitrate Solution ◽

X Ray ◽

Synthesis Of Nanoparticles ◽

Aqueous Leaf Extract

Nowadays plant mediated synthesis of nanoparticles has great interest and achievement due to its eco-benign and low time consuming properties. In this study silver nanoparticles were successfully synthesized by usingMorinda tinctorialeaf extract under different pH. The aqueous leaf extract was added to silver nitrate solution; the color of the reaction medium was changed from pale yellow to brown and that indicates reduction of silver ions to silver nanoparticles. Thus synthesized silver nanoparticles were characterized by UV-Vis spectrophotometer. Dispersity and morphology was characterized by scanning electron microscope (SEM); crystalline nature and purity of synthesized silver nanoparticles were revealed by X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). FTIR spectrum was examined to identify the effective functional molecules responsible for the reduction and stabilization of silver nanoparticles synthesized by leaf extract. The photocatalytic activity of the synthesized silver nanoparticles was examined by degradation of methylene blue under sunlight irradiation. Green synthesized silver nanoparticles were effectively degrading the dye nearly 95% at 72 h of exposure time.

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