scholarly journals Single-Stage Fractionation of Vine Shoots Using Microwave Heating

2021 ◽  
Vol 11 (17) ◽  
pp. 7954
Author(s):  
Sandra Rivas ◽  
Juan Carlos Parajó
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aqueous Phase ◽  
Solid Phase ◽  
Degree Of Polymerization ◽  
Agricultural Residues ◽  
Single Stage ◽  
Raw Material ◽  
Food Ingredients ◽  
Stage Separation ◽  
Hydrolysis Of

Vine shoots are agricultural residues that can be used as a raw material in agro-biorefineries, in which their main constituents can be individually converted into valuable bioproducts. The treatment of vine shoots in uncatalyzed media containing water and 1-butanol enabled the single-stage separation of the major vine shoots constituents in different phases: the aqueous phase from treatments contained hemicellulose-derived products (mainly in the form of oligosaccharides), the organic phase accumulated the dissolved lignin, and the cellulosic fraction was recovered in solid phase. The aqueous phase from treatments was refined using membranes and processed with enzymes to obtain a refined product (RP) containing 92.2 g of oligosaccharides/100 g of non-volatile compounds. The oligosaccharides were mainly composed of anhydroxylose units substituted by acetyl and uronic groups. Enzymatic hydrolysis of RP with endo-xylanases reduced the average degree of polymerization to 2-3, which are preferred for application as healthy food ingredients. The solid phase from treatments was used as a substrate for enzymatic hydrolysis, enabling the production of solutions containing 34.9 g glucose /L and 4.2 g xylose /L.

Improving the efficiency of enzymatic hydrolysis of Eucalyptus residues with a modified aqueous ammonia soaking method

2018 ◽  
Vol 33 (2) ◽  
pp. 165-174 ◽  
Author(s):  
Dan Huo ◽  
Qiulin Yang ◽  
Guigan Fang ◽  
Qiujuan Liu ◽  
Chuanling Si ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Hydrolysis ◽  
Aqueous Ammonia ◽  
Pulp Mill ◽  
Fiber Surface ◽  
Raw Material ◽  
Sem Images ◽  
Aqueous Ammonia Soaking ◽  
Guaiacyl Lignin ◽  
Hydrolysis Of

Abstract Eucalyptus residues from pulp mill were pretreated with aqueous ammonia soaking (AAS) method to improve the efficiency of enzymatic hydrolysis. The optimized condition of AAS was obtained by response surface methodology. Meanwhile, hydrogen peroxide was introduced into the AAS system to modify the AAS pretreatment (AASP). The results showed that a fermentable sugar yield of 64.96 % was obtained when the eucalypt fibers were pretreated at the optimal conditions, with 80 % of ammonia (w/w) for 11 h and keeping the temperature at 90 °C. In further research it was found that the addition of H2O2 to the AAS could improve the pretreatment efficiency. The delignification rate and enzymatic digestibility were increased to 64.49 % and 73.85 %, respectively, with 5 % of hydrogen peroxide being used. FTIR analysis indicated that most syringyl and guaiacyl lignin and a trace amount of xylan were degraded and dissolved during the AAS and AASP pretreatments. The CrI of the raw material was increased after AAS and AASP pretreatments, which was attributed to the removal of amorphous portion. SEM images showed that microfibers were separated and explored from the initial fiber structure after AAS pretreatment, and the AASP method could improve the destructiveness of the fiber surface.

Optimization of steam pretreatment conditions for enzymatic hydrolysis of poplar wood

Holzforschung ◽  
2011 ◽  
Vol 65 (4) ◽  
Author(s):  
Fokko Schütt ◽  
Jürgen Puls ◽  
Bodo Saake
Keyword(s):  
Enzymatic Hydrolysis ◽  
Raw Material ◽  
Steam Pretreatment ◽  
Alkaline Extraction ◽  
Poplar Wood ◽  
Steam Refining ◽  
Severity Factor ◽  
Short Rotation ◽  
Pretreatment Conditions ◽  
Hydrolysis Of

Abstract Steam refining was investigated as a pretreatment for enzymatic hydrolysis of poplar wood from a short rotation plantation. The experiments were carried out without debarking to use an economically realistic raw material. Steam refining conditions were varied in the range of 3–30 min and 170–220°C, according to a factorial design created with the software JMP from SAS Institute Inc., Cary, NC, USA. Predicted steaming conditions for highest glucose and xylose yields after enzymatic hydrolysis were at 210°C and 15 min. Control tests under the optimized conditions verified the predicted results. Further pretreatments without bark showed that the enzymes were not significantly inhibited by the bark. The yield of glucose and xylose was 61.9% of theoretical for the experiments with the whole raw material, whereas the yield for the experiments without bark was 63.6%. Alkaline extraction of lignin from the fibers before enzymatic hydrolysis resulted in an increase of glucose yields from mild pretreated fibers and a decrease for severe pretreated fibers. The extracted lignin had a high content of xylose of up to 14% after very mild pretreatments. On the other hand, molecular weights of the extracted lignin increased substantially after pretreatments with a severity factor above 4. Hence, alkaline extraction of the lignin seems only attractive in a narrow range of steaming conditions.

scholarly journals A Novel Inulin-Mediated Ethanol Precipitation Method for Separating Endo-Inulinase From Inulinases for Inulooligosaccharides Production From Inulin

2021 ◽  
Vol 9 ◽  
Author(s):  
Xin Li ◽  
Qiannan Zhang ◽  
Wei Wang ◽  
Shang-Tian Yang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Functional Food ◽  
Degree Of Polymerization ◽  
Precipitation Method ◽  
High Yield ◽  
Process Conditions ◽  
Food Ingredients ◽  
Ethanol Precipitation ◽  
Novel Method

Inulin is a kind of polysaccharide that can be obtained various biomass. Inulooligosaccharides (IOS), a kind of oligosaccharides that can be obtained from inulin by enzymatic hydrolysis using inulinases, have been regarded as the functional food ingredients. Commercially available inulinases produced by natural Aspergillus niger contained both endo- and exo-inulinase activities. For IOS production from inulin, it is desirable to use only endo-inulinase as exo-inulinase would produce mainly the monosacchairde fructose from inulin. In the present study, a simple inulin-mediated ethanol precipitation method was developed to separate endo- and exo-inulinases present in natural inulinases. IOS production from inulin using the enriched endo-inulinase was then optimized in process conditions including pH and temperature, achieving a high yield of ∼94%. The resultant IOS products had a degree of polymerization ranging from 2 to 7. The study demonstrated a novel method for obtaining partially purified or enriched endo-inulinase for IOS production from inulin in an efficient process.

scholarly journals Calcium peroxide pretreatment to facilitate the delignification and enzymatic hydrolysis of wheat straw

2021 ◽  
Author(s):  
Youshan Sun ◽  
Xuyang Zhang ◽  
Fei Wang ◽  
Meiyan Wang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Complex Structure ◽  
Raw Material ◽  
Liquid Ratio ◽  
Enzymatic Digestibility ◽  
Calcium Peroxide ◽  
Glucose Recovery ◽  
Solid Liquid ◽  
Hydrolysis Of

Abstract Calcium peroxide (CaO2) pretreatment was employed to remove lignin and subsequently facilitate enzymatic digestibility of wheat straw. An optimal condition was obtained at 130°C for 10 min with 0.35 g CaO2/g dried material of wheat straw and a 1:8 solid-liquid ratio. Under this condition, 57.8% of initial lignin, 7.2% of initial glucan, and 30.6% of initial xylan were removed from CaO2 pretreatment, respectively, meanwhile, a glucose recovery of 90.6 % and a xylose recovery of 65.9 % were obtained from the subsequent enzymatic hydrolysis of treated wheat straw, respectively. CaO2 pretreatment was proved to be a very effective method in delignification and improving enzymatic digestibility. Compared to raw material, the complex structure of lignocellulose was drastically disrupted with a wide emergence of scaly bulges and fully exposed microfibers, which still retained in the solid.

scholarly journals Pretreatments of Non-Woody Cellulosic Feedstocks for Bacterial Cellulose Synthesis

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1645 ◽  
Author(s):  
Ekaterina I. Kashcheyeva ◽  
Yulia A. Gismatulina ◽  
Vera V. Budaeva
Keyword(s):  
Enzymatic Hydrolysis ◽  
Bacterial Cellulose ◽  
High Performance ◽  
Combined Treatment ◽  
Single Stage ◽  
High Tech ◽  
Cellulose Synthesis ◽  
Oat Hulls ◽  
Naoh Solution ◽  
Hydrolysis Of

Pretreatment of biomass is a key step in the production of valuable products, including high-tech bacterial cellulose. The efficiency of five different pretreatment methods of Miscanthus and oat hulls for enzymatic hydrolysis and subsequent synthesis of bacterial cellulose (BC) was evaluated herein: Hydrothermobaric treatment, single-stage treatments with dilute HNO3 or dilute NaOH solution, and two-stage combined treatment with dilute HNO3 and NaOH solutions in direct and reverse order. The performance of enzymatic hydrolysis of pretreatment products was found to increase by a factor of 4−7. All the resultant hydrolyzates were composed chiefly of glucose, as the xylose percentage in total reducing sugars (RS) was 1−9%. The test synthesis of BC demonstrated good quality of nutrient media prepared from all the enzymatic hydrolyzates, except the hydrothermobaric treatment hydrolyzate. For biosynthesis of BC, single-stage pretreatments with either dilute HNO3 or dilute NaOH are advised due their simplicity and the high performance of enzymatic hydrolysis of pretreatment products (RS yield 79.7−83.4%).

scholarly journals Enzymatic Hydrolysis of Water Extractable Polysaccharides from Leaves of Plantago major L.

Folia Medica ◽  
2017 ◽  
Vol 59 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Paolina K. Lukova ◽  
Diana P. Karcheva-Bahchevanska ◽  
Veselin P. Bivolarski ◽  
Rumen D. Mladenov ◽  
Ilia N. Iliev ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Degree Of Polymerization ◽  
Hot Water ◽  
Cell Mediated Immunity ◽  
Plantago Major ◽  
Beneficial Effects ◽  
Hydrolysis Yield ◽  
Antiviral Activities ◽  
Hydrolysis Of ◽  
Water Extractable

AbstractBackground:Plantago majorL. leaves have been used for centuries by the traditional medicine in the treatment of infectious disorders of the respiratory, urinary and digestive tracts. Researchers have reported that hot water extracts ofPlantago majorpossess a broad-spectrum of anticancer, antioxidant and antiviral activities, as well as activities which modulate cell-mediated immunity. Their beneficial properties may be due to the significant content of polysaccharides. The polysaccharides that have been isolated from the leaves ofPlantago majorL. have different structures – pectic substances, galactans, arabinogalactans, glucomannans.Aim:The aim of this paper was to study the correlation between the structure of the water extractable polysaccharides isolated fromPlantago majorL. leaves and their enzymatic hydrolysis with different carbohydrate hydrolases.Materials and methods:The hydrolysis reactions were performed with the enzymes hemicellulase and mannanase. Spectrophotometric total reducing sugars assay was used to examine the hydrolysis yield. The monosaccharide and oligosaccharide compositions were determined using HPLC analysis.Results:The highest hydrolysis yield of the water extractable polysaccharides fromPlantago majorleaves was obtained by treatment with hemicellulase. The hydrolysis yield increased with the augmentation of the ratio of enzyme to polysaccharide. Galactose was the prevalent monosaccharide identified in the composition of the isolated polysaccharides. Oligosaccharides with different degree of polymerization were also detected.Conclusion:The enzymatic hydrolysis of water extractable polysaccharides fromPlantago majorleaves allows us to obtain different types of oligosaccharides with beneficial effects on both human health and industry.

scholarly journals Enhanced Enzymatic Hydrolysis of Corncob by Synthesized Enzyme-Mimetic Magnetic Solid Acid Pretreatment in an Aqueous Phase

ACS Omega ◽  
2019 ◽  
Vol 4 (18) ◽  
pp. 17864-17873 ◽  
Author(s):  
Qing Xu ◽  
Wei Yang ◽  
Guifeng Liu ◽  
Cuiyi Liang ◽  
Si Lu ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aqueous Phase ◽  
Solid Acid ◽  
Acid Pretreatment ◽  
Enzyme Mimetic ◽  
Magnetic Solid ◽  
Magnetic Solid Acid ◽  
Hydrolysis Of

scholarly journals Co-production of Xylooligosaccharides and Xylose From Poplar Sawdust by Recombinant Endo-1,4-β-Xylanase and β-Xylosidase Mixture Hydrolysis

2021 ◽  
Vol 8 ◽  
Author(s):  
Qi Li ◽  
Yunpeng Jiang ◽  
Xinyi Tong ◽  
Linguo Zhao ◽  
Jianjun Pei
Keyword(s):  
Enzymatic Hydrolysis ◽  
Deep Understanding ◽  
Important Application ◽  
Raw Material ◽  
Hydrolysis Yield ◽  
Wood Polysaccharides ◽  
Optimized Conditions ◽  
Rate Limiting ◽  
Hydrolysis Of ◽  
Poplar Sawdust

As is well-known, endo-1,4-β-xylanase and β-xylosidase are the rate-limiting enzymes in the degradation of xylan (the major hemicellulosic component), main functions of which are cleavaging xylan to release xylooligosaccharides (XOS) and xylose that these two compounds have important application value in fuel, food, and other industries. This study focuses on enzymatic hydrolysis of poplar sawdust xylan for production of XOS and xylose by a GH11 endo-1,4-β-xylanase MxynB-8 and a GH39 β-xylosidase Xln-DT. MxynB-8 showed excellent ability to hydrolyze hemicellulose of broadleaf plants, such as poplar. Under optimized conditions (50°C, pH 6.0, dosage of 500 U/g, substrate concentration of 2 mg/mL), the final XOS yield was 85.5%, and the content of XOS2−3 reached 93.9% after 18 h. The enzymatic efficiency by MxynB-8 based on the poplar sawdust xylan in the raw material was 30.5%. Xln-DT showed excellent xylose/glucose/arabinose tolerance, which is applied as a candidate to apply in degradation of hemicellulose. In addition, the process and enzymatic mode of poplar sawdust xylan with MxynB-8 and Xln-DT were investigated. The results showed that the enzymatic hydrolysis yield of poplar sawdust xylan was improved by adding Xln-DT, and a xylose-rich hydrolysate could be obtained at high purity, with the xylose yield of 89.9%. The enzymatic hydrolysis yield was higher (32.2%) by using MxynB-8 and Xln-DT together. This study provides a deep understanding of double-enzyme synergetic enzymolysis of wood polysaccharides to valuable products.

Sign in / Sign up

Export Citation Format

Share Document