scholarly journals Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 191
Author(s):  
Hyun Tae Kim ◽  
Enjian Jin ◽  
Min-Ho Lee
Keyword(s):  
Human Serum ◽  
Limit Of Detection ◽  
Serum Cortisol ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Nanoparticle Probes ◽  
Luminescent Signal ◽  
Microplate Reader ◽  
Sensitive Determination

In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R2 = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R2 = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ.

Prussian blue nanoparticles based lateral flow assay for high sensitive determination of clenbuterol

2018 ◽  
Vol 275 ◽  
pp. 223-229 ◽  
Author(s):  
Bingxin Zhao ◽  
Qiong Huang ◽  
Leina Dou ◽  
Tong Bu ◽  
Kai Chen ◽  
...  
Keyword(s):  
Prussian Blue ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Prussian Blue Nanoparticles ◽  
Sensitive Determination

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM).The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated at a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM). The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

Sensitive detection of microRNA-21 in cancer cells and human serum with Au@Si nanocomposite and lateral flow assay

2021 ◽  
Vol 1147 ◽  
pp. 56-63
Author(s):  
Tao Dong ◽  
Ran Yin ◽  
Qingcai Yu ◽  
Wanwei Qiu ◽  
Kun Li ◽  
...  
Keyword(s):  
Human Serum ◽  
Cancer Cells ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Sensitive Detection

Detection of peroxyacetyl nitrate in air using chemiluminescence aerosol detector

2014 ◽  
Vol 68 (11) ◽  
Author(s):  
Pavel Mikuška ◽  
Lukáš Bružeňák ◽  
Zbyněk Večeřa
Keyword(s):  
Alkaline Solution ◽  
Limit Of Detection ◽  
Packed Column ◽  
Direct Reaction ◽  
Peroxyacetyl Nitrate ◽  
Brij 35 ◽  
Alternative Approach ◽  
Sensitive Determination ◽  
Triton X

AbstractA method for the rapid and sensitive determination of peroxyacetyl nitrate (PAN) in air based on a chemiluminescence reaction with an alkaline solution of luminol in the chemiluminescence aerosol detector is described. The PAN is chromatographically separated from nitrogen dioxide and ozone in a packed column filled with 5 % OV-1 on Chromosorb 30/60 and the eluted PAN is detected via the direct reaction with the luminol solution consisting of 0.002 mol L−1 luminol, 1 vol. % Brij-35 and 0.1 mol L−1 KOH. The limit of detection is 14.9 ng m−3 (3 ppt) of PAN. Alternatively, the PAN after separation is thermally converted to NO2 which is detected by the chemiluminescence reaction with a solution consisting of 0.002 mol L−1 luminol, 0.5 mol L−1 KOH, 0.2 mol L−1 Na2SO3, 0.1 mol L−1 KI, 0.05 mol L−1 EDTA and 0.5 vol. % triton X-100. The alternative approach affords the simultaneous determination of PAN and NO2. The limit of detection is 50 ppt of PAN and 50 ppt of NO2. The time resolution is 3 min. The method was applied to the measurement of ambient peroxyacetyl nitrate in air.

scholarly journals Determination of uric acid in human serum by an enzymatic method using N-methyl-N-(4-aminophenyl)-3-methoxyaniline reagent

2003 ◽  
Vol 68 (8-9) ◽  
pp. 691-698 ◽  
Author(s):  
Milena Jelikic-Stankov ◽  
Predrag Djurdjevic ◽  
Dejan Stankov
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Limit Of Detection ◽  
Ascorbate Oxidase ◽  
Acid Oxidation ◽  
Uric Acid Concentration ◽  
Enzymatic Method ◽  
Limit Of Quantification ◽  
Relative Standard

In this work a new enzymatic method for the determination of uric acid in human serum has been developed. The method is based on the oxidative coupling reaction between the N-methyl-N-(4-aminophenyl)-3-methoxyaniline (NCP) reagent and the hydrogen ? donor reagent N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (TOOS), in the system involving three enzymes: uricase, peroxidase and ascorbate oxidase. Using this method uric acid could be determined in concentrations up to 1.428 mmol/L, with a relative standard deviation of up to 1.8 %. The effect of the medium pH and the NCP concentration on the linearity of the chromogen absorbance versus the uric acid concentration curve was investigated. The influence of the uricase activity on the maximum rate of uric acid oxidation was also examined. The use of the NCP reagent demonstrated a more precise and more sensitive determination of the uric acid compared to the determination with 4-aminoantipyrine (4-AA) as the coupling regent. The sensitivity of the method determined from the calibration curve was 0.71 absorbance units per mmol/L of uric acid; the limit of detection was LOD = 0.0035 mmol/L and the limit of quantification was LOQ = 0.015 mmol/L of uric acid.

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