scholarly journals Electrocatalysis by Heme Enzymes—Applications in Biosensing

Catalysts ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 218
Author(s):  
Lidia Zuccarello ◽  
Catarina Barbosa ◽  
Smilja Todorovic ◽  
Célia M. Silveira
Keyword(s):  
Cytochrome C ◽  
Heme Proteins ◽  
Rational Design ◽  
Structural Information ◽  
Heme Iron ◽  
Biological Processes ◽  
Heme Enzymes ◽  
Long Term Stability ◽  
And Storage

Heme proteins take part in a number of fundamental biological processes, including oxygen transport and storage, electron transfer, catalysis and signal transduction. The redox chemistry of the heme iron and the biochemical diversity of heme proteins have led to the development of a plethora of biotechnological applications. This work focuses on biosensing devices based on heme proteins, in which they are electronically coupled to an electrode and their activity is determined through the measurement of catalytic currents in the presence of substrate, i.e., the target analyte of the biosensor. After an overview of the main concepts of amperometric biosensors, we address transduction schemes, protein immobilization strategies, and the performance of devices that explore reactions of heme biocatalysts, including peroxidase, cytochrome P450, catalase, nitrite reductase, cytochrome c oxidase, cytochrome c and derived microperoxidases, hemoglobin, and myoglobin. We further discuss how structural information about immobilized heme proteins can lead to rational design of biosensing devices, ensuring insights into their efficiency and long-term stability.

scholarly journals Rational design of MgF2 catalysts with long-term stability for the dehydrofluorination of 1,1-difluoroethane (HFC-152a)

RSC Advances ◽  
2019 ◽  
Vol 9 (41) ◽  
pp. 23744-23751 ◽  
Author(s):  
Haodong Tang ◽  
Mingming Dang ◽  
Yuzhen Li ◽  
Lichun Li ◽  
Wenfeng Han ◽  
...  
Keyword(s):  
Rational Design ◽  
Sol Gel ◽  
Hard Template ◽  
Term Stability ◽  
Long Term Stability ◽  
Improved Stability ◽  
Hfc 152A ◽  
Template Methods

In this study, sol–gel, precipitation and hard-template methods were applied to synthesize MgF2 catalysts with improved stability towards dehydrofluorination of hydrofluorocarbons and MgF2-T catalysts demonstrated superior long-term stability.

Minicomputer as a circuit-design tool

SIMULATION ◽  
1972 ◽  
Vol 18 (2) ◽  
pp. 47-56 ◽  
Author(s):  
Zvonko Fazarinc
Keyword(s):  
Circuit Design ◽  
Computer Aided Design ◽  
Design Tool ◽  
Nonlinear Networks ◽  
Long Term Stability ◽  
Processing Cost ◽  
Aided Design ◽  
And Storage ◽  
High Processing

Lack of interactive capabilities and high processing cost are two main areas of complaints directed against the existing implementations of computer- aided-design packages. The minicomputer promises to remove these serious deficiencies despite its speed and storage limitations. The economics and techniques of interactive minicomputer applications are explored and an integration formula suitable for minicomputer implementation is devised. Several nonlinear networks simulated on a minicomputer are used to demonstrate the long-term stability of the formula.

Preparation of Glucose Sensor Using Polydimethylsiloxane / Polypyrrole Complex

2003 ◽  
Vol 17 (08n09) ◽  
pp. 1217-1222 ◽  
Author(s):  
Mikito Yasuzawa ◽  
Shigeru Inoue ◽  
Shinji Imai
Keyword(s):  
Glucose Sensor ◽  
Platelet Rich Plasma ◽  
Glucose Sensors ◽  
Wire Electrode ◽  
Term Stability ◽  
Long Term Stability ◽  
Response Current ◽  
And Storage ◽  
Storage Temperatures

New glucose oxidase (GOD) immobilized glucose sensors were prepared by the electropolymerization of 1-(6-D-gluconamidohexyl) pyrrole (GHP) on the platinum wire electrode precoated with the mixture solution of pyrrole derivative GHP, polydimethylsiloxane (PDS) and Nafion. The addition of Nafion into the precoating mixture solution was essential to obtain suitable sensor sensitivity. However, the sensitivity was about the half of that of the electrode without PDS precoating. Although, the introduction of Nafion was effective to improve the long-term stability of the enzyme-immobilized electrode, the electrode prepared using Nafion, PDS and GHP performed excellent long-term stability even at the measurement and storage temperatures of 40°C. Relatively constant response current was obtained over 30 days under the condition of 40°C and over 9 months measured at 25°C. Moreover, the GOD-immobilized GHP polymer film prepared on the electrode precoated with GHP, PDS and Nafion solution, was found to have excellent hemocompatibility from the result of platelet rich plasma contacting test.

scholarly journals Impact of Fluorine atoms on triphenylamine-based dopant free hole selective layer for perovskite solar cells

2021 ◽  
Author(s):  
Abolfazl Ghaderian ◽  
Meenakshi Pegu ◽  
Naveen Harindu Hemasiri ◽  
Peng Huang ◽  
Shahzada Ahmad ◽  
...  
Keyword(s):  
Solar Cells ◽  
Rational Design ◽  
Perovskite Solar Cells ◽  
Cost Effective ◽  
Free Hole ◽  
Selective Layer ◽  
Term Stability ◽  
Long Term Stability ◽  
Hole Transporting

For industrial endeavors, perovskite solar cells (PSCs) demand long-term stability and a cost-effective hole-transporting layer (HTL). The PSC stability can be substantially improved by the rational design of dopant-free HTL...

scholarly journals Considerations for Stability of Environmental Samples in Storage for Long-Term Studies

2021 ◽  
Author(s):  
Susan Marie Viet ◽  
Maire S.A. Heikkinen ◽  
Michael Dellarco
Keyword(s):  
Environmental Samples ◽  
Storage Period ◽  
Storage Conditions ◽  
Research Community ◽  
Environmental Research ◽  
Extensive Literature ◽  
Long Term Stability ◽  
Sample Stability ◽  
And Storage

It is often advantageous to store collected environmental samples for future retrospective analyses. However, information about sample stability is necessary to determine if there will be analyte loss or gain or degradation under the specified storage conditions and storage period. Failure to evaluate stability could result in inaccurate results and biased exposure assessments. As part of the National Children’s Study pilot, we considered which types of environmental samples could be stored for extended periods of time. We conducted an extensive literature review and considered the conduct of long-term stability studies for environmental samples. We present our findings and experience below as guidance for consideration by the environmental research community.

scholarly journals Controlling colloidal stability of silica nanoparticles during bioconjugation reactions with proteins and improving their longer-term stability, handling and storage

2015 ◽  
Vol 3 (10) ◽  
pp. 2043-2055 ◽  
Author(s):  
C. J. Moore ◽  
H. Montón ◽  
R. O'Kennedy ◽  
D. E. Williams ◽  
C. Nogués ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Colloidal Stability ◽  
Improved Method ◽  
Term Stability ◽  
Long Term Stability ◽  
And Storage

Robust protocols for antibody-nanoparticle (Ab-NP) conjugation, and improved method for long-term stability and storage of Ab-NPs using cryoprotectants.

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