scholarly journals Small-RNA Sequencing Reveals Altered Skeletal Muscle microRNAs and snoRNAs Signatures in Weanling Male Offspring from Mouse Dams Fed a Low Protein Diet during Lactation

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1166
Author(s):  
Ioannis Kanakis ◽  
Moussira Alameddine ◽  
Leighton Folkes ◽  
Simon Moxon ◽  
Ioanna Myrtziou ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rna Sequencing ◽  
Small Rna ◽  
Molecular Mechanisms ◽  
Male Offspring ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Histological Evaluation ◽  
Small Rna Sequencing ◽  
Low Protein

Maternal diet during gestation and lactation affects the development of skeletal muscles in offspring and determines muscle health in later life. In this paper, we describe the association between maternal low protein diet-induced changes in offspring skeletal muscle and the differential expression (DE) of small non-coding RNAs (sncRNAs). We used a mouse model of maternal protein restriction, where dams were fed either a normal (N, 20%) or a low protein (L, 8%) diet during gestation and newborns were cross-fostered to N or L lactating dams, resulting in the generation of NN, NL and LN offspring groups. Total body and tibialis anterior (TA) weights were decreased in weanling NL male offspring but were not different in the LN group, as compared to NN. However, histological evaluation of TA muscle revealed reduced muscle fibre size in both groups at weaning. Small RNA-sequencing demonstrated DE of multiple miRs, snoRNAs and snRNAs. Bioinformatic analyses of miRs-15a, -34a, -122 and -199a, in combination with known myomiRs, confirmed their implication in key muscle-specific biological processes. This is the first comprehensive report for the DE of sncRNAs in nutrition-associated programming of skeletal muscle development, highlighting the need for further research to unravel the detailed molecular mechanisms.

scholarly journals Small-RNA sequencing reveals altered skeletal muscle microRNAs and snoRNAs signatures in weanling male offspring from mouse dams fed a low protein diet during lactation

2021 ◽  
Author(s):  
Ioannis Kanakis ◽  
Moussira Alameddine ◽  
Leighton Folkes ◽  
Simon Moxon ◽  
Ioanna Myrtziou ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Small Rna ◽  
Molecular Mechanisms ◽  
Male Offspring ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Histological Evaluation ◽  
Small Rna Sequencing ◽  
Low Protein ◽  
The Impact

ABSTRACTNutrition plays a key role in pre- and postnatal growth of the musculoskeletal system. Maternal diet during gestation and lactation affects the development of skeletal muscles in the offspring and determines muscle health in later life, however, the molecular mechanisms that govern these effects are largely unknown. In this study, we aim to describe the association between maternal low protein diet-induced changes in offspring skeletal muscle and the differential expression (DE) of small non-coding RNAs (sncRNAs). We used a mouse model of maternal protein restriction to characterise the impact of early-life undernutrition on skeletal muscle morphology in male offspring at weaning. Mouse dams were fed either a normal (N, 20%) or a low protein (L, 8%) diet during gestation and newborn pups were cross-fostered to N or L lactating dams, resulting in the generation of NN, NL and LN offspring groups. Total body and tibialis anterior (TA) weights were decreased in NL males but not different in the LN group, as compared to NN, although neonates from low protein fed dams were smaller at birth than those born to dams fed a normal protein. However, histological evaluation of TA muscle revealed reduced muscle fibre size in both groups at the end of lactation. Small RNA-seq analysis demonstrated DE of multiple classes of sncRNAs, including miRs, snoRNAs and snRNAs. Bioinformatic analyses of miRs-15a, −34a, −122 and −199a, in combination with known myomiRs, confirmed their implication in key muscle-specific biological processes and cellular functions and suggest a promising set of miRs in muscle physiology studies. To our knowledge, this is the first comprehensive report for the DE of sncRNAs in nutrition-associated programming of skeletal muscle development, highlighting the need for further research.

scholarly journals S12.43 Gene expression profiling of liver and skeletal muscle in newborn mice exposed to an in utero low protein diet with or without taurine

2008 ◽  
Vol 1777 ◽  
pp. S86
Author(s):  
Ole H. Mortensen ◽  
Hanne L. Olsen ◽  
Lis Frandsen ◽  
Peter E. Nielsen ◽  
Finn C. Nielsen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
In Utero ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Newborn Mice ◽  
Low Protein

Dietaryl-Lysine Suppresses Autophagic Proteolysis and Stimulates Akt/mTOR Signaling in the Skeletal Muscle of Rats Fed a Low-Protein Diet

2015 ◽  
Vol 63 (37) ◽  
pp. 8192-8198 ◽  
Author(s):  
Tomonori Sato ◽  
Yoshiaki Ito ◽  
Takashi Nagasawa
Keyword(s):  
Skeletal Muscle ◽  
Mtor Signaling ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Low Protein

scholarly journals Effect of a low-protein diet supplemented with keto-acids on autophagy and inflammation in 5/6 nephrectomized rats

2015 ◽  
Vol 35 (5) ◽  
Author(s):  
Yue-yue Zhang ◽  
Juan Huang ◽  
Man Yang ◽  
Li-jie Gu ◽  
Jia-yao Ji ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Muscle Mass ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Low Protein ◽  
Keto Acids

The present study demonstrated that autophagy/mitophagy was increased and inflammation was aggravated in skeletal muscle in chronic kidney disease (CKD) rats. A low-protein diet (LPD) supplemented with ketoacids (KA) improved the loss in muscle mass and blocked the activation of autophagy/mitophagy and inflammation in those rats.

scholarly journals Paternal low protein diet affects adult offspring cardiovascular and metabolic function in mice

2014 ◽  
Vol 306 (10) ◽  
pp. H1444-H1452 ◽  
Author(s):  
Adam J. Watkins ◽  
Kevin D. Sinclair
Keyword(s):  
Body Weight ◽  
Vascular Dysfunction ◽  
Weight Ratio ◽  
Male Offspring ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Adult Offspring ◽  
Female Ratio ◽  
Low Protein ◽  
The Impact

Although the association between maternal periconceptional diet and adult offspring health is well characterised, our understanding of the impact of paternal nutrition at the time of conception on offspring phenotype remains poorly defined. Therefore, we determined the effect of a paternal preconception low protein diet (LPD) on adult offspring cardiovascular and metabolic health in mice. Male C57BL/6 mice were fed either normal protein diet (NPD; 18% casein) or LPD (9% casein) for 7 wk before mating. At birth, a reduced male-to-female ratio ( P = 0.03) and increased male offspring weight ( P = 0.009) were observed in litters from LPD compared with NPD stud males with no differences in mean litter size. LPD offspring were heavier than NPD offspring at 2 and 3 wk of age ( P < 0.02). However, no subsequent differences in body weight were observed. Adult male offspring derived from LPD studs developed relative hypotension (decreased by 9.2 mmHg) and elevated heart rate ( P < 0.05), whereas both male and female offspring displayed vascular dysfunction and impaired glucose tolerance relative to NPD offspring. At cull (24 wk), LPD males had elevated adiposity ( P = 0.04), reduced heart-to-body weight ratio ( P = 0.04), and elevated circulating TNF-α levels ( P = 0.015) compared with NPD males. Transcript expression in offspring heart and liver tissue was reduced for genes involved in calcium signaling ( Adcy, Plcb, Prkcb) and metabolism ( Fto) in LPD offspring ( P < 0.03). These novel data reveal the impact of suboptimal paternal nutrition on adult offspring cardiovascular and metabolic homeostasis, and provide some insight into the underlying regulatory mechanisms.

scholarly journals Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ying Liang ◽  
Kunhua Wei ◽  
Fan Wei ◽  
Shuangshuang Qin ◽  
Chuanhua Deng ◽  
...  
Keyword(s):  
Drought Stress ◽  
Rna Sequencing ◽  
Small Rna ◽  
Stress Responses ◽  
Molecular Mechanisms ◽  
Differentially Expressed ◽  
Small Rna Sequencing ◽  
Severe Drought ◽  
A Genome ◽  
Sophora Tonkinensis

Abstract Background Sophora tonkinensis Gagnep is a traditional Chinese medical plant that is mainly cultivated in southern China. Drought stress is one of the major abiotic stresses that negatively impacts S. tonkinensis growth. However, the molecular mechanisms governing the responses to drought stress in S. tonkinensis at the transcriptional and posttranscriptional levels are not well understood. Results To identify genes and miRNAs involved in drought stress responses in S. tonkinensis, both mRNA and small RNA sequencing was performed in root samples under control, mild drought, and severe drought conditions. mRNA sequencing revealed 66,476 unigenes, and the differentially expressed unigenes (DEGs) were associated with several key pathways, including phenylpropanoid biosynthesis, sugar metabolism, and quinolizidine alkaloid biosynthesis pathways. A total of 10 and 30 transcription factors (TFs) were identified among the DEGs under mild and severe drought stress, respectively. Moreover, small RNA sequencing revealed a total of 368 miRNAs, including 255 known miRNAs and 113 novel miRNAs. The differentially expressed miRNAs and their target genes were involved in the regulation of plant hormone signal transduction, the spliceosome, and ribosomes. Analysis of the regulatory network involved in the response to drought stress revealed 37 differentially expressed miRNA-mRNA pairs. Conclusion This is the first study to simultaneously profile the expression patterns of mRNAs and miRNAs on a genome-wide scale to elucidate the molecular mechanisms of the drought stress responses of S. tonkinensis. Our results suggest that S. tonkinensis implements diverse mechanisms to modulate its responses to drought stress.

scholarly journals Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma—piR-9491 and piR-12488 Reduce Tumor Cell Colonies In Vitro

2021 ◽  
Vol 11 ◽  
Author(s):  
Michael Bartos ◽  
Frantisek Siegl ◽  
Alena Kopkova ◽  
Lenka Radova ◽  
Jan Oppelt ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Small Rna ◽  
Genome Stability ◽  
Molecular Mechanisms ◽  
Prognostic Biomarker ◽  
Independent Set ◽  
Small Rna Sequencing ◽  
Sequencing Analysis ◽  
Tumor Patients

Glioblastoma (GBM) is the most frequently occurring primary malignant brain tumor of astrocytic origin. To change poor prognosis, it is necessary to deeply understand the molecular mechanisms of gliomagenesis and identify new potential biomarkers and therapeutic targets. PIWI-interacting RNAs (piRNAs) help in maintaining genome stability, and their deregulation has already been observed in many tumors. Recent studies suggest that these molecules could also play an important role in the glioma biology. To determine GBM-associated piRNAs, we performed small RNA sequencing analysis in the discovery set of 19 GBM and 11 non-tumor brain samples followed by TaqMan qRT-PCR analyses in the independent set of 77 GBM and 23 non-tumor patients. Obtained data were subsequently bioinformatically analyzed. Small RNA sequencing revealed 58 significantly deregulated piRNA molecules in GBM samples in comparison with non-tumor brain tissues. Deregulation of piR-1849, piR-9491, piR-12487, and piR-12488 was successfully confirmed in the independent groups of patients and controls (all p &lt; 0.0001), and piR-9491 and piR-12488 reduced GBM cells’ ability to form colonies in vitro. In addition, piR-23231 was significantly associated with the overall survival of the GBM patients treated with Stupp regimen (p = 0.007). Our results suggest that piRNAs could be a novel promising diagnostic and prognostic biomarker in GBM potentially playing important roles in gliomagenesis.

scholarly journals RNA-Sequencing Analysis of Paternal Low-Protein Diet-Induced Gene Expression Change in Mouse Offspring Adipocytes

2019 ◽  
Vol 9 (7) ◽  
pp. 2161-2170 ◽  
Author(s):  
Nhung Hong Ly ◽  
Toshio Maekawa ◽  
Keisuke Yoshida ◽  
Yang Liu ◽  
Masafumi Muratani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Sequencing ◽  
Gene Expression Change ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Sequencing Analysis ◽  
Expression Change ◽  
Low Protein

A translation repressor, 4E-BP1, regulates the triglyceride level in rat liver during protein deprivation

2020 ◽  
Vol 318 (5) ◽  
pp. E636-E645
Author(s):  
Yuka Toyoshima ◽  
Fumiaki Yoshizawa ◽  
Reiko Tokita ◽  
Yusuke Taguchi ◽  
Shin-Ichiro Takahashi ◽  
...  
Keyword(s):  
Rat Liver ◽  
Molecular Mechanisms ◽  
Mrna Level ◽  
Translation Initiation Factor ◽  
Protein Diet ◽  
Initiation Factor ◽  
Low Protein Diet ◽  
Acid Oxidation ◽  
Protein Deprivation ◽  
Low Protein

Protein deprivation has been shown to induce fatty liver in humans and animals, but the molecular mechanisms underlying such induction are largely unknown. Our previous studies have shown that a low-protein diet increases eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) protein and triglyceride (TG) levels in rat liver. 4E-BP1 is known to repress translation by binding to eIF4E. There is also evidence indicating that 4E-BP1 regulates lipid metabolism. Here, we examined the role of 4E-BP1 on TG accumulation in the livers of rats under protein deprivation. The low-protein diet rapidly increased the hepatic 4E-BP1 mRNA level within 1 day, followed by the induction of hepatic TG accumulation. The knockdown of hepatic 4E-BP1 attenuated the TG accumulation in rat liver induced by the low-protein diet. 4E-BP1 knockdown also increased the protein level of carnitine palmitoyltransferase 1A (CPT1A), a regulator of fatty acid oxidation, in the liver of rats fed a low-protein diet. These results indicate that a low-protein diet increases the amount of 4E-BP1, leading to TG accumulation in rat liver. We thus conclude that 4E-BP1 plays an important role in inducing hepatic steatosis under protein deprivation.

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