Regional Variation in Dengue Virus Serotypes in Sri Lanka and Its Clinical and Epidemiological Relevance

Dengue is a significant health concern in Sri Lanka, but diagnosis of the infecting dengue virus (DENV) serotype has hitherto been largely restricted to the Colombo district in the western province. Salinity tolerant Aedes vectors are present in the island’s northern Jaffna peninsula, which is undergoing rapid groundwater salinization. Virus serotypes were determined by RT-qPCR in 107 and 112 patients diagnosed by NS1 antigen positivity from the Jaffna district in 2018 and 2019, respectively, and related to clinical characteristics. DENV1 and DENV2 were the most common serotypes in both years. Infections with multiple serotypes were not detected. DENV1 was significantly more prevalent in 2019 than 2018, while DENV3 was significantly more prevalent in 2018 than 2019 among the Jaffna patients. Limited genomic sequencing identified DENV1 genotype-I and DENV3 genotype-I in Jaffna patients in 2018. Dengue was more prevalent in working age persons and males among the serotyped Jaffna patients. DENV1 and DENV2 were the predominant serotypes in 2019 in the Colombo district. However, DENV1 and DENV3 were significantly more prevalent in Colombo compared with Jaffna in 2019. The differences in the prevalence of DENV1 and DENV3 between the Jaffna and Colombo districts in 2019 have implications for dengue epidemiology and vaccination. Salinity-tolerant Aedes vector strains, widespread in the Jaffna peninsula, may have contributed to differences in serotype prevalence compared with the Colombo district in 2019. Significant associations were not identified between virus serotypes and clinical characteristics among Jaffna patients.

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Asian genotypes of dengue virus 4 in Brazil

Epidemiology and Infection ◽

10.1017/s0950268815000229 ◽

2015 ◽

Vol 143 (14) ◽

pp. 3114-3117 ◽

Cited By ~ 4

Author(s):

A. C. O. PINHO ◽

S. I. SARDI ◽

F. L. PAULA ◽

I. B. PEIXOTO ◽

C. J. BRANDÃO ◽

...

Keyword(s):

Public Health ◽

Phylogenetic Analysis ◽

Dengue Virus ◽

Health Problem ◽

Social Impact ◽

Public Health Problem ◽

Genotype I ◽

Asian Origin ◽

Northern Brazil ◽

Dengue Virus Serotypes

SUMMARYDengue virus, commonly transmitted by mosquitoes, causes a human disease of significant social impact and presents a serious public health problem in Brazil. This report describes the unusual emergence of DENV-4 in northern Brazil after a nearly 30-year-long absence. DENV-4 genotype I is of Asian origin and was identified in the serum of patients receiving treatment at a hospital serving the Salvador area (Brazilian state of Bahia). The identification of dengue virus serotypes through molecular and phylogenetic analysis is essential for predicting disease severity or fatal illness, principally in endemic countries such as Brazil.

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KETERKAITAN ANTIGEN NS1 INFEKSI VIRUS DENGUE DENGAN SEROTIPE VIRUS DENGUE

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v18i2.1005 ◽

2018 ◽

Vol 18 (2) ◽

pp. 87

Author(s):

Roudhotul Ismaillya Noor ◽

Aryati Aryati ◽

Puspa Wardhani

Keyword(s):

Dengue Virus ◽

Virus Isolation ◽

Serological Test ◽

Dengue Virus Infection ◽

Serological Tests ◽

Ns1 Antigen ◽

Dengue Virus Serotypes ◽

Polymerase Chain ◽

Test Result ◽

Better Than

Dengue virus infection (DVI) currently is detected by using dengue virus NS1 antigen (NS1 Ag). The sensitivity of NS1 Ag is 27.8%–93.4%,but recent study of Kumarasamy the sensitivity of NS1 Ag is better than the virus isolation and polymerase chain reaction (PCR). This studyis focussed on the evaluation of the validity of Panbio Dengue Early Rapid for the diagnosis of DVI and the NS1 Ag sensitivity associated withdengue virus serotypes. The sera was obtained from 65 DVI patients which diagnosed by the clinicians. The resulted diagnosis was foundby serology tests (positive IgM/IgG antidengue/NS1 Ag ELISA) and 1997 WHO criteria as the gold standard, and which also found 35 nonDVI patients (typhoid fever, HAV, malaria, UTI, tuberculosis and bronchopneumonia). The samples were examined by Panbio Dengue EarlyRapid. PCR was performed on each positive serological test result to determine the dengue virus serotypes. The sensitivity and specificity ofPanbio Dengue Early Rapid was 49.2% and 100%. The PCR results of 65 sera showed positive PCR in 49.2% (positive NS1 Ag was 62.5%).Meanwhile, and negative PCR in 50.8% (positive NS1 Ag was 36.4%). The predominance of serotypes (positive NS1 Ag) were DEN-3 (37.5%),DEN-4 (28.1%), DEN-1 (21.9%) and DEN-2 (12.5%). The Panbio Dengue Early Rapid can be used as early detection of DVI, although itshould be used in conjunction with other dengue serological tests as well. Unfortunately there is still not enough evidence about the NS1 Agsensitivity associated with the dengue virus serotypes.

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Emergence of genotype I of dengue virus serotype 3 during severe dengue epidemic in Sri Lanka, 2017

Japanese Journal of Infectious Diseases ◽

10.7883/yoken.jjid.2020.854 ◽

2021 ◽

Author(s):

Mya Myat Ngwe Tun ◽

Rohitha Muthugala ◽

Lakmali Rajamanthri ◽

Takeshi Nabeshima ◽

Corazon C. Buerano ◽

...

Keyword(s):

Sri Lanka ◽

Dengue Virus ◽

Severe Dengue ◽

Genotype I ◽

Virus Serotype ◽

Dengue Epidemic

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Co-circulation of all the four dengue virus serotypes and detection of a novel clade of DENV-4 (genotype I) virus in Pune, India during 2016 season

PLoS ONE ◽

10.1371/journal.pone.0192672 ◽

2018 ◽

Vol 13 (2) ◽

pp. e0192672 ◽

Cited By ~ 27

Author(s):

Shubham Shrivastava ◽

Divya Tiraki ◽

Arundhati Diwan ◽

Sanjay K. Lalwani ◽

Meera Modak ◽

...

Keyword(s):

Dengue Virus ◽

Genotype I ◽

Dengue Virus Serotypes

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The citrus flavanone naringenin impairs dengue virus replication in human cells

Scientific Reports ◽

10.1038/srep41864 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 35

Author(s):

Sandra Frabasile ◽

Andrea Cristine Koishi ◽

Diogo Kuczera ◽

Guilherme Ferreira Silveira ◽

Waldiceu Aparecido Verri ◽

...

Keyword(s):

Dengue Virus ◽

Virus Replication ◽

Fruits And Vegetables ◽

Suitable Candidate ◽

Tropical Regions ◽

Virus Activity ◽

Dengue Virus Serotypes ◽

Medicinal Properties ◽

Virus Exposure ◽

Significant Health

Abstract Dengue is one of the most significant health problems in tropical and sub-tropical regions throughout the world. Nearly 390 million cases are reported each year. Although a vaccine was recently approved in certain countries, an anti-dengue virus drug is still needed. Fruits and vegetables may be sources of compounds with medicinal properties, such as flavonoids. This study demonstrates the anti-dengue virus activity of the citrus flavanone naringenin, a class of flavonoid. Naringenin prevented infection with four dengue virus serotypes in Huh7.5 cells. Additionally, experiments employing subgenomic RepDV-1 and RepDV-3 replicon systems confirmed the ability of naringenin to inhibit dengue virus replication. Antiviral activity was observed even when naringenin was used to treat Huh7.5 cells 24 h after dengue virus exposure. Finally, naringenin anti-dengue virus activity was demonstrated in primary human monocytes infected with dengue virus sertoype-4, supporting the potential use of naringenin to control dengue virus replication. In conclusion, naringenin is a suitable candidate molecule for the development of specific dengue virus treatments.

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Clade Replacements in Dengue Virus Serotypes 1 and 3 Are Associated with Changing Serotype Prevalence

Journal of Virology ◽

10.1128/jvi.79.24.15123-15130.2005 ◽

2005 ◽

Vol 79 (24) ◽

pp. 15123-15130 ◽

Cited By ~ 125

Author(s):

Chunlin Zhang ◽

Mammen P. Mammen ◽

Piyawan Chinnawirotpisan ◽

Chonticha Klungthong ◽

Prinyada Rodpradit ◽

...

Keyword(s):

Dengue Virus ◽

Phylogenetic Trees ◽

Large Scale ◽

Temporal Structure ◽

Differential Susceptibility ◽

Epidemiological Data ◽

Genotype I ◽

Serotype 1 ◽

History Of ◽

Dengue Virus Serotypes

ABSTRACT The evolution of dengue virus (DENV) is characterized by phylogenetic trees that have a strong temporal structure punctuated by dramatic changes in clade frequency. To determine the cause of these large-scale phylogenetic patterns, we examined the evolutionary history of DENV serotype 1 (DENV-1) and DENV-3 in Thailand, where gene sequence and epidemiological data are relatively abundant over a 30-year period. We found evidence for the turnover of viral clades in both serotypes, most notably in DENV-1, where a major clade replacement event took place in genotype I during the mid-1990s. Further, when this clade replacement event was placed in the context of changes in serotype prevalence in Thailand, a striking pattern emerged; an increase in DENV-1 clade diversity was associated with an increase in the abundance of this serotype and a concomitant decrease in DENV-4 prevalence, while clade replacement was associated with a decline in DENV-1 prevalence and a rise of DENV-4. We postulate that intraserotypic genetic diversification proceeds at times of relative serotype abundance and that replacement events can result from differential susceptibility to cross-reactive immune responses.

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Optimization of Flow-Cytometry Based Assay for Measuring Neutralizing Antibody Responses against Each of the Four Dengue Virus Serotypes

Vaccines ◽

10.3390/vaccines9111339 ◽

2021 ◽

Vol 9 (11) ◽

pp. 1339

Author(s):

Pragati Sharma ◽

Kaustuv Nayak ◽

Elluri Seetharami Reddy ◽

Humaira Farooqi ◽

Kaja Murali-Krishna ◽

...

Keyword(s):

Public Health ◽

Flow Cytometry ◽

Dengue Virus ◽

Vaccine Development ◽

Neutralizing Antibody ◽

Neutralization Assay ◽

Antibody Responses ◽

Health Concern ◽

Dengue Virus Serotypes ◽

Focus Reduction

Dengue is an important public health problem worldwide, with India contributing nearly a third of global dengue disease burden. The measurement of neutralizing antibody responses is critical for understanding dengue pathophysiology, vaccine development and evaluation. Historically, dengue virus neutralization titers were measured using plaque reduction neutralization tests (PRNTs), which were later adapted to focus reduction neutralization tests (FRNTs). Given the slow and laborious nature of both these assays, there has been interest in adapting a high-throughput flow cytometry based neutralization assay. However, flow cytometry based assays typically underestimate neutralization titers, and in situations where the titers are low they can even fail to detect neutralization activity. In this study, by evaluating graded numbers of input Vero cell numbers and viral inoculum, we optimized the flow cytometry based neutralization assay in such a way that it is sensitive and scores titers that are in concordance with focus reduction neutralization tests for each of the four dengue virus serotypes (p < 0.0001). Given that dengue is a global public health concern, and several research groups are making efforts to understand its pathophysiology and accelerate vaccine development and evaluation both in India and worldwide, our findings have timely significance for facilitating these efforts.

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IDENTIFICATION OF DENGUE VIRUS SEROTYPES AT THE DR. SOETOMO HOSPITAL SURABAYA IN 2016 AND ITS CORRELATION WITH NS1 ANTIGEN DETECTION

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v23i2.1138 ◽

2018 ◽

Vol 23 (2) ◽

pp. 151

Author(s):

Jeine Stela Akualing ◽

Aryati Aryati ◽

Puspa Wardhani ◽

Usman Hadi

Keyword(s):

Polymerase Chain Reaction ◽

Dengue Virus ◽

Reverse Transcriptase ◽

Ribonucleic Acid ◽

Rna Virus ◽

Rt Pcr ◽

Chain Reaction ◽

Ns1 Antigen ◽

Dengue Virus Serotypes ◽

Polymerase Chain

Serotipe virus dengue yang beredar terus mengalami perubahan dan berbeda di setiap daerah. Pergeseran serotipe maupun genotipedi dalamnya, mempengaruhi terjadinya wabah dengue di berbagai negara. Perbedaan serotipe diduga bernasab dengan deteksi antigen(Ag) non-structural 1 (NS1), namun belum banyak penelitian yang mendukung hal tersebut. Penelitian potong lintang dikerjakan sejakFebruari-Agustus 2016 dan didapatkan 60 subjek infeksi virus dengue (IVD) dan 25 non-IVD. Ribonucleic acid (RNA) virus denguediperiksa di semua subjek menggunakan Simplexa Dengue Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)termasuk identifikasi serotipe virus dengue dan pemeriksaan NS1 menggunakan uji cepat NS1 Panbio. Perbedaan perbandingan variabelkategorikal dianalisis dengan uji Fisher Exact. Kenasaban antara serotipe dengan deteksi Ag NS1 dianalisis dengan Chi-Kuadrat. RNAvirus dengue terdeteksi di 43 dari 60 subjek IVD (71,7%). Serotipe terbanyak adalah DENV-3 (62,8%). Pergeseran dominasi serotipetelah terjadi di Surabaya, sebelumnya dari DENV-2 ke DENV-1 dan sekarang DENV-3, kemungkinan akibat mobilitas pejamu, transporvirus dan faktor geografis. Kepekaan uji cepat NS1 75% dan kekhasan 100%. Persentase deteksi NS1 antar serotipe berbeda bermakna(p=0,002). Deteksi NS1 lebih rendah pada DENV-1 dibandingkan DENV-2 (p=0,007) ataupun DENV-3 (p=0,003). Serotipe virusdengue bernasab dengan deteksi NS1 (p=0,005). Ciri serotipe maupun genotipe virus dengue kemungkinan mempengaruhi sekresiNS1. Telah terjadi pergeseran serotipe virus dengue di pasien IVD di Surabaya sehingga diperlukan surveillance berkesinambunganuntuk memperkirakan terjadinya wabah. Serotipe bernasab dengan deteksi NS1. Salah satu penyebab hasil negatif palsu NS1 adalahperbedaan serotipe.

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