scholarly journals SARS-CoV-2 Infection Prevalence in the Population of South-Eastern Poland

Diagnostics ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2115
Author(s):  
Ewa Stępień ◽  
Marcin Koleśnik ◽  
Katarzyna Mitura ◽  
Maria Malm ◽  
Bartłomiej Drop ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Demographic Data ◽  
Age Groups ◽  
Virus Detection ◽  
Healthcare Systems ◽  
Infection Prevalence ◽  
Rt Pcr ◽  
Viral Nucleic Acid ◽  
South Eastern ◽  
Pcr Method

COVID-19 outbreak began in Wuhan, China, and has spread to other continents, including Europe, placing pressure on healthcare systems. Poland is one of the European countries with the highest number of SARS-CoV-2 infections and COVID-19-related deaths. The aim of this study was to analyze the presence of SARS-CoV-2 in the population of south-eastern Poland. The correlation between viral infection and demographic data (gender, age, place of residence) and cancer was also investigated. A total of 44,801 samples were tested, of which 4862 cases were diagnosed with SARS-CoV-2 infections. A total of 14,970 samples were tested in cancer patients. The RT-PCR method was used to detect viral nucleic acid. In this study, significantly, the highest rate of virus detection was among people living in Lublin and the lowest among people living in a small town (p < 0.0001). Moreover, there was no significant relationship between sex and the frequency of virus detection. The highest number of SARS-CoV-2 infections was observed in the age groups 10–19, 20–29, 30–39, and 90+ (p = 0.0001). In cancer patients, the percentage of positive cases was significantly lower than in the rest (p = 0.0001).

A NEW REAL-TIME RT-PCR. METHOD FOR THE DETECTION OF PSA POSITIVE CELLS IN BLOOD OF PROSTATE CANCER PATIENTS

2006 ◽  
Vol 5 (14) ◽  
pp. 788
Author(s):  
G. Barbero ◽  
P. Destefanis ◽  
S. Procida ◽  
C. Fiori ◽  
C. Ceruti ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
Cancer Patients ◽  
Rt Pcr ◽  
Pcr Method

scholarly journals The Development of Pathogenicity of Avian Influenza Virus Isolated from Indonesia

2015 ◽  
Vol 18 (2) ◽  
pp. 133
Author(s):  
Michael Haryadi Wibowo ◽  
Agus Eko Srihanto ◽  
Khrisdiana Putri ◽  
Widya Asmara ◽  
Charles Rangga Tabbu
Keyword(s):  
Amino Acid ◽  
Cleavage Site ◽  
Virus Detection ◽  
Basic Amino Acid ◽  
Nucleotide Composition ◽  
Rt Pcr ◽  
Highly Pathogenic ◽  
Viral Stock ◽  
Fusion Site ◽  
Pcr Method

Highly pathogenic avian infl uenza outbreak in Indonesia has been reported in various poultry due toH5N1 subtype. The presence of multiple basic amino acids within the cleavage site of HA glycoprotein hasbeen identifi ed to be associated with the pathogenicity of avian infl uenza virus. The study was retrospectivestudy which was designed to characterize the cleavage site and fusion site region of haemagglutinin gene ofAIV isolated from various poultry in 2003 to 2013. Isolation, Identifi cation and propagation were carried outto collect viral stock. For virus detection, reverse transcriptase PCR (RT-PCR) method on H5 and N1 genefragment was performed. All of RT-PCR HA gene positive products were sequenced for further nucleotideanalysis and to determine the nucleotide composition at the targeted fragment. The results are all AIV isolateswere identifi ed as H5N1 subtype. The sequence analyses revealed some motives of basic amino acid motivethat were classifi ed as highly pathogenic avian infl uenza virus. Further analyses on fusion domain of all AIVisolated during the period 2003 to 2013 showed conserved amino acid. Keywords: avian infl uenza, haemagglutinin, cleavage site, basic amino acid, fusion site

Development of a highly sensitive nested RT-PCR method for Beet necrotic yellow vein virus detection

2001 ◽  
Vol 95 (1-2) ◽  
pp. 163-169 ◽  
Author(s):  
J Morris ◽  
G.R.G Clover ◽  
V.A Harju ◽  
S.A Hugo ◽  
C.M Henry
Keyword(s):  
Virus Detection ◽  
Yellow Vein ◽  
Rt Pcr ◽  
Highly Sensitive ◽  
Pcr Method

scholarly journals Clinical characteristics, outcomes and follow-up of COVID-19 infection in cancer patients

2020 ◽  
Author(s):  
Minghao Fang ◽  
Jianmin Ling ◽  
Yanqing Wu ◽  
Zhaohua Wang ◽  
Le Yang
Keyword(s):  
Cancer Survivors ◽  
Cancer Patients ◽  
Clinical Characteristics ◽  
Demographic Data ◽  
Signs And Symptoms ◽  
Rt Pcr ◽  
Cytokine Profiles ◽  
Tnf Α ◽  
Clinical Records

Abstract Purpose: The study is to describe the clinical characteristics, outcomes and follow-up of cancer patients with COVID-19. Methods: Clinical records, demographic data, signs and symptoms, laboratory results, cytokine profiles, chest CT scans, comorbidities, treatments, clinical outcomes, and RT-PCR of SARS-CoV-2 after discharge were retrospectively collected for fifty-six cancer patients with laboratory-confirmed COVID-19 pneumonia who were admitted to Tongji Hospital of Huazhong University of Science and Technology, Wuhan, China, from Feb 1 to Apr 1, 2020. Evidence of cytokine profiles were assessed by testing for the IL1β, IL2R, IL6, IL8, IL10, and TNF - α in the peripheral blood of severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) infected cancer patients. Results: Of 2143 patients with COVID-19, 56 cancer patients were included. The patients were divided into two groups, as cancer survivors, and cancer non-survivors. 12 (21%) patients with lymphopenia (0.5 [0.3-0.7]) had died during hospital stay. In non-survivors, IL2R, IL6, and IL10 were higher. 3(6.8%) cancer survivors with COVID-19 had positive RT-PCR test results again shortly after discharge. Conclusion: The mortality rate of COVID-19 among cancer patients are considerable. Cancer non-survivors are characterized by more severe lymphopenia and a higher levels of cytokines. Recovered cancer survivors still may be virus carriers.

scholarly journals Dengue Virus Detection by Serological and Molecular Method in Different Hospitals of Nepal

2013 ◽  
Vol 11 (2) ◽  
pp. 24-28
Author(s):  
Yogendra Shah ◽  
Govind Prasad Gupta ◽  
Kishor Pandey ◽  
Sher Bahadur Pun ◽  
Krishna Prasad Pant ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Age Groups ◽  
Virus Detection ◽  
Public Health Problem ◽  
Viral Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Rt Pcr ◽  
Serum Samples ◽  
Disease Hospital

Introduction: Dengue is an emerging mosquito-borne viral disease in the world and is the serious public health problem of Nepal. Methods: This study was designed to determine sero-epidemiology of dengue virus infection during the period (June-Nov) of 2010 among suspected patients with fever visiting Koshi Zonal Hospital (KZH), Biratnagar, Narayani sub-regional Hospital (NSH), Birgunj, Sukraraj Tropical and Infectious Disease Hospital (STIDH), Kathmandu and Dhading District Hospital (DDH), Dhadingbeshi. The sero-prevalence of anti-dengue IgM antibody was determined by enzyme linked immunosorbent assay (ELISA). Results: Among 271 serum samples tested, the anti-dengue IgM positivity was 14.4%. Sero-positivity in male was 10.7% of total and that in female was 3.7%.  Among different age groups, the highest positive cases 11.8% were from age group 15-50 years and found least among the age group above 50 years 0.4%. Out of 4 different hospitals, the highest positive positive cases from STIDH with 9.2% and the least positive cases were from DDH (0.4%). RT-PCR showed 4.7% positivity of 21 samples tested. Conclusions: Enzyme immunoassay and RT-PCR serological marker can be used to diagnose the acute patients of dengue during outbreaks.Medical Journal of Shree Birendra Hospital; July-December 2012/vol.11/Issue2/24-27 DOI: http://dx.doi.org/10.3126/mjsbh.v11i2.7905 

Detection of SARs CoV-2 during initial phase of pandemic from population of banas kantha district of Gujarat-India with special emphasis on pooled samples

2021 ◽  
Vol 7 (4) ◽  
pp. 295-299
Author(s):  
Hiren Patel ◽  
Parijat N Goswami
Keyword(s):  
Viral Load ◽  
Age Groups ◽  
High Viral Load ◽  
Rt Pcr ◽  
Viral Loads ◽  
Corona Virus ◽  
Asymptomatic Patients ◽  
Care Workers ◽  
Pcr Method ◽  
Pharynx Swab

Corona virus (SARs CoV-2) has caused immense effect on morbidity and mortality of the population globally. We undertook this study as we are a part of one of the network laboratories of ICMR to test the patient’s sample by RT PCR for the ORF 1 ab gene of corona virus. : For a period of one and half months (14 April to 31 May 2020) we tested the nasopharynx and oro-pharynx swab samples sent to us in VTM from the assigned districts of Gujarat. All the samples were subjected to RT PCR method by following standard methods. Total of 9.04%(256/2833) population was positive and 4.73%(139/2833) belonged to age groups 21-40 and 2.33% (66/2833) to 41-60yrs. Above the age of 60yrs there were only 0.95% (22/2833) cases which were positive. It was advantageous to pool the samples. Out of the number of pools prepared, we reported around 80% negative and rest were positive in pools. The study also included association of viral load and infectivity. We found that 12% of the asymptomatic people and 5.1% of symptomatic individuals had high viral load. : It is seen that the incidence of Novel corona virus -19 detection by RT PCR is a reliable method and the establishment of the Ct value and infectivity of the patient to the health care workers and relatives needs to be taken care of. Also, the study presents asymptomatic patients having high viral loads being highly infective.

Disseminated tumor cells in bone marrow of breast cancer patients–Comparison of immunocytochemistry and RT-PCR

2006 ◽  
Vol 66 (S 01) ◽  
Author(s):  
T Fehm ◽  
S Becker ◽  
MJ Banys ◽  
G Becker-Pergola ◽  
S Duerr-Stoerzer ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Disseminated Tumor Cells ◽  
Breast Cancer Patients ◽  
Rt Pcr
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