scholarly journals Inspection of the Microbiota in Endodontic Lesions

2019 ◽  
Vol 7 (2) ◽  
pp. 47 ◽  
Author(s):  
Mario Dioguardi ◽  
Giovanni Di Gioia ◽  
Gaetano Illuzzi ◽  
Claudia Arena ◽  
Vito Carlo Alberto Caponio ◽  
...  

The primary objective of endodontic therapy is to create a biologically acceptable environment within the root canal system that allows for the healing and maintenance of the health of the peri-radicular tissue. Bacteria are one of the main causes of pulp problems, and they have different methods of penetrating and invading the endodontic space such as through carious lesions, traumatic pulp exposures, and fractures. The types of bacteria found range from facultative anaerobes to aerobes, up to the most resistant species able to survive in nutrient-free environments; the bacterial species Enterococcus faecalis belongs to this last group. Enterococcus faecalis is considered one of the main causes of recurring apical periodontal lesions following endodontic treatment, with persistent lesions occurring even after re-treatment. The review presented in this paper was performed in accordance with the PRISMA protocol and covers articles from the related scientific literature that were sourced from PubMed, Scopus, and Google Scholar using the following terms as keywords: “endodontic treatment”, “endodontic bacteria”, “microbial endodontic”, and “endodontic failure”. Only the articles considered most relevant for the purposes of this paper were read in full and taken into consideration for the following review. The results show that Enterococcus faecalis, Actinomycetes, and Propionibacterium propionicum are the species most frequently involved in persistent radicular and extra-radicular infections.

2016 ◽  
Vol 27 (5) ◽  
pp. 578-583 ◽  
Author(s):  
Andressa Lamari Reis ◽  
◽  
Ricardo Reis Oliveira ◽  
Warley Luciano Fonseca Tavares ◽  
Thamyris Duque Silva Saldanha ◽  
...  

Abstract The aim of this study was to evaluate the gene expression of proinflammatory (RANKL, TNF-a and IFN-g) and regulatory (TGF-b and IL-10) cytokines as reaction to experimental infection by mono or bi-association of Fusobacterium nucleatum (ATCC 10953) and Enterococcus faecalis (ATCC 19433). F. nucleatum and E. faecalis, either in mono- or bi-association were inoculated into the root canal system (RCS) of Balb/c mice. Animals were sacrificed at 10 and 20 days after infection and periapical tissues surrounding the root were collected. The mRNA expression of the cytokines RANKL, TNF-a, IFN- g, TGF-b and IL-10 was assessed using real-time PCR. The Kruskal-Wallis test was used for statistical analysis. F. nucleatum mono-infection induced high expression of RANKL and TNF-a, while its modulation was due to IL-10. High expression of IFN-g at day 20 was up-regulated by E. faecalis and RANKL; TNF-a was up-regulated by an independent mechanism via IL-10 and TGF-b. Bi-association (F. nucleatum and E. faecalis) stimulated high expression of RANKL, TNF-a and IFN-g, which seemed to be modulated by TGF-b 20 days later. The gene expression of proinflammatory cytokines was more prominent in the earlier periods of the experimental periapical infection, which concomitantly decreased in the later period. This expression may be regulated by IL-10 and TGF-b in an infection-specific condition


2021 ◽  
Vol 67 (2) ◽  
pp. 101-106
Author(s):  
Sanziana Adina Scarlatescu ◽  
◽  
Irina Gheorghiu ◽  
George Nicola ◽  
Andrei O. Al Aloul ◽  
...  

The objectives of root canal treatment are to prevent the apical parodontitis, to heal the acute or cronic apical periodontits and to maintain the results, keeping the teeth on the arch in a functional stage. Thus, accuratelly elimination of soft and hard tissues (cleaning and shaping of the root canal system), disinfection and complete, homogeneous and tridimensional filling of the root canal system are very important to successful endodontic treatment. Modern concepts in endodontic treatment rely on technological progress and therefore many cases are successfully fulfilled, but the practitioner may be put in front of procedural erorrs both during and after endodontic treatment. Apical blockage, ledging, zipping, stripping, perforations in the floor of the pulp canal chamber or root canals perforations are the most common errors, but in many clinical situations they can be prevented and even surpassed once happened.


2017 ◽  
Vol 6 (2) ◽  
pp. 65-67
Author(s):  
Neha Saini ◽  
Abhinav C Singhal ◽  
Aditi S Jain ◽  
Jayesh Tiwari ◽  
Pratik Surana

ABSTRACT Introduction The main objective of root canal treatment is to disinfect the entire root canal system, and irrigation is an important step in reducing the bacterial load from the root canal system. However, irrigants currently used in the field of endodontics have their share of limitations, and the search for an ideal root canal irrigant continues. The use of herbal extracts as endodontic irrigants is today gaining popularity. Aims and objectives To evaluate and compare the antimicrobial potential of herbal extracts, such as neem (Azadirachta indica), triphala (Terminalia chebula), green tea (Camellia sinensis), and combination of neem and triphala as endodontic irrigants against Enterococcus faecalis. Materials and methods Freshly prepared extracts of neem, triphala, green tea, and 2% chlorhexidine were used to assess the antimicrobial efficiency against E. faecalis using the agar well diffusion test. Agar plates were incubated at 370°C for 24 hours in an incubator. The diameter of bacterial inhibition zones around each well was recorded to the nearest size in mm. Results Higher mean zone of inhibition was recorded in chlorhexidine followed by neem extract and combination of neem and triphala than triphala and green tea extract respectively. The lowest mean zone of inhibition was found in green tea extract. Conclusion From the present study, it can be concluded that neem leaf extract shows comparable zones of inhibition with that of chlorhexidine and combination of neem and triphala. How to cite this article Singhal AC, Jain AS, Tiwari J, Surana P, Saini N. Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study. Int J Experiment Dent Sci 2017;6(2):65-67.


2020 ◽  
Vol 18 (1) ◽  
pp. 27-30
Author(s):  
S. N. Razumova ◽  
A. S. Brago ◽  
Haydar Barakat ◽  
L. M. Khaskhanova ◽  
R. M. Bragunova

The success of endodontic treatment depends on many factors. The most important of them are the knowledge and manual skills of the dentist.Materials and methods. We analyzed the condition of teeth after endodontic treatment in 300 patients aged 20 to 70 years. Were studied 300 images of CBCT, the number of treated teeth and the number of cases of unsuccessful endodontic treatment was analyzed.Results. According to CBCT data, high-quality obturation of the root canal system was observed in 1335 (75%) endodontically treated teeth. The reasons for successful endodontic treatment are under-sealing of the root canal (16%), removal of material beyond the apex (5.6%), and missed root canals (3.4%).Conclusion. Clinical dental patient examination revealed that maxillary and mandibular molars most often undergo endodontic treatment.


2014 ◽  
Vol 18 (3) ◽  
Author(s):  
Matheus Albino Souza ◽  
Tiago Lange dos Santos ◽  
Alessandra Kuhn Dall’Magro ◽  
Fabiana Vieira Vier-Peliser ◽  
Luciana Ruschel dos Santos ◽  
...  

2020 ◽  
Vol 37 (1) ◽  
pp. 35-40
Author(s):  
Laura Sasser

Pulp debridement and disinfection in the pulp cavity is a critical step in achieving a successful root canal therapy. Microorganisms remaining in the root canal system after endodontic treatment are a main cause of root canal failure. The challenges faced in endodontic disinfection include the complex anatomy of the root canal system, the existence of a biofilm within the root canal, and the creation of a problematic smear layer during instrumentation of the canal. Historically, sodium hypochlorite and ethylenediaminetetraacetic acid have been utilized as irrigants and still remain as the most effective disinfectants due to their synergistic abilities to eradicate microorganisms, dissolve necrotic debris, and remove the smear layer and biofilm. This article addresses challenges in endodontic disinfection, objectives of endodontic irrigants, properties of an ideal irrigant, currently used irrigants, and irrigant delivery systems utilized in veterinary dentistry.


2021 ◽  
Vol 76 (1) ◽  
pp. 28-36
Author(s):  
Natasa Predin Djuric ◽  
Peet J Van der Vyver ◽  
Martin Vorster ◽  
Zunaid I Vally

The primary cause of a periapical inflammatory lesion is intra-radicular microbial infection. Prevention and elimination of apical periodontitis is achieved through successful endodontic treatment. Endodontic treatment is designed to maintain and restore the health of the periapical tissues and prevent periapical disease. It may be defined as the combination of mechanical instrumentation of the root canal system with bactericidal irrigation and obturation with an inert material. Technically, the goal of instrumentation and irrigation is to debride and entirely remove infected tissue debris from the root canal system and create a uniform conical shape that allows medicament delivery and adequate obturation. Microbiologically, the goal of instrumentation and irrigation is to eliminate micro-organisms, reduce their survival in the root canal system and neutralise any antigenic potential of the microbial components remaining in the canal.


2015 ◽  
Vol 16 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Dilek Helvacioglu-Yigit

ABSTRACT Aim C-shaped canal system is a seldom-found root canal anatomy which displays a challenge in all stages of endodontic treatment. According to the literature, this type of canal morphology is not a common finding in the mandibular first molar teeth. Background This case report presents endodontic management of a mandibular first molar with a C-shaped canal system. Case report Root canal system was cleaned and shaped by nickel-titanium (NiTi) rotary instruments combined with selfadjusting file (SAF). Obturation was performed using warm, vertical condensation combined with the injection of warm gutta-percha. Follow-up examination 12 months later showed that the tooth was asymptomatic. The radiological findings presented no signs of periapical pathology. Clinical significance The clinician must be aware of the occurence and complexity of C-shaped canals in mandibular first molar teeth to perform a successful root canal treatment. The supplementary use of SAF after application of rotary instruments in C-shaped root canals might be a promising approach in endodontic treatment of this type of canal morphology. How to cite this article Helvacioglu-Yigit D. Endodontic Management of C-shaped Root Canal System of Mandibular First Molar by using a modified Technique of Self-adjusting File System. J Contemp Dent Pract 2015;16(1):77-80.


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