scholarly journals Monitoring the Bioactive Compounds Status in Olea europaea According to Collecting Period and Drying Conditions

Energies ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 947 ◽  
Author(s):  
Amani Taamalli ◽  
Jesus Lozano Sánchez ◽  
Haifa Jebabli ◽  
Najla Trabelsi ◽  
Leila Abaza ◽  
...  
Keyword(s):  
High Performance ◽  
Reversed Phase ◽  
Olive Leaf ◽  
Leaf Extracts ◽  
Ionization Time ◽  
Drying Temperature ◽  
Flight Mass Spectrometry ◽  
Health Promoting ◽  
Drying Conditions ◽  
Phytochemical Profiles

Polyphenols and triterpenoids in olive have relevant importance both in the physiology of the plant and the nutritional and biological value of its products. Olive leaf extracts are of special interest for their numerous health-promoting properties. The present research is investigating the occurrence of phytochemicals in supercritical fluid extracts from leaves with regard to collection time and drying temperature. The phytochemical profiles of the olive leaf extracts were determined by reversed-phase high-performance liquid chromatography (HPLC) coupled to electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS) detection. The main extracted phytochemicals were phenols and terpenoids. A significant variation in the amounts of the different components was observed as a function of the different drying temperature and collecting time (p < 0.05). Among samples, the maximal contents of polyphenols and secoiridoid derivatives were found in the extracts from olive leaves collected in November and dried at 120 °C, whereas triterpenoids showed the highest content in fresh leaves collected in August.

scholarly journals A Quantitative Phytochemical Comparison of Olive Leaf Extracts on the Australian Market

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4099
Author(s):  
Ian Breakspear ◽  
Claudia Guillaume
Keyword(s):  
High Performance ◽  
Leaf Extract ◽  
Disease Risk ◽  
Respiratory Infections ◽  
Olive Leaf ◽  
Leaf Extracts ◽  
Over The Counter ◽  
Positive Effects ◽  
Risk Modifier ◽  
Phytochemical Profiles

Olive leaf extract (OLE), prepared from the fresh or dried leaves of Olea europaea L., is generating interest as a cardiovascular and metabolic disease risk modifier. Positive effects for the leaf extract and its key phytochemical constituents have been reported on blood pressure, respiratory infections, inflammation, and insulin resistance. A variety of OLE products are available both over-the-counter and for professional dispensing. The aim of this research was to quantitatively explore the phytochemical profile of different OLE products on the Australian market. Ten OLE products available on the Australian market (five over-the-counter products and five products for professional compounding and dispensing) were quantitatively analyzed for oleuropein, hydroxytyrosol, oleacein, oleocanthal, total biophenols, maslinic acid, and oleanolic acid, using high-performance liquid chromatography (HPLC). Substantial variation in oleuropein and hydroxytyrosol levels was noted between extracts, with a trend towards higher oleuropein and lower hydroxytyrosol levels being noted in products produced using the fresh olive leaf as opposed to dry olive leaf. These results suggest that OLE products on the Australian market vary substantially in their phytochemical profiles. Products for professional compounding and dispensing in many cases contained less oleuropein than over-the-counter products, but more hydroxytyrosol and comparable total biophenol levels.

scholarly journals Effect-Directed Profiling of Powdered Tea Extracts for Catechins, Theaflavins, Flavonols and Caffeine

Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 117 ◽  
Author(s):  
Gertrud E. Morlock ◽  
Julia Heil ◽  
Antonio M. Inarejos-Garcia ◽  
Jens Maeder
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Black Tea ◽  
Reversed Phase ◽  
Added Value ◽  
Leaf Extracts ◽  
Food Ingredients ◽  
Highly Active ◽  
Health Promoting ◽  
Natural Variance

The antioxidative activity of Camelia sinensis tea and especially powdered tea extracts on the market, among others used as added value in functional foods, can considerably vary due to not only natural variance, but also adulteration and falsification. Thus, an effect-directed profiling was developed to prove the functional effects or health-promoting claims. It took 3–12 min per sample, depending on the assay incubation time, for 21 separations in parallel. Used as a fast product quality control, it can detect known and unknown bioactive compounds. Twenty tea extracts and a reference mixture of 11-bioactive compounds were investigated in parallel under the same chromatographic conditions by a newly developed reversed phase high-performance thin-layer chromatographic method. In eight planar on-surface assays, effect-directed tea profiles were revealed. Catechins and theaflavins turned out to be not only highly active, but also multi-potent compounds, able to act in a broad range of metabolic pathways. The flavan-3-ols acted as radical scavengers (DPPH∙ assay), antibacterials against Gram-positive Bacillus subtilis bacteria, and inhibitors of tyrosinase, α-glucosidase, β-glucosidase, and acetylcholinesterase. Further effects against Gram-negative Aliivibrio fischeri bacteria and β-glucuronidase were assigned to other components in the powdered tea extracts. According to their specifications, the activity responses of the powdered tea extracts were higher than in mere leaf extracts of green, white and black tea. The multi-imaging and effect-directed profiling was not only able to identify known functional food ingredients, but also to detect unknown bioactive compounds (including bioactive contaminants, residues or adulterations).

scholarly journals Identification of Bioactive Compounds of Asparagus officinalis L.: Permutation Test Allows Differentiation among “Triguero” and Hybrid Green Varieties

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1640
Author(s):  
Cecilia Jiménez-Sánchez ◽  
Fabián Pedregosa ◽  
Isabel Borrás-Linares ◽  
Jesús Lozano-Sánchez ◽  
Antonio Segura-Carretero
Keyword(s):  
High Performance ◽  
Permutation Test ◽  
Asparagus Officinalis ◽  
Diode Array ◽  
Reverse Phase ◽  
Phytochemical Content ◽  
Flight Mass Spectrometry ◽  
Rp Hplc ◽  
Health Promoting ◽  
Phytochemical Profile

In this study, we determined the phytochemical profile of the Spanish “triguero” asparagus landrace “verde-morado” (Asparagus officinalis L.), a wild traditional landrace, and the improved “triguero” HT-801, together with two commercial green asparagus varieties. For comparison, we used reverse-phase high-performance liquid chromatography coupled with diode array electrospray time-of-flight mass spectrometry (RP-HPLC-DAD-ESI-TOF/MS) followed by a permutation test applied using a resampling methodology valid under a relaxed set of assumptions, such as i.i.d. errors (not necessarily normal) that are exchangeable under the null hypothesis. As a result, we postulate that “triguero” varieties (the improved HT-801 followed by its parent “verde-morado”) have a significantly different phytochemical profile from that of the other two commercial hybrid green varieties. In particular, we found compounds specific to the “triguero” varieties, such as feruloylhexosylhexose isomers, or isorhamnetin-3-O-glucoside, which was found only in the “triguero” variety HT-801. Although studies relating the phytochemical content of “triguero” asparagus varieties to its health-promoting effects are required, this characteristic phytochemical profile can be used for differentiating and revalorizating these asparagus cultivars.

scholarly journals Sulfoquinovose metabolism in marine algae

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Sabine Scholz ◽  
Manuel Serif ◽  
David Schleheck ◽  
Martin D.J. Sayer ◽  
Alasdair M. Cook ◽  
...  
Keyword(s):  
High Performance ◽  
Marine Algae ◽  
Model Organisms ◽  
Enzyme Assays ◽  
Laser Desorption Ionization ◽  
Genome Sequences ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Global Biogeochemical Cycles ◽  
Algal Extracts

Abstract This study aimed to survey algal model organisms, covering phylogenetically representative and ecologically relevant taxa. Reports about the occurrence of sulfonates (particularly sulfoquinovose, taurine, and isethionate) in marine algae are scarce, and their likely relevance in global biogeochemical cycles and ecosystem functioning is poorly known. Using both field-collected seaweeds from NW Scotland and cultured strains, a combination of enzyme assays, high-performance liquid chromatography and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry was used to detect key sulfonates in algal extracts. This was complemented by bioinformatics, mining the publicly available genome sequences of algal models. The results confirm the widespread presence of sulfonates and their biosynthetic pathways in macro- and microalgae. However, it is also clear that catabolic pathways, if present, must be different from those documented from the bacterial systems since no complete cluster of gene homologues of key genes could be detected in algal genomes.

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