scholarly journals A Quantitative Phytochemical Comparison of Olive Leaf Extracts on the Australian Market

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4099
Author(s):  
Ian Breakspear ◽  
Claudia Guillaume
Keyword(s):  
High Performance ◽  
Leaf Extract ◽  
Disease Risk ◽  
Respiratory Infections ◽  
Olive Leaf ◽  
Leaf Extracts ◽  
Over The Counter ◽  
Positive Effects ◽  
Risk Modifier ◽  
Phytochemical Profiles

Olive leaf extract (OLE), prepared from the fresh or dried leaves of Olea europaea L., is generating interest as a cardiovascular and metabolic disease risk modifier. Positive effects for the leaf extract and its key phytochemical constituents have been reported on blood pressure, respiratory infections, inflammation, and insulin resistance. A variety of OLE products are available both over-the-counter and for professional dispensing. The aim of this research was to quantitatively explore the phytochemical profile of different OLE products on the Australian market. Ten OLE products available on the Australian market (five over-the-counter products and five products for professional compounding and dispensing) were quantitatively analyzed for oleuropein, hydroxytyrosol, oleacein, oleocanthal, total biophenols, maslinic acid, and oleanolic acid, using high-performance liquid chromatography (HPLC). Substantial variation in oleuropein and hydroxytyrosol levels was noted between extracts, with a trend towards higher oleuropein and lower hydroxytyrosol levels being noted in products produced using the fresh olive leaf as opposed to dry olive leaf. These results suggest that OLE products on the Australian market vary substantially in their phytochemical profiles. Products for professional compounding and dispensing in many cases contained less oleuropein than over-the-counter products, but more hydroxytyrosol and comparable total biophenol levels.

scholarly journals Monitoring the Bioactive Compounds Status in Olea europaea According to Collecting Period and Drying Conditions

Energies ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 947 ◽  
Author(s):  
Amani Taamalli ◽  
Jesus Lozano Sánchez ◽  
Haifa Jebabli ◽  
Najla Trabelsi ◽  
Leila Abaza ◽  
...  
Keyword(s):  
High Performance ◽  
Reversed Phase ◽  
Olive Leaf ◽  
Leaf Extracts ◽  
Ionization Time ◽  
Drying Temperature ◽  
Flight Mass Spectrometry ◽  
Health Promoting ◽  
Drying Conditions ◽  
Phytochemical Profiles

Polyphenols and triterpenoids in olive have relevant importance both in the physiology of the plant and the nutritional and biological value of its products. Olive leaf extracts are of special interest for their numerous health-promoting properties. The present research is investigating the occurrence of phytochemicals in supercritical fluid extracts from leaves with regard to collection time and drying temperature. The phytochemical profiles of the olive leaf extracts were determined by reversed-phase high-performance liquid chromatography (HPLC) coupled to electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS) detection. The main extracted phytochemicals were phenols and terpenoids. A significant variation in the amounts of the different components was observed as a function of the different drying temperature and collecting time (p < 0.05). Among samples, the maximal contents of polyphenols and secoiridoid derivatives were found in the extracts from olive leaves collected in November and dried at 120 °C, whereas triterpenoids showed the highest content in fresh leaves collected in August.

scholarly journals Characterization and Bio-Accessibility Evaluation of Olive Leaf Extract-Enriched “Taralli”

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1268 ◽  
Author(s):  
Annamaria Cedola ◽  
Carmen Palermo ◽  
Diego Centonze ◽  
Matteo Alessandro Del Nobile ◽  
Amalia Conte
Keyword(s):  
Antioxidant Capacity ◽  
Leaf Extract ◽  
Total Content ◽  
White Wine ◽  
Olive Leaf ◽  
Olive Leaves ◽  
Ultrasound Assisted Extraction ◽  
Leaf Extracts ◽  
Digestion Process ◽  
Olive Leaf Extract

Olive leaves are rich in many compounds precious for human health. Due to this property, the current study was aimed to valorize the extract from this by-product in a cereal-based food, very popular all around the world, the “taralli”. To this aim, ultrasound-assisted extraction was applied to dried olive leaves to obtain the extract, used as “taralli” ingredient, instead of white wine. The “taralli” with and without extract was subjected to in vitro digestion to assess the quantity of polyphenolic compounds released in the gastrointestinal tract to become available for absorption. Total content of phenols and flavonoids, as well as the antioxidant capacity, was measured on both cooked and uncooked samples, before and after digestion. In addition, High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) of the three most abundant polyphenols present in olive leaf extracts, such as oleuropein, hydroxytyrosol, and verbascoside, was carried out at the three stages of the digestion process. The results showed that the substitution of white wine with olive leaf extract increased the total content of polyphenols and flavonoids and the antioxidant capacity. Bio-accessibility of the main phenolic compounds demonstrated that oleuropein resisted slightly after gastric digestion but was almost completely degraded in the intestinal phase, while hydroxytyrosol and verbascoside were not resistant to the digestion process from the gastric phase.

scholarly journals Assessment of the Efficacy of Olive Leaf (Olea europaea L.) Extracts in the Treatment of Colorectal Cancer and Prostate Cancer Using In Vitro Cell Models

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4069
Author(s):  
Sarah Albogami ◽  
Aziza  Hassan
Keyword(s):  
Prostate Cancer ◽  
Chlorogenic Acid ◽  
Cancer Cell ◽  
High Performance ◽  
Leaf Extract ◽  
Olive Leaf ◽  
Olive Leaf Extract ◽  
Anti Cancer

Cancer is one of the most serious public health issues worldwide, ranking second only to cardiovascular diseases as a cause of death. Numerous plant extracts have extraordinary health benefits and have been used for centuries to treat a variety of ailments with few side effects. Olive leaves have a long history of medicinal and therapeutic use. In this study, the anti-cancer properties of an olive leaf extract were investigated in vitro using colorectal and prostate cancer cell lines (HT29 and PC3, respectively). A high-performance liquid chromatography analysis showed that the olive leaf extract contained a high chlorogenic acid content. Accordingly, chlorogenic acid may be related to the observed effects of the aqueous extract on cancer cells, including increased inhibition of cancer cell growth, migration, DNA fragmentation, cell cycle arrest at the S phase, reactive oxygen species (ROS) production, and altered gene expression. The effects of the extracts were greater in HT29 than in PC3 cells. These results suggest that chlorogenic acid, the main constituent in the olive extract, is a promising new anti-cancer agent. Further analyses should focus on its in vivo effects on colorectal tumor models, both alone and in combination with established agents.

scholarly journals Thyroid-Modulating Activities of Olive and Its Polyphenols: A Systematic Review

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 529
Author(s):  
Kok-Lun Pang ◽  
Johanna Nathania Lumintang ◽  
Kok-Yong Chin
Keyword(s):  
Systematic Review ◽  
Olive Oil ◽  
Thyroid Function ◽  
Animal Studies ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Human Studies ◽  
Olive Pomace ◽  
Olive Leaf ◽  
Leaf Extracts

Olive oil, which is commonly used in the Mediterranean diet, is known for its health benefits related to the reduction of the risks of cancer, coronary heart disease, hypertension, and neurodegenerative disease. These unique properties are attributed to the phytochemicals with potent antioxidant activities in olive oil. Olive leaf also harbours similar bioactive compounds. Several studies have reported the effects of olive phenolics, olive oil, and leaf extract in the modulation of thyroid activities. A systematic review of the literature was conducted to identify relevant studies on the effects of olive derivatives on thyroid function. A comprehensive search was conducted in October 2020 using the PubMed, Scopus, and Web of Science databases. Cellular, animal, and human studies reporting the effects of olive derivatives, including olive phenolics, olive oil, and leaf extracts on thyroid function were considered. The literature search found 445 articles on this topic, but only nine articles were included based on the inclusion and exclusion criteria. All included articles were animal studies involving the administration of olive oil, olive leaf extract, or olive pomace residues orally. These olive derivatives were consistently demonstrated to have thyroid-stimulating activities in euthyroid or hypothyroid animals, but their mechanisms of action are unknown. Despite the positive results, validation of the beneficial health effects of olive derivatives in the human population is lacking. In conclusion, olive derivatives, especially olive oil and leaf extract, could stimulate thyroid function. Olive pomace residue is not suitable for pharmaceutical or health supplementation purposes. Therapeutic applications of olive oil and leaf extract, especially in individuals with hypothyroidism, require further validation through human studies.

scholarly journals PHYTOCHEMICAL STUDIES AND HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY ANALYSIS OF CALAMUS ROTANG LINN LEAF EXTRACTS

2018 ◽  
Vol 11 (2) ◽  
pp. 269
Author(s):  
Pallavi Y ◽  
Hemalatha Kpj
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Leaf Extract ◽  
Research Work ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Layer Chromatography ◽  
Ethanolic Leaf Extract

 Objective: The present study was aimed at phytochemical screening, quantification, and high-performance thin-layer chromatography (HPTLC) analysis of hexane, chloroform and ethanol leaf extracts of Calamus rotang.Methods: Leaf extracts were prepared according to the polarity of the solvents, i.e., hexane, chloroform, and ethanol. Preliminary phytochemical screening involved the qualitative methods to detect the presence of alkaloids, phenols, flavonoids, saponins, steroids, etc. Quantitative estimation of alkaloids using boldine as standard, phenols using gallic acid as standard, and flavonoids using quercetin as standard were done. HPTLC analysis was done with all three extracts along with quercetin and rutin standards using mobile phase for flavonoids, i.e., 90:10 ratio of chloroform and methanol solvents.Results: Phytochemical screening showed the presence of phenols, flavonoids, alkaloids, etc. Hence, quantification was done for these phytochemicals. Alkaloids were present significantly more in hexane leaf extract, i.e., 2.54±0.216mg boldine equivalents/g. Phenols were present significantly more in ethanolic leaf extract, i.e., 49.04±0.364 mg gallic acid equivalents)/g. Flavonoids were present in significant amount in ethanolic leaf extract, i.e., 458.85±5.74 mg quercetin equivalents/g. HPTLC analysis of hexane, chloroform, and ethanolic extracts showed the presence of flavonoids such as quercetin, rutin, and some unknown flavonoid compounds.Conclusion: Ethanolic leaf extract showed a high amount of phenols and flavonoids. Hence, the extract can be further exploited further for in vitro and in vivo research work.

Olive leaf extracts for shelf life extension of salmon burgers

2018 ◽  
Vol 25 (2) ◽  
pp. 91-100 ◽  
Author(s):  
Ibtihel Khemakhem ◽  
Ana Fuentes ◽  
María Jesús Lerma-García ◽  
Mohamed Ali Ayadi ◽  
Mohamed Bouaziz ◽  
...  
Keyword(s):  
Shelf Life ◽  
Leaf Extract ◽  
Life Extension ◽  
Total Volatile ◽  
Olive Leaf ◽  
Leaf Extracts ◽  
Life Study ◽  
Olive Leaf Extract ◽  
Fish Product

In this work, the effect of the addition of olive leaf extracts on the quality of vacuum-packed salmon burgers stored at 4 ℃ during 16 days has been studied. Olive leaf extract and its hydrolysate were initially characterized and then incorporated to salmon burgers. A shelf life study was conducted in three different batches (control, olive leaf extract, and hydrolyzed olive leaf extract burgers). Among the chemical indices determined, total volatile base nitrogen values were lower in hydrolyzed olive leaf extract and olive leaf extract burgers than in control samples. Lipid oxidation was lower in salmon burger with olive leaf extract. Salmon mince treated with hydrolyzed olive leaf extract showed lower microbial counts during the whole study, which extended the shelf life of the fish product. Therefore, the potential of olive leaf extracts to preserve salmon burgers during cold storage has been demonstrated.

Potential Herb-Drug Pharmacokinetic Interactions between African Wild Olive Leaf Extract and Selected Antihypertensive Drugs

Planta Medica ◽  
2018 ◽  
Vol 84 (12/13) ◽  
pp. 886-894
Author(s):  
Katlego Mmopele ◽  
Sandra Combrinck ◽  
Josias Hamman ◽  
Clarissa Willers ◽  
Weiyang Chen ◽  
...  
Keyword(s):  
Prescription Drugs ◽  
Leaf Extract ◽  
Antihypertensive Drugs ◽  
Blood Levels ◽  
Olive Leaf ◽  
Leaf Extracts ◽  
Olive Leaf Extract ◽  
Wild Olive

AbstractThe African wild olive (Olea europaea subsp. africana) is traditionally used as a hypotensive agent. Herb-drug interactions may result from the concurrent use of herbal medicines and conventional prescription drugs. This aspect was investigated by determining the effect of the extract on the in vitro intestinal epithelial permeation of selected hypotensive drugs using the Caco-2 cell culture model. The phytochemical profiles of leaf extracts of African wild olive from different localities in South Africa were compared, since efficacy is determined by the chemical composition. Extracts were analysed using ultra-performance liquid chromatography. The oleuropein concentration varied considerably from below the detection limit (4.94 µg/mL) to 59.4 mg/g dry weight. Chemometric models constructed from the aligned chromatographic data indicated only quantitative differences between the profiles. The leaf extract was found to increase the permeability of propranolol in the absorptive direction (Papp = 8.93 × 10−6 cm/s) across Caco-2 cell monolayers, but considerably decreased transport in the secretory direction (Papp = 3.68 × 10−6 cm/s). The permeation of diltiazem was enhanced by the extract in both the absorptive (Papp = 7.33 × 10−6 cm/s) as well as in the secretory direction (Papp = 7.16 × 10−6 cm/s), but a decrease in the efflux ratio was observed. The extract therefore caused a net increase in the transport of both drugs in the absorptive direction due to an inhibition effect on their efflux. This suggests a potential increase in the blood levels of these drugs when taken simultaneously with African wild olive leaf extract, indicating potential adverse effects that must be verified in vivo.

scholarly journals Olive Leaf Extract Modulates Quorum Sensing Genes and Biofilm Formation in Multi-Drug Resistant Pseudomonas aeruginosa

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 526
Author(s):  
Nazly R. El-sayed ◽  
Reham Samir ◽  
Lina Jamil M. Abdel-Hafez ◽  
Mohammed A. Ramadan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Green Tea ◽  
Biofilm Formation ◽  
Leaf Extract ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Olive Leaf ◽  
Leaf Extracts ◽  
Olive Leaf Extract

Biofilm acts as a complex barrier against antibiotics. In this study, we investigated the inhibitory activities of Olea europaea (olive) leaves Camellia sinensis (green tea), Styrax benzoin, Ocimum basilicum, Humulus lupulus, Ruta graveolens, and Propolis extracts on the biofilm formation, pyocyanin production, and twitching motility of Pseudomonas aeruginosa isolates. Moreover, we investigated the effect of olive leaf extract on the transcription of some biofilm related genes. A total of 204 isolates of Pseudomonas were collected from different Egyptian hospitals. A susceptibility test, carried out using the disc diffusion method, revealed that 49% of the isolates were multidrug-resistant. More than 90% of the isolates were biofilm-forming, of which 26% were strong biofilm producers. At subinhibitory concentrations, green tea and olive leaf extracts had the highest biofilm inhibitory effects with 84.8% and 82.2%, respectively. The expression levels of lasI, lasR, rhlI, and rhlR treated with these extracts were significantly reduced (p < 0.05) by around 97–99% compared to untreated isolates. This study suggests the ability of olive leaf extract to reduce the biofilm formation and virulence factor production of P. aeruginosa through the down regulation of quorum sensing (QS) genes. This may help in reducing our dependence on antibiotics and to handle biofilm-related infections of opportunistic pathogens more efficiently.

Analgesic and Anti-Inflammatory Property of the Methanol Extract from Ligustrum morrisonense Leaves in Rodents

2011 ◽  
Vol 39 (02) ◽  
pp. 335-348 ◽  
Author(s):  
Chi-Rei Wu ◽  
Wen-Hsin Lin ◽  
Yung-Ta Lin ◽  
Chi-Luan Wen ◽  
Hui Ching ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Array Detector ◽  
Leaf Extracts ◽  
Ligustrum Lucidum ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Anti Inflammatory

Ligustrum morrisonense Kaneh and Sasaki (abbreviated as LM), an endemic Ligustrum plant in Taiwan, is similar to Ligustrum lucidum, which is usually used for curing hepatic and inflammatory disorders. The aim of this study was to evaluate the analgesic and anti-inflammatory properties of LM by chemical-induced algesia and carrageenan-induced inflammation in rodents. Its triterpenoid contents were measured by using high performance liquid chromatography-photodiode array detector. LM leaf extracts effectively inhibited writhing responses induced by 1% acetic acid and biphasic-licking responses caused by 1% formalin. LM leaf extract also reduced the edema induced by 1% carrageenan. Furthermore, LM leaf extract reduced the abdominal Evan's blue extravasations caused by lipopolysaccharide (LPS), serotonin, histamine and bradykinin. LM leaf extract has higher contents of amyrin and lupeol among six assayed triterpenoid compounds. In conclusion, LM is a potential analgesic and anti-inflammatory Ligustrum plant, and its anti-inflammatory effects are partially related to decreasing microvascular permeability via inflammatory mediators and inhibiting cyclooxygenase-2 activity.

scholarly journals Immunomodulatory Activity of Electrospun Polyhydroxyalkanoate Fiber Scaffolds Incorporating Olive Leaf Extract

2021 ◽  
Vol 11 (9) ◽  
pp. 4006
Author(s):  
Jose Gustavo De la Ossa ◽  
Alessandra Fusco ◽  
Bahareh Azimi ◽  
Jasmine Esposito Salsano ◽  
Maria Digiacomo ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
High Performance ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Olive Tree ◽  
Immunomodulatory Activity ◽  
Burst Release ◽  
Olive Leaf ◽  
Olive Leaf Extract ◽  
Pro Inflammatory Cytokines

Olive tree is a well-known source of polyphenols. We prepared an olive leaf extract (OLE) and characterized it via high performance liquid chromatography (HPLC) analysis. OLE was blended with different polyhydroxyalkanoates (PHAs), namely, poly(hydroxybutyrate-co-hydroxyvalerate) (PHBHV) and polyhydroxybutyrate/poly(hydroxyoctanoate-co-hydroxydecanoate) (PHB/PHOHD), to produce fiber meshes via electrospinning: OLE/PHBV and OLE/ (PHB/PHOHD), respectively. An 80–90% (w/w%) release of the main polyphenols from the OLE/PHA fibers occurred in 24 h, with a burst release in the first 30 min. OLE and the produced fiber meshes were assayed using human dermal keratinocytes (HaCaT cells) to evaluate the expression of a panel of cytokines involved in the inflammatory process and innate immune response, such as the antimicrobial peptide human beta defensin 2 (HBD-2). Fibers containing OLE were able to decrease the expression of the pro-inflammatory cytokines at 6 h up to 24 h. All the PHA fibers allowed an early downregulation of the pro-inflammatory cytokines in 6 h, which is suggestive of a strong anti-inflammatory activity exerted by PHA fibers. Differently from pure OLE, PHB/PHOHD fibers (both with and without OLE) upregulated the expression of HBD-2. Our results showed that PHA fiber meshes are suitable in decreasing pro-inflammatory cytokines and the incorporation of OLE may enable indirect antibacterial properties, which is essential in wound healing and tissue regeneration.

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