scholarly journals PCOS and Assisted Reproduction Technique: Role and Relevance of Inositols

Endocrines ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 515-522
Author(s):  
Paolo Giovanni Artini ◽  
Elisa Malacarne ◽  
Vito Cela
Keyword(s):  
Ovarian Function ◽  
Polycystic Ovary ◽  
Obese Women ◽  
Assisted Reproduction Technique ◽  
Positive Role ◽  
Vitro Fertilization ◽  
Beneficial Effects ◽  
Insulin Sensitizers

Polycystic ovary syndrome is an endocrine disorder often characterized by insulin resistance and hyperinsulinemia, especially in overweight/obese women. Among insulin sensitizers, the positive role of inositols has been increasingly established in recent years. The action of inositols not only concerns the metabolic parameters of these patients, but also the hormonal profile, resulting in beneficial effects on ovarian function. For this reason, many studies have tried to recognize their role in PCOS infertile women who underwent in vitro fertilization (IVF) procedures.

503. SUCCESSFUL IN VITRO FERTILIZATION (IVF) AFTER PRIOR FAILED IVF UTILIZING A PROPRIETARY BLEND OF SUPPLEMENTS

2009 ◽  
Vol 21 (9) ◽  
pp. 104
Author(s):  
B. Poursharif ◽  
J. Paden ◽  
B. Acacio
Keyword(s):  
In Vitro Fertilization ◽  
Egg Quality ◽  
Clinical Pregnancy ◽  
Ivf Outcome ◽  
Positive Role ◽  
Vitro Fertilization ◽  
Embryo Fragmentation ◽  
History Of

Background: To date, little is known about the effect of supplements on the outcome of in vitro fertilization (IVF). The data on this matter is limited to measuring the overall pregnancy rate on a population of women who took a specific supplement, and not on IVF patients. Objectives: To demonstrate the positive role of an investigated supplement in the outcome of patients undergoing IVF. Method: 18 women undergoing IVF treatment were placed on a proprietary combination of vitamins and antioxidants designed to encourage blood flow and improve egg quality. The women were selected for this protocol mostly due to prior poor egg quality and/or large amount of embryo fragmentation .The women took supplementation twice daily for 4–12 weeks prior to transfer. The charts of the patients who used the supplements were used to obtain data. Previous failed IVF was defined as negative pregnancy. Successful IVF outcome was determined by positive chemical pregnancy and clinical pregnancy after one attempt. Range and mean was calculated for patient's age and number of failed previous IVF attempts. The previous IVF attempts were performed in different centers without using this supplement in all patients. Results: Eighteen patients used the supplement before and during their IVF cycles. Patient's age ranged from 28 to 44 with mean of 36.4 years. They had on average, 2 prior failed IVF attempts. Seventeen of 18 patients had successful IVF outcome. The failed patient required frozen testicular extraction of sperm (TESE), prior to IVF. Summary: Seventeen of 18 patients who used our supplements had successful IVF. These patients failed an average of 2 previous IVF attempts without using our supplements. Conclusion: Usage of our supplements is associated with improved rates of success in patients undergoing IVF with a history of prior failed IVF attempts. Larger studies need to be conducted.

scholarly journals Inositol Treatment and ART Outcomes in Women with PCOS

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Deepika Garg ◽  
Reshef Tal
Keyword(s):  
Ovarian Stimulation ◽  
Ovarian Function ◽  
Current Knowledge ◽  
Polycystic Ovary ◽  
Mature Oocyte ◽  
Reproductive Age ◽  
Vitro Fertilization ◽  
Ovulatory Dysfunction ◽  
Ovarian Morphology

Polycystic ovary syndrome (PCOS) affects 5–10% of women in reproductive age and is characterized by oligo/amenorrhea, androgen excess, insulin resistance, and typical polycystic ovarian morphology. It is the most common cause of infertility secondary to ovulatory dysfunction. The underlying etiology is still unknown but is believed to be multifactorial. Insulin-sensitizing compounds such as inositol, a B-complex vitamin, and its stereoisomers (myo-inositol and D-chiro-inositol) have been studied as an effective treatment of PCOS. Administration of inositol in PCOS has been shown to improve not only the metabolic and hormonal parameters but also ovarian function and the response to assisted-reproductive technology (ART). Accumulating evidence suggests that it is also capable of improving folliculogenesis and embryo quality and increasing the mature oocyte yield following ovarian stimulation for ART in women with PCOS. In the current review, we collate the evidence and summarize our current knowledge on ovarian stimulation and ART outcomes following inositol treatment in women with PCOS undergoing in vitro fertilization (IVF) and/or intracytoplasmic sperm injection (ICSI).

scholarly journals The Role of Follicular Fluid Anti-Mullerian Hormone in Success Rate of Intracytoplasmic Sperm Injection

2020 ◽  
Vol 8 (B) ◽  
pp. 962-965
Author(s):  
Hiathm Ahmed Baha El-Din ◽  
Essam Ahmed El Gindy ◽  
Ahmed Kotb Ahmed ◽  
Osama Ahmed Ibrahim ◽  
Ayman Nady Abdelmegeed
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Ovarian Function ◽  
Implantation Rate ◽  
Inverse Correlation ◽  
Clinical Pregnancy ◽  
University Hospital ◽  
Blastocyst Development ◽  
Vitro Fertilization

BACKGROUND: The role of anti-Mullerian hormone (AMH) in the ovary is to participate in the regulation of ovarian function, especially in follicle development and selection. It inhibits the initiation of human primordial follicle growth and prevents multiple selection of a dominant follicle by reducing the sensitivity of follicles to follicle stimulating hormone. MATERIALS AND METHODS: In this prospective clinical trial, outcomes were followed in 60 women undergoing cycles of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) within El-Minia University Hospital. AMH concentration was estimated in pooled follicle fluid (FF) on day of oocyte pickup. Cycles were sorted into low and high groups according to median (50th centile) values of measurement. The fertilization rate (FR), implantation rate, blastocyst development, embryo quality, chemical pregnancy, clinical pregnancy, and ongoing pregnancy after ICSI were counted as the main outcomes. RESULTS: Low FF AMH group shows significantly higher percentage of top-quality oocytes (67.1 ± 24.3 vs. 49.6 ± 30.3 %, p = 0.014), FR (83.9 ± 20.9 vs. 72.4 ± 21.4%, p = 0.021), clinical pregnancy (57.57 vs. 16.67%, p > 0.0001), and embryo implantation rates (57.7 vs. 16.7%, p = 0.001) compared to high FF AMH group. FF AMH shares an inverse correlation with FF E2 (Pearson r = −0.409, p < 0.001) and clinical pregnancy (Pearson r = −0.618, p < 0.001). Threshold value of FF AMH for pregnancy is >1.75 ng/mg protein. CONCLUSION: FF AMH is a plausible specific indicator of functional viability and quality of oocyte in IVF cycles.  

scholarly journals Suplementación de vitamina D en embarazadas y en mujeres con patologías ginecológicas

2021 ◽  
Vol 81 (04) ◽  
pp. 377-389
Author(s):  
Jorly Mejía-Montilla ◽  
◽  
Eduardo Reyna-Villasmil ◽  
María Albán-Andrade ◽  
Mariela Meza-Lozada ◽  
...  
Keyword(s):  
Vitamin D ◽  
Bone Mineralization ◽  
Vitamin D Supplementation ◽  
Dietary Intakes ◽  
Vitro Fertilization ◽  
Beneficial Effects ◽  
Adverse Pregnancy ◽  
Reproductive Processes

Apart from the well-known effects of vitamin D in maintaining calcium homeostasis and promoting bone mineralization, there is evidence to suggest that it modulates reproductive processes. About 70% adults have vitamin D deficiencies and there is a gap between recommended dietary intakes and deficient supply in general population. Observational studies show that vitamin D deficiency is a risk marker for reducing fertility and resulting adverse pregnancy. Evidence suggests that it could have beneficial effects on metabolic, hormonal and cellular parameters of polycystic ovarian syndrome, uterine myomatosis and endometriosis, while it seems to be associated with improvements in outcome of in vitro fertilization. The objective of this review was to evaluate the data of current literature on the role of vitamin D supplementation in pregnant women and in women with gynecological pathologies. Keywords: Vitamin D, Supplementation, Pregnancy, Endometriosis, Uterine myomatosis, Infertility.

scholarly journals In vitro Fertilization outcome in Patients with Polycystic Ovary Syndrome: Role of Age and Maternal Body Weight

2021 ◽  
Vol 6 (4) ◽  
pp. 161-166
Author(s):  
Shahrzad Zademodares ◽  
◽  
Masoumeh Abbaspour ◽  
Maryam Anbarluei ◽  
Nayereh Rahmati ◽  
...  
Keyword(s):  
Body Weight ◽  
In Vitro Fertilization ◽  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Ovary Syndrome ◽  
Maternal Body ◽  
Vitro Fertilization

scholarly journals Inositols: From Established Knowledge to Novel Approaches

2021 ◽  
Vol 22 (19) ◽  
pp. 10575
Author(s):  
Simona Dinicola ◽  
Vittorio Unfer ◽  
Fabio Facchinetti ◽  
Christophe O. Soulage ◽  
Nicholas D. Greene ◽  
...  
Keyword(s):  
Polycystic Ovary ◽  
Physiological Role ◽  
Reproductive Disorders ◽  
Beneficial Effects ◽  
Specific Effects ◽  
Insulin Sensitizers ◽  
Thecal Cells ◽  
Healthy State ◽  
Testosterone Biosynthesis

Myo-inositol (myo-Ins) and D-chiro-inositol (D-chiro-Ins) are natural compounds involved in many biological pathways. Since the discovery of their involvement in endocrine signal transduction, myo-Ins and D-chiro-Ins supplementation has contributed to clinical approaches in ameliorating many gynecological and endocrinological diseases. Currently both myo-Ins and D-chiro-Ins are well-tolerated, effective alternative candidates to the classical insulin sensitizers, and are useful treatments in preventing and treating metabolic and reproductive disorders such as polycystic ovary syndrome (PCOS), gestational diabetes mellitus (GDM), and male fertility disturbances, like sperm abnormalities. Moreover, besides metabolic activity, myo-Ins and D-chiro-Ins deeply influence steroidogenesis, regulating the pools of androgens and estrogens, likely in opposite ways. Given the complexity of inositol-related mechanisms of action, many of their beneficial effects are still under scrutiny. Therefore, continuing research aims to discover new emerging roles and mechanisms that can allow clinicians to tailor inositol therapy and to use it in other medical areas, hitherto unexplored. The present paper outlines the established evidence on inositols and updates on recent research, namely concerning D-chiro-Ins involvement into steroidogenesis. In particular, D-chiro-Ins mediates insulin-induced testosterone biosynthesis from ovarian thecal cells and directly affects synthesis of estrogens by modulating the expression of the aromatase enzyme. Ovaries, as well as other organs and tissues, are characterized by a specific ratio of myo-Ins to D-chiro-Ins, which ensures their healthy state and proper functionality. Altered inositol ratios may account for pathological conditions, causing an imbalance in sex hormones. Such situations usually occur in association with medical conditions, such as PCOS, or as a consequence of some pharmacological treatments. Based on the physiological role of inositols and the pathological implications of altered myo-Ins to D-chiro-Ins ratios, inositol therapy may be designed with two different aims: (1) restoring the inositol physiological ratio; (2) altering the ratio in a controlled way to achieve specific effects.

Activation of bovine oocytes by protein synthesis inhibitors: new findings on the role of MPF/MAPKs†

2021 ◽  
Author(s):  
Cecilia Valencia ◽  
Felipe Alonso Pérez ◽  
Carola Matus ◽  
Ricardo Felmer ◽  
María Elena Arias
Keyword(s):  
Protein Synthesis ◽  
Kinase Activity ◽  
Cyclin B1 ◽  
Protein Synthesis Inhibitors ◽  
Vitro Fertilization ◽  
New Findings ◽  
Bovine Oocytes ◽  
Dependent Pathway

Abstract The present study evaluated the mechanism by which protein synthesis inhibitors activate bovine oocytes. The aim was to analyze the dynamics of MPF and MAPKs. MII oocytes were activated with ionomycin (Io), ionomycin+anisomycin (ANY) and ionomycin+cycloheximide (CHX) and by in vitro fertilization (IVF). The expression of cyclin B1, p-CDK1, p-ERK1/2, p-JNK, and p-P38 were evaluated by immunodetection and the kinase activity of ERK1/2 was measured by enzyme assay. Evaluations at 1, 4, and 15 hours postactivation (hpa) showed that the expression of cyclin B1 was not modified by the treatments. ANY inactivated MPF by p-CDK1Thr14-Tyr15 at 4 hpa (P &lt; 0.05), CHX increased pre-MPF (p-CDK1Thr161 and p-CDK1Thr14-Tyr15) at 1 hpa and IVF increased p-CDK1Thr14-Tyr15 at 17 hours postfertilization (hpf) (P &lt; 0.05). ANY and CHX reduced the levels of p-ERK1/2 at 4 hpa (P &lt; 0.05) and its activity at 4 and 1 hpa, respectively (P &lt; 0.05). Meanwhile, IVF increased p-ERK1/2 at 6 hpf (P &lt; 0.05); however, its kinase activity decreased at 6 hpf (P &lt; 0.05). p-JNK in ANY, CHX, and IVF oocytes decreased at 4 hpa (P &lt; 0.05). p-P38 was only observed at 1 hpa, with no differences between treatments. In conclusion, activation of bovine oocytes by ANY, CHX, and IVF inactivates MPF by CDK1-dependent specific phosphorylation without cyclin B1 degradation. ANY or CHX promoted this inactivation, which seemed to be more delayed in the physiological activation (IVF). Both inhibitors modulated MPF activity via an ERK1/2-independent pathway, whereas IVF activated the bovine oocytes via an ERK1/2-dependent pathway. Finally, ANY does not activate the JNK and P38 kinase pathways.

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