scholarly journals Molecular Mechanisms through Which Short-Term Cold Storage Improves the Nutritional Quality and Sensory Characteristics of Postharvest Sweet Potato Tuberous Roots: A Transcriptomic Study

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2079
Author(s):  
Shuqian Zhou ◽  
Lu Chen ◽  
Gang Chen ◽  
Yongxin Li ◽  
Huqing Yang
Keyword(s):  
Chlorogenic Acid ◽  
Sweet Potato ◽  
Cold Storage ◽  
Nutritional Quality ◽  
Molecular Mechanisms ◽  
Chilling Injury ◽  
Short Term ◽  
Tuberous Roots ◽  
Significant Difference ◽  
Transcriptome Analyses

Sweet potato (Ipomoea batatas (L.) Lam.) is a commercially relevant food crop with high demand worldwide. This species belongs to the Convolvulaceae family and is native to tropical and subtropical regions. Storage temperature and time can adversely affect tuberous roots' quality and nutritional profile. Therefore, this study evaluates the effect of storage parameters using physicochemical and transcriptome analyses. Freshly harvested tuberous roots (Xingxiang) were stored at 13 °C (control) or 5 °C (cold storage, CS) for 21 d. The results from chilling injury (CI) evaluation demonstrated that there was no significant difference in appearance, internal color, weight, and relative conductivity between tuberous roots stored at 13 and 5 °C for 14 d and indicated that short-term CS for 14 d promoted the accumulation of sucrose, chlorogenic acid, and amino acids with no CI symptoms development. This, in turn, improved sweetness, antioxidant capacity, and nutritional value of the tuberous roots. Transcriptome analyses revealed that several key genes associated with sucrose, chlorogenic acid, and amino acid biosynthesis were upregulated during short-term CS, including sucrose synthase, sucrose phosphate synthase, phenylalanine ammonia-lyase, 4-coumarate-CoA ligase, hydroxycinnamoyl-CoA quinate hydroxycinnamoyltransferase, serine hydroxymethyltransferase, alanine aminotransferase, arogenate dehydrogenase, and prephenate dehydratase. These results indicated that storage at 5 °C for 14 d could improve the nutritional quality and palatability of sweet potato tuberous roots without compromising their freshness.

scholarly journals Transcriptome Analysis of Pre-Storage 1-MCP and High CO2-Treated ‘Madoka’ Peach Fruit Explains the Reduction in Chilling Injury and Improvement of Storage Period by Delaying Ripening

2021 ◽  
Vol 22 (9) ◽  
pp. 4437
Author(s):  
Han Ryul Choi ◽  
Min Jae Jeong ◽  
Min Woo Baek ◽  
Jong Hang Choi ◽  
Hee Cheol Lee ◽  
...  
Keyword(s):  
Cold Storage ◽  
Transcriptome Analysis ◽  
Molecular Mechanisms ◽  
Chilling Injury ◽  
Chemical Properties ◽  
Storage Period ◽  
Differentially Expressed ◽  
Peach Fruit ◽  
High Co2 ◽  
Physico Chemical

Cold storage of peach fruit at low temperatures may induce chilling injury (CI). Pre-storage 1-MCP and high CO2 treatments were reported among the methods to ameliorate CI and reduce softening of peach fruit. However, molecular data indicating the changes associated with pre-storage 1-MCP and high CO2 treatments during cold storage of peach fruit are insufficient. In this study, a comparative analysis of the difference in gene expression and physico-chemical properties of fruit at commercial harvest vs. stored fruit for 12 days at 0 °C (cold-stored (CS), pre-storage 1-MCP+CS, and pre-storage high CO2+CS) were used to evaluate the variation among treatments. Several genes were differentially expressed in 1-MCP+CS- and CO2+CS-treated fruits as compared to CS. Moreover, the physico-chemical and sensory data indicated that 1-MCP+CS and CO2+CS suppressed CI and delayed ripening than the CS, which could lead to a longer storage period. We also identified the list of genes that were expressed commonly and exclusively in the fruit treated by 1-MCP+CS and CO2+CS and compared them to the fruit quality parameters. An attempt was also made to identify and categorize genes related to softening, physiological changes, and other ripening-related changes. Furthermore, the transcript levels of 12 selected representative genes from the differentially expressed genes (DEGs) in the transcriptome analysis were confirmed via quantitative real-time PCR (qRT-PCR). These results add information on the molecular mechanisms of the pre-storage treatments during cold storage of peach fruit. Understanding the genetic response of susceptible cultivars such as ‘Madoka’ to CI-reducing pre-storage treatments would help breeders release CI-resistant cultivars and could help postharvest technologists to develop more CI-reducing technologies.

scholarly journals Effect of 1-MCP, ethylene absorber and ozone on melon quality during storage

2018 ◽  
Vol 14 (s1) ◽  
pp. 101-110
Author(s):  
Lien Phuong Le Nguyen ◽  
Vivien Horváth ◽  
Mai Sao Dam ◽  
Géza Hitka ◽  
Tamás Zsom ◽  
...  
Keyword(s):  
Disease Severity ◽  
Cold Storage ◽  
Ethylene Production ◽  
Chilling Injury ◽  
Ozone Treatment ◽  
Surface Color ◽  
Respiration Rates ◽  
Significant Difference ◽  
And Control ◽  
Control Samples

This work was aimed to investigate the effect of 1-methylcyclopropene (1-MCP), ethylene absorber (EA), ozone alone or in combination on melon quality during storage. Ethylene production, respiration rates, acoustic firmness, surface color, chilling injury, and disease severity of melon were determined. 1-MCP treated fruits and non 1-MCP treated fruits were stored with sachets of ethylene absorber containing KMnO4 or ozone at 0.1 ppm/h during 10 days at 5 °C and subsequent 4 days at 20 °C. Melons treated with 1-MCP were firmer than the rest of the samples during storage. In addition, 1-MCP reduced the yellowing of melon rind compared to other treatments. The combination of 1-MCP and EA did not offer any additional effect in comparison with 1-MCP alone. There was no significant difference between fruits stored with ethylene absorber, ozone and control samples. Ozone treatment during cold storage decreased disease severity, however, fruits exposed to ozone had more serious decay throughout storage at 20 °C, probably due to the unclean air in the chamber.

RNA-seq reveals molecular mechanisms behind chilling injury tolerance in ISW-treated zucchini fruit during cold storage

2020 ◽  
pp. 149-154
Author(s):  
A. Chana-Muñoz ◽  
A. García ◽  
E. Aguado ◽  
J. Romero ◽  
G. Cebrián ◽  
...  
Keyword(s):  
Cold Storage ◽  
Molecular Mechanisms ◽  
Chilling Injury ◽  
Rna Seq ◽  
Injury Tolerance

scholarly journals Culling Tomatoes with External Symptoms of Irregular Ripening Is of Limited Benefit

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 316-317 ◽  
Author(s):  
Charles A. Powell ◽  
Peter J. Stoffella
Keyword(s):  
Lycopersicon Esculentum ◽  
Sweet Potato ◽  
Bemisia Tabaci ◽  
Cold Storage ◽  
Fresh Fruit ◽  
Green Fruit ◽  
Significant Difference ◽  
Almost All ◽  
Sweet Potato Whitefly

Mature-green and mature-red tomato (Lycopersicon esculentum Mill.) fruit were harvested from spring- and fall-grown plants infested with sweet potato whitefly (SPWF; Bemisia tabaci Gennadins). The mature-green fruit were either ripened at 20 to 22C or cold-stored at 10 to 13C for 3 weeks and then were allowed to ripen at 20 to 22C. There was no significant difference in the appearance of either external or internal tomato irregular ripening (TIR) symptoms between the two storage–ripening regimes or in the appearance of internal TIR symptoms among the two storage regimes and vine-ripened tomatoes. Thus, removing the tomatoes from the SPWF during ripening does not reduce TIR symptoms. About half of the mature-green tomatoes, ripened with or without cold storage (10 to 13C), developed no external TIR symptoms, but about half of these tomatoes had internal TIR symptoms. About one-third of the tomatoes developed external symptoms during ripening, but these symptoms disappeared after ripening was complete. A high percentage (71%) of these tomatoes with external symptoms also had internal symptoms. The remaining tomatoes developed external TIR that did not disappear, and almost all of these tomatoes had internal symptoms. These data suggest that culling tomatoes that develop external TIR during ripening will reduce but not eliminate tomatoes with internal TIR from the fresh-fruit market.

scholarly journals Studies of the Freezing of Green Plantains (Musa paradisiaca) IV. Effect of Cold Storage on the Quality of Frozen Sliced Green Plantains

1969 ◽  
Vol 59 (4) ◽  
pp. 239-244
Author(s):  
F. Sánchez Nieva ◽  
I. Hernández ◽  
C. E. Bueso
Keyword(s):  
Cold Storage ◽  
Starch Content ◽  
Chilling Injury ◽  
Storage Period ◽  
High Quality ◽  
Musa Paradisiaca ◽  
Significant Difference

Green plantains stored at 7° C (45° F) showed chilling injury about the 10th day. Slight changes in texture and a decrease in the starch content occurred during a storage period of 20 days. Dark spots in both peel and pulp caused by chilling injury affected the appearance of the fried tostones but did not lower the overall quality of the product. High quality tostones could be prepared from plantains stored at 13° C (55° F) for as long as 20 days. No significant difference could be found in the quality of tostones prepared from fruit stored at 7° and 13° C for 20 days. The time of storage had no effect on the yield of slices prepared from the refrigerated fruit at these temperatures.

scholarly journals Evaluation of suitability of substituting wheat flour with sweet potato and tiger nut flours in bread making

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Chinelo V. Ezeocha ◽  
Nnenna A. Onwuneme
Keyword(s):  
Sweet Potato ◽  
Wheat Flour ◽  
Nutritional Quality ◽  
Fibre Content ◽  
Partial Substitution ◽  
Loaf Volume ◽  
Bread Making ◽  
Crude Fibre ◽  
Potential Health ◽  
Significant Difference

AbstractThe study was carried out to assess the nutritional quality and palatability of bread after partially substituting wheat flour (WF) with sweet potato (SF) and tiger nut (TF) flours. Sweet potato (yellow fleshed) and tiger nut flours were used to substitute wheat flour at 5, 10, 15 and 20% level each. The bread quality of each of these composite flours evaluated on the basis of their nutritional quality, physical properties and acceptability by consumers. The findings indicate slight decrease in the protein content of the bread samples with increasing sweet potato and tiger nut supplementation (6.97% in 100% wheat flour to 3.80% in 20% level of substitution) while crude fibre content increased with increased sweet potato and tiger nut substitution (2.29% in 100% WF to 2.80% in 20% substitution) but there was no significant change (<0.05) in the ash and fat contents. Carbohydrate which was the major component ranged from 73.47 – 79.42%. Findings on the study on the functional properties showed that water absorption capacity increases with increase in the level of sweet potato and tiger nut substitution (0.54g/ml in 100% WF to 1.12g/ml in 20% level of substitution). The loaf volume and specific volume of the bread samples decreased significantly with increase in the level of substitution with sweet potato and tiger nut and it ranged from 320cm3 (in 100% wheat flour) – 216cm3 (in 20% substitution) and 1.55cm3/g (in 100% wheat flour) to 0.98cm3/g (in 20% level of substitution) respectively. Bread from 100% wheat flour scored the highest in all the sensory properties evaluated. There was no significant difference between the control and bread from 5% level of substitution in terms of all the sensory parameters evaluated however bread from higher levels of substitution varied significantly with the control. All the bread samples were acceptable in all the parameters evaluated except bread from 20% level of substitution. The study concludes the potential health and sensory benefits of partial substitution of sweet potato and tiger nut to wheat flour in bread making through improved fibre content, improved taste and to promote sweet potato and tiger nut utilization.

scholarly journals Corrigendum to: Efficacy of short-term cold storage prior to cryopreservation of spermatozoa in a threatened lizard

2021 ◽  
Vol 33 (9) ◽  
pp. 619
Author(s):  
Lachlan Campbell ◽  
John Clulow ◽  
Belinda Howe ◽  
Rose Upton ◽  
Sean Doody ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Significant Role ◽  
Cold Storage ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Motile Sperm ◽  
Sperm Viability ◽  
Short Term ◽  
Significant Difference

Assisted reproductive technologies (ARTs) have a significant role to play in reptile conservation, yet are severely lacking. Previous attempts to cryopreserve spermatozoa in the threatened lizard Varanus panoptes achieved approximately 48% motile sperm post-thaw for samples frozen immediately after collection. However, the feasibility of extended cold storage before cryopreservation has not been tested. We held V. panoptes spermatozoa at either 25°C or 4°C for 8 days, assessing sperm motility at days 1, 2, 4 and 8. Subsamples were cryopreserved on days 1 and 4 following the previously reported protocol for this species. Percentage motility decreased rapidly at 25°C, but did not decrease significantly until 4 days after collection at 4°C, with &gt;30% motility maintained after 8 days. There was no significant difference in post-thaw motility or viability of samples cryopreserved after 1 or 4 days storage at 4°C, yielding substantial results for both parameters (mean motility 23.8% and 28.1% and mean viability 50.1% and 57.5% after 1 and 4 days respectively). We demonstrate the capacity to extend sperm viability for up to 8 days in unfrozen samples and to produce acceptable post-thaw motility in samples frozen after 4 days of storage, contributing to the development of valuable ARTs for lizards and other reptiles.

scholarly journals Integrated transcriptome, small RNA and degradome sequencing approaches proffer insights into chlorogenic acid biosynthesis in leafy sweet potato

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245266
Author(s):  
Yi Liu ◽  
Wenjin Su ◽  
Lianjun Wang ◽  
Jian Lei ◽  
Shasha Chai ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Sweet Potato ◽  
Small Rna ◽  
In Silico ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Ethylene Response Factor ◽  
Sequencing Data ◽  
Degradome Sequencing ◽  
Phenylpropanoid Biosynthesis

Leafy sweet potato is rich in total phenolics (TP) which play key roles in health protection, the chlorogenic acid (CGA) constitutes the major components of phenolic compounds in leafy sweet potato. Unfortunately, the mechanism of CGA biosynthesis in leafy sweet potato is unclear. To dissect the mechanisms of CGA biosynthesis, we performed transcriptome, small RNA (sRNA) and degradome sequencing of one low-CGA content and one high-CGA content genotype at two stages. A total of 2,333 common differentially expressed genes (DEGs) were identified, and the enriched DEGs were related to photosynthesis, starch and sucrose metabolism and phenylpropanoid biosynthesis. The functional genes, such as CCR, CCoAOMT and HCT in the CGA biosynthetic pathway were down-regulated, indicating that the way to lignin was altered, and two possible CGA biosynthetic routes were hypothesized. A total of 38 DE miRNAs were identified, and 1,799 targets were predicated for 38 DE miRNAs by using in silico approaches. The target genes were enriched in lignin and phenylpropanoid catabolic processes. Transcription factors (TFs) such as apetala2/ethylene response factor (AP2/ERF) and Squamosa promoter binding protein-like (SPL) predicated in silico were validated by degradome sequencing. Association analysis of the DE miRNAs and transcriptome datasets identified that miR156 family negatively targeted AP2/ERF and SPL. Six mRNAs and six miRNAs were validated by qRT-PCR, and the results showed that the expression levels of the mRNAs and miRNAs were consistent with the sequencing data. This study established comprehensive functional genomic resources for the CGA biosynthesis, and provided insights into the molecular mechanisms involving in this process. The results also enabled the first perceptions of the regulatory roles of mRNAs and miRNAs, and offered candidate genes for leafy sweet potato improvements.

Sign in / Sign up

Export Citation Format

Share Document