scholarly journals A Rapid Method for the Identification of Fresh and Processed Pagellus erythrinus Species against Frauds

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1397
Author(s):  
Marina Ceruso ◽  
Celestina Mascolo ◽  
Pasquale De Luca ◽  
Iolanda Venuti ◽  
Giorgio Smaldone ◽  
...  
Keyword(s):  
Fish Species ◽  
Hamming Distance ◽  
Specific Primers ◽  
Pagellus Erythrinus ◽  
Fish Species Identification ◽  
The Family ◽  
The Common ◽  
Processed Products ◽  
Species Specific ◽  
Complete Mtdna

The commercialization of porgies or seabreams of the family Sparidae has greatly increased in the last decade, and some valuable species have become subject to seafood substitution. DNA regions currently used for fish species identification in fresh and processed products belong to the mitochondrial (mt) genes cytochrome b (Cytb), cytochrome c oxidase I (COI), 16S and 12S. However, these markers amplify for fragments with lower divergence within and between some species, failing to provide informative barcodes. We adopted comparative mitogenomics, through the analysis of complete mtDNA sequences, as a compatible approach toward studying new barcoding markers. The intent is to develop a specific and rapid assay for the identification of the common pandora Pagellus erythrinus, a sparid species frequently subject to fraudulent replacement. The genetic diversity analysis (Hamming distance, p-genetic distance, gene-by-gene sequence variability) between 16 sparid mtDNA genomes highlighted the discriminating potential of a 291 bp NAD2 gene fragment. A pair of species-specific primers were successfully designed and tested by end-point and real-time PCR, achieving amplification only in P. erythrinus among several fish species. The use of the NAD2 barcoding marker provides a rapid presence/absence method for the identification of P. erythrinus.

Rapid Fish Species Identification by Agarose Gel Isoelectric Focusing of Sarcoplasmic Proteins

1981 ◽  
Vol 64 (1) ◽  
pp. 38-43
Author(s):  
Ronald C Lundstrom
Keyword(s):  
Species Identification ◽  
Isoelectric Focusing ◽  
Fish Species ◽  
Protein Pattern ◽  
Agarose Gel ◽  
Tissue Fluid ◽  
Protein Patterns ◽  
Fish Species Identification ◽  
Species Specific ◽  
Gel Isoelectric Focusing

Abstract A rapid method is described for fish species identification by agarose gel isoelectric focusing (AGIEF). The AGIEF method can be completed in less than 2 h and gives reproducible species-specific sarcoplasmic protein patterns. Protein patterns are similar using either centrifuged tissue fluid or muscle tissue as the sample. One species, monkfish (Lophius americanus), has a polymorphic protein pattern. A predominant pattern was found in 66.7% of the individuals; 2 variant patterns were equally distributed among the remaining 33.3%. AGIEF offers a more rapid, less expensive alternative to the current AOAC official first action method for fish species identification based on polyacrylamide gel isoelectric focusing.

Liquid Chromatographic Identification of Common Fish Species

1987 ◽  
Vol 70 (4) ◽  
pp. 618-625 ◽  
Author(s):  
Magdi A Osman ◽  
Samy H Ashoor ◽  
Paul C Marsh
Keyword(s):  
Fish Species ◽  
Water Soluble ◽  
Simple Liquid ◽  
Fresh Fish ◽  
Qualitative And Quantitative ◽  
Fish Species Identification ◽  
Total Analysis ◽  
Species Specific ◽  
Fish Proteins ◽  
Liquid Chromatographic

Abstract Water-soluble sarcoplasmic proteins in aqueous blends of each of 31 fresh fish species were separated and quantitated by a simple liquid chromatographic (LC) method. The method revealed major qualitative and quantitative differences which were used for reliable identification of all species. Freezing for 6 months had no apparent effect on water-soluble fish proteins and did not change species-specific chromatograms. Cooking, however, caused significant changes which resulted in chromatograms different from those of fresh or frozen samples. Fish species identification by the LC method is rapid (total analysis time is about 1 h) and reliable and does not require special skills. It is applicable to fresh and frozen whole, sliced, filleted, and minced fish.

scholarly journals Species-Specific Primers for Eutypella parasitica, the Causal Agent of Eutypella Canker of Maple

Plant Disease ◽  
2007 ◽  
Vol 91 (12) ◽  
pp. 1579-1584 ◽  
Author(s):  
Barbara Piškur ◽  
Nikica Ogris ◽  
Dušan Jurc
Keyword(s):  
Large Scale ◽  
Fragment Size ◽  
Intraspecific Variability ◽  
Its Region ◽  
Specific Primers ◽  
Eutypa Lata ◽  
Wide Range ◽  
The Family ◽  
Species Specific ◽  
First Time

Eutypella parasitica was recently reported in Europe for the first time, and this study reports the molecular evaluation of the internal transcribed spacer (ITS)1/5.8S/ITS2 regions of 68 isolates of the fungus obtained in pure culture with polymerase chain reaction restriction fragment length polymorphism (RFLP). The RFLP patterns of all isolates proved identical and the restriction profiles served to differentiate E. parasitica from Eutypa lata, another pathogenic member of the family Diatrypaceae. Low intraspecific variability was detected in the sequenced ITS1/5.8S/ITS2 regions of eight Eutypella parasitica isolates originating from different hosts and geographical locations. Based on this ITS region, EpR/F primers specific to E. parasitica were constructed and tested with a wide range of fungal isolates. The EpR/F primer pair successfully amplified the expected fragment size of 341 bp from isolates of E. parasitica and also directly from infected maple wood shavings. The RFLP patterns and species-specific primers represent a step toward routine, large-scale, and rapid molecular diagnostics and identification of E. parasitica.

Molecular detection of Moniezia spp. (Cestoda) in Pergalumna persica (Acari: Oribatida) in Iran

2018 ◽  
Vol 23 (10) ◽  
pp. 1931
Author(s):  
Mohammad Ali Akrami ◽  
Reza MOSTOWFIZADEH-GHALAMFARSA ◽  
Forough Ebrahimi ◽  
Mohammad Moazeni
Keyword(s):  
Ribosomal Rna ◽  
First Record ◽  
Oribatid Mites ◽  
Fars Province ◽  
Intermediate Hosts ◽  
Artificial Infection ◽  
Specific Primers ◽  
18S Ribosomal Rna Gene ◽  
The Common ◽  
Species Specific

Some oribatid mites are intermediate hosts of anoplocephalid cestodes, where the cestodes complete their larval development in the bodies of these oribatid mites. In order to investigate the potential of oribatid mites as vectors of anoplocephalid cestodes, 64 species of these mites were collected from different pastures of Shiraz County (Fars Province, Iran) during 2010–12. Two predominant species, Pergalumna persica and Acrogalumna lanceolata, were selected for study of both laboratory and natural infections. For artificial infection, these two species were exposed separately to eggs of Moniezia expansa and Moniezia benedeni, which are the common anoplocephalid cestodes of sheep and cattle in the Shiraz region. In order to develop a PCR-based technique for identification and detection of these cestodes, species-specific primers were designed to amplify the 18S ribosomal RNA gene. We demonstrate that the mite P. persica can be infested by M. expansa and M. benedeni in the laboratory and it can be naturally infected by the former species; however cestodes were not detected in the mite A. lanceolata. This is the first record of P. persica as the intermediate host of anoplocephalid tapeworms. 

scholarly journals Normalization of Active Appearance Models for Fish Species Identification

2011 ◽  
Vol 2011 ◽  
pp. 1-16 ◽  
Author(s):  
Charles-Henri Quivy ◽  
Itsuo Kumazawa
Keyword(s):  
Species Identification ◽  
Fish Species ◽  
Computational Cost ◽  
Appearance Model ◽  
Active Appearance Model ◽  
Active Appearance Models ◽  
Fish Species Identification ◽  
Novel Method ◽  
Active Appearance ◽  
Species Specific

In recent years, automatic visual coral reef monitoring has been proposed to solve the demerits of manual monitoring techniques. This paper proposes a novel method to reduce the computational cost of the standard Active Appearance Model (AAM) for automatic fish species identification by using an original multiclass AAM. The main novelty is the normalization of species-specific AAMs using techniques tailored to meet with fish species identification. Shape models associated to species-specific AAMs are automatically normalized by means of linear interpolations and manual correspondences between shapes of different species. It leads to a Unified Active Appearance Model built from species that present characteristic texture patterns. Experiments are carried out on images of fish of four different families. The technique provides correct classification rates up to 92% on 5 species and 84.5% on 12 species and is more than 4 times faster than the standard AAM on 12 species.

scholarly journals A molecular survey based on eDNA to assess the presence of a clown featherback (Chitala ornata) in a confined environment

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10338
Author(s):  
Maslin Osathanunkul ◽  
Toshifumi Minamoto
Keyword(s):  
Developing Countries ◽  
Food Security ◽  
Rural Communities ◽  
Fish Species ◽  
Water Samples ◽  
Specific Primers ◽  
Inland Fisheries ◽  
Species Specific ◽  
Confined Environment ◽  
Important Fish

Background The importance of the inland fisheries sector in food security as a provider of much-needed protein and income supplier has been highlighted. This is especially the case in poor rural communities in developing countries. Inland capture fisheries in Thailand are in place nationwide in rivers, lakes, swamps and reservoirs. The clown featherback (Chitala ornata) is popularly consumed and is an economically important fish in Thailand which is often used in food products such as fish balls and fish cakes. Along with other fish species, the clown featherback is one of fish of inland fisheries at Phayao Lake. Recent fish surveys from 2016-2018 at Phayao Lake using netting and electrofishing found that the number of clown featherback have been reducing since 2016 and could not be detected at all by 2018. This is despite the fact that there are still reports of their presence in the lake from locals. Methods We developed an eDNA-based method for detection of the clown featherback in Phayao Lake as an alternative tool. Water samples were collected in three different sampling months (February, June and September) at six sites located in the lake. Species-specific primers and the probe were designed to amplify a 183 bp fragment of the cytB region of the clown featherback. Results eDNA of the clown featherback can be detected in all different sampling months and sites. Concentration of the clown featherback found in Prayao Lake showed no difference over sampling month but between collecting sites. This proves that eDNA based survey is a sensitive and useful tool for monitoring and surveying the clown featherback at any time of the year.

scholarly journals Evolution, Expression, and Function of Gonadal Somatic Cell-Derived Factor

2021 ◽  
Vol 9 ◽  
Author(s):  
Chen-wei Hsu ◽  
Bon-chu Chung
Keyword(s):  
Somatic Cell ◽  
Fish Species ◽  
Ovarian Development ◽  
Developmental Stages ◽  
Somatic Cells ◽  
Specific Factors ◽  
The Common ◽  
Male Sex ◽  
Species Specific ◽  
And Function

Fish gonads develop in very diverse ways different from mammalian gonads. This diversity is contributed by species-specific factors. Gonadal somatic cell-derived factor (Gsdf) is one such factor. The gsdf gene exists mostly in teleosts and is absent in many tetrapods, probably as a result of two gene losses during evolution. The gsdf transcript is expressed mainly in gonadal somatic cells, including Sertoli cell in testis and granulosa cells in ovary; however, these gonadal somatic cells can surround many types of germ cells at different developmental stages depending on the fish species. The function of gsdf is also variable. It is involved in germ cell proliferation, testicular formation, ovarian development and even male sex determination. Here, we summarize the common and diverse expression, regulation and functions of gsdf among different fish species with aspect of evolution.

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