scholarly journals Salt Tolerance of Hydrangea Plants Varied among Species and Cultivar within a Species

Horticulturae ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 54 ◽  
Author(s):  
Genhua Niu ◽  
Youping Sun ◽  
Triston Hooks ◽  
James Altland ◽  
Haijie Dou ◽  
...  

A greenhouse study was conducted to assess the relative salt tolerance of 11 cultivars of hydrangea: Hydrangea macrophylla ‘Ayesha’, ‘Emotion’, ‘Mathilda Gutges’, ‘Merritt’s Supreme’ and ‘Passion’; H. paniculata ‘Interhydia’ and ‘Bulk’; H. quercifolia ‘Snowflake’; H. serrata ‘Preciosa’; and H. serrata × macrophylla ‘Sabrina’ and ‘Selina’. Plants were treated with a nutrient solution at an electrical conductivity (EC) of 1.0 dS·m−1, and nutrient solution-based saline solutions at an EC of 5.0 dS·m−1 (EC 5) or 10 dS·m−1 (EC 10). The study was repeated in time (Experiments 1 and 2). In both experiments, by the fourth week after treatment, ‘Bulk’ plants in EC 10 exhibited severe salt damage with most of them dead. ‘Interhydia’ was also sensitive, showing severe salt damage in EC 10 with a high mortality rate by the end of the experiment. The leaf area and total shoot dry weight (DW) of all cultivars in EC 5 and EC 10 treatments were significantly reduced compared to the control. Leaf sodium (Na+) and chloride (Cl−) concentrations were negatively correlated with visual quality, leaf area and shoot DW. The salt-sensitive cultivars ‘Bulk’, ‘Interhydia’ and ‘Snowflake’ had inherently low leaf Na+ and Cl− concentrations in both control and salt-treated plants compared to other cultivars. Salt tolerance varied among species and cultivars within H. macrophylla. Among the 11 cultivars, H. macrophylla ‘Ayesha’ and two hybrids, ‘Sabrina’ and ‘Selina’, were relatively salt-tolerant. H. macrophylla ‘Merritt’s Supreme’ and ‘Mathilda’ were moderately tolerant. H. paniculata ‘Bulk’ was the most sensitive, followed by H. paniculata ‘Interhydia’, and then by H. serrata ‘Preciosa’ and H. macrophylla ‘Passion’, as evidenced by high mortality and severe salt damage symptoms. H. quercifolia ‘Snowflake’ and H. macrophylla ‘Emotion’ were moderately salt-sensitive.

HortScience ◽  
2019 ◽  
Vol 54 (11) ◽  
pp. 2056-2062 ◽  
Author(s):  
Asmita Paudel ◽  
Ji Jhong Chen ◽  
Youping Sun ◽  
Yuxiang Wang ◽  
Richard Anderson

Sego SupremeTM is a designated plant breeding and introduction program at the Utah State University Botanical Center and the Center for Water Efficient Landscaping. This plant selection program introduces native and adapted plants to the arid West for aesthetic landscaping and water conservation. The plants are evaluated for characteristics such as color, flowering, ease of propagation, market demand, disease/pest resistance, and drought tolerance. However, salt tolerance has not been considered during the evaluation processes. Four Sego SupremeTM plants [Aquilegia barnebyi (oil shale columbine), Clematis fruticosa (Mongolian gold clematis), Epilobium septentrionale (northern willowherb), and Tetraneuris acaulis var. arizonica (Arizona four-nerve daisy)] were evaluated for salt tolerance in a greenhouse. Uniform plants were irrigated weekly with a nutrient solution at an electrical conductivity (EC) of 1.25 dS·m−1 as control or a saline solution at an EC of 2.5, 5.0, 7.5, or 10.0 dS·m−1 for 8 weeks. After 8 weeks of irrigation, A. barnebyi irrigated with saline solution at an EC of 5.0 dS·m−1 had slight foliar salt damage with an average visual score of 3.7 (0 = dead; 5 = excellent), and more than 50% of the plants were dead when irrigated with saline solutions at an EC of 7.5 and 10.0 dS·m−1. However, C. fruticosa, E. septentrionale, and T. acaulis had no or minimal foliar salt damage with visual scores of 4.2, 4.1, and 4.3, respectively, when irrigated with saline solution at an EC of 10.0 dS·m−1. As the salinity levels of treatment solutions increased, plant height, leaf area, and shoot dry weight of C. fruticosa and T. acaulis decreased linearly; plant height of A. barnebyi and E. septentrionale also declined linearly, but their leaf area and shoot dry weight decreased quadratically. Compared with the control, the shoot dry weights of A. barnebyi, C. fruticosa, E. septentrionale, and T. acaulis decreased by 71.3%, 56.3%, 69.7%, and 48.1%, respectively, when irrigated with saline solution at an EC of 10.0 dS·m−1. Aquilegia barnebyi and C. fruticosa did not bloom during the experiment at all treatments. Elevated salinity reduced the number of flowers in E. septentrionale and T. acaulis. Elevated salinity also reduced the number of shoots in all four species. Among the four species, sodium (Na+) and chloride (Cl–) concentration increased the most in A. barnebyi by 53 and 48 times, respectively, when irrigated with saline solution at an EC of 10.0 dS·m−1. In this study, C. fruticosa and T. acaulis had minimal foliar salt damage and less reduction in shoot dry weight, indicating that they are more tolerant to salinity. Epilobium septentrionale was moderately tolerant to saline solution irrigation with less foliar damage, although it had more reduction in shoot dry weight. On the other hand, A. barnebyi was the least tolerant with severe foliar damage, more reduction in shoot dry weight, and a greater concentration of Na+ and Cl–.


2019 ◽  
Vol 29 (3) ◽  
pp. 367-373
Author(s):  
Yuxiang Wang ◽  
Liqin Li ◽  
Youping Sun ◽  
Xin Dai

Spirea (Spiraea sp.) plants are commonly used in landscapes in Utah and the intermountain western United States. The relative salt tolerance of seven japanese spirea (Spiraea japonica) cultivars (Galen, Minspi, NCSX1, NCSX2, SMNSJMFP, Tracy, and Yan) were evaluated in a greenhouse. Plants were irrigated with a nutrient solution with an electrical conductivity (EC) of 1.2 dS·m−1 (control) or saline solutions with an EC of 3.0 or 6.0 dS·m−1 once per week for 8 weeks. At 8 weeks after the initiation of treatment, all japanese spirea cultivars irrigated with saline solution with an EC of 3.0 dS·m−1 still exhibited good or excellent visual quality, with all plants having visual scores of 4 or 5 (0 = dead, 1 = severe foliar salt damage, 2 = moderate foliar salt damage, 3 = slight foliar salt damage, 4 = minimal foliar salt damage, 5 = excellent), except for Tracy and Yan, with only 29% and 64%, respectively, of plants with visual scores less than 3. When irrigated with saline solution with an EC of 6.0 dS·m−1, both ‘Tracy’ and ‘Yan’ plants died, and 75% of ‘NCSX2’ plants died. ‘Minspi’ showed severe foliar salt damage, with 32% of plants having a visual score of 1; 25% of plants died. ‘Galen’ and ‘NCSX1’ had slight-to-moderate foliar salt damage, with 25% and 21%, respectively, of plants with visual scores of 2 or less. However, 64% of ‘SMNSJMFP’ plants had good or excellent visual quality, with visual scores more than 4. Saline irrigation water with an EC of 3.0 dS·m−1 decreased the shoot dry weight of ‘Galen’, ‘Minspi’, ‘SMNSJMFP’, and ‘Yan’ by 27%, 22%, 28%, and 35%, respectively, compared with that of the control. All japanese spirea cultivars had 35% to 56% lower shoot dry weight than the control when they were irrigated with saline irrigation water with an EC of 6.0 dS·m−1. The japanese spirea were moderately sensitive to the salinity levels in this experiment. ‘Galen’ and ‘SMNSJMFP’ japanese spirea exhibited less foliar salt damage and reductions in shoot dry weight and were relatively more tolerant to the increased salinity levels tested in this study than the remaining five cultivars (Minspi, NCSX1, NCSX2, Tracy, and Yan).


HortScience ◽  
2017 ◽  
Vol 52 (12) ◽  
pp. 1810-1815 ◽  
Author(s):  
Lifei Chen ◽  
Youping Sun ◽  
Genhua Niu ◽  
Qiang Liu ◽  
James Altland

Relative salt tolerance of eight Berberis thunbergii (japanese barberry) cultivars (B. thunbergii ‘Celeste’, ‘Kasia’, ‘Maria’, ‘Mini’, and ‘Talago’; B. thunbergii var. atropurpurea ‘Concorde’, ‘Helmond Pillar’, and ‘Rose Glow’) was evaluated in a greenhouse experiment. Plants were irrigated with nutrient solution at an electrical conductivity (EC) of 1.2 dS·m−1 (control) or saline solutions at an EC of 5.0 or 10.0 dS·m−1 (EC 5 or EC 10) once a week for 8 weeks. At 4 weeks after treatment, all barberry cultivars in EC 5 had minimal foliar damage with visual scores of 4 or greater (visual score 0: dead, 5: excellent). At 8 weeks after treatment, in EC 5, ‘Helmond Pillar’, ‘Maria’, ‘Mini’, and ‘Rose Glow’ plants exhibited slight foliar salt damage with an average visual score of 3.5, whereas ‘Celeste’, ‘Concorde’, ‘Kasia’, and ‘Talago’ had minimal foliar salt damage with an averaged visual score of 4.4. However, most barberry plants in EC 10 exhibited severe foliar salt damage 4 weeks after treatment with the exception of ‘Concorde’ and were dead 8 weeks after treatment. Compared with control, at the end of the experiment (8 weeks of treatments), shoot dry weight (DW) of ‘Celeste’, ‘Helmond Pillar’, ‘Maria’, and ‘Rose Glow’ in EC 5 was reduced by 47%, 47%, 50%, and 42%, respectively, whereas shoot DW of ‘Concorde’, ‘Kasia’, ‘Mini’, and ‘Talago’ in EC 5 did not change. In EC 10, shoot DW of ‘Celeste’, ‘Concorde’, ‘Kasia’, and ‘Talago’ was reduced by 75%, 35%, 55%, and 46%, respectively. The averaged sodium (Na) concentration of all barberry cultivars in EC 5 and EC 10 was 34 and 87 times, respectively, higher than the control, whereas leaf chloride (Cl) concentration of all barberry cultivars in EC 5 and EC 10 was 14–60 and 29–106 times, respectively, higher than the control. Growth, visual quality, and performance index (PI) were all negatively correlated with leaf Na and Cl content in all cultivars, suggesting that excessive Na and Cl accumulation in the leaf tissue led to growth reduction, salt damage, and death. In summary, ‘Concorde’, ‘Kasia’, and ‘Talago’ were relatively salt tolerant; ‘Helmond Pillar’, ‘Maria’, ‘Mini’, and ‘Rose Glow’ were relatively salt sensitive; and ‘Celeste’ was in between the two groups. Generally, barberry plants had moderate salt tolerance and can be irrigated with marginal water at an EC of 5 dS·m−1 or lower with slight foliar damage.


HortScience ◽  
2017 ◽  
Vol 52 (12) ◽  
pp. 1816-1822 ◽  
Author(s):  
Qiang Liu ◽  
Youping Sun ◽  
Genhua Niu ◽  
James Altland ◽  
Lifei Chen ◽  
...  

Because of limited supply of high-quality water, alternative water sources have been used for irrigation in water-scarce regions. However, alternative waters usually contain high salt levels, which can cause salt damage on salt-sensitive plants. A greenhouse study was conducted to evaluate the relative salt tolerance of 10 common ornamental taxa to saline water irrigation. The 10 taxa studied were Chaenomeles speciosa ‘Orange Storm’ and ‘Pink Storm’ (Chaenomeles Double Take™); Diervilla rivularis ‘G2X885411’, ‘G2X88544’ (Diervilla Kodiak®, Black, Orange, and Red, respectively), and ‘Smndrsf’; Forsythia ×intermedia ‘Mindor’ (Forsythia Show Off®); Hibiscus syriacus ‘ILVOPS’ (Hibiscus Purple Satin®); Hydrangea macrophylla ‘Smhmtau’ and ‘Smnhmsigma’ (Hydrangea Let’s Dance® Blue Jangles® and Rave, respectively); and Parthenocissus quinquefolia ‘Troki’ (Parthenociss quinquefolia Red Wall®). Plants were irrigated with a nutrient solution at an electrical conductivity (EC) of 1.2 dS·m−1 (control) or saline solutions at EC of 5.0 or 10.0 dS·m−1 (EC 5 or EC 10) eight times on a weekly basis. The results indicated that the 10 ornamental taxa had different morphological and physiological responses to salinity. The C. speciosa and D. rivularis plants in EC 5 had severe salt foliar damage, whereas those in EC 10 were dead. Hibiscus syriacus ‘ILVOPS’ performed well in EC 5 treatment with a shoot dry weight (DW) reduction of 26%, but those in EC 10 had severe foliar salt damage. Hydrangea macrophylla, F. ×intermedia ‘Mindor’ and P. quinquefolia ‘Troki’ were the most salt tolerant with minor foliar salt damage. The two H. macrophylla cultivars had the highest shoot sodium (Na) and chlorine (Cl) concentrations with a visual quality of 3 (scale 0 to 5 with 0 for dead plants and 5 for excellent performance), indicating that H. macrophylla plants adapted to elevated salinity by tolerating high Na and Cl concentrations in leaf tissue. Forsythia ×intermedia ‘Mindor’ and P. quinquefolia ‘Troki’ had relatively low leaf Na and Cl concentration, indicating that both taxa are capable of excluding Na and Cl. Chaenomeles speciosa and D. rivularis were sensitive to salinity with great growth reduction, severe foliar salt damage, and high Na and Cl accumulation in leaf tissue.


HortScience ◽  
2012 ◽  
Vol 47 (11) ◽  
pp. 1653-1657 ◽  
Author(s):  
Genhua Niu ◽  
Pedro Osuna ◽  
Youping Sun ◽  
Denise S. Rodriguez

Ornamental chile peppers are popular bedding plants. As high-quality water supply becomes limited in many parts of the world, alternative waters such as municipal reclaimed water is encouraged to be used for landscape irrigation. The purpose of this study was to assess the relative salt tolerance of 10 cultivars of ornamental chile peppers by irrigating the mature plants with saline solutions and germinating seeds in saline substrate in a greenhouse. In the mature plant salt tolerance experiment, plants were irrigated with nutrient solution (no addition of salts, control) or saline solution at electrical conductance (EC) of 4.1 dS·m−1 or 8.1 dS·m−1 for 8 weeks. Plants in the EC of 4.1 dS·m−1 treatment did not have any foliar salt damage regardless of cultivar. At EC of 8.1 dS·m−1, ‘NuMex Memorial Day’ had the most severe foliar salt damage, whereas ‘NuMex April Fool’s Day’, ‘NuMex Cinco de Mayo’, ‘NuMex Thanksgiving’, and ‘NuMex Twilight’ had little or no foliar damage. Shoot dry weight (DW) reduction at EC of 8.1 dS·m−1 compared with control was smallest in ‘NuMex Thanksgiving’ (15%), whereas ‘NuMex Memorial Day’ had the greatest reduction of 74% followed by ‘NuMex Christmas’ of 61%. The highest shoot DW reduction in ‘NuMex Memorial Day’ coincided with lowest visual score, indicating that this cultivar was the least tolerant to salinity. The leaf Na+ and Cl− concentrations increased dramatically with increasing EC of the irrigation water in all cultivars. The highest Na+ concentration of 10.9 mg·g−1 DW at EC of 8.1 dS·m−1 was observed in ‘NuMex Christmas’. The highest Cl− concentration at EC of 8.1 dS·m−1 was found in ‘NuMex Memorial Day’ with 64.8 mg·g−1 DW, which was four times higher than the control. In the seedling emergence experiment, seeds of the 10 cultivars were germinated in substrate either moistened with reverse osmosis water (EC ≈0) or saline solution at EC of 17.1 dS·m−1. ‘NuMex Christmas’ and ‘NuMex Memorial Day’ had the lowest relative seedling emergence index, indicating that these two cultivars were the least tolerant to salinity during the seedling emergence stage. ‘NuMex Thanksgiving’ and ‘NuMex Cinco de Mayo’ had the highest relative seedling emergence index. Combining the results from both experiments, we concluded that ‘NuMex Cinco de Mayo’ and ‘NuMex Thanksgiving’ were the most tolerant cultivars, whereas ‘NuMex Christmas’ and ‘NuMex Memorial Day’ were the least tolerant ones.


HortScience ◽  
2015 ◽  
Vol 50 (10) ◽  
pp. 1562-1566 ◽  
Author(s):  
Youping Sun ◽  
Genhua Niu ◽  
Christina Perez

Salt tolerance of seven Texas Superstar® perennials [Malvaviscus arboreus var. drummondii (Turk’s cap), Phlox paniculata ‘John Fanick’ (‘John Fanick’ phlox), Phlox paniculata ‘Texas Pink’ (‘Texas Pink’ phlox), Ruellia brittoniana ‘Katie Blue’ (‘Katie Blue’ ruellia), Salvia farinacea ‘Henry Duelberg’ (‘Henry Duelberg’ salvia), Salvia leucantha (mexican bush sage), and Verbena ×hybrida ‘Blue Princess’ (‘Blue Princess’ verbena)] was evaluated in a greenhouse experiment. Plants were irrigated with a nutrient solution at electrical conductivity (EC) of 1.1 dS·m−1 (control) or a salt solution at EC of 5.0 or 10.0 dS·m−1 (EC 5 or EC 10) for 8 weeks. ‘John Fanick’ and ‘Texas Pink’ phlox plants in EC 5 had severe salt foliage damage, while those in EC 10 were died. Mexican bush sage in EC 10 had severe salt foliage damage. Turk’s cap, ‘Katie Blue’ ruellia, ‘Henry Duelberg’ salvia, and ‘Blue Princess’ verbena had minor foliar damage regardless of treatment. EC 5 reduced the shoot dry weight (DW) by 45% in ‘Texas Pink’ phlox and 11% to 18% in ‘Katie Blue’ ruellia, ‘Henry Duelberg’ salvia, and mexican bush sage, but did not impact the shoot DW of Turk’s cap and ‘John Fanick’ phlox. EC 10 further decreased the shoot DW of ‘Katie Blue’ ruellia, ‘Henry Duelberg’ salvia, and mexican bush sage plants by 32%, 29%, and 56%, respectively. EC 5 decreased leaf net photosynthesis (Pn) of ‘Texas Pink’ phlox and mexican bush sage, while EC 10 reduced Pn of all species except ‘Henry Duelberg’ salvia and ‘Blue Princess’ verbena. ‘Katie Blue’ ruellia and ‘Blue Princess’ verbena had relatively lower leaf Na concentration and ‘John Fanick’ phlox, ‘Texas Pink’phlox, and mexican bush sage had higher leaf Cl concentrations. In summary, Turk’s cap, ‘Katie Blue’ ruellia, ‘Henry Duelberg’ salvia, and ‘Blue Princess’ verbena were the most tolerant perennials, and ‘John Fanick’ phlox, ‘Texas Pink’ phlox, and mexican bush sage were the least tolerant to salinity.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 522d-522 ◽  
Author(s):  
J.W. Buxton ◽  
D.L. Ingram ◽  
Wenwei Jia

Geraniums in 15-cm pots were irrigated automatically for 8 weeks with a Controlled Water Table (CWT) irrigation system. Plants were irrigated with a nutrient solution supplied by a capillary mat with one end of the mat suspended in a trough below the bottom of the pot. The nutrient solution remained at a constant level in the trough. Nutrient solution removed from the trough was immediately replaced from a larger reservoir. The vertical distance from the surface of the nutrient solution and the bottom of the pot determined the water/air ratio and water potential in the growing media. Treatments consisted of placing pots at 0, 2, 4, and 6 cm above the nutrient solution. Control plants were irrigated as needed with a trickle irrigation system. Geraniums grown at 0,2 and 4 CWT were ≈25% larger than the control plants and those grown at 6 CWT as measured by dry weight and leaf area. Roots of plants grown at 0 CWT were concentrated in the central area of the root ball; whereas roots of plants in other treatments were located more near the bottom of the pot. Advantages of the CWT system include: Plant controlled automatic irrigation; no run off; optimum water/air ratio.


Helia ◽  
2001 ◽  
Vol 24 (35) ◽  
pp. 135-148
Author(s):  
Mohammed El Midaoui ◽  
Ahmed Talouizte ◽  
Benbella Mohamed ◽  
Serieys Hervé ◽  
Ait Houssa Abdelhadi ◽  
...  

SUMMARYAn experiment has been carried out in order to study the behaviour under mineral deficiency of three sunflower genotypes, a population variety (Oro 9) and two hybrids (Mirasol and Albena). Sunflower seedlings were submitted to five treatments: N deficiency (N0), P deficiency (P0), K deficiency (K0), N and K deficiency (N0K0) and a control. Plants were harvested when they reached 3-4 true pairs of leaves. Growth parameters measured (height, total leaf area, root length, root and shoot dry mater) were all significantly reduced by mineral deficiency. Leaf area was most reduced by N0 (-61%) and P0 (-56%). Total dry matter was most affected by N0 (-63%) and by N0K0 (-66%). Genotype comparisons showed that Oro 9 had the highest shoot dry matter while Albena had the lowest root dry matter. Effect of mineral deficiency on content and partitioning of N, P, K, Ca and Na was significant and varied according to treatments and among plant parts. Shoot dry weight was significantly correlated with root N content (r2=0.81) and root K content (r2=-0.61) for N0 and K0.


1978 ◽  
Vol 91 (1) ◽  
pp. 31-45 ◽  
Author(s):  
I. Pearman ◽  
S. M. Thomas ◽  
G. N. Thorne

SummaryEight amounts of nitrogen ranging from 0 to 210 kg N/ha were applied to two tall and one semi-dwarf variety of winter wheat in the spring of 1975 and 1976. The tall varieties were Cappelle-Desprez and Maris Huntsman; the semi-dwarf variety was Maris Fundin in 1975 and Hobbit in 1976. Interactions between varieties and nitrogen were few and small compared with the main effects. All varieties produced their maximum grain yields with 180 kg N/ha. The yield of the semi-dwarf varieties, but not the others, decreased slightly with more nitrogen.Cappelle-Desprez yielded less grain than the other varieties in both years. In 1975 the yields of Maris Fundin and Maris Huntsman were similar and in 1976 Hobbit yielded more than Maris Huntsman. The varieties had similar numbers of ears at maturity and similar patterns of tillering. The semi-dwarf varieties had most grains per spikelet, and hence grains per ear, and Cappelle-Desprez had least. The semi-dwarf varieties had the smallest grains. The semi-dwarf varieties had less straw than the other varieties and hence the largest ratios of grain to total above-ground dry weight. The decrease in dry weight of stem and leaves between anthesis and maturity was similar for all varieties. In 1975 the efficiency of the top two leaves plus top internode in producing grain was the same for all varieties, but in 1976 Hobbit was more efficient than the other two. There were some small differences between varieties in nutrient uptake that were not related to differences in growth. Maris Fundin tended to have a greater phosphorus and potassium content than the tall varieties. Hobbit contained slightly less nitrogen than the tall varieties at maturity, and had a smaller concentration of nitrogen in the grain.Applying 210 kg N/ha doubled grain yield in 1975. Applying nitrogen resulted in a largeincrease in number of ears and a small increase in number of grains per ear due to the development of more fertile spikelets per ear. Nitrogen decreased dry weight per grain, especially of the semi-dwarf varieties. With extra nitrogen, straw dry weight at maturity, shoot dry weight atanthesis and leaf area were all increased relatively more than grain yield, and stems lost moredry weight between anthesis and maturity than without nitrogen. The year 1976 was exceptionallydry and nitrogen had only small effects in that it affected neither straw dry weight nor numberof ears but slightly increased grain yield by increasing the number of spikelets and number of grains per spikelet. It also increased leaf area proportionately to grain yield. In 1975 nitrogen increased evaporation of water from the crop before anthesis but decreased it after anthesis, even though it continued to increase the extraction of water from below 90 cm.


Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 274
Author(s):  
Ademola Adetunji ◽  
Sershen ◽  
Boby Varghese ◽  
Norman Pammenter

Aged seeds exhibit compromised vigour in terms of germination, seedling emergence and growth, but this can to some extent be alleviated by invigoration treatments before sowing. This study aimed to investigate ageing rates and patterns in cabbage (Brassica oleraceae) and lettuce (Lactuca sativa) seeds and whether the beneficial effects of invigorating aged seeds with exogenous antioxidants translate to enhanced seedling performance. Seeds were artificially aged to 25% viability before soaking in 0.4 mM glycerol, 0.6 mM GSH and 0.2 mM trolox for cabbage, and 0.6 mM glycerol, GSH and trolox for lettuce; deionised water served as a control. After 14 days of sowing, seedling emergence percentage, mean emergence time, mean daily emergence, and time taken to 25% emergence were computed. Seedling vigour index, root and shoot dry weight, root:shoot ratio, leaf area, leaf area ratio, and leaf chlorophyll content were assessed 6 weeks after sowing. Furthermore, the photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), and chlorophyll fluorescence were measured 6 weeks after sowing. Notably, ageing resulted in the loss of seed vigour and viability at higher rates in lettuce than cabbage. Seed pretreatment with glycerol promoted seedling growth in both species and shoot dry weight in lettuce, while glycerol and GSH enhanced Pn, Gs and E in lettuce. Trolox also enhanced Pn and E in lettuce. The beneficial effects of the antioxidant treatments are thought to be associated with the protection of photosystems from oxidative stress and/or stimulation of enzymes involved in photosynthesis, possibly through an enhanced antioxidant defence system during the early development stages when seedlings are particularly vulnerable to stress.


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