scholarly journals Giving New Uses to Glucose Meters: Detection of Prostate Cancer

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 24
Author(s):  
Clara Abardía-Serrano ◽  
Rebeca Miranda-Castro ◽  
Noemí de-los-Santos-Álvarez ◽  
María Jesús Lobo-Castañón
Keyword(s):  
Prostate Cancer ◽  
Alkaline Phosphatase ◽  
Nucleic Acid ◽  
Cancer Patients ◽  
Magnetic Particles ◽  
Personal Glucose Meter ◽  
Reliable Detection ◽  
Glucose Meter ◽  
Enzyme Alkaline Phosphatase

A sandwich genoassay for the detection of PCA3, a nucleic acid biomarker overexpressed in the urine of prostate cancer patients, has been developed by using the enzyme alkaline phosphatase (ALP) as a tracer of the hybrid generated onto the surface of magnetic particles. ALP converts D-glucose-1-phosphate into D-glucose, which is quantified with a personal glucose meter. The resulting methodology allows the reliable detection of PCA3 at low picomolar levels, thus fostering massive screening of prostate cancer.

scholarly journals New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

Sensors ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 5514
Author(s):  
Clara Abardía-Serrano ◽  
Rebeca Miranda-Castro ◽  
Noemí de-los-Santos-Álvarez ◽  
María Jesús Lobo-Castañón
Keyword(s):  
Prostate Cancer ◽  
Cancer Screening ◽  
Nucleic Acid ◽  
Magnetic Beads ◽  
Dynamic Range ◽  
Prostate Cancer Screening ◽  
Biological Fluids ◽  
Quantitative Detection ◽  
Personal Glucose Meter ◽  
Glucose Meter

A personal glucose meter (PGM)-based method for quantitative detection of a urinary nucleic acid biomarker in prostate cancer screening, the so-called PCA3, is reported herein. A sandwich-type genoassay is conducted on magnetic beads to collect the target from the sample by specific hybridization, making the assay appropriate for PCA3 detection in biological fluids. The success of the method hinges on the use of alkaline phosphatase (ALP) to link the amount of nucleic acid biomarker to the generation of glucose. In particular, specifically attached ALP molecules hydrolyze D-glucose-1-phosphate into D-glucose, thus enabling the amplification of the recorded signal on the personal glucose meter. The developed genoassay exhibits good sensitivity (3.3 ± 0.2 mg glucose dL−1 pM−1) for PCA3, with a dynamic range of 5 to 100 pM and a quantification limit of 5 pM. Likewise, it facilitates point-of-care testing of nucleic acid biomarkers by using off-the-shelf PGM instead of complex instrumentation involved in traditional laboratory-based tests.

Using a Personal Glucose Meter and Alkaline Phosphatase for Point-of-Care Quantification of Galactose-1-Phosphate Uridyltransferase in Clinical Galactosemia Diagnosis

2015 ◽  
Vol 10 (10) ◽  
pp. 2221-2227 ◽  
Author(s):  
Jingjing Zhang ◽  
Yu Xiang ◽  
Donna E. Novak ◽  
George E. Hoganson ◽  
Junjie Zhu ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Point Of Care ◽  
Personal Glucose Meter ◽  
Glucose Meter

Digital quantitative detection of serum circulating miRNAs using dual-enhanced magnetobiosensors based on cascaded nucleic acid circuits

2019 ◽  
Vol 55 (91) ◽  
pp. 13733-13736 ◽  
Author(s):  
Chunxue Liu ◽  
Yayun An ◽  
Yuanfu Zhang ◽  
Xia Li ◽  
Qingwang Xue ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Quantitative Detection ◽  
Circulating Mirnas ◽  
Personal Glucose Meter ◽  
Glucose Meter

Here, we developed a dual-enhanced magnetobiosensor based on cascaded nucleic acid circuits for sensitive, portable and digital quantitative detection of circulating miRNAs in serum by a personal glucose meter (PGM).

scholarly journals An exploration of nucleic acid liquid biopsy using a glucose meter

2018 ◽  
Vol 9 (14) ◽  
pp. 3517-3522 ◽  
Author(s):  
Yu Gu ◽  
Ting-Ting Zhang ◽  
Zhi-Feng Huang ◽  
Shan-Wen Hu ◽  
Wei Zhao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Liquid Biopsy ◽  
Proof Of Concept ◽  
Circulating Mirna ◽  
Personal Glucose Meter ◽  
Glucose Meter

A proof-of-concept strategy for a circulating miRNA assay using a personal glucose meter (PGM) was proposed.

Detection of lymph node metastases using pooling method by One-Step Nucleic Acid Amplification (OSNA) assay in prostate cancer patients: Preliminary results

2021 ◽  
Vol 79 ◽  
pp. S1291-S1292
Author(s):  
M. Cuadras Sole ◽  
J. Planas Morin ◽  
A. Celma Domènech ◽  
L. Regis Plácido ◽  
M.E. Semidey Raven ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Lymph Node ◽  
Nucleic Acid ◽  
Cancer Patients ◽  
Lymph Node Metastases ◽  
Nucleic Acid Amplification ◽  
Preliminary Results ◽  
One Step ◽  
Node Metastases ◽  
Osna Assay
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