Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea

Currently, due to abuse in the use of human antibiotics and the weak regulatory control that the authorities have over sewage discharge and manure management, antibiotic resistance genes (ARGs) have become a new type of environmental pollutant. Three different natural water bodies (Poyang Lake, Haihe River and Qingdao No.1 Bathing Beach seawater) were sampled during the same periods to conduct a longitudinal comparison of distribution. The distribution and expression of 11 ARGs in 20 species were studied, and the correlations between the expression and the distribution of time and space of the ARGs in different water bodies were also analyzed. With the exception of ermA, blaNDM-1 and vanA, which were not detected in seawater, the other ARGs could be detected in all three water bodies. Tetracycline resistance genes (tetC, tetM and tetQ) in the seawater and Haihe River had even reached 100%, and sulfa ARGs (sul1 and sul2) in the seawater and Poyang Lake, as well as sul2 and sul3 in the Haihe River, had also reached 100%. The ARG pollution in Haihe River was much more serious, since 14 and 17 of 20 ARG species were significantly higher compared with seawater and Poyang Lake, respectively. Some ARGs also had a high absolute abundance. The absolute abundance of macrolide resistance genes (ermB) in seawater was as high as 8.61 × 107 copies/L, and the anti-tuberculosis resistant genes (rpoB and katG) in the Haihe River Basin were highly abundant at 1.32 × 106 copies/L and 1.06 × 107 copies/L, respectively. This indicates that ARGs have gradually become more diverse and extensive in natural water bodies. The results of a redundancy analysis (RDA) of the three water bodies showed that although each water body is affected by different factors in space and time, overall, the presence of AGRs is closely related to the production and life of human beings and the migration of animals.

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Characterization of antibiotic resistance genes and bacterial community in selected municipal and industrial sewage treatment plants beside Poyang Lake

Water Research ◽

10.1016/j.watres.2020.115603 ◽

2020 ◽

Vol 174 ◽

pp. 115603 ◽

Cited By ~ 4

Author(s):

Huijun Ding ◽

Min Qiao ◽

Jiayou Zhong ◽

Yongguan Zhu ◽

Chunjing Guo ◽

...

Keyword(s):

Antibiotic Resistance ◽

Bacterial Community ◽

Resistance Genes ◽

Poyang Lake ◽

Sewage Treatment ◽

Antibiotic Resistance Genes ◽

Sewage Treatment Plants ◽

Industrial Sewage

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Conjugative multi-resistant plasmids in Haihe River and their impacts on the abundance and spatial distribution of antibiotic resistance genes

Water Research ◽

10.1016/j.watres.2016.12.046 ◽

2017 ◽

Vol 111 ◽

pp. 81-91 ◽

Cited By ~ 53

Author(s):

Bingjun Dang ◽

Daqing Mao ◽

Yan Xu ◽

Yi Luo

Keyword(s):

Antibiotic Resistance ◽

Spatial Distribution ◽

Resistance Genes ◽

Antibiotic Resistance Genes ◽

Haihe River

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Prevalence of sulfonamide-resistant bacteria, resistance genes and integron-associated horizontal gene transfer in natural water bodies and soils adjacent to a swine feedlot in northern Taiwan

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2014.02.016 ◽

2014 ◽

Vol 277 ◽

pp. 34-43 ◽

Cited By ~ 79

Author(s):

Jih-Tay Hsu ◽

Chia-Yang Chen ◽

Chu-Wen Young ◽

Wei-Liang Chao ◽

Mao-Hao Li ◽

...

Keyword(s):

Gene Transfer ◽

Horizontal Gene Transfer ◽

Resistance Genes ◽

Natural Water ◽

Water Bodies ◽

Resistant Bacteria ◽

Bacteria Resistance ◽

Natural Water Bodies ◽

Northern Taiwan

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Trends in Antibiotic Resistance Genes Occurrence in the Haihe River, China

Environmental Science & Technology ◽

10.1021/es100233w ◽

2010 ◽

Vol 44 (19) ◽

pp. 7220-7225 ◽

Cited By ~ 432

Author(s):

Yi Luo ◽

Daqing Mao ◽

Michal Rysz ◽

Qixing Zhou ◽

Hongjie Zhang ◽

...

Keyword(s):

Antibiotic Resistance ◽

Resistance Genes ◽

Antibiotic Resistance Genes ◽

Haihe River

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Taxonomic and Functional Distribution of Bacterial Communities in Domestic and Hospital Wastewater System: Implications for Public and Environmental Health

Antibiotics ◽

10.3390/antibiotics10091059 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1059

Author(s):

Ramganesh Selvarajan ◽

Timothy Sibanda ◽

Jeevan Pandian ◽

Kevin Mearns

Keyword(s):

Antibiotic Resistance ◽

Environmental Health ◽

Resistance Genes ◽

Bacterial Communities ◽

Antibiotic Resistance Genes ◽

Domestic Wastewater ◽

Water Bodies ◽

Resistant Bacteria ◽

Hospital Wastewater ◽

Sequence Coverage

The discharge of untreated hospital and domestic wastewater into receiving water bodies is still a prevalent practice in developing countries. Unfortunately, because of an ever-increasing population of people who are perennially under medication, these wastewaters contain residues of antibiotics and other antimicrobials as well as microbial shedding, the direct and indirect effects of which include the dissemination of antibiotic resistance genes and an increase in the evolution of antibiotic-resistant bacteria that pose a threat to public and environmental health. This study assessed the taxonomic and functional profiles of bacterial communities, as well as the antibiotic concentrations in untreated domestic wastewater (DWW) and hospital wastewater (HWW), using high-throughput sequencing analysis and solid-phase extraction coupled to Ultra-high-performance liquid chromatography Mass Spectrometry (UHPLC–MS/MS) analysis, respectively. The physicochemical qualities of both wastewater systems were also determined. The mean concentration of antibiotics and the concentrations of Cl−, F− and PO43 were higher in HWW samples than in DWW samples. The phylum Firmicutes was dominant in DWW with a sequence coverage of 59.61% while Proteobacteria was dominant in HWW samples with a sequence coverage of 86.32%. At genus level, the genus Exiguobacterium (20.65%) and Roseomonas (67.41%) were predominant in DWW and HWW samples, respectively. Several pathogenic or opportunistic bacterial genera were detected in HWW (Enterococcus, Pseudomonas and Vibrio) and DWW (Clostridium, Klebsiella, Corynebacterium, Bordetella, Staphylocccus and Rhodococcus) samples. Functional prediction analysis indicated the presence of beta-lactam resistance, cationic antimicrobial peptide (CAMP) resistance and vancomycin resistance genes in HWW samples. The presence of these antibiotic resistance genes and cassettes were positively correlated with the presence of pathogens. These findings show the risk posed to public and environmental health by the discharge of untreated domestic and hospital wastewaters into environmental water bodies.

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New Estimation of Antibiotic Resistance Genes in Sediment Along the Haihe River and Bohai Bay in China: A Comparison Between Single and Successive DNA Extraction Methods

Frontiers in Microbiology ◽

10.3389/fmicb.2021.705724 ◽

2021 ◽

Vol 12 ◽

Author(s):

Chao Wu ◽

Guicheng Zhang ◽

Wenzhe Xu ◽

Shan Jian ◽

Liyin Peng ◽

...

Keyword(s):

Antibiotic Resistance ◽

Dna Extraction ◽

Resistance Genes ◽

Antibiotic Resistance Genes ◽

Rrna Gene ◽

Bohai Bay ◽

Haihe River ◽

Study Region ◽

Sediment Samples ◽

Successive Extraction

Sediment is thought to be a vital reservoir for antibiotic resistance genes (ARGs). Often, studies describing and comparing ARGs and their potential hosts in sediment are based on single DNA extractions. To date, however, no study has been conducted to assess the influence of DNA extraction efficiency on ARGs in sediment. To determine whether the abundance of ARGs is underestimated, we performed five successive extraction cycles with a widely used commercial kit in 10 sediment samples collected from the Haihe River and Bohai Bay. Our results showed that accumulated DNA yields after five extractions were 1.8–3.1 times higher than that by single DNA extractions. High-throughput sequencing showed that insufficient DNA extraction could generate PCR bias and skew community structure characterization in sediment. The relative abundances of some pathogenic bacteria, such as Enterobacteriales, Lactobacillales, and Streptomycetales, were significantly different between single and successive DNA extraction samples. In addition, real-time fluorescent quantitative PCR (qPCR) showed that ARGs, intI1, and 16S rRNA gene abundance strongly increased with increasing extraction cycles. Among the measured ARGs, sulfonamide resistance genes and multidrug resistance genes were dominant subtypes in the study region. Nevertheless, different subtypes of ARGs did not respond equally to the additional extraction cycles; some continued to have linear growth trends, and some tended to level off. Additionally, more correlations between ARGs and bacterial communities were observed in the successive DNA extraction samples than in the single DNA extraction samples. It is suggested that 3–4 additional extraction cycles are required in future studies when extracting DNA from sediment samples. Taken together, our results highlight that performing successive DNA extractions on sediment samples optimizes the extractable DNA yield and can lead to a better picture of the abundance of ARGs and their potential hosts in sediments.

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Metagenomic Analysis of Bacterial Communities and Antibiotic Resistance Genes in Penaeus monodon Biofloc-Based Aquaculture Environments

Frontiers in Marine Science ◽

10.3389/fmars.2021.762345 ◽

2022 ◽

Vol 8 ◽

Author(s):

Xieyan Chen ◽

Zihao He ◽

Jichen Zhao ◽

Minze Liao ◽

Yuan Xue ◽

...

Keyword(s):

Antibiotic Resistance ◽

Resistance Genes ◽

Bacterial Communities ◽

High Throughput Sequencing ◽

Waste Treatment ◽

Gene Annotation ◽

Antibiotic Resistance Gene ◽

Penaeus Monodon ◽

Antibiotic Resistance Genes ◽

Absolute Abundance

Biofloc technology (BFT) is one of the most promising technologies in global aquaculture for the purpose of improving water quality, waste treatment, and disease prevention in intensive aquaculture systems. However, characterization of the microbial species and antibiotic resistance potentially present in biofloc-based aquaculture environments is needed. In this study, we used high-throughput sequencing technology to comprehensively compare the bacterial communities in mariculture ponds of Penaeus monodon (P. monodon), by testing of water, biofloc, and intestine of P. monodon. Operational taxonomic units (OTUs) cluster analysis showed that the nine samples tested divided into 45 phyla and 457 genera. Proteobacteria was the dominant bacteria in water, biofloc and prawn intestine. In biofloc and intestine, the Ruegeria (2.23–6.31%) genus represented the largest proportion of bacteria, with Marivita (14.01–20.94%) the largest group in water. Microbial functional annotation revealed that in all the samples, genes encoding metabolism were predominant. The antibiotic resistance gene annotation showed the highest absolute abundance of patB, adeF, OXA-243, and Brucella_suis_mprF from Proteobacteria. PatB (11.33–15.01%), adeF (15.79–18.16%), OXA-243 (35.65%), and Brucella_suis_mprF (10.03%) showed the highest absolute abundance of antibiotic resistance genes in water, biofloc, and intestines, respectively. These findings may greatly increase our understanding of the characteristics of the microbiota of shrimp biofloc-based aquaculture systems and the complex interactions among shrimp, ambient microflora, and environmental variables. It provides a reference basis for policy on breeding, environmental safety, and maintaining food safety in the production of P. monodon.

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Fate of antibiotic resistant E. coli and antibiotic resistance genes during full scale conventional and advanced anaerobic digestion of sewage sludge

PLoS ONE ◽

10.1371/journal.pone.0237283 ◽

2020 ◽

Vol 15 (12) ◽

pp. e0237283

Author(s):

Sky Redhead ◽

Jeroen Nieuwland ◽

Sandra Esteves ◽

Do-Hoon Lee ◽

Dae-Wi Kim ◽

...

Keyword(s):

Antibiotic Resistance ◽

Anaerobic Digestion ◽

Sewage Sludge ◽

Relative Abundance ◽

Resistance Genes ◽

Antibiotic Resistance Genes ◽

Absolute Abundance ◽

Sludge Treatment ◽

Antibiotic Resistant ◽

Pre Treatment

Antibiotic resistant bacteria (ARB) and their genes (ARGs) have become recognised as significant emerging environmental pollutants. ARB and ARGs in sewage sludge can be transmitted back to humans via the food chain when sludge is recycled to agricultural land, making sludge treatment key to control the release of ARB and ARGs to the environment. This study investigated the fate of antibiotic resistant Escherichia coli and a large set of antibiotic resistance genes (ARGs) during full scale anaerobic digestion (AD) of sewage sludge at two U.K. wastewater treatment plants and evaluated the impact of thermal hydrolysis (TH) pre-treatment on their abundance and diversity. Absolute abundance of 13 ARGs and the Class I integron gene intI1 was calculated using single gene quantitative (q) PCR. High through-put qPCR analysis was also used to determine the relative abundance of 370 ARGs and mobile genetic elements (MGEs). Results revealed that TH reduced the absolute abundance of all ARGs tested and intI1 by 10–12,000 fold. After subsequent AD, a rebound effect was seen in many ARGs. The fate of ARGs during AD without pre-treatment was variable. Relative abundance of most ARGs and MGEs decreased or fluctuated, with the exception of macrolide resistance genes, which were enriched at both plants, and tetracyline and glycopeptide resistance genes which were enriched in the plant employing TH. Diversity of ARGs and MGEs decreased in both plants during sludge treatment. Principal coordinates analysis revealed that ARGs are clearly distinguished according to treatment step, whereas MGEs in digested sludge cluster according to site. This study provides a comprehensive within-digestor analysis of the fate of ARGs, MGEs and antibiotic resistant E. coli and highlights the effectiveness of AD, particularly when TH is used as a pre-treatment, at reducing the abundance of most ARGs and MGEs in sludgeand preventing their release into the environment.

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Antibiotic Resistance Genes from Mat and Planktonic Microbiomes of Antarctic Lake Fryxell and Lake Bonney, and a Mat-forming Culture of Rhodoferax antarcticus

10.1101/2021.12.17.473166 ◽

2021 ◽

Author(s):

Sheetal Tallada ◽

Grant Hall ◽

Daniel Barich ◽

Joan L Slonczewski

Keyword(s):

Antibiotic Resistance ◽

Resistance Genes ◽

Water Samples ◽

Antibiotic Resistance Genes ◽

Water Bodies ◽

Antarctic Lake ◽

Lake Fryxell ◽

Rhodoferax Antarcticus ◽

Lift Off ◽

The Antarctic

The Antarctic Taylor Valley Lakes Fryxell and Bonney harbor oligotrophic microbial communities that are separated geographically from other aquatic systems. Their microbiomes include planktonic as well as lift-off mat communities that float to the underside of the perennial ice cover and eventually emerge at the surface. We investigated the antibiotic resistance genes (ARGs) from metagenomes of lift-off mats emerging from ice, from filtered water samples of Lake Fryxell, and from filtered water samples of Lake Bonney. ARG sequence markers were designed by ShortBRED-Identify using the Comprehensive Antibiotic Resistance Database (CARD). The overall proportion of ARG hits in the metagenomes was found to be similar to that found in temperate-zone rural water bodies with moderate human inputs (0.0002-0.0007%). The specific ARGs found showed distinct distributions for the two lakes, and for mat versus planktonic sources. An enrichment culture of Rhodoferax antarcticus from a Lake Fryxell mat sample showed a mat-forming phenotype not previously reported for this species. Its genome showed no ARGs associated with Betaproteobacteria, but had ARGs consistent with a Pseudomonas minor component. The Antarctic lake mats and water showed specific ARGs distinctive to the mat and water sources, but overall ARG levels were similar to those of temperate water bodies.

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