scholarly journals Evaluation of an Environmental Transport Medium for Legionella pneumophila Recovery

2021 ◽  
Vol 18 (16) ◽  
pp. 8551
Author(s):  
Marianna Martinelli ◽  
Enrico Calaresu ◽  
Rosario Musumeci ◽  
Chiara Giubbi ◽  
Federica Perdoni ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Room Temperature ◽  
Storage Conditions ◽  
Transport Medium ◽  
Molecular Tests ◽  
Environmental Transport ◽  
Culture Viability ◽  
Neutralizing Agent ◽  
The Stability ◽  
And Storage

The collection and storage of water-related matrices such as biofilm from collection to processing are critical for the detection of Legionella pneumophila by cultural and molecular tests. SRK™ is a liquid medium that acts both as an antimicrobial neutralizing agent and a transport medium for bacterial culture enumeration and is useful to maintain the stability of the sample from collection to analysis. The aims of this study were to evaluate Legionella pneumophila viability and bacterial nucleic acids’ stability in SRK™ medium over time at different storage conditions. Artificial bacterial inoculates with an approximate concentration of 104, 103 and 102 CFU/mL were made using Legionella pneumophila certified reference material suspended in SRK™ medium. Bacteria recovery was analyzed by cultural and molecular methods at time 0, 24 and 48 h at room temperature and at 0, 24, 48 and 72 h at 2–8 °C, respectively. SRK™ medium supported Legionella pneumophila culture viability with CFU counts within the expected range. The recovery after 72 h at 2–8 °C was 83–100% and 75–95% after 48 h at room temperature. Real-time PCR appropriately detected Legionella pneumophila DNA at each temperature condition, dilution and time point. Results demonstrated a good performance of SRK™ medium for the reliable recovery of environmental Legionella.

Human plasma stability during handling and storage: impact on NMR metabolomics

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 1168-1177 ◽  
Author(s):  
Joana Pinto ◽  
M. Rosário M. Domingues ◽  
Eulália Galhano ◽  
Cristina Pita ◽  
Maria do Céu Almeida ◽  
...  
Keyword(s):  
Human Plasma ◽  
Room Temperature ◽  
Storage Conditions ◽  
Plasma Composition ◽  
Long Term Storage ◽  
Short And Long Term ◽  
The Stability ◽  
And Storage ◽  
Term Storage

The stability of human plasma composition was investigated by NMR, considering different collection tubes, time at room temperature (RT), short- and long-term storage conditions and up to 5 consecutive freeze–thaw cycles.

Effect of pH and temperature on the infectivity of human coronavirus 229E

1989 ◽  
Vol 35 (10) ◽  
pp. 972-974 ◽  
Author(s):  
Alain Lamarre ◽  
Pierre J. Talbot
Keyword(s):  
Incubation Period ◽  
Storage Conditions ◽  
Viral Infectivity ◽  
Human Coronavirus ◽  
Virus Growth ◽  
The Stability ◽  
And Storage ◽  
Influence Of Ph ◽  
Human Coronavirus 229E

The stability of human coronavirus 229E infectivity was maximum at pH 6.0 when incubated at either 4 or 33 °C. However, the influence of pH was more pronounced at 33 °C. Viral infectivity was completely lost after a 14-day incubation period at 22, 33, or 37 °C but remained relatively constant at 4 °C for the same length of time. Finally, the infectious titer did not show any significant reduction when subjected to 25 cycles of thawing and freezing. These studies will contribute to optimize virus growth and storage conditions, which will facilitate the molecular characterization of this important pathogen.Key words: coronavirus, pH, temperature, infectivity, human coronavirus.

scholarly journals Pre-Drawn Syringes of Comirnaty for an Efficient COVID-19 Mass Vaccination: Demonstration of Stability

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1029
Author(s):  
Francesca Selmin ◽  
Umberto M. Musazzi ◽  
Silvia Franzè ◽  
Edoardo Scarpa ◽  
Loris Rizzello ◽  
...  
Keyword(s):  
Mechanical Stress ◽  
Real Life ◽  
Storage Conditions ◽  
Lipid Nanoparticles ◽  
Mass Vaccination ◽  
Hydrodynamic Diameter ◽  
The Us ◽  
Real Mass ◽  
The Stability ◽  
And Storage

Moving towards a real mass vaccination in the context of COVID-19, healthcare professionals are required to face some criticisms due to limited data on the stability of a mRNA-based vaccine (Pfizer-BioNTech COVID-19 Vaccine in the US or Comirnaty in EU) as a dose in a 1 mL-syringe. The stability of the lipid nanoparticles and the encapsulated mRNA was evaluated in a “real-life” scenario. Specifically, we investigated the effects of different storing materials (e.g., syringes vs. glass vials), as well as of temperature and mechanical stress on nucleic acid integrity, number, and particle size distribution of lipid nanoparticles. After 5 h in the syringe, lipid nanoparticles maintained the regular round shape, and the hydrodynamic diameter ranged between 80 and 100 nm with a relatively narrow polydispersity (<0.2). Samples were stable independently of syringe materials and storage conditions. Only strong mechanical stress (e.g., shaking) caused massive aggregation of lipid nanoparticles and mRNA degradation. These proof-of-concept experiments support the hypothesis that vaccine doses can be safely prepared in a dedicated area using an aseptic technique and transferred without affecting their stability.

Stability of extemporaneously prepared rosuvastatin oral suspension

2017 ◽  
Vol 74 (19) ◽  
pp. 1579-1583 ◽  
Author(s):  
Abdel Naser Zaid ◽  
Rania Shtayah ◽  
Ayman Qadumi ◽  
Mashour Ghanem ◽  
Rawan Qedan ◽  
...  
Keyword(s):  
Relative Humidity ◽  
High Performance ◽  
Room Temperature ◽  
Microbial Contamination ◽  
Active Pharmaceutical Ingredient ◽  
Storage Conditions ◽  
Dissolution Profile ◽  
Stability Studies ◽  
Organoleptic Properties ◽  
The Stability

Abstract Purpose The stability of an extemporaneously prepared rosuvastatin suspension stored over 30 days under various storage conditions was evaluated. Methods Rosuvastatin suspension was extemporaneously prepared using commercial rosuvastatin tablets as the source of active pharmaceutical ingredient. The organoleptic properties, dissolution profile, and stability of the formulation were investigated. For the stability studies, samples of the suspension were stored under 2 storage conditions, room temperature (25 °C and 60% relative humidity) and accelerated stability chambers (40 °C and 75% relative humidity). Viscosity, pH, organoleptic properties, and microbial contamination were evaluated according to the approved specifications. High-performance liquid chromatography was used for the analysis and quantification of rosuvastatin in selected samples. Microbiological investigations were also conducted. Results The prepared suspension showed acceptable organoleptic properties. It showed complete release of rosuvastatin within 15 minutes. The pH of the suspension was 9.8, which remained unchanged during the stability studies. The microbiological investigations demonstrated that the preparation was free of any microbial contamination. In addition, the suspension showed stability within at least the period of use of a 100-mL rosuvastatin bottle. Conclusion Extemporaneously prepared rosuvastatin 20-mg/mL suspension was stable for 30 days when stored at room temperature.

The stability of acid-soluble chromosomal proteins from avian erythrocytes

1968 ◽  
Vol 46 (8) ◽  
pp. 781-788 ◽  
Author(s):  
G. Vidali ◽  
J. M. Neelin
Keyword(s):  
Acetic Acid ◽  
Room Temperature ◽  
Starch Gel Electrophoresis ◽  
Guanidinium Chloride ◽  
Chromosomal Proteins ◽  
Avian Erythrocytes ◽  
Starch Gel ◽  
Avian Erythrocyte ◽  
The Stability ◽  
And Storage

The stability of avian erythrocyte histones was examined under the conditions of extraction, chromatography, electrophoresis, and storage, in order to avoid degradation during these operations. Since turbidity in trichloroacetic acid (TCA) was used as a measure of histone integrity, optimal conditions for quantitative assay were established as follows. One volume of histone sample was mixed with five volumes of 1.1 M TCA at room temperature, and the optical density at 400 mμ was measured after 25 min. The relation between turbidity and protein concentration was linear from 0.03 to at least 0.3 mg histone per milliliter and was not related to the kind of histone, except for the lysine-rich fraction which showed slightly less specific turbidity. Under these conditions turbidity was not sensitive to concomitant solutes such as guanidinium chloride, acetic acid, and dilute buffers and acids.With turbidity as the criterion of protein integrity, it was confirmed that brief manipulation in the cold is desirable in most media, including the dilute acids used for extraction. Nevertheless, chromatography at room temperature in concentrated solutions of guanidinium chloride or acetic acid appears to be tolerably safe. The effect of these conditions of manipulation and storage on histone fractions was substantiated by chromatography and starch-gel electrophoresis. Prolonged extraction of avian erythrocyte nuclei at acid pH released additional non-histone basic protein without alteration of authentic histone fractions.

scholarly journals An Assessment of Mesoporous Silica Nanoparticle Architectures as Antigen Carriers

Pharmaceutics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 294 ◽  
Author(s):  
Xinyue Huang ◽  
Helen E Townley
Keyword(s):  
Mesoporous Silica ◽  
Mesoporous Silica Nanoparticles ◽  
High Surface ◽  
Silica Nanoparticle ◽  
Storage Conditions ◽  
Surface Areas ◽  
Antigen Complex ◽  
The Stability ◽  
And Storage ◽  
Storage Temperatures

Mesoporous silica nanoparticles (MSNPs) have the potential to be used as antigen carriers due to their high surface areas and highly ordered pore network. We investigated the adsorption and desorption of diphtheria toxoid as a proof-of-concept. Two series of nanoparticles were prepared—(i) small pores (SP) (<10 nm) and (ii) large pores (LP) (>10 nm). SBA-15 was included as a comparison since this is commercially available and has been used in a large number of studies. External diameters of the particles ranged from 138 to 1509 nm, surface area from 632 to 1110 m2/g and pore size from 2.59 to 16.48 nm. Antigen loading was assessed at a number of different ratios of silica-to-antigen and at 4 °C, 20 °C and 37 °C. Our data showed that protein adsorption by the SP series was in general consistently lower than that shown by the large pore series. Unloading was then examined at 4 °C, 20 °C and 37 °C and a pH 1.2, 4.5, 6.8 and 7.4. There was a trend amongst the LP particles towards the smallest pores showing the lowest release of antigen. The stability of the MSNP: antigen complex was tested at two different storage temperatures, and storage in solution or after lyophilization. After 6 months there was negligible release from any of the particles under any of the storage conditions. The particles were also shown not to cause hemolysis.

scholarly journals Effects of Storage Conditions on Postharvest Qualities in Garlic Bulbs (Allium sativum L.)

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 805C-805
Author(s):  
Sun-Tay Choi ◽  
Ro-Na Bae* ◽  
Dae-Sung Chung ◽  
Seung-Koo Lee
Keyword(s):  
Weight Loss ◽  
Low Temperature ◽  
Pyruvic Acid ◽  
Room Temperature ◽  
Allium Sativum ◽  
Storage Period ◽  
Storage Conditions ◽  
Controlled Atmosphere ◽  
Atmosphere Storage ◽  
And Storage

To investigate quality changes of garlic associated with cultivars and storage conditions, northern type `Seosan' and sub-tropical type `Daeseo' garlics were stored at controlled atmosphere (O2 3%, CO2 5%, -1 ± 1°C) condition, low temperature (-1 ± 1°C), and room temperature (20 ± 5°C). The rate of sprouting, weight loss, enzymatic pyruvic acid content, and degree of greening in crushed garlic were determined during storage. The rate of sprouting was higher in `Daeseo' than in `Seosan' garlic in all storage conditions. Sprouting was effectively suppressed in low temperature and controlled atmosphere storage. Weight loss in `Daeseo' garlic was higher than in `Seosan' garlic. Enzymatic pyruvic acid (EP) contents increased for 3 months storage period, and then decreased gradually as the storage period was prolonged at room or low temperatures. However, EP content decreased dramatically during storage under CA condition in both cultivars. When garlic bulbs were crushed, greening appeared in the garlic stored at low temperature for more than one month. However, greening did not occur in the crushed garlic bulbs stored in CA condition.

scholarly journals Shelf Life and Storage Conditions of Universal Adhesives: A Literature Review

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2708
Author(s):  
Georgi Iliev ◽  
Louis Hardan ◽  
Cynthia Kassis ◽  
Rim Bourgi ◽  
Carlos Enrique Cuevas-Suárez ◽  
...  
Keyword(s):  
Shelf Life ◽  
Clinical Importance ◽  
Storage Conditions ◽  
Adhesive Systems ◽  
Hybrid Layer ◽  
Universal Adhesive ◽  
Bond Performance ◽  
Universal Adhesives ◽  
The Stability ◽  
And Storage

This paper presents state of the art universal adhesive systems and the effect of shelf-life and storage conditions on their bond performance. Three topics are explored in this review: an introduction to the topic, the mechanisms responsible for the degradation of the hybrid layer, and the factors that play a role in the stability of universal adhesives. In addition, issues such as potential durability and clinical importance are discussed. Universal adhesive systems are promising but must be handled and stored according to the manufacturer’s instructions, with careful attention given to the details of shelf-life and storage conditions for maximal success. It appears that the components of universal adhesives play an important role in their stability. Furthermore, HEMA-free formulations using methacrylamides lead to longer shelf-life. Further research is needed to prove these hypotheses.

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