Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions

Improving soybean growth and tolerance under environmental stress is crucial for sustainable development. Millimeter waves are a radio-frequency band with a wavelength range of 1–10 mm that has dynamic effects on organisms. To investigate the potential effects of millimeter-waves irradiation on soybean seedlings, morphological and proteomic analyses were performed. Millimeter-waves irradiation improved the growth of roots/hypocotyl and the tolerance of soybean to flooding stress. Proteomic analysis indicated that the irradiated soybean seedlings recovered under oxidative stress during growth, whereas proteins related to glycolysis and ascorbate/glutathione metabolism were not affected. Immunoblot analysis confirmed the promotive effect of millimeter waves to glycolysis- and redox-related pathways under flooding conditions. Sugar metabolism was suppressed under flooding in unirradiated soybean seedlings, whereas it was activated in the irradiated ones, especially trehalose synthesis. These results suggest that millimeter-waves irradiation on soybean seeds promotes the recovery of soybean seedlings under oxidative stress, which positively regulates soybean growth through the regulation of glycolysis and redox related pathways.

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1083 Proteomic analysis reveals increased Nrf2-mediated oxidative stress response in adipose tissue of late pregnant dairy cows during summer heat stress

Journal of Animal Science ◽

10.2527/jam2016-1083 ◽

2016 ◽

Vol 94 (suppl_5) ◽

pp. 519-520

Author(s):

M. Zachut ◽

G. Kra ◽

G. Friedlander ◽

Y. Levin

Keyword(s):

Oxidative Stress ◽

Adipose Tissue ◽

Heat Stress ◽

Stress Response ◽

Dairy Cows ◽

Proteomic Analysis ◽

Oxidative Stress Response ◽

Summer Heat

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Metabolic Profiling Analysis Reveals the Potential Contribution of Barley Sprouts against Oxidative Stress and Related Liver Cell Damage in Habitual Alcohol Drinkers

Antioxidants ◽

10.3390/antiox10030459 ◽

2021 ◽

Vol 10 (3) ◽

pp. 459

Author(s):

Hyerin Park ◽

Eunok Lee ◽

Yunsoo Kim ◽

Hye Yoon Jung ◽

Kwang-Min Kim ◽

...

Keyword(s):

Oxidative Stress ◽

Fatty Liver ◽

Molecular Mechanisms ◽

Learning Algorithm ◽

Controlled Trial ◽

Liver Fat ◽

Glutathione Metabolism ◽

Ros Generation ◽

Potential Contribution ◽

Glutamyl Transferase

Chronic excessive alcohol consumption is associated with multiple liver defects, such as steatosis and cirrhosis, mainly attributable to excessive reactive oxygen species (ROS) production. Barley sprouts (Hordeum vulgare L.) contain high levels of polyphenols that may serve as potential antioxidants. This study aimed to investigate whether barley sprouts extract powder (BSE) relieves alcohol-induced oxidative stress and related hepatic damages in habitual alcohol drinkers with fatty liver. In a 12-week randomized controlled trial with two arms (placebo or 480 mg/day BSE; n = 76), we measured clinical markers and metabolites at the baseline and endpoint to understand the complex molecular mechanisms. BSE supplementation reduced the magnitude of ROS generation and lipid peroxidation and improved the glutathione antioxidant system. Subsequent metabolomic analysis identified alterations in glutathione metabolism, amino acid metabolism, and fatty acid synthesis pathways, confirming the role of BSE in glutathione-related lipid metabolism. Finally, the unsupervised machine learning algorithm indicated that subjects with lower glutathione reductase at the baseline were responders for liver fat content, and those with higher fatigue and lipid oxidation were responders for γ-glutamyl transferase. These findings suggest that BSE administration may protect against hepatic injury by reducing oxidative stress and changing the metabolism in habitual alcohol drinkers with fatty liver.

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Oxidative Stress, Glutathione Metabolism, and Liver Regeneration Pathways Are Activated in Hereditary Tyrosinemia Type 1 Mice upon Short-Term Nitisinone Discontinuation

Genes ◽

10.3390/genes12010003 ◽

2020 ◽

Vol 12 (1) ◽

pp. 3

Author(s):

Haaike Colemonts-Vroninks ◽

Jessie Neuckermans ◽

Lionel Marcelis ◽

Paul Claes ◽

Steven Branson ◽

...

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Liver Regeneration ◽

The Body ◽

Glutathione Metabolism ◽

Short Term ◽

Therapeutic Regime ◽

Tyrosinemia Type 1 ◽

Hereditary Tyrosinemia

Hereditary tyrosinemia type 1 (HT1) is an inherited condition in which the body is unable to break down the amino acid tyrosine due to mutations in the fumarylacetoacetate hydrolase (FAH) gene, coding for the final enzyme of the tyrosine degradation pathway. As a consequence, HT1 patients accumulate toxic tyrosine derivatives causing severe liver damage. Since its introduction, the drug nitisinone (NTBC) has offered a life-saving treatment that inhibits the upstream enzyme 4-hydroxyphenylpyruvate dioxygenase (HPD), thereby preventing production of downstream toxic metabolites. However, HT1 patients under NTBC therapy remain unable to degrade tyrosine. To control the disease and side-effects of the drug, HT1 patients need to take NTBC as an adjunct to a lifelong tyrosine and phenylalanine restricted diet. As a consequence of this strict therapeutic regime, drug compliance issues can arise with significant influence on patient health. In this study, we investigated the molecular impact of short-term NTBC therapy discontinuation on liver tissue of Fah-deficient mice. We found that after seven days of NTBC withdrawal, molecular pathways related to oxidative stress, glutathione metabolism, and liver regeneration were mostly affected. More specifically, NRF2-mediated oxidative stress response and several toxicological gene classes related to reactive oxygen species metabolism were significantly modulated. We observed that the expression of several key glutathione metabolism related genes including Slc7a11 and Ggt1 was highly increased after short-term NTBC therapy deprivation. This stress response was associated with the transcriptional activation of several markers of liver progenitor cells including Atf3, Cyr61, Ddr1, Epcam, Elovl7, and Glis3, indicating a concreted activation of liver regeneration early after NTBC withdrawal.

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Proteomic analysis of the oxidative stress response inCandida albicans

PROTEOMICS ◽

10.1002/pmic.200600575 ◽

2007 ◽

Vol 7 (5) ◽

pp. 686-697 ◽

Cited By ~ 61

Author(s):

Harald Kusch ◽

Susanne Engelmann ◽

Dirk Albrecht ◽

Joachim Morschhäuser ◽

Michael Hecker

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Proteomic Analysis ◽

Oxidative Stress Response ◽

Incandida Albicans

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Regulation of intracellular glutathione levels in erythrocytes infected with chloroquine-sensitive and chloroquine-resistant Plasmodium falciparum

Biochemical Journal ◽

10.1042/bj20020962 ◽

2002 ◽

Vol 368 (3) ◽

pp. 761-768 ◽

Cited By ~ 73

Author(s):

Svenja MEIERJOHANN ◽

Rolf D. WALTER ◽

Sylke MÜLLER

Keyword(s):

Oxidative Stress ◽

Red Blood Cells ◽

Blood Cells ◽

Differential Susceptibility ◽

Protozoan Parasite ◽

Chloroquine Resistance ◽

Glutathione Metabolism ◽

Glutathione Synthesis ◽

Intracellular Glutathione ◽

Glutamylcysteine Synthetase

Malaria is one of the most devastating tropical diseases despite the availability of numerous drugs acting against the protozoan parasite Plasmodium in its human host. However, the development of drug resistance renders most of the existing drugs useless. In the malaria parasite the tripeptide glutathione is not only involved in maintaining an adequate intracellular redox environment and protecting the cell against oxidative stress, but it has also been shown that it degrades non-polymerized ferriprotoporphyrin IX (FP IX) and is thus implicated in the development of chloroquine resistance. Glutathione levels in Plasmodium-infected red blood cells are regulated by glutathione synthesis, glutathione reduction and glutathione efflux. Therefore the effects of drugs that interfere with these metabolic processes were studied to establish possible differences in the regulation of the glutathione metabolism of a chloroquine-sensitive and a chloroquine-resistant strain of Plasmodiumfalciparum. Growth inhibition of P. falciparum 3D7 by d,l-buthionine-(S,R)sulphoximine (BSO), an inhibitor of γ-glutamylcysteine synthetase (γ-GCS), and by Methylene Blue (MB), an inhibitor of gluta thione reductase (GR), was significantly more pronounced than inhibition of P.falciparum Dd2 growth by these drugs. These results correlate with the higher levels of total glutathione in P. falciparum Dd2. Short-term incubations of Percoll-enriched trophozoite-infected red blood cells in the presence of BSO, MB and N,N1-bis(2-chloroethyl)-N-nitrosourea and subsequent determinations of γ-GCS activities, GR activities and glutathione disulphide efflux revealed that maintenance of intracellular glutathione in P. falciparum Dd2 is mainly dependent on glutathione synthesis whereas in P. falciparum 3D7 it is regulated via GR. Generally, P. falciparum Dd2 appears to be able to sustain its intracellular glutathione more efficiently than P. falciparum 3D7. In agreement with these findings is the differential susceptibility to oxidative stress of both parasite strains elicited by the glucose/glucose oxidase system.

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Proteomic analysis of seminal plasma from asthenozoospermia patients reveals proteins that affect oxidative stress responses and semen quality

Asian Journal of Andrology ◽

10.1038/aja.2009.26 ◽

2009 ◽

Vol 11 (4) ◽

pp. 484-491 ◽

Cited By ~ 61

Author(s):

Jun Wang ◽

Jian Wang ◽

Hua-Rong Zhang ◽

Hui-Juan Shi ◽

Duan Ma ◽

...

Keyword(s):

Oxidative Stress ◽

Seminal Plasma ◽

Stress Responses ◽

Proteomic Analysis ◽

Semen Quality ◽

Oxidative Stress Responses

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Proteomic Analysis of Mature Soybean Seeds from the Chernobyl Area Suggests Plant Adaptation to the Contaminated Environment

Journal of Proteome Research ◽

10.1021/pr900034u ◽

2009 ◽

Vol 8 (6) ◽

pp. 2915-2922 ◽

Cited By ~ 37

Author(s):

Maksym Danchenko ◽

Ludovit Skultety ◽

Namik M. Rashydov ◽

Valentyna V. Berezhna ◽

L’ubomír Mátel ◽

...

Keyword(s):

Proteomic Analysis ◽

Soybean Seeds ◽

Plant Adaptation

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Characterization of Storage Proteins in Wild (Glycine soja) and Cultivated (Glycine max) Soybean Seeds Using Proteomic Analysis

Journal of Agricultural and Food Chemistry ◽

10.1021/jf052954k ◽

2006 ◽

Vol 54 (8) ◽

pp. 3114-3120 ◽

Cited By ~ 73

Author(s):

Savithiry S. Natarajan ◽

Chenping Xu ◽

Hanhong Bae ◽

Thomas J. Caperna ◽

Wesley M. Garrett

Keyword(s):

Glycine Max ◽

Proteomic Analysis ◽

Storage Proteins ◽

Glycine Soja ◽

Soybean Seeds

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Proteomic Analyses of Soybean Root Tips During Germination

Protein and Peptide Letters ◽

10.2174/0929866521666140526152426 ◽

2014 ◽

Vol 21 (12) ◽

pp. 1308-1319

Author(s):

Setsuko Komatsu ◽

Myeong W. Oh ◽

Hee Y. Jang ◽

Soo J. Kwon ◽

Hye R. Kim ◽

...

Keyword(s):

Protein Synthesis ◽

Proteomic Analysis ◽

Plant Root ◽

Root Tip ◽

Root Tips ◽

Form Complex ◽

Molecular Weights ◽

Soybean Seedlings ◽

2D Gel ◽

Proteomic Techniques

Plant root systems form complex networks with the surrounding soil environment and are controlled by both internal and external factors. To better understand the function of root tips of soybean during germination, three proteomic techniques were used to analyze the protein profiles of root tip cells. Proteins were extracted from the root tips of 4-dayold soybean seedlings and analyzed using two-dimensional (2D) gel electrophoresis-based proteomics, SDS-gel based proteomics, and gel-free proteomics techniques. A total of 121, 862, and 341 proteins were identified in root tips using the 2D gel-based, SDS gel-based, and gel-free proteomic techniques, respectively. The proteins identified by 2D gel-based proteomic analysis were predominantly localized in the cytoplasm, whereas nuclear-localized proteins were most commonly identified by the SDS gel-based and gel-free proteomics techniques. Of the 862 proteins identified in the SDS gelbased proteomic analysis, 190 were protein synthesis-related proteins. Furthermore, 24 proteins identified using the 2Dgel based proteomic technique shifted between acidic and basic isoelectric points, and 2 proteins, heat shock protein 70.2 and AAA-type ATPase, displayed two different molecular weights at the same isoelectric point. Taken together, these results suggest that a number of proteins related to protein synthesis and modification are activated in the root tips of soybean seedlings during germination.

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