scholarly journals Vepris macrophylla (Baker) I. Verd Essential Oil: An Antifungal Agent against Phytopathogenic Fungi

2020 ◽  
Vol 21 (8) ◽  
pp. 2776 ◽  
Author(s):  
Laura Giamperi ◽  
Anahi Elena Ada Bucchini ◽  
Donata Ricci ◽  
Bruno Tirillini ◽  
Marcello Nicoletti ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Antifungal Agent ◽  
Alternaria Solani ◽  
Phytopathogenic Fungi ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Fungistatic Activity ◽  
Leaf Essential Oil ◽  
Main Components

Rutaceae are widely used in ethnomedicine to treat infectious diseases in humans and plants. In this study, the antifungal activity of the Vepris macrophylla leaf essential oil (VEO) and its main components, citral and citronellol, was evaluated against six phytopathogenic fungi. In addition, the possible action of VEO on the synthesis of mycotoxins was evaluated as well. To determine the antifungal activity of VEO we used the agar dilution method and VEO showed inhibitory activity against all the tested fungi. In particular, VEO resulted to be fungicidal against Phytophthora cryptogea and Fusarium avenaceum. For all other fungi VEO exhibited fungistatic activity and the weakest effect was observed on Alternaria solani. Citral was very effective against P. cryptogea, F. avenaceum, F. poae and F. graminearum. On the other hand, citronellol showed good activity towards P. cryptogea and F. avenaceum and weaker activity towards F. poae and F. graminearum. It can be concluded that VEO can be considered a promising antifungal agent, especially against P. cryptogea and F. avenaceum, suggesting a possible use in the formulation of new selective and natural fungicides.

scholarly journals In Vitro Antifungal Activity of Polysulfides-Rich Essential Oil of Ferula Latisecta Fruits against Human Pathogenic Dermatophytes

2008 ◽  
Vol 3 (9) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Mehrdad Iranshahi ◽  
Abdolmajid Fata ◽  
Bahareh Emami ◽  
Bibi Mohadeseh Jalalzadeh Shahri ◽  
Bibi Sedigheh Fazly Bazzaz
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution ◽  
Sulfur Containing ◽  
Type Strains ◽  
In Vitro Antifungal Activity

The increase in dermatophytoses and the fact that some patients do not respond well to therapy make it necessary to find new antifungal agents. As part of our ongoing studies on medicinal plants from Iran, we studied antidermatophytic activities of Ferula latisecta essential oil, which had shown considerable antifungal activity in preliminary antimicrobial screening. Antifungal activity was evaluated by determination of MIC values using the agar dilution method on type strains of Candida albicans and dermatophytes. The composition of the oil was characterized by GC and GC/MS analyses. The essential oil was rich in polysulfides (75.2%) and exhibited good activity against Trichophyton rubrum and T. verrucosom for about three weeks, with a MIC value 96 μg/mL. The oil showed antifungal activity, especially against dermatophytes, and the activity is probably related to the sulfur-containing components of the oil. This study has identified that the polysulfides-rich essential oil of Ferula latisecta fruits has activity against a range of human pathogenic dermatophytes, justifying future clinical trials to validate its use as a therapeutic alternative for dermatophytosis.

scholarly journals Chemical Composition and Antifungal Activity of the Essential Oil of Cotinus coggygria from Marche Region (Italy)

2018 ◽  
Vol 13 (9) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Daniele Fraternale ◽  
Donata Ricci
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Pathogenic Fungi ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Plant Pathogenic Fungi ◽  
Cotinus Coggygria ◽  
In Vitro Antifungal Activity

The present study reports the results of gas chromatography-mass spectrometry (GC/MS) analyses of the essential oil from flowering aerial parts of Cotinus coggygria Scoop. (Anacardiaceae), as well as its in vitro antifungal activity against nine plant pathogenic fungi. Moreover, the essential oil was evaluated for its antifungal activity using the agar dilution method, and also MICs (minimum inhibitory concentrations) and MFCs (minimum fungicidal concentrations) were determined. The major compounds identified by GC-MS were limonene (49.2%), (Z)-β-ocimene (13.6%), α-pinene (8.8%) and (E)-β-ocimene (5.9%). The oil showed in vitro antifungal activity against some species of the Fusarium genus, Botrytis cinerea, and Alternaria solani. Our study indicates that the oil of C. coggygria could be used as a control agent for plant pathogenic fungi in natural formulations.

scholarly journals Activity of Vitis vinifera Tendrils Extract Against Phytopathogenic Fungi

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Daniele Fraternale ◽  
Donata Ricci ◽  
Giancarlo Verardo ◽  
Andrea Gorassini ◽  
Vilberto Stocchi ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Vitis Vinifera ◽  
Fusarium Species ◽  
Ethanolic Extract ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Dilution Method ◽  
Vitis Vinifera L ◽  
Agar Dilution Method

The in vitro antifungal activity was determined of an ethanolic extract of Vitis vinifera L. tendrils (TVV) against ten plant pathogenic fungi, using the agar dilution method; activity was shown against all tested fungi. Fusarium species were the most sensitive with MIC values ranging from 250 to 300 ppm, while the basidiomycete fungus Rhizoctonia solani was the most resistant, with a MIC value of 500 ppm. Electrospray ionization tandem mass spectrometry (ESI-MSn) was used to obtain qualitative information on the main components of TVV. The high amount of polyphenolic compounds contained in TVV is likely to contribute significantly to its antifungal activity.

scholarly journals Antifungal Activities of Cymbopogon citratus Essential Oil against Aspergillus Species Isolated from Fermented Fish Products of Southern Benin

2019 ◽  
Author(s):  
R.G. Dègnon ◽  
A.C. Allagbé ◽  
E.S. Adjou ◽  
E. Dahouenon-Ahoussi
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Aspergillus Species ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Cymbopogon Citratus ◽  
Fermented Fish ◽  
Significant Difference ◽  
Fish Samples

Background: In Benin Republic, the conservation of fermented fishes for a long time is difficult due to the contamination of fungi, which lead to its rapid degradation. This experiment was conducted to evaluate the effect of Cymbopogon citratus Essential Oil (EO) against Aspergillus species isolated from fermented fish samples. Methods: Gas chromatography-mass spectrometry was used to determinate the chemical composition of the C. citratus EO. The agar dilution method was used to evaluate the antifungal activity of the C. citratus EO against Aspergillus species. Data were analyzed using SPSS, Chicago, IL, USA, version 10.0. Results: The major components of C. citratus EO were geranial (41.3%), neral (33.0%), myrcene (10.4%), and geraniol (6.6%). The dominant Aspergillus fungi isolated from the fermented fish samples were A. ochraceus, A. oryzae, A. fumigatus, and A. parasiticus. C. citratus EO exhibited considerable antifungal activity against the growth of fungi isolated from fermented fish samples. There was no significant difference between minimal inhibitory concentrations and minimal fungicidal concentrations of A. ochraceus and A. parasiticus (p>0.05). Conclusion: The findings of this research clearly indicate that the C. citratus EO may be a practical application in controlling the growth of Aspergillus species in fermented fish.

scholarly journals Chemical Analysis and Growth Inhibitory Effect Of Rosemary Plant on Aspergillus niger

2017 ◽  
Vol 2 (2) ◽  
pp. 151-154
Author(s):  
Tara Faeq M. Salih ◽  
Lana MA. Mohammed ◽  
Karzan Omer Qader
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Main Compound ◽  
Plant Extraction

The use of natural fungicides instead of synthetic ones covers is a present trend in mycological disease control. The quality and food safeties could be preserved by the addition of natural antifungal agents as essential oils. The aim of the present study was to assess the antifungal activity of certain essential oil against the genus of Aspergillus The oil of the aerial parts of Rosemary plant that collected from Sulaimani city was analyzed by using gas chromatography mass spectrometry. The antifungal activity of Rosemary plant extract against Aspergillus niger was assessed by using agar dilution method. The results showed identification of 17 compounds, with 80.62% of Benzene, 1-methoxy-4- (1-propenyl), 6.85% 1,3,6-Octatriene, 3,7-dimethyl-, (E), 5.16% 1,3,6-Octatriene, 3,7- dimethyl and 2.83% D-Limonene constitute the highest percentage of essential oil. The result of investigation the antifungal effect of Rosemary plant extraction dilutions showed that the essential oil has significant inhibitory effect on growth of Aspergillus niger in 10 %, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, and 28% dilutions with the average inhibitory growth percentage 25.91%, 25.60%, 28.96%, 28.96%, 30.18%, 32.31%, 28.04%, 28.65%, 31.40% and 31.70% respectively. Large percentage antifungal activities of Rosemary oil are related with Benzene, 1-methoxy-4-(1-propenyl) as the main compound. In conclusion, the essential oils exhibited a potential inhibition activity against toxic fungi and many invitro and invivo studies are requested to find more about the antifungal activity of these extracted oils.

scholarly journals Antifungal activity of Nepeta rtanjensis essential oil

2008 ◽  
Vol 73 (10) ◽  
pp. 961-965 ◽  
Author(s):  
Milica Grbic-Ljaljevic ◽  
Milos Stupar ◽  
Jelena Vukojevic ◽  
Marina Sokovic ◽  
Danijela Misic ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Trichoderma Viride ◽  
Cultivated Plants ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Alternaria Species ◽  
Agar Dilution ◽  
Bipolaris Spicifera ◽  
Main Component

The chemical composition and antifungal activity of the essential oil of an endemic Serbian plant Nepeta rtanjensis Diklic & Milojevic was studied. The essential oil was isolated from cultivated plants. Inhibition of mycelia growth of five micromycetes, two Alternaria species originally isolated from N. rtanjensis, Cladosporium cladosporoides, Trichoderma viride and Bipolaris spicifera, were tested using the agar dilution method. The essential oil of N. rtanjensis, the main component of which was 4aa,7a,7ab-nepetalactone, showed strong antifungal activity against all the tested micromycetes. The minimum inhibitory concentration of N. rtanjensis essential oil ranged from 0.6 to 1.4 ?g mL-1. The fungi most sensitive to the tested oil were Alternaria species, while Trichoderma viride was the most resistant.

scholarly journals In Vitro Antifungal and Topical Anti-Inflammatory Properties of Essential Oil from Wild-Growing Thymus vulgaris (Lamiaceae) Used for Medicinal Purposes in Algeria: A New Source of Carvacrol

2020 ◽  
Vol 88 (3) ◽  
pp. 33 ◽  
Author(s):  
Mohamed Nadjib Boukhatem ◽  
Noureldien H. E. Darwish ◽  
Thangirala Sudha ◽  
Siham Bahlouli ◽  
Dahbia Kellou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Fungal Infections ◽  
Positive Control ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Anti Inflammatory

The aim of this study is to investigate the Thymus vulgaris essential oil (TVEO) as an antifungal agent in aromatherapy and/or as an active ingredient in the prevention or management of topical inflammatory diseases. The chemical composition of TVEO was determined with gas chromatography and revealed the presence of 25 compounds. Carvacrol was found to be the major component (56.8%). Antifungal action of TVEO was determined in vitro by using different methods. By the disc diffusion method, TVEO showed more potent antifungal activity against Candida strains than the positive control. The diameter of inhibition zone (DIZ) varied from 34 to 60 mm for Candida yeasts. Significantly higher antifungal activity was observed in the vapor phase at lower quantities. Candida albicans and C. parapsilosis were the most susceptible strains to the oil vapor with DIZ varying from 35 to 90 mm. The minimum inhibitory concentrations (MIC) of yeast were determined with an agar dilution method and revealed that MIC varied from 0.3 to 0.15 µL/mL for yeast species. The topical anti-inflammatory potential of TVEO was also explored in vivo with the croton oil-induced ear edema assay. TVEO exhibited a potent anti-inflammatory effect at all doses (100, 10 and 2 mg/kg), which were statistically similar (p > 0.05) to the positive control. This activity was also confirmed at the cellular level with histopathology analysis. Our results suggest the potential application of this carvacrol-rich TVEO in the prevention and management of fungal infections and topical inflammation and deserve further investigation for clinical applications. Furthermore, while the mode of action remains mainly undetermined and should be studied.

scholarly journals Ammoides pusilla Essential Oil: A Potent Inhibitor of the Growth of Fusarium avenaceum and Its Enniatin Production

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6906
Author(s):  
Yasmine Chakroun ◽  
Souheib Oueslati ◽  
Vessela Atanasova ◽  
Florence Richard-Forget ◽  
Manef Abderrabba ◽  
...  
Keyword(s):  
Essential Oil ◽  
Mycelial Growth ◽  
Fusarium Species ◽  
Fusarium Avenaceum ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agricultural Commodities ◽  
Fungistatic Activity ◽  
Antifungal Potential ◽  
Agar Dilution

Enniatins are mycotoxins produced by Fusarium species contaminating cereals and various agricultural commodities. The co-occurrence of these mycotoxins in large quantities with other mycotoxins such as trichothecenes and the possible synergies in toxicity could lead to serious food safety problems. Using the agar dilution method, Ammoides pusilla was selected among eight Tunisian plants for the antifungal potential of its essential oil (EO) on Fusarium avenaceum mycelial growth and its production of enniatins. Two EO batches were produced and analyzed by GC/MS-MS. Their activities were measured using both contact assays and fumigant tests (estimated IC50 were 0.1 µL·mL−1 and 7.6 µL·L−1, respectively). The A. pusilla EOs and their volatiles inhibited the germination of spores and the mycelial growth, showing a fungistatic but not fungicidal activity. The accumulation of enniatins was also significantly reduced (estimated IC50 were 0.05 µL·mL−1 for the contact assays and 4.2 µL·L−1 for the fumigation assays). The most active batch of EO was richer in thymol, the main volatile compound found. Thymol used as fumigant showed a potent fungistatic activity but not a significant antimycotoxigenic activity. Overall, our data demonstrated the bioactivity of A. pusilla EO and its high potential to control F. avenaceum and its enniatins production in agricultural commodities.

scholarly journals Antifungal and Antibacterial Metabolites from a French Poplar Type Propolis

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Séverine Boisard ◽  
Anne-Marie Le Ray ◽  
Anne Landreau ◽  
Marie Kempf ◽  
Viviane Cassisa ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Gram Positive ◽  
Weak Activity ◽  
Agar Dilution

During this study, thein vitroantifungal and antibacterial activities of different extracts (aqueous and organic) obtained from a French propolis batch were evaluated. Antifungal activity was evaluated by broth microdilution on three pathogenic strains:Candida albicans, C. glabrata, andAspergillus fumigatus. Antibacterial activity was assayed using agar dilution method on 36 Gram-negative and Gram-positive strains includingStaphylococcus aureus. Organic extracts showed a significant antifungal activity againstC. albicansandC. glabrata(MIC80between 16 and 31 µg/mL) but only a weak activity towardsA. fumigatus(MIC80= 250 µg/mL). DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially againstS. aureus(SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC10030–97 µg/mL). A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.

Antifungal activity of dermadex-agents for the treatment of microsporia and trichophytia

2001 ◽  
Vol 82 (3) ◽  
pp. 214-214
Author(s):  
L. R. Zakieva ◽  
R. S. Garaev ◽  
M. T. Tremasov
Keyword(s):  
Antifungal Activity ◽  
Antifungal Agent ◽  
Medicinal Form ◽  
Toxicological Evaluation ◽  
Fungistatic Activity

The aim of our study was the toxicological evaluation of the drug Dermadex, which is a new medicinal form of the antifungal agent 2-mercantobenzothiazole, as well as the study of its fungicidal and fungistatic activity with the development of tactics for the treatment of microsporia and trichophytia.

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