scholarly journals Advanced Bioluminescence System for In Vivo Imaging with Brighter and Red-Shifted Light Emission

2020 ◽  
Vol 21 (18) ◽  
pp. 6538 ◽  
Author(s):  
Mizuki Endo ◽  
Takeaki Ozawa
Keyword(s):  
Near Infrared ◽  
Light Emission ◽  
Animal Experiments ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Infrared Light ◽  
High Signal ◽  
In Vivo Bioluminescence Imaging ◽  
Chemical Modulation

In vivo bioluminescence imaging (BLI), which is based on luminescence emitted by the luciferase–luciferin reaction, has enabled continuous monitoring of various biochemical processes in living animals. Bright luminescence with a high signal-to-background ratio, ideally red or near-infrared light as the emission maximum, is necessary for in vivo animal experiments. Various attempts have been undertaken to achieve this goal, including genetic engineering of luciferase, chemical modulation of luciferin, and utilization of bioluminescence resonance energy transfer (BRET). In this review, we overview a recent advance in the development of a bioluminescence system for in vivo BLI. We also specifically examine the improvement in bioluminescence intensity by mutagenic or chemical modulation on several beetle and marine luciferase bioluminescence systems. We further describe that intramolecular BRET enhances luminescence emission, with recent attempts for the development of red-shifted bioluminescence system, showing great potency in in vivo BLI. Perspectives for future improvement of bioluminescence systems are discussed.

Fluorescence resonance energy transfer (FRET) based nanoparticles composed of AIE luminogens and NIR dyes with enhanced three-photon near-infrared emission for in vivo brain angiography

Nanoscale ◽  
2018 ◽  
Vol 10 (21) ◽  
pp. 10025-10032 ◽  
Author(s):  
Wen Liu ◽  
Yalun Wang ◽  
Xiao Han ◽  
Ping Lu ◽  
Liang Zhu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Infrared Emission ◽  
Biological Imaging ◽  
High Contrast ◽  
Nir Dyes ◽  
Nir Fluorescence ◽  
Near Infrared Emission

Near-infrared (NIR) fluorescence is very important for high-contrast biological imaging of high-scattering tissues such as brain tissue.

Nanoparticles based on quantum dots and a luminol derivative: implications for in vivo imaging of hydrogen peroxide by chemiluminescence resonance energy transfer

2016 ◽  
Vol 52 (22) ◽  
pp. 4132-4135 ◽  
Author(s):  
Eun Sook Lee ◽  
V. G. Deepagan ◽  
Dong Gil You ◽  
Jueun Jeon ◽  
Gi-Ra Yi ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Energy Transfer ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Hybrid Nanoparticles ◽  
Infrared Wavelength ◽  
Luminol Derivative

Hybrid nanoparticles allow for imaging hydrogen peroxide via chemiluminescence resonance energy transfer in the near-infrared wavelength range.

scholarly journals Near-infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Cassandra L Stowe ◽  
Thomas A Burley ◽  
Helen Allan ◽  
Maria Vinci ◽  
Gabriela Kramer-Marek ◽  
...  
Keyword(s):  
Near Infrared ◽  
Bioluminescence Imaging ◽  
Light Emission ◽  
Small Animal ◽  
Firefly Luciferase ◽  
High Energy ◽  
Spectral Unmixing ◽  
Infrared Light ◽  
T Cell Therapy

Bioluminescence imaging (BLI) is ubiquitous in scientific research for the sensitive tracking of biological processes in small animal models. However, due to the attenuation of visible light by tissue, and the limited set of near-infrared bioluminescent enzymes, BLI is largely restricted to monitoring single processes in vivo. Here we show, that by combining stabilised colour mutants of firefly luciferase (FLuc) with the luciferin (LH2) analogue infraluciferin (iLH2), near-infrared dual BLI can be achieved in vivo. The X-ray crystal structure of FLuc with a high-energy intermediate analogue, 5’-O-[N-(dehydroinfraluciferyl)sulfamoyl] adenosine (iDLSA) provides insight into the FLuc-iLH2 reaction leading to near-infrared light emission. The spectral characterisation and unmixing validation studies reported here established that iLH2 is superior to LH2 for the spectral unmixing of bioluminescent signals in vivo; which led to this novel near-infrared dual BLI system being applied to monitor both tumour burden and CAR T cell therapy within a systemically induced mouse tumour model.

scholarly journals Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Arthur Taylor ◽  
Jack Sharkey ◽  
Antonius Plagge ◽  
Bettina Wilm ◽  
Patricia Murray
Keyword(s):  
Bioluminescence Imaging ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Firefly Luciferase ◽  
Cell Populations ◽  
Wide Range ◽  
In Vivo Bioluminescence Imaging ◽  
Multiple Cell

The ability to track the biodistribution and fate of multiple cell populations administered to rodents has the potential to facilitate the understanding of biological processes in a range of fields including regenerative medicine, oncology, and host/pathogen interactions. Bioluminescence imaging is an important tool for achieving this goal, but current protocols rely on systems that have poor sensitivity or require spectral decomposition. Here, we show that a bioluminescence resonance energy transfer reporter (BRET) based on NanoLuc and LSSmOrange in combination with firefly luciferase enables the unambiguous discrimination of two cell populations in vivo with high sensitivity. We insert each of these reporter genes into cells using lentiviral vectors and demonstrate the ability to monitor the cells’ biodistribution under a wide range of administration conditions, including the venous or arterial route, and in different tissues including the brain, liver, kidneys, and tumours. Our protocol allows for the imaging of two cell populations in the same imaging session, facilitating the overlay of the signals and the identification of anatomical positions where they colocalise. Finally, we provide a method for postmortem confirmation of the presence of each cell population in excised organs.

scholarly journals Near-Infrared Resonance Energy Transfer Glucose Biosensors in Hybrid Microcapsule Carriers

2008 ◽  
Vol 2008 ◽  
pp. 1-11 ◽  
Author(s):  
Swetha Chinnayelka ◽  
Huiguang Zhu ◽  
Mike McShane
Keyword(s):  
Energy Transfer ◽  
Continuous Monitoring ◽  
Near Infrared ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Competitive Binding ◽  
Competitive Binding Assay ◽  
Sensing Systems ◽  
Recent Developments

Fluorescence-based sensing systems offer potential for noninvasive monitoring with implantable devices, but require carrier technologies that provide suitable immobilization, accessibility, and biocompatibility. Recent developments towards this goal include a competitive binding assay for glucose that has been encapsulated in semipermeable microcapsule carriers. This paper describes an extension of this work to increase the applicability to in vivo monitoring, wherein two significant developments are described: (1) a near-infrared resonance energy transfer system for transducing glucose concentration, and (2) novel hybrid organic-inorganic crosslinked microcapsules as carriers. The quenching-based assay is a competitive binding (CB) system based on apo-glucose oxidase (AG) as the receptor and dextran as the competitive ligand. The encapsulated quencher-labeled dextran and near infrared donor-labeled glucose receptor showed a stable and reversible response with tunable sensitivity of 1–5%/mM over the physiological range, making these transducers attractive for continuous monitoring for biomedical applications.

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