scholarly journals Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in Arabidopsis

2021 ◽  
Vol 22 (7) ◽  
pp. 3398
Author(s):  
Ming Qin ◽  
Xiaoyan Li ◽  
Shaohua Tang ◽  
Yinglin Huang ◽  
Ling Li ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Stress Responses ◽  
Expression Patterns ◽  
Plant Stress ◽  
Limited Information ◽  
Response Gene ◽  
Stress Memory ◽  
Gene Type ◽  
Aba Content

Arachis hypogaea abscisic acid transporter like-1 (AhATL1) modulates abscisic acid (ABA) sensitivity by specifically influencing the importing of ABA into cells, and is a key player in plant stress responses. However, there is limited information on ABA transporters in crops. In this study, we found that the level of AhATL1 expression and AhATL1 distribution increased more rapidly in the second drought (D2) compared with in the first drought (D1). Compared with the first recovery (R1), the AhATL1 expression level and ABA content remained at a higher level during the second recovery (R2). The heterologous overexpression of AhATL1 in Arabidopsis changed the expression pattern of certain memory genes and changed the post response gene type into the memory gene type. Regarding the proline and water content of Col (Arabidopsis thaliana L. Heynh., Col-0), atabcg22, and AhATL1-OX during drought training, the second drought (D2) was more severe than the first drought (D1), which was more conducive to maintaining the cell osmotic balance and resisting drought. In summary, drought stress memory resulted in a rapid increase in the AhATL1 expression and AhATL1 distribution level, and then raised the endogenous ABA content and changed the post response gene type into the memory gene type, which enhanced the drought resistance and recovery ability.

Genome-wide identification of citrus calmodulin-like genes and their expression in response to arbuscular mycorrhizal fungi colonization and drought

2021 ◽  
Author(s):  
Bo Shu ◽  
YaChao Xie ◽  
Fei Zhang ◽  
Dejian Zhang ◽  
Chunyan Liu ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Arbuscular Mycorrhizal Fungi ◽  
Stress Responses ◽  
Mycorrhizal Fungi ◽  
Expression Patterns ◽  
Arbuscular Mycorrhizal ◽  
Biotic And Abiotic Stress ◽  
Genome Wide ◽  
A Genome

Calmodulin-like (CML) proteins represent a diverse family of protein in plants, and play significant roles in biotic and abiotic stress responses. However, the involvement of citrus CMLs in plant responses to drought stress (abiotic stress) and arbuscular mycorrhizal fungi (AMF) colonization remain relatively unknown. We characterized the citrus CML genes by analyzing the EF-hand domains and a genome-wide search, and identified a total of 38 such genes, distributed across at least nine chromosomes. Six tandem duplication clusters were observed in the CsCMLs, and 12 CsCMLs exhibited syntenic relationships with Arabidopsis thaliana CMLs. Gene expression analysis showed that 29 CsCMLs were expressed in the roots, and exhibited differential expression patterns. The regulation of CsCMLs expression was not consistent with the cis-elements identified in their promoters. CsCML2, 3, and 5 were upregulated in response to drought stress, and AMF colonization repressed the expression of CsCML7, 9, 12, 13,20, 27, 28, and 35,and induced that of CsCML1, 2, 3, 5, 8, 10, 11, 14, 15, 16, 18, 25, 30, 33, and 37. Furthermore, AMF colonization and drought stress exerted a synergistic effect, evident from the enhanced repression of CsCML7, 9, 12, 13, 27, 28, and 35 and enhanced expression of CsCML2, 3, and 5 under AMF colonization and drought stress. The present study provides valuable insights into the CsCML gene family and its responses to AMF colonization and drought stress.

scholarly journals Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

2020 ◽  
Vol 21 (19) ◽  
pp. 7322
Author(s):  
Yunting Zhang ◽  
Mengwen Luo ◽  
Lijuan Cheng ◽  
Yuanxiu Lin ◽  
Qing Chen ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Responses ◽  
Expression Patterns ◽  
Plant Stress ◽  
Bioinformatic Analysis ◽  
Ros Generation ◽  
Ros Scavenging ◽  
Dehydrogenase Gene ◽  
Plant Stress Responses ◽  
Glucose 6 Phosphate Dehydrogenase

Glucose-6-phosphate dehydrogenase (G6PDH) plays an important role in plant stress responses. Here, five FaG6PDH sequences were obtained in strawberry, designated as FaG6PDH-CY, FaG6PDH-P1, FaG6PDH-P1.1, FaG6PDH-P2 and FaG6PDH-P0, which were divided into cytosolic (CY) and plastidic (P) isoforms based on the bioinformatic analysis. The respective FaG6PDH genes had distinct expression patterns in all tissues and at different stages of fruit development. Notably, FaG6PDH-CY was the most highly expressed gene among five FaG6PDH members, indicating it encoded the major G6PDH isoform throughout the plant. FaG6PDH positively regulated cold tolerance in strawberry. Inhibition of its activity gave rise to greater cold-induced injury in plant. The FaG6PDH-CY transcript had a significant increase under cold stress, similar to the G6PDH enzyme activity, suggesting a principal participant in response to cold stress. Further study showed that the low-temperature responsiveness (LTR) element in FaG6PDH-CY promoter can promote the gene expression when plant encountered cold stimuli. Besides, FaG6PDH-CY was involved in regulating cold-induced activation of antioxidant enzyme genes (FaSOD, FaCAT, FaAPX and FaGR) and RBOH-dependent ROS generation. The elevated FaG6PDH-CY enhanced ROS-scavenging capability of antioxidant enzymes to suppress ROS excessive accumulation and relieved the oxidative damage, eventually improving the strawberry resistance to cold stress.

scholarly journals An Arabidopsis Glutathione Peroxidase Functions as Both a Redox Transducer and a Scavenger in Abscisic Acid and Drought Stress Responses

2006 ◽  
Vol 18 (10) ◽  
pp. 2749-2766 ◽  
Author(s):  
Yuchen Miao ◽  
Dong Lv ◽  
Pengcheng Wang ◽  
Xue-Chen Wang ◽  
Jia Chen ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Glutathione Peroxidase ◽  
Stress Responses

scholarly journals Broad and Complex Roles of NBR1-Mediated Selective Autophagy in Plant Stress Responses

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2562
Author(s):  
Yan Zhang ◽  
Zhixiang Chen
Keyword(s):  
Stress Responses ◽  
Mammalian Cells ◽  
Plant Stress ◽  
Protein Aggregates ◽  
Degradation Pathway ◽  
Plant Responses ◽  
Stress Memory ◽  
Selective Autophagy ◽  
Cargo Proteins ◽  
Plant Stress Responses

Selective autophagy is a highly regulated degradation pathway for the removal of specific damaged or unwanted cellular components and organelles such as protein aggregates. Cargo selectivity in selective autophagy relies on the action of cargo receptors and adaptors. In mammalian cells, two structurally related proteins p62 and NBR1 act as cargo receptors for selective autophagy of ubiquitinated proteins including aggregation-prone proteins in aggrephagy. Plant NBR1 is the structural and functional homolog of mammalian p62 and NBR1. Since its first reports almost ten years ago, plant NBR1 has been well established to function as a cargo receptor for selective autophagy of stress-induced protein aggregates and play an important role in plant responses to a broad spectrum of stress conditions including heat, salt and drought. Over the past several years, important progress has been made in the discovery of specific cargo proteins of plant NBR1 and their roles in the regulation of plant heat stress memory, plant-viral interaction and special protein secretion. There is also new evidence for a possible role of NBR1 in stress-induced pexophagy, sulfur nutrient responses and abscisic acid signaling. In this review, we summarize these progresses and discuss the potential significance of NBR1-mediated selective autophagy in broad plant responses to both biotic and abiotic stresses.

The poplar R2R3 MYB transcription factor PtrMYB94 coordinates with abscisic acid signaling to improve drought tolerance in plants

2019 ◽  
Vol 40 (1) ◽  
pp. 46-59 ◽  
Author(s):  
Qing Fang ◽  
Xianqiang Wang ◽  
Haiyang Wang ◽  
Xiaowen Tang ◽  
Chi Liu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Populus Trichocarpa ◽  
Myb Transcription Factor ◽  
Exposure Condition ◽  
Rt Pcr ◽  
Stress Regulation ◽  
Large Gene ◽  
R2r3 Myb ◽  
Aba Content

Abstract In plants, R2R3 MYB transcription factors (TFs) consist of one large gene family and are involved in the regulation of many developmental processes and various stresses. However, the functions of most of MYB TFs in woody plants remain unknown. Here, PtrMYB94, an R2R3 MYB TF from Populus trichocarpa, is characterized to be involved in the regulation of drought responses and abscisic acid (ABA) signaling. PtrMYB94 encodes a nuclear-localized R2R3 MYB TF. RT-PCR results showed that the PtrMYB94 transcripts were relatively abundant in leaves and stems, and were induced rapidly in response to dehydration stress. Overexpression of PtrMYB94 improved plant drought responses, suggesting that this MYB TF may functionally regulate poplar adaptability to drought stress. Furthermore, the analysis of transcriptional expression and PtrMYB94 promoter: GUS activity showed that PtrMYB94 responded to ABA induction. PtrMYB94-overexpressing plants exhibited the inhibition of seed germination compared with the wild-type (WT) control under ABA exposure condition. The ABA content was evidently increased in the PtrMYB94-overexpressing plants relative to the WT plants. In addition, transcript levels of several ABA- and drought-responsive genes, such as ABA1 and DREB2B, were up-regulated. Taken together, our results suggest that PtrMYB94 is involved in an ABA-dependent drought stress regulation in Populus.

scholarly journals Benzyladenine and Gibberellic Acid Application Prevents Abscisic Acid-induced Leaf Chlorosis in Pansy and Viola

HortScience ◽  
2010 ◽  
Vol 45 (6) ◽  
pp. 925-933 ◽  
Author(s):  
Nicole L. Waterland ◽  
John J. Finer ◽  
Michelle L. Jones
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Gibberellic Acid ◽  
Stress Responses ◽  
Water Loss ◽  
Leaf Chlorosis ◽  
Bedding Plant ◽  
Leaf Yellowing ◽  
Negative Side Effects ◽  
The Individual

Drought stress during the shipping and retailing of floriculture crops can reduce postproduction shelf life and marketability. The plant hormone abscisic acid (ABA) mediates drought stress responses by closing stomata and reducing water loss. Applications of exogenous s-ABA effectively reduce water loss and allow a variety of species to survive temporary periods of drought stress. Unfortunately, s-ABA application can also lead to leaf chlorosis, which reduces the overall quality of economically important bedding plant species, including Viola ×wittrockiana (pansy). The goal of this research was to determine how to prevent s-ABA-induced leaf chlorosis in pansy and a closely related species, Viola cornuta (viola). All concentrations of both spray (250 or 500 mg·L−1) and drench (125 or 250 mg·L−1) s-ABA applications induced leaf yellowing. Young plants at the plug stage and 11-cm finished plants with one to two open flowers were further evaluated to determine if the developmental stage of the plants influenced s-ABA effectiveness or the development of negative side effects. Both plugs and finished pansies and violas developed leaf chlorosis after s-ABA applications, but symptoms were generally more severe in finished plants. The individual application of benzyladenine (BA), gibberellic acid (GA4+7), or the ethylene perception inhibitor, 1-methylcyclopropene, before s-ABA application had no effect on the development of s-ABA-induced leaf chlorosis. However, applications of 5 or 10 mg·L−1 BA and GA4+7 as a mixture (BA + GA4+7) before a drench or spray application of s-ABA prevented leaf chlorosis. The application of s-ABA and BA + GA4+7 would allow floriculture crops to tolerate temporary periods of drought stress without any loss of postproduction quality.

scholarly journals Drought Survival and Recuperative Ability of Bentgrass Species Associated with Changes in Abscisic Acid and Cytokinin Production

2007 ◽  
Vol 132 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Michelle DaCosta ◽  
Bingru Huang
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Creeping Bentgrass ◽  
Primary Objective ◽  
Rapid Decline ◽  
Plant Responses ◽  
Severe Drought ◽  
Cytokinin Content ◽  
Drought Survival ◽  
Aba Content

Abscisic acid (ABA) and cytokinins are two groups of plant hormones that play important roles in regulating plant responses to decreases in soil water availability. The primary objective for this study was to determine whether species variability in drought survival and recovery for colonial bentgrass (Agrostis capillaris L.), creeping bentgrass (A. stolonifera L.), and velvet bentgrass (A. canina L.) were related to changes in ABA and cytokinin content. Plants of ‘Tiger II’ colonial bentgrass, ‘L-93’ creeping bentgrass, and ‘Greenwich’ velvet bentgrass were subjected to two soil moisture treatments: 1) well-watered controls, irrigated three times per week; and 2) drought, irrigation completely withheld for 16 days. For recovery, previously drought-stressed plants were rewatered and irrigated three times per week to evaluate the recovery potential for each species. Drought stress resulted in significant declines in turf quality (TQ), shoot extension rates, canopy net photosynthetic rate (Pn), daily evapotranspiration rate (ET), and cytokinin content, and significant increases in ABA content for all three bentgrass species. Velvet bentgrass exhibited less severe drought injury, as exhibited by higher TQ, Pn, and daily ET rate compared with colonial bentgrass and creeping bentgrass. Velvet bentgrass also had significantly less ABA accumulation, which could allow for continued gas exchange and sustained plant survival during drought stress compared with colonial bentgrass and creeping bentgrass. Upon rewatering after drought stress, colonial bentgrass exhibited more rapid recovery in turfgrass growth and water use compared with creeping bentgrass and velvet bentgrass. The higher recuperative ability of colonial bentgrass could be associated with its more rapid decline in ABA content and increases in cytokinin content compared with creeping bentgrass and velvet bentgrass.

scholarly journals Comparative hormonal regulatory pathway of the drought responses in relation to glutamate-mediated proline metabolism in Brassica napus

2019 ◽  
Author(s):  
Van Hien La ◽  
Bok-Rye Lee ◽  
Md. Tabibul Islam ◽  
Sang-Hyun Park ◽  
Dong-Won Bae ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Responses ◽  
Plant Stress ◽  
Proline Accumulation ◽  
List Type ◽  
Dependent Manner ◽  
Proline Metabolism ◽  
Regulatory Pathway ◽  
Plant Stress Responses ◽  
Enhanced Expression

AbstractProline metabolism influences metabolic and signaling pathway in regulating plant stress responses. This study aimed to characterize the physiological significance of glutamate (Glu)-mediated proline metabolism in the drought stress responses, focusing on the hormonal regulatory pathway. The responses of cytosolic Ca2+ signaling, proline metabolism and redox components to the exogenous application of Glu in well-watered or drought-stressed plants were interpreted in relation to endogenous hormone status and their signaling genes. Drought-enhanced abscisic acid (ABA) were concomitant with ROS and proline accumulation, accompanied by decreased NAD(P)H/NAD(P)+ and GSH/GSSG ratios. Exogenous Glu-feeding under drought resulted in an increase of salicylic acid (SA) with an antagonistic decrease of ABA. Glu-enhanced SA coincided with the highest expression of SA synthesis related gene ICS1 and Ca2+-dependent protein kinase CPK5. SA-enhanced CPK5 expression was closely associated with further enhancement of proline synthesis-related genes (P5CS1, P5CS2, and P5CR) expression. The Glu-activated proline synthesis was responsible for the reset of reducing potential with enhanced expression of redox regulating genes TRXh5 and GRXC9 in a SA-mediated NPR1- and/or PR1-dependent manner. These results clearly indicate that Glu-activated interplay between SA- and CPK5-signaling and Glu-enhanced proline synthesis are crucial in the amelioration of drought stress in B. napus.HighlightDrought-induced oxidative stress and symptom are developed by ABA-dependent mannerGlu-application increases endogenous SA level with an antagonistic decrease of ABADrought-induced proline accumulation was further enhanced by exogenous Glu-applicationGlu-enhanced proline synthesis accompanied with SA-mediated regulatory pathwayGlu-enhanced SA-modulated proline metabolism is an integrated process of redox control

scholarly journals Characterization and expression analysis of growth regulating factor (GRF) family genes in cucumber

2018 ◽  
Vol 70 (4) ◽  
pp. 629-637 ◽  
Author(s):  
Yong Zhou ◽  
Lingli Ge ◽  
Guanghua Li ◽  
Lunwei Jiang ◽  
Yingui Yang
Keyword(s):  
Stress Responses ◽  
Expression Patterns ◽  
Plant Stress ◽  
Biological Processes ◽  
Transcriptome Data ◽  
Conserved Domains ◽  
Plant Stress Responses ◽  
Salt And Drought Stress ◽  
Profiling Analysis ◽  
Different Tissues

The growth regulating factor (GRF) family is a conserved class of transcription factors involved in various biological processes in plants. However, there have been only a few studies of the GRF family genes in cucumber, Cucumis sativus (Cs). In this study, we identified and characterized 8 CsGRF genes in cucumber. Two highly conserved domains, QLQ and WRC, were identified to be present in all CsGRF proteins. In addition, three less conserved domains (FFD, TQL, and GGPL) were also detected in some CsGRF members. Based on phylogenetic analysis, the GRF genes from cucumber, Arabidopsis, tomato, rice and maize could be classified into 10 groups, and CsGRFs were clustered closer with the GRF genes from dicots (Arabidopsis and tomato) than with those from monocots (rice and maize). Promoter analysis revealed that the CsGRF genes were involved in cucumber growth and development as well as in responses to various hormones and stresses. Transcriptome data showed that the CsGRF genes have distinct expression patterns in different tissues, especially in ovaries and leaves. Expression profiling analysis indicated that all CsGRF genes were responsive to salt and drought stress treatments. These results demonstrate that the cucumber GRF gene family may function in organ development and plant stress responses.

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