The poplar R2R3 MYB transcription factor PtrMYB94 coordinates with abscisic acid signaling to improve drought tolerance in plants

2019 ◽  
Vol 40 (1) ◽  
pp. 46-59 ◽  
Author(s):  
Qing Fang ◽  
Xianqiang Wang ◽  
Haiyang Wang ◽  
Xiaowen Tang ◽  
Chi Liu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Populus Trichocarpa ◽  
Myb Transcription Factor ◽  
Exposure Condition ◽  
Rt Pcr ◽  
Stress Regulation ◽  
Large Gene ◽  
R2r3 Myb ◽  
Aba Content

Abstract In plants, R2R3 MYB transcription factors (TFs) consist of one large gene family and are involved in the regulation of many developmental processes and various stresses. However, the functions of most of MYB TFs in woody plants remain unknown. Here, PtrMYB94, an R2R3 MYB TF from Populus trichocarpa, is characterized to be involved in the regulation of drought responses and abscisic acid (ABA) signaling. PtrMYB94 encodes a nuclear-localized R2R3 MYB TF. RT-PCR results showed that the PtrMYB94 transcripts were relatively abundant in leaves and stems, and were induced rapidly in response to dehydration stress. Overexpression of PtrMYB94 improved plant drought responses, suggesting that this MYB TF may functionally regulate poplar adaptability to drought stress. Furthermore, the analysis of transcriptional expression and PtrMYB94 promoter: GUS activity showed that PtrMYB94 responded to ABA induction. PtrMYB94-overexpressing plants exhibited the inhibition of seed germination compared with the wild-type (WT) control under ABA exposure condition. The ABA content was evidently increased in the PtrMYB94-overexpressing plants relative to the WT plants. In addition, transcript levels of several ABA- and drought-responsive genes, such as ABA1 and DREB2B, were up-regulated. Taken together, our results suggest that PtrMYB94 is involved in an ABA-dependent drought stress regulation in Populus.

scholarly journals A R2R3-MYB Transcription Factor Gene, BpMYB123, Regulates BpLEA14 to Improve Drought Tolerance in Betula platyphylla

2021 ◽  
Vol 12 ◽  
Author(s):  
Kaiwen Lv ◽  
Hairong Wei ◽  
Guifeng Liu
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Crop Production ◽  
Myb Transcription Factor ◽  
Betula Platyphylla ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Negative Impacts ◽  
R2r3 Myb

Drought stress causes various negative impacts on plant growth and crop production. R2R3-MYB transcription factors (TFs) play crucial roles in the response to abiotic stress. However, their functions in Betula platyphylla haven’t been fully investigated. In this study, a R2R3 MYB transcription factor gene, BpMYB123, was identified from Betula platyphylla and reveals its significant role in drought stress. Overexpression of BpMYB123 enhances tolerance to drought stress in contrast to repression of BpMYB123 by RNA interference (RNAi) in transgenic experiment. The overexpression lines increased peroxidase (POD) and superoxide dismatase (SOD) activities, while decreased hydrogen peroxide (H2O2), superoxide radicals (O2–), electrolyte leakage (EL) and malondialdehyde (MDA) contents. Our study showed that overexpression of BpMYB123 increased BpLEA14 gene expression up to 20-fold due to BpMYB123 directly binding to the MYB1AT element of BpLEA14 promoter. These results indicate that BpMYB123 acts as a regulator via regulating BpLEA14 to improve drought tolerance in birch.

scholarly journals Drought Survival and Recuperative Ability of Bentgrass Species Associated with Changes in Abscisic Acid and Cytokinin Production

2007 ◽  
Vol 132 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Michelle DaCosta ◽  
Bingru Huang
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Creeping Bentgrass ◽  
Primary Objective ◽  
Rapid Decline ◽  
Plant Responses ◽  
Severe Drought ◽  
Cytokinin Content ◽  
Drought Survival ◽  
Aba Content

Abscisic acid (ABA) and cytokinins are two groups of plant hormones that play important roles in regulating plant responses to decreases in soil water availability. The primary objective for this study was to determine whether species variability in drought survival and recovery for colonial bentgrass (Agrostis capillaris L.), creeping bentgrass (A. stolonifera L.), and velvet bentgrass (A. canina L.) were related to changes in ABA and cytokinin content. Plants of ‘Tiger II’ colonial bentgrass, ‘L-93’ creeping bentgrass, and ‘Greenwich’ velvet bentgrass were subjected to two soil moisture treatments: 1) well-watered controls, irrigated three times per week; and 2) drought, irrigation completely withheld for 16 days. For recovery, previously drought-stressed plants were rewatered and irrigated three times per week to evaluate the recovery potential for each species. Drought stress resulted in significant declines in turf quality (TQ), shoot extension rates, canopy net photosynthetic rate (Pn), daily evapotranspiration rate (ET), and cytokinin content, and significant increases in ABA content for all three bentgrass species. Velvet bentgrass exhibited less severe drought injury, as exhibited by higher TQ, Pn, and daily ET rate compared with colonial bentgrass and creeping bentgrass. Velvet bentgrass also had significantly less ABA accumulation, which could allow for continued gas exchange and sustained plant survival during drought stress compared with colonial bentgrass and creeping bentgrass. Upon rewatering after drought stress, colonial bentgrass exhibited more rapid recovery in turfgrass growth and water use compared with creeping bentgrass and velvet bentgrass. The higher recuperative ability of colonial bentgrass could be associated with its more rapid decline in ABA content and increases in cytokinin content compared with creeping bentgrass and velvet bentgrass.

scholarly journals   Role of abscisic acid and drought stress on the activities of antioxidant enzymes in wheat

2012 ◽  
Vol 58 (No. 4) ◽  
pp. 181-185 ◽  
Author(s):  
A. Bano ◽  
F. Ullah ◽  
A. Nosheen
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Seed Treatment ◽  
Oxygen Species ◽  
Wheat Cultivars ◽  
Drought Tolerant ◽  
Relative Water ◽  
Aba Content ◽  
Endogenous Aba

The effect of drought stress and abscisic acid (ABA) applied at tillering stage (55 days after sowing) was compared in 2 wheat cultivars differing in drought tolerance. The activities of superoxide dismutase (SOD) and peroxidase (POD) and contents of endogenous ABA in plants were measured at 3 days of drought stress in cv. Chakwal-97 (drought tolerant) and cv. Punjab-96 (drought susceptible). ABA was applied at 10<sup>&ndash;6</sup> mol/L as presowing seed treatment for 18 h. Drought tolerant cultivar has a more efficient mechanism to scavenge reactive oxygen species as shown by a significant increase in the activity of antioxidant enzyme SOD. Under drought stress, ABA significantly increased the activities of SOD and POD, showing a significant decline on rewatering. The relative water content was significantly increased by ABA priming under drought stress in both wheat cultivars. The sensitive cultivar exhibiting lower endogenous ABA content was more responsive to ABA priming. On rewatering, the magnitude of recovery from drought stress was greater in tolerant cultivar. ABA was highly effective in improving grain weight of tolerant cultivar under drought stress. &nbsp;

scholarly journals Genotypic Variation in Abscisic Acid Accumulation, Water Relations, and Gas Exchange for Kentucky Bluegrass Exposed to Drought Stress

2003 ◽  
Vol 128 (3) ◽  
pp. 349-355 ◽  
Author(s):  
Zhaolong Wang ◽  
Bingru Huang
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Gas Exchange ◽  
Water Relations ◽  
Drought Resistance ◽  
Accumulation Rate ◽  
Kentucky Bluegrass ◽  
Stress Conditions ◽  
Turf Quality ◽  
Aba Content

Drought is a major limiting factor for turfgrass growth. Understanding genetic variations in physiological responses of turfgrass to drought stress would facilitate breeding and management programs to improve drought resistance. This study was designed to evaluate responses of abscisic acid (ABA) accumulation, water relations, and gas exchange to drought stress in four Kentucky bluegrass (Poa pratensis L.) cultivars differing in drought resistance. Plants of `Midnight' and `A82-204' (drought resistant) and `Brilliant' and `RSP' (drought susceptible) were grown under well-watered (control) or drought stress conditions for 25 days in growth chambers. Turf quality, leaf water potential (Ψleaf), relative water content (RWC), leaf net photosynthesis rate (Pn), and stomatal conductance (gs) declined, while electrolyte leakage (EL) increased during drought progression in all cultivars. The magnitudes of the change in these parameters were greater for `RSP' and `Brilliant' than for `Midnight' and `A82-204'. Leaf ABA content in `RSP' and `Brilliant' increased sharply after 2 days of stress to as much as 34 times the control level at 10 days of drought. Leaf ABA content in `Midnight' and `A82-204' also increased with drought, but to a lesser extent than in the other two cultivars. Leaf ABA level was negatively correlated with Ψleaf and gs. `A82-204' had a significantly lower ABA accumulation rate with changes in Ψleaf during drought compared to `Midnight', `RSP' and `Brilliant'; however, no differences in ABA accumulation rate were detected among the latter three cultivars. In addition, leaf gs was more sensitive to changes in ABA accumulation in `Midnight' and `A82-204' than in `RSP' and `Brilliant'. These results demonstrated that drought tolerant cultivars of Kentucky bluegrass were characterized by lower ABA accumulation and less severe decline in Ψleaf, Pn, gs, and turf quality during drought stress than drought sensitive cultivars. Drought tolerance of Kentucky bluegrass could be related to sensitivity of stomata to endogenous accumulation of ABA under drought stress conditions.

scholarly journals A sugarcane R2R3-MYB transcription factor gene is alternatively spliced during drought stress

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jinlong Guo ◽  
Hui Ling ◽  
Jingjing Ma ◽  
Yun Chen ◽  
Yachun Su ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Myb Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Alternatively Spliced ◽  
R2r3 Myb

scholarly journals Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in Arabidopsis

2021 ◽  
Vol 22 (7) ◽  
pp. 3398
Author(s):  
Ming Qin ◽  
Xiaoyan Li ◽  
Shaohua Tang ◽  
Yinglin Huang ◽  
Ling Li ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Stress Responses ◽  
Expression Patterns ◽  
Plant Stress ◽  
Limited Information ◽  
Response Gene ◽  
Stress Memory ◽  
Gene Type ◽  
Aba Content

Arachis hypogaea abscisic acid transporter like-1 (AhATL1) modulates abscisic acid (ABA) sensitivity by specifically influencing the importing of ABA into cells, and is a key player in plant stress responses. However, there is limited information on ABA transporters in crops. In this study, we found that the level of AhATL1 expression and AhATL1 distribution increased more rapidly in the second drought (D2) compared with in the first drought (D1). Compared with the first recovery (R1), the AhATL1 expression level and ABA content remained at a higher level during the second recovery (R2). The heterologous overexpression of AhATL1 in Arabidopsis changed the expression pattern of certain memory genes and changed the post response gene type into the memory gene type. Regarding the proline and water content of Col (Arabidopsis thaliana L. Heynh., Col-0), atabcg22, and AhATL1-OX during drought training, the second drought (D2) was more severe than the first drought (D1), which was more conducive to maintaining the cell osmotic balance and resisting drought. In summary, drought stress memory resulted in a rapid increase in the AhATL1 expression and AhATL1 distribution level, and then raised the endogenous ABA content and changed the post response gene type into the memory gene type, which enhanced the drought resistance and recovery ability.

scholarly journals Maize ZmbZIP33 Is Involved in Drought Resistance and Recovery Ability Through an Abscisic Acid-Dependent Signaling Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Liru Cao ◽  
Xiaomin Lu ◽  
Guorui Wang ◽  
Qianjin Zhang ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Chlorophyll Content ◽  
Signaling Pathway ◽  
Drought Resistance ◽  
Aba Signaling ◽  
Maize Leaves ◽  
Aba Content ◽  
Aba Biosynthesis ◽  
Dependent Signaling Pathway

Analyzing the transcriptome of maize leaves under drought stress and rewatering conditions revealed that transcription factors were involved in this process, among which ZmbZIP33 of the ABSCISIC ACID-INSENSITIVE 5-like protein 5 family was induced to significantly up-regulated. The functional mechanism of ZmbZIP33 in Abscisic acd (ABA) signaling pathway and its response to drought stress and rewatering has not been studied yet. The present study found that ZmbZIP33 contains a DNA-binding and dimerization domain, has transcriptional activation activity, and is highly homologous to SbABI1,SitbZIP68 and OsABA1. The expression of ZmbZIP33 is strongly up-regulated by drought, high salt, high temperature, and ABA treatments. Overexpression of ZmbZIP33 remarkably increased chlorophyll content and root length after drought stress and rewatering, and, moreover, cause an accumulation of ABA content, thereby improving drought resistance and recovery ability in Arabidopsis. However, silencing the expression of ZmbZIP33 (BMV-ZmbZIP33) remarkably decreased chlorophyll content, ABA content, superoxide dismutase and peroxidase activities, and increased stomatal opening and water loss rate compared with BMV (control). It showed that silencing ZmbZIP33 lead to reduced drought resistance and recovery ability of maize. ABA sensitivity analysis found that 0.5 and 1 μmol/L treatments severely inhibited the root development of overexpression ZmbZIP33 transgenic Arabidopsis. However, the root growth of BMV was greatly inhibited for 1 and 5μmol/L ABA treatments, but not for BMV-ZmbZIP33. Subcellular localization, yeast two-hybrid and BIFC further confirmed that the core components of ABA signaling pathways ZmPYL10 and ZmPP2C7 interacted in nucleus, ZmPP2C7 and ZmSRK2E as well as ZmSRK2E and ZmbZIP33 interacted in the plasma membrane. We also found that expression levels of ZmPYL10 and ZmSRK2E in the BMV-ZmbZIP33 mutant were lower than those of BMV, while ZmPP2C7 was the opposite under drought stress and rewatering. However, expression of ZmPYL10 and ZmSRK2E in normal maize leaves were significantly up-regulated by 3–4 folds after drought and ABA treatments for 24 h, while ZmPP2C7 was down-regulated. The NCED and ZEP encoding key enzymes in ABA biosynthesis are up-regulated in overexpression ZmbZIP33 transgenic line under drought stress and rewatering conditions, but down-regulated in BMV-ZmbZIP33 mutants. Together, these findings demonstrate that ZmbZIP33 played roles in ABA biosynthesis and regulation of drought response and rewatering in Arabidopsis and maize thought an ABA-dependent signaling pathway.

The R2R3 MYB transcription factor MYB189 negatively regulates secondary cell wall biosynthesis in Populus

2019 ◽  
Vol 39 (7) ◽  
pp. 1187-1200 ◽  
Author(s):  
Bo Jiao ◽  
Xin Zhao ◽  
Wanxiang Lu ◽  
Li Guo ◽  
Keming Luo
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Secondary Wall ◽  
Secondary Cell Wall ◽  
Populus Trichocarpa ◽  
Wood Formation ◽  
Myb Transcription Factor ◽  
Polymerase Chain ◽  
Xylem Fibers ◽  
R2r3 Myb

Abstract Secondary cell wall (SCW) biosynthesis during wood formation in trees is controlled by a multilevel regulatory network that coordinates the expression of substantial genes. However, few transcription factors involved in the negative regulation of secondary wall biosynthesis have been characterized in tree species. In this study, we isolated an R2R3 MYB transcription factor MYB189 from Populus trichocarpa, which is expressed predominantly in secondary vascular tissues, especially in the xylem. A novel repression motif was identified in the C-terminal region of MYB189, which indicates this factor was a transcriptional repressor. Overexpression (OE) of MYB189 in Arabidopsis and poplar resulted in a significant reduction in the contents of lignin, cellulose and hemicelluloses. Vascular development in stems of MYB189 OE lines was markedly inhibited, leading to a dramatic decrease in SCW thickness of xylem fibers. Gene expression analyses showed that most of the structural genes involved in the biosynthesis of lignin, cellulose and xylans were significantly downregulated in MYB189-overexpressing poplars compared with the wild-type control. Chromatin immunoprecipitation-quantitative real-time polymerase chain reaction and transient expression assays revealed that MYB189 could directly bind to the promoters of secondary wall biosynthetic genes to repress their expression. Together, these data suggest that MYB189 acts as a repressor to regulate SCW biosynthesis in poplar.

scholarly journals Partitioning of a Drought-induced Root Signal within the Fragaria chiloensis Plant

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 836G-837
Author(s):  
Douglas D. Archbold ◽  
Lailiang Cheng
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Water Relations ◽  
The Other ◽  
Pressure Potential ◽  
Xylem Exudate ◽  
Fragaria Chiloensis ◽  
Stress Signal ◽  
Root Signal ◽  
Aba Content

Plants of Fragaria chiloensis cv. RCP-37 were grown with their root system split between two separate containers. Water was withheld from one container of each pair (drought side), while the other was subirrigated. Control plants were subirrigated in both containers. Over several days, a drought-side leaf exhibited reductions in stomatal conductance (gs) and transpiration rate (T), while a subirrigated side leaf showed no change in either parameter. However, foliar water relations components (water, osmotic, and pressure potential) did not differ between the two leaves. The leaf on the subirrigated side exhibited gs, T, and water relations components similar to leaves on control plants. The abscisic acid (ABA) content of xylem exudate, collected from a stolon emerging from the axils of the measured leaves, was highest from the drought side and was negatively correlated to gs and T at some sampling dates. A root-derived drought stress signal, perhaps ABA, although other factors cannot be discounted, was limited within the plant to the drought side, even though water relations components indicated that water from the subirrigated side was allocated to all parts of the plant.

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