scholarly journals Studying SARS-CoV-2 with Fluorescence Microscopy

2021 ◽  
Vol 22 (12) ◽  
pp. 6558
Author(s):  
Lidia V. Putlyaeva ◽  
Konstantin A. Lukyanov
Keyword(s):  
Molecular Biology ◽  
Fluorescence Microscopy ◽  
Spatial Resolution ◽  
Super Resolution ◽  
High Specificity ◽  
Fluorescence Labeling ◽  
World Community ◽  
The World ◽  
Fundamental Research ◽  
Diagnostics And Therapy

The COVID-19 pandemic caused by SARS-CoV-2 coronavirus deeply affected the world community. It gave a strong impetus to the development of not only approaches to diagnostics and therapy, but also fundamental research of the molecular biology of this virus. Fluorescence microscopy is a powerful technology enabling detailed investigation of virus–cell interactions in fixed and live samples with high specificity. While spatial resolution of conventional fluorescence microscopy is not sufficient to resolve all virus-related structures, super-resolution fluorescence microscopy can solve this problem. In this paper, we review the use of fluorescence microscopy to study SARS-CoV-2 and related viruses. The prospects for the application of the recently developed advanced methods of fluorescence labeling and microscopy—which in our opinion can provide important information about the molecular biology of SARS-CoV-2—are discussed.

scholarly journals Three basic types of fluorescence microscopy and recent improvement

2021 ◽  
Vol 290 ◽  
pp. 01031
Author(s):  
Xinyi Wang ◽  
Yunyan Lai
Keyword(s):  
Confocal Microscopy ◽  
Molecular Biology ◽  
Fluorescence Microscopy ◽  
Spatial Resolution ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Future Perspectives ◽  
Recent Improvement ◽  
Basic Principles

Fluorescence microscopy is one of the most used imaging modalities in molecular biology and living specimens. To increase image contrast and spatial resolution, different type of fluorescence microscopy has been developed. This review introduces three main types of fluorescence microscopy: wild-field microscopy, confocal microscopy, and total internal reflection fluorescence microscopy. The basic principles are similar but with different modifications, which also indicates their attributes and limitation. The recent improvement on these microscopies is also discussed. Some most recent techniques show advance in overcoming common fluorescence microscopy's weakness, and future perspectives are also discussed.

High spatiotemporal resolution and low photo-toxicity fluorescence imaging in live cells and in vivo

2019 ◽  
Vol 47 (6) ◽  
pp. 1635-1650 ◽  
Author(s):  
Xiaohong Peng ◽  
Xiaoshuai Huang ◽  
Ke Du ◽  
Huisheng Liu ◽  
Liangyi Chen
Keyword(s):  
Fluorescence Microscopy ◽  
Spatial Resolution ◽  
Cell Biology ◽  
Critical Role ◽  
Super Resolution ◽  
Light Sheet ◽  
Live Cells ◽  
Spatiotemporal Resolution ◽  
And Function

Taking advantage of high contrast and molecular specificity, fluorescence microscopy has played a critical role in the visualization of subcellular structures and function, enabling unprecedented exploration from cell biology to neuroscience in living animals. To record and quantitatively analyse complex and dynamic biological processes in real time, fluorescence microscopes must be capable of rapid, targeted access deep within samples at high spatial resolutions, using techniques including super-resolution fluorescence microscopy, light sheet fluorescence microscopy, and multiple photon microscopy. In recent years, tremendous breakthroughs have improved the performance of these fluorescence microscopies in spatial resolution, imaging speed, and penetration. Here, we will review recent advancements of these microscopies in terms of the trade-off among spatial resolution, sampling speed and penetration depth and provide a view of their possible applications.

Near-field infrared nanospectroscopy and super-resolution fluorescence microscopy enable complementary nanoscale analyses of lymphocyte nuclei

The Analyst ◽  
2018 ◽  
Vol 143 (24) ◽  
pp. 5926-5934 ◽  
Author(s):  
Godwin C. Ajaezi ◽  
Max Eisele ◽  
Fabio Contu ◽  
Sadhana Lal ◽  
Aline Rangel-Pozzo ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Fluorescence Microscopy ◽  
Spatial Resolution ◽  
Nuclear Organization ◽  
Near Field ◽  
Super Resolution ◽  
Lymphocyte Nucleus

First near-field infrared spectroscopy and imaging of lymphocyte nucleus at 30 nm spatial resolution reveals spectrochemically distinct regions in nuclear organization.

Scanning x-ray fluorescence microscopy and principal component analysis

1992 ◽  
Vol 50 (2) ◽  
pp. 1752-1753
Author(s):  
Brian Cross
Keyword(s):  
Principal Component Analysis ◽  
Fluorescence Microscopy ◽  
Spatial Resolution ◽  
High Speed ◽  
Image Acquisition ◽  
Principal Component ◽  
Fluorescence Microscope ◽  
Acquisition Rate ◽  
X Ray ◽  
Speed Up

A relatively new entry, in the field of microscopy, is the Scanning X-Ray Fluorescence Microscope (SXRFM). Using this type of instrument (e.g. Kevex Omicron X-ray Microprobe), one can obtain multiple elemental x-ray images, from the analysis of materials which show heterogeneity. The SXRFM obtains images by collimating an x-ray beam (e.g. 100 μm diameter), and then scanning the sample with a high-speed x-y stage. To speed up the image acquisition, data is acquired "on-the-fly" by slew-scanning the stage along the x-axis, like a TV or SEM scan. To reduce the overhead from "fly-back," the images can be acquired by bi-directional scanning of the x-axis. This results in very little overhead with the re-positioning of the sample stage. The image acquisition rate is dominated by the x-ray acquisition rate. Therefore, the total x-ray image acquisition rate, using the SXRFM, is very comparable to an SEM. Although the x-ray spatial resolution of the SXRFM is worse than an SEM (say 100 vs. 2 μm), there are several other advantages.

TO THE QUESTION OF RESPONSIBILITY FOR TORTURE IN THE RUSSIAN FEDERATION

2020 ◽  
Vol 10 (2) ◽  
pp. 103-106
Author(s):  
ASTEMIR ZHURTOV ◽  
Keyword(s):  
Human Rights ◽  
Russian Federation ◽  
Large Scale ◽  
Human Life ◽  
International Standards ◽  
World Community ◽  
The World ◽  
Long Time ◽  
Protection Of Human Rights ◽  
The Russian Federation

Cruel and inhumane acts that harm human life and health, as well as humiliate the dignity, are prohibited in most countries of the world, and Russia is no exception in this issue. The article presents an analysis of the institution of responsibility for torture in the Russian Federation. The author comes to the conclusion that the current criminal law of Russia superficially and fragmentally regulates liability for torture, in connection with which the author formulated the proposals to define such act as an independent crime. In the frame of modern globalization, the world community pays special attention to the protection of human rights, in connection with which large-scale international standards have been created a long time ago. The Universal Declaration of Human Rights and other international acts enshrine prohibitions of cruel and inhumane acts that harm human life and health, as well as degrade the dignity.Considering the historical experience of the past, these standards focus on the prohibition of any kind of torture, regardless of the purpose of their implementation.

THE ROLE OF SOCIAL PARTNERSHIP IN THE DEVELOPMENT OF INCLUSIVE EDUCATION

2020 ◽  
Vol 7 (3) ◽  
pp. 81-88
Author(s):  
Enakhon Nishanbaeva ◽  
◽  
Abdulla Abdukhalilov
Keyword(s):  
Inclusive Education ◽  
Social Partnership ◽  
World Community ◽  
The World

Today, one of the most urgent tasks of the world community is the development of inclusive education. It should be noted that social partnership is of great importance in the development of inclusive education. Through social partnership, it is possible to establish constructive relations between various interested groups in the field of inclusive education.

International broadcasting: stages of development, specificity and functional structure

2020 ◽  
Vol 4 (4(13)) ◽  
pp. 51-60
Author(s):  
Ksenia Olegovna NEVMERZHITSKAYA ◽  
Keyword(s):  
Foreign Policy ◽  
Global Media ◽  
False Information ◽  
Broadcast Media ◽  
World Community ◽  
Media Agenda ◽  
International Broadcasting ◽  
The World ◽  
The Media ◽  
Media Reports

The media influence politics by providing intelligence and arena for political statements. Therefore, the danger of spreading false information and deliberate disinformation can have serious consequences. It is impossible to accuse specific media outlets of unfair coverage, but one cannot fail to note the existing resonance in media reports from different countries. Interpretations of the same events are radically different, while a journalist must rely on facts. The world is faced with the problem of global misunderstanding and information discord. Modern international broadcasting plays an important role in shaping the picture of the event for the world community. It is impossible to deny that the information agenda of many foreign broadcast media depends to some extent on a number of reasons: nationality, foreign policy of his state, profitability. Otherwise, the global media would not contradict each other. We want to track how modern foreign broadcasting builds its agenda and what principles it is guided by. Keywords: Broadcasting, media, Media agenda

scholarly journals Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 861
Author(s):  
Jacopo Cardellini ◽  
Arianna Balestri ◽  
Costanza Montis ◽  
Debora Berti
Keyword(s):  
Drug Delivery ◽  
Fluorescence Microscopy ◽  
Drug Delivery Systems ◽  
Laser Scanning ◽  
Super Resolution ◽  
Laser Scanning Confocal Microscopy ◽  
Delivery Systems ◽  
Scanning Confocal Microscopy ◽  
Biological Phenomena

In the past decade(s), fluorescence microscopy and laser scanning confocal microscopy (LSCM) have been widely employed to investigate biological and biomimetic systems for pharmaceutical applications, to determine the localization of drugs in tissues or entire organisms or the extent of their cellular uptake (in vitro). However, the diffraction limit of light, which limits the resolution to hundreds of nanometers, has for long time restricted the extent and quality of information and insight achievable through these techniques. The advent of super-resolution microscopic techniques, recognized with the 2014 Nobel prize in Chemistry, revolutionized the field thanks to the possibility to achieve nanometric resolution, i.e., the typical scale length of chemical and biological phenomena. Since then, fluorescence microscopy-related techniques have acquired renewed interest for the scientific community, both from the perspective of instrument/techniques development and from the perspective of the advanced scientific applications. In this contribution we will review the application of these techniques to the field of drug delivery, discussing how the latest advancements of static and dynamic methodologies have tremendously expanded the experimental opportunities for the characterization of drug delivery systems and for the understanding of their behaviour in biologically relevant environments.

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