scholarly journals Comparison of the Distinct, Host-Specific Response of Three Solanaceae Hosts Induced by Phytophthora infestans

2021 ◽  
Vol 22 (20) ◽  
pp. 11000
Author(s):  
Jie Lu ◽  
Tingli Liu ◽  
Xiong Zhang ◽  
Jie Li ◽  
Xun Wang ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Crop Production ◽  
Infection Process ◽  
Specific Response ◽  
Hormone Regulation ◽  
Host Interactions ◽  
Kegg Pathways ◽  
Early Expression ◽  
Infection Processes

Three Solanaceae hosts (TSHs), S. tuberosum, N. benthamiana and S. lycopersicum, represent the three major phylogenetic clades of Solanaceae plants infected by Phytophthora infestans, which causes late blight, one of the most devastating diseases seriously affecting crop production. However, details regarding how different Solanaceae hosts respond to P. infestans are lacking. Here, we conducted RNA-seq to analyze the transcriptomic data from the TSHs at 12 and 24 h post P. infestans inoculation to capture early expression effects. Macroscopic and microscopic observations showed faster infection processes in S. tuberosum than in N. benthamiana and S. lycopersicum under the same conditions. Analysis of the number of genes and their level of expression indicated that distinct response models were adopted by the TSHs in response to P. infestans. The host-specific infection process led to overlapping but distinct in GO terms and KEGG pathways enriched for differentially expressed genes; many were tightly linked to the immune response in the TSHs. S. tuberosum showed the fastest response and strongest accumulation of reactive oxygen species compared with N. benthamiana and S. lycopersicum, which also had similarities and differences in hormone regulation. Collectively, our study provides an important reference for a better understanding of late blight response mechanisms of different Solanaceae host interactions.

Discerning strains of Phytophthora infestans the causative agent of potato late blight by SSCP analysis of virulence genes

2018 ◽  
Vol 34 (6) ◽  
pp. 4-11
Author(s):  
V.K. Chizhik ◽  
◽  
E.A. Sokolova ◽  
V.V. Martynov ◽  
M.A. Kuznetsova ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Causative Agent ◽  
Virulence Genes ◽  
Potato Late Blight ◽  
Sscp Analysis

Evaluation of bio-agents and neem based products against late blight disease (Phytophthora infestans) of potato

2021 ◽  
Vol 74 (1) ◽  
pp. 181-187
Author(s):  
Mehi Lal ◽  
Sorabh Chaudhary ◽  
Sanjay Rawal ◽  
Sanjeev Sharma ◽  
Manoj Kumar ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Late Blight Disease ◽  
Blight Disease

scholarly journals Identification of the Dominant Genotypes of Phytophthora infestans in Canada Using Real-Time PCR with ASO-PCR Assays

Plant Disease ◽  
2016 ◽  
Vol 100 (7) ◽  
pp. 1482-1491 ◽  
Author(s):  
Marie-Claude Gagnon ◽  
Lawrence Kawchuk ◽  
D. Mathieu Tremblay ◽  
Odile Carisse ◽  
Giovanna Danies ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Rapid Assessment ◽  
Nucleotide Polymorphisms ◽  
Blind Test ◽  
Phytophthora Spp ◽  
Allele Specific ◽  
Flanking Sequences ◽  
Pcr Assays ◽  
And Control

Phytophthora infestans, a pathogenic oomycete that is the causal agent of potato and tomato late blight, has devastating effects worldwide. The genetic composition of P. infestans populations in Canada has changed considerably over the last few years, with the appearance of several new genotypes showing different mating types and sensitivity to the fungicide metalaxyl. Genetic markers allowing for a rapid assessment of genotypes from small amounts of biological material would be beneficial for the early detection and control of this pathogen throughout Canada. Mining of the P. infestans genome revealed several regions containing single-nucleotide polymorphisms (SNP) within both nuclear genes and flanking sequences of microsatellite loci. Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) assays were developed from 14 of the 50 SNP found by sequencing. Nine optimized ASO-PCR assays were validated using a blind test comprising P. infestans and other Phytophthora spp. The assays revealed diagnostic profiles unique to each of the five dominant genotypes present in Canada. The markers developed in this study can be used with environmental samples such as infected leaves, and will contribute to the genomic toolbox available to assess the genetic diversity of P. infestans at the intraspecific level. For late blight management, early warning about P. infestans genotypes present in potato and tomato fields will help growers select the most appropriate fungicides and application strategies.

scholarly journals Modeling of Yield Losses Caused by Potato Late Blight on Eight Cultivars with Different Levels of Resistance to Phytophthora infestans

Plant Disease ◽  
2012 ◽  
Vol 96 (7) ◽  
pp. 935-942 ◽  
Author(s):  
Toky Rakotonindraina ◽  
Jean-Éric Chauvin ◽  
Roland Pellé ◽  
Robert Faivre ◽  
Catherine Chatot ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Relative Yield ◽  
Weather Conditions ◽  
Potato Late Blight ◽  
Yield Losses ◽  
Predictive Quality ◽  
Wide Range ◽  
Cultivar Susceptibility

The Shtienberg model for predicting yield loss caused by Phytophthora infestans in potato was developed and parameterized in the 1990s in North America. The predictive quality of this model was evaluated in France for a wide range of epidemics under different soil and weather conditions and on cultivars different than those used to estimate its parameters. A field experiment was carried out in 2006, 2007, 2008, and 2009 in Brittany, western France to assess late blight severity and yield losses. The dynamics of late blight were monitored on eight cultivars with varying types and levels of resistance. The model correctly predicted relative yield losses (efficiency = 0.80, root mean square error of prediction = 13.25%, and bias = –0.36%) as a function of weather and the observed disease dynamics for a wide range of late blight epidemics. In addition to the evaluation of the predictive quality of the model, this article provides a dataset that describes the development of various late blight epidemics on potato as a function of weather conditions, fungicide regimes, and cultivar susceptibility. Following this evaluation, the Shtienberg model can be used with confidence in research and development programs to better manage potato late blight in France.

scholarly journals Complete mitogenome mapping of potato late blight pathogen, Phytophthora infestans A2 mating type

2017 ◽  
Vol 2 (1) ◽  
pp. 90-91
Author(s):  
Virupaksh U. Patil ◽  
G. Vanishree ◽  
Debasis Pattanayak ◽  
Sanjeev Sharma ◽  
Vinay Bhardwaj ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Mating Type ◽  
Potato Late Blight ◽  
Complete Mitogenome ◽  
Late Blight Pathogen

scholarly journals The 2009 Late Blight Pandemic in the Eastern United States – Causes and Results

Plant Disease ◽  
2013 ◽  
Vol 97 (3) ◽  
pp. 296-306 ◽  
Author(s):  
W. E. Fry ◽  
M. T. McGrath ◽  
A. Seaman ◽  
T. A. Zitter ◽  
A. McLeod ◽  
...  
Keyword(s):  
United States ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Current Situation ◽  
Causal Agent ◽  
Community Supported Agriculture ◽  
Eastern United States ◽  
Tomato Crop ◽  
History Of ◽  
Mainstream Press

The tomato late blight pandemic of 2009 made late blight into a household term in much of the eastern United States. Many home gardeners and many organic producers lost most if not all of their tomato crop, and their experiences were reported in the mainstream press. Some CSAs (Community Supported Agriculture) could not provide tomatoes to their members. In response, many questions emerged: How did it happen? What was unusual about this event compared to previous late blight epidemics? What is the current situation in 2012 and what can be done? It's easiest to answer these questions, and to understand the recent epidemics of late blight, if one knows a bit of the history of the disease and the biology of the causal agent, Phytophthora infestans.

scholarly journals Phenotypic Characterization of Recent Clonal Lineages of Phytophthora infestans in the United States

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 873-881 ◽  
Author(s):  
G. Danies ◽  
I. M. Small ◽  
K. Myers ◽  
R. Childers ◽  
W. E. Fry
Keyword(s):  
United States ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
The United States ◽  
Phenotypic Characterization ◽  
Phenotypic Traits ◽  
Mating Types ◽  
Late Blight Disease ◽  
Blight Disease

Phytophthora infestans, the causal agent of late blight disease, has been reported in the United States and Canada since the mid-nineteenth century. Due to the lack of or very limited sexual reproduction, the populations of P. infestans in the United States are primarily reproducing asexually and, thus, show a simple genetic structure. The emergence of new clonal lineages of P. infestans (US-22, US-23, and US-24) responsible for the late blight epidemics in the northeastern region of the United States in the summers of 2009 and 2010 stimulated an investigation into phenotypic traits associated with these genotypes. Mating type, differences in sensitivity to mefenoxam, differences in pathogenicity on potato and tomato, and differences in rate of germination were studied for clonal lineages US-8, US-22, US-23, and US-24. Both A1 and A2 mating types were detected. Lineages US-22, US-23, and US-24 were generally sensitive to mefenoxam while US-8 was resistant. US-8 and US-24 were primarily pathogenic on potato while US-22 and US-23 were pathogenic on both potato and tomato. Indirect germination was favored at lower temperatures (5 and 10°C) whereas direct germination, though uncommon, was favored at higher temperatures (20 and 25°C). Sporangia of US-24 released zoospores more rapidly than did sporangia of US-22 and US-23. The association of characteristic phenotypic traits with genotype enables the prediction of phenotypic traits from rapid genotypic analyses for improved disease management.

Resistance of tomatoes to late blight (Phytophthora infestans)

1970 ◽  
Vol 65 (2) ◽  
pp. 255-262 ◽  
Author(s):  
ELISABETH GÜNTHER ◽  
DÖRTHE EGGERT ◽  
G. GRÜMMER
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans
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