scholarly journals Polyamine Metabolism under Different Light Regimes in Wheat

2021 ◽  
Vol 22 (21) ◽  
pp. 11717
Author(s):  
Orsolya Kinga Gondor ◽  
Judit Tajti ◽  
Kamirán Áron Hamow ◽  
Imre Majláth ◽  
Gabriella Szalai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fine Tuning ◽  
Polyamine Metabolism ◽  
Light Conditions ◽  
Light Regimes ◽  
Test Light ◽  
Lower Light ◽  
The Relationship ◽  
Back Conversion ◽  
Gene Expression Levels

Although the relationship between polyamines and photosynthesis has been investigated at several levels, the main aim of this experiment was to test light-intensity-dependent influence of polyamine metabolism with or without exogenous polyamines. First, the effect of the duration of the daily illumination, then the effects of different light intensities (50, 250, and 500 μmol m–2 s–1) on the polyamine metabolism at metabolite and gene expression levels were investigated. In the second experiment, polyamine treatments, namely putrescine, spermidine and spermine, were also applied. The different light quantities induced different changes in the polyamine metabolism. In the leaves, light distinctly induced the putrescine level and reduced the 1,3-diaminopropane content. Leaves and roots responded differently to the polyamine treatments. Polyamines improved photosynthesis under lower light conditions. Exogenous polyamine treatments influenced the polyamine metabolism differently under individual light regimes. The fine-tuning of the synthesis, back-conversion and terminal catabolism could be responsible for the observed different polyamine metabolism-modulating strategies, leading to successful adaptation to different light conditions.

scholarly journals H3 K79 dimethylation marks developmental activation of the β-globin gene but is reduced upon LCR-mediated high-level transcription

Blood ◽  
2008 ◽  
Vol 112 (2) ◽  
pp. 406-414 ◽  
Author(s):  
Tomoyuki Sawado ◽  
Jessica Halow ◽  
Hogune Im ◽  
Tobias Ragoczy ◽  
Emery H. Bresnick ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Activation ◽  
Globin Gene ◽  
Erythroid Differentiation ◽  
Genome Wide ◽  
Activation Of Transcription ◽  
High Level ◽  
The Relationship ◽  
Mutant Genes ◽  
Gene Expression Levels

Abstract Genome-wide analyses of the relationship between H3 K79 dimethylation and transcription have revealed contradictory results. To clarify this relationship at a single locus, we analyzed expression and H3 K79 modification levels of wild-type (WT) and transcriptionally impaired β-globin mutant genes during erythroid differentiation. Analysis of fractionated erythroid cells derived from WT/Δ locus control region (LCR) heterozygous mice reveals no significant H3 K79 dimethylation of the β-globin gene on either allele prior to activation of transcription. Upon transcriptional activation, H3 K79 di-methylation is observed along both WT and ΔLCR alleles, and both alleles are located in proximity to H3 K79 dimethylation nuclear foci. However, H3 K79 di-methylation is significantly increased along the ΔLCR allele compared with the WT allele. In addition, analysis of a partial LCR deletion mutant reveals that H3 K79 dimethylation is inversely correlated with β-globin gene expression levels. Thus, while our results support a link between H3 K79 dimethylation and gene expression, high levels of this mark are not essential for high level β-globin gene transcription. We propose that H3 K79 dimethylation is destabilized on a highly transcribed template.

scholarly journals Network analysis reveals the relationship among wood properties, gene expression levels and genotypes of naturalPopulus trichocarpaaccessions

2013 ◽  
Vol 200 (3) ◽  
pp. 727-742 ◽  
Author(s):  
Ilga Porth ◽  
Jaroslav Klápště ◽  
Oleksandr Skyba ◽  
Michael C. Friedmann ◽  
Jan Hannemann ◽  
...  
Keyword(s):  
Gene Expression ◽  
Network Analysis ◽  
Wood Properties ◽  
Expression Levels ◽  
The Relationship ◽  
Gene Expression Levels

scholarly journals Experimental Hyperthyroidism Decreases Gene Expression and Serum Levels of Adipokines in Obesity

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Renata de Azevedo Melo Luvizotto ◽  
André Ferreira do Nascimento ◽  
Maria Teresa de Síbio ◽  
Regiane Marques Castro Olímpio ◽  
Sandro José Conde ◽  
...  
Keyword(s):  
Gene Expression ◽  
Weight Loss ◽  
Adipose Tissue ◽  
Serum Concentration ◽  
Serum Levels ◽  
Fat Deposits ◽  
Ad Libitum ◽  
The Relationship ◽  
Hypercaloric Diet ◽  
Gene Expression Levels

Aims. To analyze the influence of hyperthyroidism on the gene expression and serum concentration of leptin, resistin, and adiponectin in obese animals.Main Methods. MaleWistarrats were randomly divided into two groups: control (C)—fed with commercial chow ad libitum—and obese (OB)—fed with a hypercaloric diet. After group characterization, the OB rats continued receiving a hypercaloric diet and were randomized into two groups: obese animals (OB) and obese with 25 μg triiodothyronine (T3)/100 BW (OT). The T3dose was administered every day for the last 2 weeks of the study. After 30 weeks the animals were euthanized. Samples of blood and adipose tissue were collected for biochemical and hormonal analyses as well as gene expression of leptin, resistin, and adiponectin.Results. T3treatment was effective, increasing fT3levels and decreasing fT4and TSH serum concentration. Administration of T3promotes weight loss, decreases all fat deposits, and diminishes serum levels of leptin, resistin, and adiponectin by reducing their gene expression.Conclusions. Our results suggest that T3modulate serum and gene expression levels of leptin, resistin, and adiponectin in experimental model of obesity, providing new insights regarding the relationship between T3and adipokines in obesity.

Dietary iron alters waterborne copper-induced gene expression in soft water acclimated zebrafish (Danio rerio)

2009 ◽  
Vol 296 (2) ◽  
pp. R362-R373 ◽  
Author(s):  
Paul M. Craig ◽  
Michal Galus ◽  
Chris M. Wood ◽  
Grant B. McClelland
Keyword(s):  
Gene Expression ◽  
Protein Function ◽  
Interactive Effects ◽  
Protein Abundance ◽  
Copper Transporter ◽  
Metal Transporter ◽  
Protein Levels ◽  
The Relationship ◽  
Cu Uptake ◽  
Gene Expression Levels

Metals like iron (Fe) and copper (Cu) function as integral components in many biological reactions, and, in excess, these essential metals are toxic, and organisms must control metal acquisition and excretion. We examined the effects of chronic waterborne Cu exposure and the interactive effects of elevated dietary Fe on gene expression and tissue metal accumulation in zebrafish. Softwater acclimated zebrafish exposed to 8 μg/l Cu, with and without supplementation of a diet high in Fe (560 vs. 140 mg Fe/kg food) for 21 days demonstrated a significant reduction in liver and gut Cu load relative to waterborne Cu exposure alone. Gene expression levels for divalent metal transport (DMT)-1, copper transporter (CTR)-1, and the basolateral metal transporter ATP7A in the gills and gut increased when compared with controls, but the various combinations of Cu and high-Fe diet revealed altered levels of expression. Further examination of the basolateral Fe transporter, ferroportin, showed responses to waterborne Cu exposure in the gut and a significant increase with Fe treatment alone in the liver. Additionally, we examined metallothionein 1 and 2 (MT1 and MT2), which indicated that MT2 is more responsive to Cu. To explore the relationship between transcription and protein function, we examined both CTR-1 protein levels and gill apical uptake of radiolabeled Cu64, which demonstrated decreased Cu uptake and protein abundance in the elevated Cu treatments. This study shows that high dietary Fe can significantly alter the genetic expression pattern of Cu transporters at the level of the gill, liver, and gastrointestinal tract.

The relationship between proangiogenic gene expression levels in prostate cancer and their prognostic value for clinical outcomes

The Prostate ◽  
2010 ◽  
Vol 70 (15) ◽  
pp. 1692-1700 ◽  
Author(s):  
Ryutaro Mori ◽  
Tanya B. Dorff ◽  
Shigang Xiong ◽  
Chad J. Tarabolous ◽  
Wei Ye ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Clinical Outcomes ◽  
Prognostic Value ◽  
Expression Levels ◽  
The Relationship ◽  
Gene Expression Levels

Association between Quantitative MR Markers of Cortical Evolving Organization and Gene Expression during Human Prenatal Brain Development

2021 ◽  
Author(s):  
Lana Vasung ◽  
Chenying Zhao ◽  
Matthew Barkovich ◽  
Caitlin K Rollins ◽  
Jennings Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Structural Changes ◽  
Vascular Malformations ◽  
Fetal Brain ◽  
Cortical Plate ◽  
Expression Levels ◽  
Quantitative Mr ◽  
Magnetic Resonance Imaging Mri ◽  
The Relationship ◽  
Gene Expression Levels

Abstract The relationship between structural changes of the cerebral cortex revealed by Magnetic Resonance Imaging (MRI) and gene expression in the human fetal brain has not been explored. In this study, we aimed to test the hypothesis that relative regional thickness (a measure of cortical evolving organization) of fetal cortical compartments (cortical plate [CP] and subplate [SP]) is associated with expression levels of genes with known cortical phenotype. Mean regional SP/CP thickness ratios across age measured on in utero MRI of 25 healthy fetuses (20–33 gestational weeks [GWs]) were correlated with publicly available regional gene expression levels (23–24 GW fetuses). Larger SP/CP thickness ratios (more pronounced cortical evolving organization) was found in perisylvian regions. Furthermore, we found a significant association between SP/CP thickness ratio and expression levels of the FLNA gene (mutated in periventricular heterotopia, congenital heart disease, and vascular malformations). Further work is needed to identify early MRI biomarkers of gene expression that lead to abnormal cortical development.

RELATIONSHIP BETWEEN CELL FUNCTION AND INITIAL CELL SEEDING DENSITY OF PRIMARY PORCINE CHONDROCYTES IN VITRO

2013 ◽  
Vol 25 (05) ◽  
pp. 1340001 ◽  
Author(s):  
Kun Zhang ◽  
Lili Wang ◽  
Qianqian Han ◽  
Boon Chin Heng ◽  
Zheng Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Function ◽  
Seeding Density ◽  
Cell Seeding ◽  
Large Numbers ◽  
The Relationship ◽  
Gene Expression Levels ◽  
Functional Phenotype ◽  
Cultured Chondrocytes

Maintenance of differentiated functional phenotype within in vitro chondrocyte culture requires seeding at high densities with large numbers of cells. However, optimal cell seeding numbers and densities remain elusive due to multiple varying parameters and different methodologies utilized in previous studies. In the current study, we tried to investigate the relationship between cell seeding number and differentiated functional phenotype of in vitro cultured chondrocytes. Varying numbers of primary porcine chondrocytes (0.25, 2.5, 25 and 250 K) were seeded in 96 well-plates and cultured for 4 weeks. Cell proliferation, glycosaminoglycan (GAG) production and gene expression levels of Sox9, aggrecan, COL II and COL I were evaluated. The results showed that GAG content was high in the 0.25 and 25 K groups, gene expression of Sox9 was high in the 2.5, 25 and 250 K groups and expression of COL II was high in the 25 K group, whereas expression of COL I was low in the 0.25, 25 and 250 K groups. It is concluded that the seeding number and density of the 25 K (78 K cells/cm2) group achieved the optimal balance between functional phenotype of individual cells and the total ECM production for in vitro cultured chondrocytes.

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