Genome-Wide Analysis of the Soybean Calmodulin-Binding Protein 60 Family and Identification of GmCBP60A-1 Responses to Drought and Salt Stresses

Calmodulin-binding protein 60 (CBP60) members constitute a plant-specific protein family that plays an important role in plant growth and development. In the soybean genome, nineteen CBP60 members were identified and analyzed for their corresponding sequences and structures to explore their functions. Among GmCBP60A-1, which primarily locates in the cytomembrane, was significantly induced by drought and salt stresses. The overexpression of GmCBP60A-1 enhanced drought and salt tolerance in Arabidopsis, which showed better state in the germination of seeds and the root growth of seedlings. In the soybean hairy roots experiment, the overexpression of GmCBP60A-1 increased proline content, lowered water loss rate and malondialdehyde (MDA) content, all of which likely enhanced the drought and salt tolerance of soybean seedlings. Under stress conditions, drought and salt response-related genes showed significant differences in expression in hairy root soybean plants of GmCBP60A-1-overexpressing and hairy root soybean plants of RNAi. The present study identified GmCBP60A-1 as an important gene in response to salt and drought stresses based on the functional analysis of this gene and its potential underlying mechanisms in soybean stress-tolerance.

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A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher K+/Na+ Ratio in Soybean

Frontiers in Plant Science ◽

10.3389/fpls.2021.638340 ◽

2021 ◽

Vol 12 ◽

Author(s):

Tianjie Sun ◽

Nan Ma ◽

Caiqing Wang ◽

Huifen Fan ◽

Mengxuan Wang ◽

...

Keyword(s):

Salt Stress ◽

Salt Tolerance ◽

Soil Salinization ◽

Tolerance Mechanism ◽

Salt Treatment ◽

Soybean Seedlings ◽

Yield And Quality ◽

Sodium Hydrogen ◽

Sodium Hydrogen Exchanger ◽

Soybean Plants

Salt stress caused by soil salinization, is one of the main factors that reduce soybean yield and quality. A large number of genes have been found to be involved in the regulation of salt tolerance. In this study, we characterized a soybean sodium/hydrogen exchanger gene GmNHX5 and revealed its functional mechanism involved in the salt tolerance process in soybean. GmNHX5 responded to salt stress at the transcription level in the salt stress-tolerant soybean plants, but not significantly changed in the salt-sensitive ones. GmNHX5 was located in the Golgi apparatus, and distributed in new leaves and vascular, and was induced by salt treatment. Overexpression of GmNHX5 improved the salt tolerance of hairy roots induced by soybean cotyledons, while the opposite was observed when GmNHX5 was knockout by CRISPR/Cas9. Soybean seedlings overexpressing GmNHX5 also showed an increased expression of GmSOS1, GmSKOR, and GmHKT1, higher K+/Na+ ratio, and higher viability when exposed to salt stress. Our findings provide an effective candidate gene for the cultivation of salt-tolerant germplasm resources and new clues for further understanding of the salt-tolerance mechanism in plants.

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The WRKY Transcription Factor GmWRKY12 Confers Drought and Salt Tolerance in Soybean

International Journal of Molecular Sciences ◽

10.3390/ijms19124087 ◽

2018 ◽

Vol 19 (12) ◽

pp. 4087 ◽

Cited By ~ 21

Author(s):

Wen-Yan Shi ◽

Yong-Tao Du ◽

Jian Ma ◽

Dong-Hong Min ◽

Long-Guo Jin ◽

...

Keyword(s):

Abiotic Stress ◽

Salt Tolerance ◽

Stress Responses ◽

Abiotic Stress Tolerance ◽

Wrky Transcription Factor ◽

Rna Seq ◽

Soybean Seedlings ◽

Drought And Salt Tolerance ◽

Drought And Salt Stress ◽

Mda Content

WRKYs are important regulators in plant development and stress responses. However, knowledge of this superfamily in soybean is limited. In this study, we characterized the drought- and salt-induced gene GmWRKY12 based on RNA-Seq and qRT-PCR. GmWRKY12, which is 714 bp in length, encoded 237 amino acids and grouped into WRKY II. The promoter region of GmWRKY12 included ABER4, MYB, MYC, GT-1, W-box and DPBF cis-elements, which possibly participate in abscisic acid (ABA), drought and salt stress responses. GmWRKY12 was minimally expressed in different tissues under normal conditions but highly expressed under drought and salt treatments. As a nucleus protein, GmWRKY12 was responsive to drought, salt, ABA and salicylic acid (SA) stresses. Using a transgenic hairy root assay, we further characterized the roles of GmWRKY12 in abiotic stress tolerance. Compared with control (Williams 82), overexpression of GmWRKY12 enhanced drought and salt tolerance, increased proline (Pro) content and decreased malondialdehyde (MDA) content under drought and salt treatment in transgenic soybean seedlings. These results may provide a basis to understand the functions of GmWRKY12 in abiotic stress responses in soybean.

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Overexpression of Auxin Binding Protein 57 from Rice (Oryza sativa L.) Increased Drought and Salt Tolerance in Transgenic Arabidopsis thaliana

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/197/1/012038 ◽

2018 ◽

Vol 197 ◽

pp. 012038

Author(s):

L W Tan ◽

C S Tan ◽

A R Zuraida ◽

H M Hossein ◽

H H Goh ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Oryza Sativa ◽

Salt Tolerance ◽

Transgenic Arabidopsis ◽

Oryza Sativa L ◽

Binding Protein ◽

Transgenic Arabidopsis Thaliana ◽

Drought And Salt Tolerance ◽

Auxin Binding ◽

Auxin Binding Protein

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A 115 kDa calmodulin-binding protein is located in rat liver endosome fractions

Biochemical Journal ◽

10.1042/bj2550999 ◽

1988 ◽

Vol 255 (3) ◽

pp. 999-1005 ◽

Cited By ~ 14

Author(s):

C Enrich ◽

O Bachs ◽

W H Evans

Keyword(s):

Plasma Membrane ◽

Rat Liver ◽

Plasma Membranes ◽

Binding Protein ◽

Specific Protein ◽

Subcellular Fractions ◽

Calmodulin Binding ◽

Overlay Technique ◽

Protein Partitioning ◽

Triton X

The distribution of calmodulin-binding polypeptides in various rat liver subcellular fractions was investigated. Plasma-membrane, endosome, Golgi and lysosome fractions were prepared by established procedures. The calmodulin-binding polypeptides present in the subcellular fractions were identified by using an overlay technique after transfer from gels to nitrocellulose sheets. Distinctive populations of calmodulin-binding polypeptides were present in all the fractions examined except lysosomes. A major 115 kDa calmodulin-binding polypeptide of pI 4.3 was located to the endosome subfractions, and it emerges as a candidate endosome-specific protein. Partitioning of endosome fractions between aqueous and Triton X-114 phases indicated that the calmodulin-binding polypeptide was hydrophobic. Major calmodulin-binding polypeptides of 140 and 240 kDa and minor polypeptides of 40-60 kDa were present in plasma membranes. The distribution of calmodulin in the various endosome and plasma-membrane fractions was also analysed, and the results indicated that the amounts were high compared with those in the cytosol.

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