scholarly journals Molecular Cloning and Expression Profiles of Thermosensitive TRP Genes in Agasicles hygrophila

Insects ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 531
Author(s):  
Dong Jia ◽  
Zhouyu Ji ◽  
Xiaofang Yuan ◽  
Bin Zhang ◽  
Yanhong Liu ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Temperature Sensing ◽  
Cloning And Expression ◽  
Alternanthera Philoxeroides ◽  
Control Efficacy ◽  
Invasive Weed ◽  
Preferred Temperature ◽  
Cdna Sequences ◽  
Temporal Expressions ◽  
Trp Genes

Global warming has gradually reduced the control efficacy of Agasicles hygrophila against the invasive weed Alternanthera philoxeroides. To better understand the summer collapse of A. hygrophila populations, we cloned the cDNA sequences of the high temperature-sensing TRPA1, Painless, and Pyrexia from A. hygrophila, and analyzed their temporal expressions and the impacts of high temperatures on their expression in eggs, the most vulnerable stage of A. hygrophila to hot temperatures. All the three genes obtained had the signature domains of TRPA channels and were constitutively expressed in eggs, larvae (L1, L2, L3), pupae, and adults, but AhPainless had the highest expression, followed by AhPyrexia, and AhTRPA1. The lowest and highest expression stages were adult and pupae for AhTRPA1, egg and L3 for AhPainless, and pupae/adult and L2 for AhPyrexia. The expressions of AhTRPA1, AhPainless, and AhPyrexia remained low at the preferred temperature range of 25–28 °C, elevated to their peak levels at 37.5, 30, and 30 °C, respectively, then fell to their 25–28 °C levels (AhTRPA1, AhPainless) or a lower level (AhPyrexia) at one or more temperatures >30 or 37.5 °C. These results suggest that their temperature-sensing roles and importance may be different, stage-specific, and their expression may be decoupled from their activation.

scholarly journals Gene Expression Profiling Reveals Enhanced Defense Responses in an Invasive Weed Compared to Its Native Congener during Pathogenesis

2019 ◽  
Vol 20 (19) ◽  
pp. 4916 ◽  
Author(s):  
Manoharan ◽  
Qi ◽  
Dhandapani ◽  
Chen ◽  
Rutherford ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Invasive Plants ◽  
Cross Talk ◽  
Expression Profiles ◽  
Defense Responses ◽  
Alternanthera Philoxeroides ◽  
Disease Development ◽  
Invasive Weed

Invasive plants are a huge burden on the environment, and modify local ecosystems by affecting the indigenous biodiversity. Invasive plants are generally less affected by pathogens, although the underlying molecular mechanisms responsible for their enhanced resistance are unknown. We investigated expression profiles of three defense hormones (salicylic acid, jasmonic acid, and ethylene) and their associated genes in the invasive weed, Alternanthera philoxeroides, and its native congener, A. sessilis, after inoculation with Rhizoctonia solani. Pathogenicity tests showed significantly slower disease progression in A. philoxeroides compared to A. sessilis. Expression analyses revealed jasmonic acid (JA) and ethylene (ET) expressions were differentially regulated between A. philoxeroides and A. sessilis, with the former having prominent antagonistic cross-talk between salicylic acid (SA) and JA, and the latter showing weak or no cross-talk during disease development. We also found that JA levels decreased and SA levels increased during disease development in A. philoxeroides. Variations in hormonal gene expression between the invasive and native species (including interspecific differences in the strength of antagonistic cross-talk) were identified during R. solani pathogenesis. Thus, plant hormones and their cross-talk signaling may improve the resistance of invasive A. philoxeroides to pathogens, which has implications for other invasive species during the invasion process.

The full-length cDNA cloning and expression profiles of 14-3-3 genes from the mud crab Scylla paramamosain Estampador, 1949

Crustaceana ◽  
2015 ◽  
Vol 88 (10-11) ◽  
pp. 1065-1078 ◽  
Author(s):  
Miao-An Shu ◽  
Chen Long ◽  
Wei-Ren Dong ◽  
Pan Zhang ◽  
Bin-Peng Xu ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Expression Patterns ◽  
Full Length ◽  
Cloning And Expression ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Phylogenetic Tree Analysis ◽  
Specific Expression ◽  
Full Length Cdna ◽  
Cdna Sequences

Two full-length cDNA sequences of 14-3-3 genes were isolated from the mud crab, Scylla paramamosain Estampador, 1949, using RT-PCR and RACE (rapid-amplification of cDNA ends). Sequence analysis indicates that both 14-3-3 genes contain an open reading frame of 744 bp with a deduced 247-amino-acid protein. The gene and protein sequences of Sp14-3-3 genes show 96 and 97% identity, respectively, and both Sp14-3-3 cluster together with other animal 14-3-3 proteins in phylogenetic tree analysis. Tissue specific expression analysis reveals that both Sp14-3-3 genes are ubiquitously expressed, however, their expression patterns are different from each other. Expressions of both Sp14-3-3 genes are sensitive to salinity decrease, even a 5 ppt drop from 30 ppt to 25 ppt, indicating that Sp14-3-3 genes are involved in osmoregulation. Our observations may contribute to a better understanding of the molecular and functional evolution of the 14-3-3 family in both crustaceans and (in)vertebrates as a whole.

Evaluation of Herbicide Options for Alligatorweed (Alternanthera philoxeroides) Control in Rice

2015 ◽  
Vol 29 (4) ◽  
pp. 793-799 ◽  
Author(s):  
Samuel D. Willingham ◽  
Muthukumar V. Bagavathiannan ◽  
Katherine S. Carson ◽  
Todd J. Cogdill ◽  
Garry N. McCauley ◽  
...  
Keyword(s):  
United States ◽  
Weed Control ◽  
Field Studies ◽  
Rice Production ◽  
Alternanthera Philoxeroides ◽  
Southern United States ◽  
Rice Grain ◽  
Effective Management ◽  
Invasive Weed ◽  
Antagonistic Interactions

Alligatorweed is a perennial, invasive weed in southern United States rice production, but knowledge on effective management of this weed is limited, especially in conventional (non-imidazolinone-resistant) rice fields. Field studies were conducted in multiple environments in southeastern Texas to evaluate different herbicide options involving penoxsulam, propanil, triclopyr, halosulfuron, bispyribac-sodium, bensulfuron, and quinclorac for alligatorweed control in conventional drill-seeded rice when applied at early POST (EPOST), late POST (LPOST), or both. Among the herbicide options evaluated, penoxsulam alone (up to 83%), penoxsulam plus triclopyr (up to 87%), or bispyribac-sodium plus triclopyr (92%) provided superior alligatorweed control. Plots treated with penoxsulam plus triclopyr EPOST produced the highest yields (9,550 kg ha−1), which were comparable to plots receiving penoxsulam plus triclopyr LPOST (9,320 kg ha−1), penoxsulam alone EPOST (9,280 kg ha−1), and penoxsulam plus halosulfuron LPOST (9,180 kg ha−1). Considering both weed control and rice grain yields, penoxsulam plus triclopyr applied EPOST was found to be the best option among the treatments tested. The treatments bensulfuron alone, bensulfuron plus propanil, penoxsulam plus propanil, triclopyr plus propanil, and bispyribac-sodium plus propanil provided poor (≤ 65%) alligatorweed control. Results also suggest the likelihood for antagonistic interactions when tank-mix combinations tested in this study included propanil.

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

2010 ◽  
Vol 40 (4) ◽  
pp. 217-224 ◽  
Author(s):  
Rye Jin KIM ◽  
Seung Han OH ◽  
Mi Young NOH ◽  
Yong Hun JO ◽  
Seongon KIM ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Plutella Xylostella ◽  
Expression Profiles ◽  
Diamondback Moth ◽  
Cloning And Expression

Cloning and expression profiles of tumor suppressor QM homologue in response to granulovirus in Pieris rapae

2011 ◽  
Vol 41 (6) ◽  
pp. 293-293
Author(s):  
Yong Hun JO ◽  
Seung Han OH ◽  
Bo Mi PARK ◽  
Dong Hyun Kim ◽  
Bharat Bhusan PATNAIK ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Pieris Rapae ◽  
Expression Profiles ◽  
Cloning And Expression

scholarly journals Molecular cloning and expression profile of β-ketoacyl-acp synthase gene from tung tree (Vernicia fordii Hemsl.)

Genetika ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 143-159 ◽  
Author(s):  
Hongxu Long ◽  
Xiaofeng Tan ◽  
Fangfang Yan ◽  
Lin Zhang ◽  
Ze Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Full Length ◽  
Cloning And Expression ◽  
Tung Tree ◽  
Degenerate Primers ◽  
Expression Levels ◽  
Full Length Cdna ◽  
Vernicia Fordii

Tung tree (Vernicia fordii) is an important woody oil tree. Tung tree seeds contain 50-60% oil with approximately 80 mole ?-eleostearic acid (9 cis, 11 trans, 13 trans octadecatrienoic acid). Fatty acid synthesis is catalyzed by the concerted action of acetyl-CoA carboxylase and fatty acid synthase, a multienzyme complex including ?-ketoacyl-acyl-carrier-protein synthase (KAS). Little is known about KAS in tung tree. The objective of this study was to clone KAS genes and analyze their expression profiles in tung tree. A full-length cDNA encoding KAS III and a partial cDNA encoding KAS II were isolated from tung tree by PCR cloning using degenerate primers and rapid amplification of cDNA ends system. The full-length cDNA of VfKAS III was 1881 bp in length with an open reading frame of 1212 bp. VfKAS III genomic DNA was also isolated and sequenced, which contained 8 exons in 5403 bp length. The deduced VfKAS III protein shared approximately 80% identity with homologous KAS IIIs from other plants. Quantitative PCR analysis revealed that KAS II and KAS III were expressed in all of the tissues and organs tested but exhibited different expression patterns in tung tree. The expression levels of KAS II in young tissues were much lower than those in mature tissues, whereas the highest expression levels of KAS III were observed in young stem and young leaf. These results should facilitate further studies on the regulation of tung oil biosynthesis by KAS in tung tree.

scholarly journals Molecular Cloning and Expression of MnGST-1 and MnGST-2 from Oriental River Prawn, Macrobrachium nipponense, in Response to Hypoxia and Reoxygenation

2018 ◽  
Vol 19 (10) ◽  
pp. 3102 ◽  
Author(s):  
Lei Xu ◽  
Ming Yang ◽  
Hongtuo Fu ◽  
Shengming Sun ◽  
Hui Qiao ◽  
...  
Keyword(s):  
Intracellular Transport ◽  
Expression Profiles ◽  
Acute Hypoxia ◽  
Cloning And Expression ◽  
Glutathione S Transferase ◽  
Specific Expression ◽  
Macrobrachium Nipponense ◽  
Hypoxia Stress ◽  
Oriental River Prawn ◽  
Specific Expression Pattern

The glutathione-S-transferase (GST) superfamily includes seven classes, and different classes have different functions. GST superfamily members function in various processes including detoxification of xenobiotics, protection against oxidative damage, and intracellular transport of hormones, endogenous metabolites, and exogenous chemicals. Herein, to elucidate the tissue-specific expression pattern of GSTs in response to hypoxia stress, which induces cell death, we investigated the expression of GSTs in response to hypoxia and reoxygenation in oriental river prawn, Macrobrachium nipponense. Full-length cDNAs of two δ class GSTs were cloned from the hepatopancreas, and named MnGST-1 and MnGST-2 based on the established GST nomenclature system. Expression profiles of both GSTs in various tissues were different under acute and chronic experimental hypoxia stress conditions, suggesting that both respond strongly to hypoxia-induced oxidative stress. However, the intensity of responses to hypoxia and reoxygenation were different in different tissues. During acute hypoxia stress, MnGST-1 responds earlier than MnGST-2 in the hepatopancreas and gill, but more slowly in muscle. By contrast, during chronic hypoxia stress, MnGST-2 plays a more important role in the hepatopancreas and gill than MnGST-1.

scholarly journals Identification and preliminary characterization of chemosensory-related proteins in the gall fly, Procecidochares utilis by transcriptomic analysis

2020 ◽  
Author(s):  
lifang Li ◽  
Xi Gao ◽  
Huamin Gui ◽  
Mingxian Lan ◽  
Jiaying Zhu ◽  
...  
Keyword(s):  
Biological Control ◽  
Differentially Expressed Genes ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Invasive Weed ◽  
Male Adult ◽  
Next Generation Sequencing Technology ◽  
Chemosensory Genes ◽  
Stem Gall

Abstract Background: Chemoreception is critical for insect behaviors such as foraging, host searching and oviposition, and finding mating partners. The process of chemoreception is mediated by a series of proteins, including odorant-binding proteins (OBPs), gustatory receptors (GRs), odorant receptors (ORs), ionotropic receptors (IRs), chemosensory proteins (CSPs) and sensory neuron membrane proteins (SNMPs). The tephritid stem gall fly, Procecidochares utilis Stone, is a type of egg parasitic insect, which is an effective biological control reagent for the invasive weed Ageratina adenophora in many countries. However, the study of molecular components related to the olfactory system of P. utilis has not been investigated. Here, we report the developmental transcriptome (egg, first-third instar larvae, pupae, fame and male adult) of P. utilis using next-generation sequencing technology and identified the major chemosensory related genes.Results: In this study, a total of 133 chemosensory genes in P. utilis transcriptomes were identified by bioinformatics analysis, including 40 OBPs, 29 GRs, 24 ORs, 28 IRs, 6 CSPs, and 6 SNMPs in P. utilis. The sequences of these candidate chemosensory genes were confirmed by BLAST, and phylogenetic analysis was performed. The expression profiles of all candidate genes at different developmental stages were analyzed by differentially expressed genes (DEGs) analysis and then the expression profiles of the OBPs in the seven developmental stages were confirmed by real-time quantitative RT-PCR (qPCR). The results showed that the expression of candidate OBP genes across different developmental stages was consistent with the differentially expressed genes (DEGs) analysis using the fragments per kilobase per million fragments (FPKM) value.Conclusions: A large number of chemosensory genes were identified. This study will provide a significant contribution to the molecular mechanism of chemoreception and help advance the use of P. utilis as biological control agents.

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