Evaluation of Vascular Endothelial Function in Children with Type 1 Diabetes Mellitus

Diabetic kidney disease belongs to the major complications of diabetes mellitus. Here, hyperglycaemia is a key metabolic factor that causes endothelial dysfunction and vascular changes within the renal glomerulus. The aim of the present study was to assess the function of the vascular endothelium in children with type 1 diabetes mellitus (type 1 diabetes) by measuring selected endothelial lesion markers in blood serum. The selected markers of endothelial lesions (sVCAM-1, sICAM-1, sE-SELECTIN, PAI-1, ADMA and RAGE) were assayed by the immunoenzymatic ELISA method. The study involved 66 patients (age: 5–18 years) with type 1 diabetes and 21 healthy controls (age: 5–16 years). In the type 1 diabetes patients, significantly higher concentrations of all of the assayed markers were observed compared to the healthy controls (p < 0.001). All of the evaluated markers positively correlated with the disease duration, the age, and BMI of the patients, while only PAI-1 and sE-SELECTIN were characteristic of linear correlations with the estimated glomerular filtration rate (eGFR). It can be concluded that endothelial inflammatory disease occurs in the early stages of type 1 diabetes mellitus in children. The correlations between PAI-1, sE-SELECTIN, and eGFR suggest an advantage of these markers over other markers of endothelial dysfunction as prognostic factors for kidney dysfunction in children with type 1 diabetes.

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Levels of 25(OH)vitamin D in children and adolescents with type 1 diabetes mellitus and in healthy controls in Bulgarian population

Bone Abstracts ◽

10.1530/boneabs.2.p199 ◽

2013 ◽

Author(s):

Slavcheva Olga ◽

Konstantinova Maia ◽

Tsekova Adelina ◽

Savova Radka ◽

Arshinkova Margarita

Keyword(s):

Diabetes Mellitus ◽

Vitamin D ◽

Type 1 Diabetes ◽

Type 1 Diabetes Mellitus ◽

Children And Adolescents ◽

Healthy Controls ◽

25 Oh Vitamin D ◽

Bulgarian Population

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Type 1 Diabetes Mellitus Donor Mesenchymal Stromal Cells Exhibit Comparable Potency to Healthy Controls In Vitro

Stem Cells Translational Medicine ◽

10.5966/sctm.2015-0272 ◽

2016 ◽

Vol 5 (11) ◽

pp. 1485-1495 ◽

Cited By ~ 23

Author(s):

Lindsay C. Davies ◽

Jessica J. Alm ◽

Nina Heldring ◽

Guido Moll ◽

Caroline Gavin ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Type 1 Diabetes Mellitus ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Healthy Controls

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Sex-specific association of PTPN22 1858T with type 1 diabetes but not with Hashimoto’s thyroiditis or Addison’s disease in the German population

Acta Endocrinologica ◽

10.1530/eje.1.02035 ◽

2005 ◽

Vol 153 (6) ◽

pp. 895-899 ◽

Cited By ~ 74

Author(s):

Heinrich Kahles ◽

Elizabeth Ramos-Lopez ◽

Britta Lange ◽

Oliver Zwermann ◽

Martin Reincke ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Type 1 Diabetes Mellitus ◽

Hashimoto’S Thyroiditis ◽

Addison’S Disease ◽

Hashimoto's Thyroiditis ◽

Addison's Disease ◽

Healthy Controls ◽

Significant Difference

Background: Endocrine autoimmune disorders share genetic susceptibility loci, causing a disordered T-cell activation and homeostasis (HLA class II genes, CTLA-4). Recent studies showed a genetic variation within the PTPN22 gene to be an additional risk factor. Materials and Methods: Patients with type 1 diabetes (n = 220), Hashimoto’s thyroiditis (n = 94), Addison’s disease (n = 121) and healthy controls (n = 239) were genotyped for the gene polymorphism PTPN22 1858 C/T. Results: Our study confirms a significant association between allelic variation of the PTPN22 1858 C/T polymorphism and type 1 diabetes mellitus (T1D). 1858T was observed more frequently in T1D patients (19.3% vs 11.3%, P = 0.0009; odds ratio for allele T = 1.88, 95% confidence interval [1.3–2.7]). Furthermore, we found a strong association in female patients with T1D (P = 0.0003), whereas there was no significant difference between male patients with type 1 diabetes and male controls. No significant difference was observed between the distribution of PTPN22 C/T in patients with Hashimoto’s thyroiditis or Addison’s disease and healthy controls. Conclusion: The PTPN22 polymorphism 1858 C/T may be involved in the pathogenesis of type 1 diabetes mellitus by a sex-specific mechanism that contributes to susceptibility in females.

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Auditory and visual reaction time in athletes, healthy controls, and patients of type 1 diabetes mellitus: A comparative study

International Journal of Diabetes in Developing Countries ◽

10.4103/0973-3930.32170 ◽

2006 ◽

Vol 26 (3) ◽

pp. 112 ◽

Cited By ~ 8

Author(s):

Prabhjot Kaur ◽

Maman Paul ◽

JaspalSingh Sandhu

Keyword(s):

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Reaction Time ◽

Comparative Study ◽

Type 1 Diabetes Mellitus ◽

Healthy Controls ◽

Visual Reaction Time ◽

Visual Reaction

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Exhaled volatile substances in children suffering from type 1 diabetes mellitus: results from a cross-sectional study

Scientific Reports ◽

10.1038/s41598-019-52165-x ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Phillip Trefz ◽

Juliane Obermeier ◽

Ruth Lehbrink ◽

Jochen K. Schubert ◽

Wolfram Miekisch ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Type 1 Diabetes Mellitus ◽

Metabolic Control ◽

Cross Sectional Study ◽

Healthy Controls ◽

Sectional Study ◽

Cross Sectional ◽

Non Invasive

Abstract Monitoring metabolic adaptation to type 1 diabetes mellitus in children is challenging. Analysis of volatile organic compounds (VOCs) in exhaled breath is non-invasive and appears as a promising tool. However, data on breath VOC profiles in pediatric patients are limited. We conducted a cross-sectional study and applied quantitative analysis of exhaled VOCs in children suffering from type 1 diabetes mellitus (T1DM) (n = 53) and healthy controls (n = 60). Both groups were matched for sex and age. For breath gas analysis, a very sensitive direct mass spectrometric technique (PTR-TOF) was applied. The duration of disease, the mode of insulin application (continuous subcutaneous insulin infusion vs. multiple daily insulin injection) and long-term metabolic control were considered as classifiers in patients. The concentration of exhaled VOCs differed between T1DM patients and healthy children. In particular, T1DM patients exhaled significantly higher amounts of ethanol, isopropanol, dimethylsulfid, isoprene and pentanal compared to healthy controls (171, 1223, 19.6, 112 and 13.5 ppbV vs. 82.4, 784, 11.3, 49.6, and 5.30 ppbV). The most remarkable differences in concentrations were found in patients with poor metabolic control, i.e. those with a mean HbA1c above 8%. In conclusion, non-invasive breath testing may support the discovery of basic metabolic mechanisms and adaptation early in the progress of T1DM.

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