Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma

Onychomycosis is one of the most common nail disorders. Its current treatment is not satisfactorily effective and often causes adverse side effects. This study aims to determine the optimal conditions for non-thermal plasma (NTP) inactivation of the most common dermatophytes in vitro and to apply it in patient`s therapy. The in vitro exposure to NTP produced by negative DC corona discharge caused full inactivation of Trichophyton spp. if applied during the early growth phases. This effect decreased to negligible inactivation with the exposure applied six days after inoculation. In a group of 40 patients with onychomycosis, NTP therapy was combined with nail plate abrasion and refreshment (NPAR) or treatment with antimycotics. The cohort included 17 patients treated with NPAR combined with NTP, 11 patients treated with antimycotics and NTP, and 12 patients treated with NPAR alone. The combination of NPAR and NTP resulted in clinical cure in more than 70% of patients. The synergistic effect of NPAR and NTP caused 85.7% improvement of mycological cure confirmed by negative microscopy and culture of the affected nail plate. We conclude that NTP can significantly improve the treatment of onychomycosis.

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Understanding the multi-scale structure, physicochemical properties and in vitro digestibility of citrate naked barley starch induced by non-thermal plasma

Food & Function ◽

10.1039/d1fo00678a ◽

2021 ◽

Author(s):

Huishan Shen ◽

Xiangzhen Ge ◽

Bo Zhang ◽

Chunyan Su ◽

Qian Zhang ◽

...

Keyword(s):

Physicochemical Properties ◽

Thermal Plasma ◽

Scale Structure ◽

In Vitro Digestibility ◽

Multi Scale ◽

Naked Barley ◽

Starch Modification ◽

Plasma Pretreatment ◽

Non Thermal Plasma

Non-thermal plasma is an emerging and effective starch modification technology. In this paper, plasma pretreatment was used to modify the citrate naked barley starch for enhancing the accessibility of citric...

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Non-thermal plasma-treated solution demonstrates antitumor activity against pancreatic cancer cells in vitro and in vivo

Scientific Reports ◽

10.1038/s41598-017-08560-3 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 53

Author(s):

Kim Rouven Liedtke ◽

Sander Bekeschus ◽

André Kaeding ◽

Christine Hackbarth ◽

Jens-Peter Kuehn ◽

...

Keyword(s):

Pancreatic Cancer ◽

Antitumor Activity ◽

Cancer Cells ◽

Thermal Plasma ◽

Pancreatic Cancer Cells ◽

Non Thermal Plasma

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Effects of non-thermal plasma treatment on the polysaccharide from Dendrobium nobile Lindl. And its immune activities in vitro

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2019.10.260 ◽

2020 ◽

Vol 153 ◽

pp. 942-950

Author(s):

Yijun Fan ◽

Qingsong Yu ◽

Gang Wang ◽

Jingwen Tan ◽

Sha Liu ◽

...

Keyword(s):

Plasma Treatment ◽

Thermal Plasma ◽

Dendrobium Nobile ◽

Non Thermal Plasma ◽

Dendrobium Nobile Lindl

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Non-Thermal Plasma Accelerates Astrocyte Regrowth and Neurite Regeneration Following Physical Trauma In Vitro

Applied Sciences ◽

10.3390/app9183747 ◽

2019 ◽

Vol 9 (18) ◽

pp. 3747 ◽

Cited By ~ 4

Author(s):

Kritika S. Katiyar ◽

Abraham Lin ◽

Alexander Fridman ◽

Carolyn E. Keating ◽

D. Kacy Cullen ◽

...

Keyword(s):

Nervous System ◽

Thermal Plasma ◽

Cell Types ◽

Neural Regeneration ◽

Mechanical Trauma ◽

Post Injury ◽

Physical Trauma ◽

Non Thermal Plasma ◽

Differentiation And Proliferation

Non-thermal plasma (NTP), defined as a partially ionized gas, is an emerging technology with several biomedical applications, including tissue regeneration. In particular, NTP treatment has been shown to activate endogenous biological processes to promote cell regrowth, differentiation, and proliferation in multiple cell types. However, the effects of this therapy on nervous system regeneration have not yet been established. Accordingly, the current study explored the effects of a nanosecond-pulsed dielectric barrier discharge plasma on neural regeneration. Following mechanical trauma in vitro, plasma was applied either directly to (1) astrocytes alone, (2) neurons alone, or (3) neurons or astrocytes in a non-contact co-culture. Remarkably, we identified NTP treatment intensities that accelerated both neurite regeneration and astrocyte regrowth. In astrocyte cultures alone, an exposure of 20–90 mJ accelerated astrocyte re-growth up to three days post-injury, while neurons required lower treatment intensities (≤20 mJ) to achieve sub-lethal outgrowth. Following injury to neurons in non-contact co-culture with astrocytes, 20 mJ exposure of plasma to only neurons or astrocytes resulted in increased neurite regeneration at three days post-treatment compared to the untreated, but no enhancement was observed when both cell types were treated. At day seven, although regeneration further increased, NTP did not elicit a significant increase from the control. However, plasma exposure at higher intensities was found to be injurious, underscoring the need to optimize exposure levels. These results suggest that growth-promoting physiological responses may be elicited via properly calibrated NTP treatment to neurons and/or astrocytes. This could be exploited to accelerate neurite re-growth and modulate neuron-astrocyte interactions, thereby hastening nervous system regeneration.

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