scholarly journals Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study

2020 ◽  
Vol 6 (4) ◽  
pp. 375
Author(s):  
Juliana Lebeau ◽  
Thomas Petit ◽  
Mireille Fouillaud ◽  
Laurent Dufossé ◽  
Yanis Caro
Keyword(s):  
Ionic Liquids ◽  
Filamentous Fungi ◽  
Brown Pigment ◽  
Phase System ◽  
Red Pigment ◽  
Submerged Cultures ◽  
Two Phase ◽  
Red Pigments ◽  
Fermentation Broths ◽  
Fungal Pigments

Demand for microbial colorants is now becoming a competitive research topic for food, cosmetics and pharmaceutics industries. In most applications, the pigments of interest such as polyketide-based red pigments from fungal submerged cultures are extracted by conventional liquid–liquid extraction methods requiring large volumes of various organic solvents and time. To address this question from a different angle, we proposed, here, to investigate the use of three different aqueous two-phase extraction systems using either ammonium- or imidazolium-based ionic liquids. We applied these to four fermentation broths of Talaromyces albobiverticillius (deep red pigment producer), Emericella purpurea (red pigment producer), Paecilomyces marquandii (yellow pigment producer) and Trichoderma harzianum (yellow-brown pigment producer) to investigate their selective extraction abilities towards the detection of polyketide-based pigments. Our findings led us to conclude that (i) these alternative extraction systems using ionic liquids as greener extractant means worked well for this extraction of colored molecules from the fermentation broths of the filamentous fungi investigated here; (ii) tetrabutylammonium bromide, [N4444]Br-, showed the best pigment extraction ability, with a higher putative affinity for azaphilone red pigments; (iii) the back extraction and recovery of the fungal pigments from ionic liquid phases remained the limiting point of the method under our selected conditions for potential industrial applications. Nevertheless, these alternative extraction procedures appeared to be promising ways for the detection of polyketide-based colorants in the submerged cultures of filamentous fungi.

A two-phase system for the clean and high yield synthesis of furylmethane derivatives over –SO3H functionalized ionic liquids

2017 ◽  
Vol 19 (20) ◽  
pp. 4804-4810 ◽  
Author(s):  
S. H. Shinde ◽  
C. V. Rode
Keyword(s):  
Ionic Liquids ◽  
Reaction System ◽  
High Yield ◽  
Phase System ◽  
Phase Reaction ◽  
Two Phase ◽  
Yield Production ◽  
High Yield Production

A new and effective unique two-phase reaction system for the high yield production of tri(furyl)methane from furfural and furan.

scholarly journals Alternative Extraction and Characterization of Nitrogen-Containing Azaphilone Red Pigments and Ergosterol Derivatives from the Marine-Derived Fungal Talaromyces sp. 30570 Strain with Industrial Relevance

2020 ◽  
Vol 8 (12) ◽  
pp. 1920
Author(s):  
Juliana Lebeau ◽  
Thomas Petit ◽  
Mireille Fouillaud ◽  
Laurent Dufossé ◽  
Yanis Caro
Keyword(s):  
Nitrogen Sources ◽  
Pharmaceutical Products ◽  
Water Soluble ◽  
Cell Factory ◽  
Submerged Cultures ◽  
Fungal Cell ◽  
Red Pigments ◽  
Produce Water ◽  
Fungal Pigments ◽  
Industrial Relevance

Many species of Talaromyces of marine origin could be considered as non-toxigenic fungal cell factory. Some strains could produce water-soluble active biopigments in submerged cultures. These fungal pigments are of interest due to their applications in the design of new pharmaceutical products. In this study, the azaphilone red pigments and ergosterol derivatives produced by a wild type of Talaromyces sp. 30570 (CBS 206.89 B) marine-derived fungal strain with industrial relevance were described. The strain was isolated from the coral reef of the Réunion island. An alternative extraction of the fungal pigments using high pressure with eco-friendly solvents was studied. Twelve different red pigments were detected, including two pigmented ergosterol derivatives. Nine metabolites were identified using HPLC-PDA-ESI/MS as Monascus-like azaphilone pigments. In particular, derivatives of nitrogen-containing azaphilone red pigment, like PP-R, 6-[(Z)-2-Carboxyvinyl]-N-GABA-PP-V, N-threonine-monascorubramin, N-glutaryl-rubropunctamin, monascorubramin, and presumed N-threonyl-rubropunctamin (or acid form of the pigment PP-R) were the major pigmented compounds produced. Interestingly, the bioproduction of these red pigments occurred only when complex organic nitrogen sources were present in the culture medium. These findings are important for the field of the selective production of Monascus-like azaphilone red pigments for the industries.

Hydrophobic ionic liquids-assisted polymer recovery during penicillin extraction in aqueous two-phase system

2009 ◽  
Vol 147 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Yangyang Jiang ◽  
Hansong Xia ◽  
Jiang Yu ◽  
Chen Guo ◽  
Huizhou Liu
Keyword(s):  
Ionic Liquids ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Hydrophobic Ionic Liquids

scholarly journals Extraction of Fe (III) and Zn (II) by ionic liquids in a system based on polypropylene glycol 425 and sodium chloride.

2020 ◽  
Vol 11 (3-2020) ◽  
pp. 72-75
Author(s):  
I. V. Zinov’eva ◽  
◽  
M. I. Fedorova ◽  
Yu. A. Zakhodyaeva ◽  
A.A. Voshkin ◽  
...  
Keyword(s):  
Experimental Study ◽  
Ionic Liquids ◽  
Sodium Chloride ◽  
Phase System ◽  
Polypropylene Glycol ◽  
Two Phase ◽  
Study Results ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Extraction Of Metals

This work presents the experimental study results of Fe(III) and Zn(II) extraction by ionic liquids based on trioctylmethylammonium chloride and rhodanide in an aqueous two-phase system based on polypropylene glycol 425 and sodium chloride. Based on the results obtained, it was concluded that these extractants are effective for the extraction of metals from aqueous solutions, and aqueous two-phase systems represent a “green” alternative to traditional extraction systems.

Partitioning Behavior of Penicillin G in Aqueous Two Phase System Formed by Ionic Liquids and Phosphate

2006 ◽  
Vol 41 (12) ◽  
pp. 2849-2858 ◽  
Author(s):  
Qingfen Liu ◽  
Jiang Yu ◽  
Wangliang Li ◽  
Xuesheng Hu ◽  
Hansong Xia ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Penicillin G ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Partitioning Behavior

Partitioning Behavior of Lysozyme and α-lactalbumin in Aqueous Two-Phase System Formed by Ionic Liquids and Potassium Phosphate

2017 ◽  
Vol 13 (10) ◽  
Author(s):  
Vanessa S. Sampaio ◽  
Renata C. F. Bonomo ◽  
Cristiane M. Veloso ◽  
Rita C. S. Sousa ◽  
Evaldo C. S. Júnior ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Partition Coefficients ◽  
Potassium Phosphate ◽  
Inorganic Salts ◽  
Phase System ◽  
Two Phase ◽  
Separation Processes ◽  
Different Temperatures ◽  
Aqueous Two Phase Systems ◽  
Increasing Temperature

AbstractNowadays ionic liquids (ILs), because of their “green” characteristics, have been used for analytical and separation processes. Therefore the partitioning of lysozyme and α-lactalbumin using aqueous two-phase systems (ATPSs) composed of an ionic liquid (chloride 1-ethyl-3-methylimidazolium) and inorganic salts (K2HPO4, KH2PO4) was studied. Phase equilibrium diagrams were obtained to explore the effect of the different temperatures (293.15, 303.15, 313.15, and 323.15) K and pH (7.5, 8.0, and 8.5) used for the partitioning studies. For both proteins, partition coefficients decreased with increasing temperature. The pH influenced the partition coefficients of lysozyme and α-lactalbumin. The thermodynamic parameters (ΔH◦, ΔS◦, ΔG◦) indicate thermodynamic differences between the partitioning of lysozyme and α-lactalbumin in this system. The ΔH◦, ΔS◦, and ΔG◦values of the process studied showed that this process is spontaneous. This work demonstrates the possible use of ATPSs with ILs and inorganic salts as a methodology for the partitioning of lysozyme and α-lactalbumin.

Partitioning Behavior of Penicillin G in Aqueous Two Phase System Based on Ionic Liquids

2013 ◽  
Vol 864-867 ◽  
pp. 324-327
Author(s):  
Bin Jiang ◽  
Zhi Biao Feng ◽  
Chun Hong Liu ◽  
Ying Cao Xu ◽  
Dong Mei Li ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Extraction Yield ◽  
Penicillin G ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Partitioning Behavior

An aqueous two-phase system (ATPS) was presented with hydrophilic ionic liquid 1-Butyl-3-methylimid-azolium tetrafluoroborate ([BmiBF4) and salts for the extraction of penicillin G in this paper. The phase forming abilities of different salts were investigated to choose a suitable salt.The partitioning behavior of penicillin G in the ATPS was investigated.Concentrations of (NH4)2SO4, penicillin G, and [BmiBF4 were evaluated to determine their effects on the extraction yield of penicillin G. It was found that extraction yield strongly depended on the concentration of [BmiBF4, penicillin and (NH4)2SO4. A high extraction yield around 93% was achieved with the following parameters: (NH4)2SO4 20% (wt%), penicillin 40000 ~ 45000 U/mL, [BmiBF4 20% (wt%).

Sustainable vanadium(V)-catalyzed oxybromination of styrene: Two-phase system versus ionic liquids

2005 ◽  
Vol 77 (9) ◽  
pp. 1575-1581 ◽  
Author(s):  
Valeria Conte ◽  
Barbara Floris ◽  
Pierluca Galloni ◽  
Adriano Silvagni
Keyword(s):  
Ionic Liquids ◽  
Aqueous Phase ◽  
Chlorinated Solvents ◽  
Reaction Rates ◽  
Point Of View ◽  
Metal Catalyst ◽  
Phase System ◽  
Two Phase ◽  
System Versus ◽  
Bromide Ion

Oxybromination reaction of styrene was performed in a two-phase system of water/ionic liquids (ILs). The aim of the work was to make the mild and efficient two-phase system previously developed for the vanadium(V)-catalyzed oxybromination of alkenes, inspired by the activity of haloperoxidase enzymes, even more interesting from a sustainable point of view. As in that case, a brominating intermediate was formed from the metal catalyst, H2O2, and bromide ion in the acid aqueous phase, but chlorinated solvents were replaced with ILs.[bmim+][PF6-], [bm2im+][PF6-], [bmim+][BF4-], [bmim+][CF3SO3-], and [bmim+][(CF3SO2)2N-] were tested. We report on interesting results in terms of reaction rates and selectivities.

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