scholarly journals Effects of Different Fertilizer Treatments on Rhizosphere Soil Microbiome Composition and Functions

Land ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 329
Author(s):  
Yanan Li ◽  
Chengyu Wang ◽  
Tianye Wang ◽  
Yutao Liu ◽  
Shuxia Jia ◽  
...  

Fertilization influences the soil microbiome. However, little is known about the effects of long-term fertilization on soil microbial metabolic pathways. In this study, we investigated the soil microbiome composition and function and microbial participation in the N cycle according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) functional annotation of different genes in a metagenomic analysis after long-term fertilization. Fertilizer application significantly changed the soil C/N ratio. Chemical fertilizer (NPK) treatment decreased soil pH, and chemical fertilizer combined with straw (NPK+S0.5) treatment increased ammonium nitrogen (NH4+-N) but decreased nitrate nitrogen (NO3−-N). NPK, NPK+S0.5 and S0.5 applications did not change the soil microbiome composition or dominant phylum but changed the relative abundances of microbiome components. Moreover, fertilizer significantly influenced metabolic processes, cellular processes and single-organism processes. Compared with a no-fertilizer treatment (CK), the NPK treatment resulted in more differentially expressed gene (DEG) pathways than the NPK+S0.5 and S0.5 treatments, and these pathways significantly correlated with soil nitrate nitrogen (NO3−-N), available phosphorus (AP) and the moisture content of soil (MC). KEGG analysis found that fertilizer application mainly affected the ribosome, photosynthesis and oxidative phosphorylation pathways. S0.5 and NPK+S0.5 increased microbial nitrogen fixation, and NPK and NPK+S0.5 decreased amoA and amoB and accelerated denitrification. Thus, organic fertilizer increased N fixation and nitrification, and inorganic N fertilizer accelerated denitrification. We found that the function of the soil microbiome under different fertilizer applications could be important for the rational application of fertilizer and for environmental and sustainable development.

2021 ◽  
Vol 74 (3) ◽  
pp. 9643-9653
Author(s):  
Ratih Sandrakirana ◽  
Zainal Arifin

Soybean is known for its high protein content, which is the reason why it is widely used as one of the main food sources for humans and animals. In order to optimize soybean growth, farmers tend to add excessive dosage of chemical fertilizer to this crop. Furthermore, a continuous chemical fertilizer application without organic fertilizer addition may cause a rapid depletion of nutrients in the soil. This study aimed to evaluate the effectiveness of organic fertilizer treatment to reduce the amount of urea as chemical fertilizer needed in soybean cultivation. A complete randomized design was conducted using 21 treatments of organic and chemical fertilizer in triplicate with a 4x3 m plot size. Analysis of variance was carried out to compare the means of measurement data and Duncan multiple range test (DMRT 5%) was applied. The treatment 2,000 kg ha-1 compost + 50 kg ha-1 urea (O2K2A1) resulted the highest dry yield in soybean and had significant differences with urea-only treatment. A mixture of chemical and organic fertilizers had no significant result over the yield compared to the use of chemical fertilizer only. Compost application of 1,000-2,000 kg ha-1 with urea 50-100 kg ha-1 (O2K2A1 and O 2K1A2) showed an increase in seed yield of 35-38 % with a profit reaching 333-340 USD ha-1 compared to standard treatment using urea 50 kg ha -1 + SP-36 50 kg ha-1 + 50 KCl kg ha-1 (O0K0A1).


2010 ◽  
Vol 113-116 ◽  
pp. 1332-1335 ◽  
Author(s):  
Ning Liu ◽  
Hong Bo He ◽  
Hong Tu Xie ◽  
Zhen Bai ◽  
Xu Dong Zhang

Fertilization is one of the essential managements to maintain and increase soil organic carbon (SOC) level in agroecosystems. It has been realized that fertilizer applications influenced the turnover of labile and refractory organic carbon pools in arable soil markedly. However, the dynamic of relatively refractory lignin in response to fertilization is still kept unclear. Therefore, the impact of long-term organic fertilization on the content and degradation degree of lignin in Mollisol was investigated. Lignin monomers were released by alkaline CuO oxidation method and quantified by gas chromatography (GC). At the time scale of decades, lignin was clearly accumulated in soil and the relative accumulation of lignin in SOC was evident after long-term organic fertilizer application. Compared with the unfertilized soil, lower acid to aldehyde ratios of vanillyl and syringyl units induced by organic fertilization suggested a lower degradation degree of lignin incorporated into soil to some extent. It could be concluded that long-term organic fertilization was an effective fertilizer practice for lignin accumulation in soil and SOC sequestration in Mollisol in northeast of China.


2019 ◽  
Vol 11 (4) ◽  
pp. 1165 ◽  
Author(s):  
Haixia Wu ◽  
Yan Ge

This paper takes 516 households who planted wheat in Heyang County, Shaanxi Province in 2018, as samples to construct three policy environments: Technological guidance for planting, subsidies for organic fertilizer application, and agricultural tailwater discharge standards. The experimental choice method was used to empirically analyze policy preferences during the process of fertilizer reduction. The results indicate that households show different preferences for the three policy settings: The fertilizer application rate is reduced by 6.98% if providing full technological guidance for farmers throughout the wheat planting process and is reduced by 5.18% under the background of providing appropriate organic fertilizer subsidies. The agricultural tailwater discharge standards have the least impact on the reducing level of chemical fertilizer application, with decreasing amounts of only 1.85% and 0.77% under the second-level and the first-level agricultural tailwater discharge standards, respectively. These results indicate that households in Heyang County, Shaanxi Province, demonstrate a low willingness to accept the agricultural tailwater discharge standards in order to cut down on the amount of chemical fertilizer application and the agricultural non-point source pollution. Therefore, compared with a compounded annual growth rate (CAGR) of 1% of fertilizer usage nationwide according to the Chinese Ministry of Agriculture, given the current planting environment and policies design, providing comprehensive technological guidance as well as price subsidies for the organic fertilizer can significantly and robustly reduce the excessive application of fertilizer in Heyang County, Shaanxi Province, under the best scenario, thereby further alleviating agricultural non-point source pollution.


HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 464D-464 ◽  
Author(s):  
Mark Gaskell

Organic vegetable production acreage is expanding in California, but little research-based information is available to guide growers. Several new organic fertilizer materials are available but little data exists on efficient use of these materials. During 1998, the following materials: compost (C), pelleted chicken manure (PCM), fish meal (FM), liquid fish (LF), liquid soybean meal (LSM), feather meal (FTM), and seabird guano (SG) were evaluated. Each material was applied at treatment rates of 0, 60, 120, and 180 kg nitrogen (N)/ha to transplanted, sprinkler irrigated bell peppers. The materials were applied as 30N pre-transplant (PRE) and 30N at 20 days post-transplant (POST) for the 60N treatment; 60N PRE and 30N at 20 days POST and 30N at 40 days POST for the 120N treatment; and 60N PRE, 30N at 20 days POST, 45N at 40 days POST, and 45N at 70 days POST for the 180N treatment. Weekly soil nitrate nitrogen (SSN) over 16 weeks POST and fresh pepper yield was determined for all treatments. Weekly SSN varied from lows of 4 mg·kg-1 in 0N-treated plots to over 80 mg·kg-1 in FTM 180N-treated plots. Highest SSN was observed in FTM-, SG-, LSM-, LF-, and FM-treated plots at 180N and peaks in SSN lagged fertilizer application 3 to 4 weeks. Total pepper yield was not as markedly affected as early yield and size. Highest early yield and largest sizes were observed in FTM 180N-treated plots. Compost treated plots at 180N produced highest economic return per fertilizer dollar.


PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256754
Author(s):  
Haiming Tang ◽  
Chao Li ◽  
Lihong Shi ◽  
Xiaoping Xiao ◽  
Kaikai Cheng ◽  
...  

Soil microorganism plays an important role in nitrogen (N) fixation process of paddy field, but the related information about how soil microorganism that drive N fixation process response to change of soil phy-chemical characteristics under the double-cropping rice (Oryza sativa L.) paddy field in southern of China is need to further study. Therefore, the impacts of 34-years different long-term fertilization system on soil N-fixing bacteria community under the double-cropping rice paddy field in southern of China were investigated by taken chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method in this paper. The field experiment were set up four different fertilizer treatments: chemical fertilizer alone (MF), rice straw and chemical fertilizer (RF), 30% organic manure and 70% chemical fertilizer (OM), and unfertilized as a control (CK). This results showed that compared with CK treatment, the diversity index of cbbLR and nifH genes with OM and RF treatments were significantly increased (p<0.05), respectively. Meanwhile, the abundance of cbbLR gene with OM, RF and MF treatments were increased by 23.94, 12.19 and 6.70×107 copies g-1 compared to CK treatment, respectively. Compared with CK treatment, the abundance of nifH gene with OM, RF and MF treatments were increased by 23.90, 8.82 and 5.40×109 copies g-1, respectively. This results indicated that compared with CK treatment, the soil autotrophic azotobacter and nitrogenase activities with OM and RF treatments were also significantly increased (p<0.05), respectively. There were an obvious difference in features of soil N-fixing bacteria community between application of inorganic fertilizer and organic manure treatments. Therefore, this results demonstrated that abundance of soil N-fixing bacteria community in the double-cropping rice paddy field were increased by long-term applied with organic manure and crop residue managements.


2021 ◽  
Vol 23 (1) ◽  
pp. 46-52
Author(s):  
Erin Puspita Rini ◽  
Sugiyanta Sugiyanta

[CABBAGE (Brassica olacea var. capitata) GROWTH AND YIELD AFFECTED BY COMBINATION OF ORGANIC AND INORGANIC FERTILIZER APPLICATIONS]. The use of organic fertilizer increasingly sought by farmers due to the increase in demand for organic products by consumers and also awareness of the sustainability of the land. The use of organic fertilizers in the cultivation alone could improve the soil quality but needs to be balanced with inorganic fertilizers to meet the nutrient adequacy. This study aims to examine the effect of organic and inorganic fertilizers combination on the growth and cabbage yield, and also to determine the most efficient dosage combination of organic and inorganic fertilizers. This research was conducted at the IPB Pasir Sarongge Experimental Station, Cianjur, West Java from November 2020 to February 2021. The study was compiled using RCBD with 4 replications and 7 treatments. The results showed the combination of 0.75 doses of inorganic fertilizer (150 kg/ha of urea, 75 kg/ha SP36, and 75 kg/ha KCl) and 3 tons/ha of organic fertilizer could increase 14.87 to 15.44% plant height and the number of leaves at 12.82 -15.11% compared to the same dose of inorganic fertilizer treatment alone. The combination of 1 dose (200 kg/ha of urea, 100 kg/ha SP36, and 100 kg/ha KCl) inorganic fertilizer application and 2 tonnes/ha of organic fertilizer could increase 50,60% yield/plot and yield/ha cabbage than 1 dose of inorganic fertilizer treatment..


2019 ◽  
Vol 11 (8) ◽  
pp. 2424
Author(s):  
Jianli Liao ◽  
Jun Ye ◽  
Yun Liang ◽  
Muhammad Khalid ◽  
Danfeng Huang

A high level of antioxidants in organic-produced vegetables has been attributed to soil conditions; however, little is known about the relationships between antioxidants and rhizobacteria under different fertilization treatments. A pot trial for pakchoi (Brassica campestris ssp. chinensis L.) was conducted under greenhouse conditions with: (1) control; (2) chemical fertilizer; and (3) organic fertilizer. The responses of the plant, soil properties, and rhizobacterial community were measured after 45 days of cultivation. Fertilization increased soil nutrient levels and pakchoi productivity and the reshaped rhizobacterial community structure, while no differences in rhizobacterial abundance and total diversity were observed. Generally, most plant antioxidants were negatively correlated with inorganic nitrogen (N) and positively correlated to organic N in soil. The genera of Arthrospira and Acutodesmus contained differential rhizobacteria under chemical fertilizer treatment, which are known as copiotrophs. In addition, the addition of a chemical fertilizer may stimulate organic substance turnover by the enrichment of organic compound degraders (e.g., Microbacterium and Chitinophaga) and the promotion of predicted functional pathways involved in energy metabolism. Several beneficial rhizobacteria were associated with organic fertilizer amended rhizosphere including the genera Bacillus, Mycobacterium, Actinomycetospora, and Frankia. Furthermore, Bacillus spp. were positively correlated with plant biomass and phenolic acid. Moreover, predictive functional profiles of the rhizobacterial community involved in amino acid metabolism and lipid metabolism were significantly increased under organic fertilization, which were positively correlated with plant antioxidant activity. Overall, our study suggests that the short-term application of chemical and organic fertilizers reshapes the rhizobacterial community structure, and such changes might contribute to the plant’s performance.


Author(s):  
P. H. Kopytko ◽  
◽  
R. V. Yakovenko

The issue of scientifically sound fertilizer application in fruit plantations, which are long-term and re-grown in one place remains insufficiently studied. To solve this problem is possible only in long-term stationary studies, as the impact of different fertilizer systems on changes in soil properties and tree productivity for a long period of their use. The results of researches of long-term fertilizer influence on the main fertility indices of dark gray podzolic heavy loam soil and productivity of repeatedly grown apple trees of Idared varieties on seed and vegetative (M4) rootstocks and Calville snow on seedling rootstock are considered. During the 85-year period of growing the first and second generation of apple trees in the experimental garden, the organic fertilizer (40 t/ha of cattle manure), mineral fertilizer (N120P120K120) and their combination (20 t/ha of manure + N60P60K60) were applied in the old plantation every two years in autumn in plowing in rows at 18–20 cm, and in the new repetition: manure, phosphorus and potassium fertilizers as well, and nitrogen fertilizer in half doses annually in spring for cultivation or disk plowing to a depth of 12–15 cm. As a result of research it was found that organic fertilizer better than mineral fertilizers provided the formation of soil fertility (humus and mobile compounds and forms of nutrients, soil reaction) and yield capacity of experimental apple trees, which for all years of fruiting exceeded the total yield of Calville snow and Idared on seedling and vegetative rootstocks, respectively, by 34.8, 27.7 and 23.4 % compared with the yield of the control non-fertilized areas and 16.0, 15.8 and 13.2 % – on those fertilized with N120Р120К120. Similar parameters of soil fertility indicators are formed by the organo-mineral fertilizer system with systematic long-term application of half the norms of organic and mineral fertilizers of manure 20 t/ha together with N60P60K60. However, the mineral system (N120P120K120) significantly less increases the humus content and content of macronutrients available for plant nutrition and does not enrich the soil with trace elements, acidifies the reaction of the soil environment


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