Efficient Isolation of Bacterial RNAs Using Silica-Based Materials Modified with Ionic Liquids

High quality nucleic acids (with high integrity, purity, and biological activity) have become indispensable products of modern society, both in molecular diagnosis and to be used as biopharmaceuticals. As the current methods available for the extraction and purification of nucleic acids are laborious, time-consuming, and usually rely on the use of hazardous chemicals, there is an unmet need towards the development of more sustainable and cost-effective technologies for nucleic acids purification. Accordingly, this study addresses the preparation and evaluation of silica-based materials chemically modified with chloride-based ionic liquids (supported ionic liquids, SILs) as potential materials to effectively isolate RNAs. The investigated chloride-based SILs comprise the following cations: 1-methyl-3-propylimidazolium, triethylpropylammonium, dimethylbutylpropylammonium, and trioctylpropylammonium. All SILs were synthesized by us and characterized by solid-state 13C Nuclear Magnetic Resonance (NMR), Scanning Electron Microscopy (SEM), elemental analysis, and zeta potential measurements, confirming the successful covalent attachment of each IL cation with no relevant changes in the morphology of materials. Their innovative application as chromatographic supports for the isolation of recombinant RNA was then evaluated. Adsorption kinetics of transfer RNA (tRNA) on the modified silica-based materials were investigated at 25 °C. Irrespective to the immobilized IL, the adsorption experimental data are better described by a pseudo first-order model, and maximum tRNA binding capacities of circa 16 µmol of tRNA/g of material were achieved with silica modified with 1-methyl-3-propylimidazolium chloride and dimethylbutylpropylammonium chloride. Furthermore, the multimodal character displayed by SILs was explored towards the purification of tRNA from Escherichia coli lysates, which in addition to tRNA contain ribosomal RNA and genomic DNA. The best performance on the tRNA isolation was achieved with SILs comprising 1-methyl-3-propylimidazolium chloride and dimethylbutylpropylammonium chloride. Overall, the IL modified silica-based materials represent a more efficient, sustainable, and cost-effective technology for the purification of bacterial RNAs, paving the way for their use in the purification of distinct biomolecules or nucleic acids from other sources.

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The WHO World Mental Health (WMH) Surveys

Die Psychiatrie ◽

10.1055/s-0038-1671923 ◽

2009 ◽

Vol 06 (01) ◽

pp. 5-9 ◽

Cited By ~ 10

Author(s):

S. Aguilar-Gaxiola ◽

J. Alonso ◽

S. Chatterji ◽

S. Lee ◽

T. B. Üstün ◽

...

Keyword(s):

Mental Health ◽

Mental Disorders ◽

Treatment Effectiveness ◽

Age Of Onset ◽

Common Mental Disorders ◽

Mental Health Policy ◽

Unmet Need ◽

Cost Effective ◽

World Mental Health ◽

Prevalence Estimates

SummaryThe paper presents an overview of the WHO World Mental Health (WMH) Survey Initiative and summarizes recent WMH results regarding the prevalence and societal costs of mental disorders. The WMH surveys are representative community surveys that were carried out in 28 countries throughout the world aimed at providing information to mental health policy makers about the prevalence, burden, and unmet need for treatment of common mental disorders. Results show that mental disorders are commonly occurring in all participating countries. The inter-quartile range (IQR: 25th-75th percentiles) of lifetime DSM-IV disorder prevalence estimates (combining anxiety, mood, disruptive behavior, and substance disorders) is 18.1-36.1%. The IQR of 12-month prevalence estimates is 9.8-19.1%. Analysis of age-of-onset reports shows that many mental disorders begin in childhood-adolescence and have significant adverse effects on subsequent role transitions. Adult mental disorders are found in the WMH data to be associated with high levels of role impairment. Despite this burden, the majority of mental disorders go untreated. Although these results suggest that expansion of treatment could be cost-effective from both the employer perspective and the societal perspective, treatment effectiveness trials are needed to confirm this suspicion. The WMH results regarding impairments are being used to target several such interventions.

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Radiation synthesis of imidazolium-based ionic liquids modified silica adsorbents for ReO4- adsorption

New Journal of Chemistry ◽

10.1039/d1nj00332a ◽

2021 ◽

Author(s):

Kangjun Xie ◽

Zhen Dong ◽

Nan Wang ◽

Wei Qi ◽

Long Zhao

Keyword(s):

Ionic Liquids ◽

Environmental Security ◽

Radioactive Wastes ◽

Modified Silica ◽

Radiation Synthesis ◽

Silica Adsorbents ◽

Selective Elimination

The selective elimination of radioactive TcO4- from radioactive wastes is becoming increasingly critical for environmental security. In this work, a series of novel adsorbents (Si-IL-X, X= NO3, Cl, BF4, NTf2)...

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Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer’s disease

Scientific Reports ◽

10.1038/s41598-019-51837-y ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 6

Author(s):

Aidan Kenny ◽

Eva M. Jiménez-Mateos ◽

María Ascensión Zea-Sevilla ◽

Alberto Rábano ◽

Pablo Gili-Manzanaro ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Unmet Need ◽

Cost Effective ◽

Specific Protein ◽

Tear Fluid ◽

Initiation Factor ◽

Non Invasive ◽

A Genome ◽

Potential Biomarker

Abstract Alzheimer’s disease (AD) is characterized by a progressive loss of neurons and cognitive functions. Therefore, early diagnosis of AD is critical. The development of practical and non-invasive diagnostic tests for AD remains, however, an unmet need. In the present proof-of-concept study we investigated tear fluid as a novel source of disease-specific protein and microRNA-based biomarkers for AD development using samples from patients with mild cognitive impairment (MCI) and AD. Tear protein content was evaluated via liquid chromatography-mass spectrometry and microRNA content was profiled using a genome-wide high-throughput PCR-based platform. These complementary approaches identified enrichment of specific proteins and microRNAs in tear fluid of AD patients. In particular, we identified elongation initiation factor 4E (eIF4E) as a unique protein present only in AD samples. Total microRNA abundance was found to be higher in tears from AD patients. Among individual microRNAs, microRNA-200b-5p was identified as a potential biomarker for AD with elevated levels present in AD tear fluid samples compared to controls. Our study suggests that tears may be a useful novel source of biomarkers for AD and that the identification and verification of biomarkers within tears may allow for the development of a non-invasive and cost-effective diagnostic test for AD.

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Method for automated extraction and purification of nucleic acids and its implementation in microfluidic system

Applied Biochemistry and Microbiology ◽

10.1134/s0003683811020128 ◽

2011 ◽

Vol 47 (2) ◽

pp. 211-220 ◽

Cited By ~ 3

Author(s):

D. D. Mamaev ◽

D. A. Khodakov ◽

E. I. Dementieva ◽

I. V. Filatov ◽

D. A. Yurasov ◽

...

Keyword(s):

Nucleic Acids ◽

Microfluidic System ◽

Automated Extraction ◽

Extraction And Purification

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Supported Ionic Liquids. New Recyclable Materials for theL-Proline-Catalyzed Aldol Reaction

Advanced Synthesis & Catalysis ◽

10.1002/adsc.200505227 ◽

2006 ◽

Vol 348 (1-2) ◽

pp. 82-92 ◽

Cited By ~ 118

Author(s):

Michelangelo Gruttadauria ◽

Serena Riela ◽

Carmela Aprile ◽

Paolo Lo Meo ◽

Francesca D'Anna ◽

...

Keyword(s):

Ionic Liquids ◽

Aldol Reaction ◽

Supported Ionic Liquids

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Supported Ionic Liquids on Solid Materials as Catalysts for the Synthesis of Propylene Glycol Methyl Ether

Asian Journal of Chemistry ◽

10.14233/ajchem.2013.13741 ◽

2013 ◽

Vol 25 (5) ◽

pp. 2722-2728 ◽

Cited By ~ 2

Author(s):

Minh Ngoc Haa ◽

Roger Whiting ◽

Sheng Han ◽

Yuhong Wang

Keyword(s):

Ionic Liquids ◽

Propylene Glycol ◽

Methyl Ether ◽

Solid Materials ◽

Supported Ionic Liquids ◽

Glycol Methyl Ether

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Effect of anion type of imidazolium based polymer supported ionic liquids on the solvent free synthesis of cycloaddition of CO2 into epoxide

Catalysis Today ◽

10.1016/j.cattod.2015.09.048 ◽

2016 ◽

Vol 265 ◽

pp. 56-67 ◽

Cited By ~ 54

Author(s):

Arvind H. Jadhav ◽

Gaurav M. Thorat ◽

Kyuyoung Lee ◽

Alan Christian Lim ◽

Hyo Kang ◽

...

Keyword(s):

Ionic Liquids ◽

Solvent Free ◽

Polymer Supported ◽

Supported Ionic Liquids ◽

Solvent Free Synthesis ◽

Anion Type

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Alternative Extraction and Downstream Purification Processes for Anthocyanins

Molecules ◽

10.3390/molecules27020368 ◽

2022 ◽

Vol 27 (2) ◽

pp. 368

Author(s):

Ana N. Nunes ◽

Alexandra Borges ◽

Ana A. Matias ◽

Maria Rosário Bronze ◽

Joana Oliveira

Keyword(s):

Cost Effective ◽

Deep Eutectic Solvents ◽

Extraction Methods ◽

Chemical Diversity ◽

Preparative Scale ◽

Extraction And Purification ◽

Cosmetic Industry ◽

Cost Effective Alternative ◽

Compressed Fluid ◽

Counter Current

Anthocyanins are natural pigments displaying different attractive colors ranging from red, violet, to blue. These pigments present health benefits that increased their use in food, nutraceuticals, and the cosmetic industry. However, anthocyanins are mainly extracted through conventional methods that are time-consuming and involve the use of organic solvents. Moreover, the chemical diversity of the obtained complex extracts make the downstream purification step challenging. Therefore, the growing demand of these high-value pigments has stimulated the interest in designing new, safe, cost-effective, and tunable strategies for their extraction and purification. The current review focuses on the potential application of compressed fluid-based (such as subcritical and supercritical fluid extraction and pressurized liquid extraction) and deep eutectic solvents-based extraction methods for the recovery of anthocyanins. In addition, an updated review of the application of counter-current chromatography for anthocyanins purification is provided as a faster and cost-effective alternative to preparative-scale HPLC.

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Novel and Reusable Mesoporous Silica Supported 4-Methylbenzenesul- fonate-functionalized Ionic Liquids for Room Temperature Highly Efficient Preparation of 2,4,5-Triaryl-1H-imidazoles

Journal of the Mexican Chemical Society ◽

10.29356/jmcs.v65i4.1529 ◽

2021 ◽

Vol 65 (4) ◽

Author(s):

Yang Liu ◽

Qi Rong ◽

Chen Chen ◽

Hu Yu Lin

Keyword(s):

Ionic Liquids ◽

Room Temperature ◽

Catalytic Mechanism ◽

Catalyst Stability ◽

Catalyst Amount ◽

Supported Ionic Liquid ◽

Highly Efficient ◽

Excellent Activity ◽

Commercial Applications ◽

Supported Ionic Liquids

Abstract. A series of mesoporous materials supported ionic liquids were prepared and tested as effective and practical catalysts for the synthesis of 2,4,5-triaryl-1H-imidazoles. The effects of type of catalysts, catalyst amount, and catalyst stability have also been investigated in detail, the catalyst Ti-SBA-15@ILTsO exhibited excellent activity in excellent yields of 92 % ~ 99 % with low catalyst amount at room temperature within 1 h. In addition, the supported ionic liquid can be easily recovered and reused for six times with satisfactory catalytic activity. Furthermore, a general synergetic catalytic mechanism for the reaction was proposed. Maybe this work employing Ti-SBA-15@ILTsO as highly efficient and stable catalyst for the synthesis of 2,4,5-triaryl-1H-imidazoles has potential commercial applications. Resumen. Se prepararon y probaron una serie de materiales mesoporosos soportados con líquidos iónicos como catalizadores eficaces y prácticos para la síntesis de 2,4,5-triaryl-1H-imidazoles. También se investigaron en detalle los efectos del tipo de catalizadores, la cantidad de catalizador y la estabilidad del catalizador. El catalizador Ti-SBA-15@ILTsO mostró una excelente actividad con rendimientos excelentes del 92 % ~ 99% con una baja cantidad de catalizador a temperatura ambiente en 1 h. Además, el líquido iónico soportado puede recuperarse fácilmente y reutilizarse durante seis veces con una actividad catalítica satisfactoria. Por otro lado, se propuso un mecanismo catalítico sinérgico general para la reacción. Este trabajo que emplea Ti-SBA-15@ILTsO como catalizador altamente eficiente y estable para la síntesis de 2,4,5-triaril-1H-imidazoles puede tener aplicaciones potencialmente comerciales.

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