scholarly journals Surface Modification of Pure Magnesium Mesh for Guided Bone Regeneration: In Vivo Evaluation of Rat Calvarial Defect

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2684 ◽  
Author(s):  
Shuang Wu ◽  
Yong-Seok Jang ◽  
Yu-Kyoung Kim ◽  
Seo-Young Kim ◽  
Seung-O Ko ◽  
...  
Keyword(s):  
Surface Modification ◽  
Bone Regeneration ◽  
Protective Layer ◽  
Guided Bone Regeneration ◽  
Bone Volume ◽  
Pure Magnesium ◽  
Calvarial Defect ◽  
Barrier Membrane ◽  
Rat Calvarial Defect

Guided bone regeneration is a therapeutic method that uses a barrier membrane to provide space available for new bone formation at sites with insufficient bone volume. Magnesium with excellent biocompatibility and mechanical properties has been considered as a promising biodegradable material for guided bone regeneration; however, the rapid degradation rate in the physiological environment is a problem to be solved. In this study, surface modification of pure magnesium mesh was conducted by plasma electrolytic oxidation and hydrothermal treatment to form a densely protective layer on the Mg substrate. The protective layer mainly consisted of Mg(OH)2 with the amorphous calcium phosphate. Then, weight loss measurement and Micro-CT imaging were performed after an immersion test in a simulated body fluid. The effect of surface modification of the magnesium mesh on the guided bone regeneration was evaluated through an in vivo test using the rat calvarial defect model. The biodegradation of the magnesium mesh was identified to be significantly retarded. Additionally, the surface modification of Mg also can improve the bone volume and bone density of calvarial defect in comparison with that of the pristine Mg mesh.

scholarly journals Properties of a bovine collagen type I membrane for guided bone regeneration applications

e-Polymers ◽  
2021 ◽  
Vol 21 (1) ◽  
pp. 210-221
Author(s):  
Igor S. Brum ◽  
Carlos N. Elias ◽  
Jorge J. de Carvalho ◽  
Jorge L. S. Pires ◽  
Mario J. S. Pereira ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Collagen Type ◽  
Mechanical Stability ◽  
Collagen Type I ◽  
Guided Bone Regeneration ◽  
Bone Volume ◽  
Male Rats ◽  
Toxicity Tests ◽  
Type I

Abstract Dental implant treatment requires an available bone volume in the implantation site to ensure the implant’s mechanical stability. When the bone volume is insufficient, one must resort to surgical means such as guided bone regeneration (GBR). In GBR surgery, bone grafts and membranes are used. The objective of this work is to manufacture and characterize the in vitro and in vivo properties of resorbable collagen type I membranes (Green Membrane®) for GBR. Membrane surface morphology was characterized by SEM and roughness was measured using an interferometric noncontact 3D system. In vivo skin sensitization and toxicity tests have been performed on Wistar rats. Bone defects were prepared in 24 adult male rats, filled with biomaterials (Blue Bone® and Bio Oss®) and covered with collagen membranes to maintain the mechanical stability of the site for bone regeneration. The incisions were closed with simple stitches; and 60 days after the surgery, the animals were euthanized. Results showed that the analyzed membrane was homogeneous, with collagen fiber webs and open pores. It had no sign of cytotoxicity and the cells at the insertion site showed no bone morphological changes. There was no tissue reaction and no statistical difference between Blue Bone® and Bio Oss® groups. The proposed membrane has no cytotoxicity and displays a biocompatibility profile that makes it suitable for GBR.

Melatonin decorated 3D-printed beta-tricalcium phosphate scaffolds promoting bone regeneration in a rat calvarial defect model

2019 ◽  
Vol 7 (20) ◽  
pp. 3250-3259 ◽  
Author(s):  
Yali Miao ◽  
Yunhua Chen ◽  
Xiao Liu ◽  
Jingjing Diao ◽  
Naru Zhao ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Calvarial Defect ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
3D Printed ◽  
Rat Calvarial Defect

3D-printed β-TCP scaffolds decorated with melatonin via dopamine mussel-inspired chemistry enhance the osteogenesis and in vivo bone regeneration.

Porous 3D Printed Scaffolds For Guided Bone Regeneration In a Rat Calvarial Defect Model

2020 ◽  
Vol 20 ◽  
pp. 100706 ◽  
Author(s):  
Hoang Phuc Dang ◽  
Cedryck Vaquette ◽  
Tara Shabab ◽  
Román A. Pérez ◽  
Ying Yang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Calvarial Defect ◽  
Defect Model ◽  
3D Printed ◽  
Rat Calvarial Defect

In vivo bone regeneration evaluation of duck’s feet collagen/PLGA scaffolds in rat calvarial defect

2017 ◽  
Vol 25 (10) ◽  
pp. 994-999 ◽  
Author(s):  
Jeong Eun Song ◽  
Nirmalya Tripathy ◽  
Jae Hun Shin ◽  
Dae Hoon Lee ◽  
Jae Geun Cha ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Calvarial Defect ◽  
Rat Calvarial Defect

scholarly journals In Vivo Analysis of the Biocompatibility and Macrophage Response of a Non-Resorbable PTFE Membrane for Guided Bone Regeneration

2018 ◽  
Vol 19 (10) ◽  
pp. 2952 ◽  
Author(s):  
Tadas Korzinskas ◽  
Ole Jung ◽  
Ralf Smeets ◽  
Sanja Stojanovic ◽  
Stevo Najman ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Foreign Body Response ◽  
Tissue Response ◽  
Collagen Membrane ◽  
Macrophage Response ◽  
Barrier Membrane ◽  
In Vivo Analysis ◽  
Inflammatory Macrophages

The use of non-resorbable polytetrafluoroethylene (PTFE) membranes is indicated for the treatment of large, non-self-containing bone defects, or multi-walled defects in the case of vertical augmentations. However, less is known about the molecular basis of the foreign body response to PTFE membranes. In the present study, the inflammatory tissue responses to a novel high-density PTFE (dPTFE) barrier membrane have preclinically been evaluated using the subcutaneous implantation model in BALB/c mice by means of histopathological and histomorphometrical analysis methods and immunohistochemical detection of M1- and M2-macrophages. A collagen membrane was used as the control material. The results of the present study demonstrate that the tissue response to the dPTFE membrane involves inflammatory macrophages, but comparable cell numbers were also detected in the implant beds of the control collagen membrane, which is known to be biocompatible. Although these data indicate that the analyzed dPTFE membrane is not fully bioinert, but its biocompatibility is comparable to collagen-based membranes. Based on its optimal biocompatibility, the novel dPTFE barrier membrane may optimally support bone healing within the context of guided bone regeneration (GBR).

scholarly journals The Release of the Bromodomain Ligand N,N-Dimethylacetamide Adds Bioactivity to a Resorbable Guided Bone Regeneration Membrane in a Rabbit Calvarial Defect Model

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 501 ◽  
Author(s):  
Barbara Siegenthaler ◽  
Chafik Ghayor ◽  
Nisarat Ruangsawasdi ◽  
Franz E. Weber
Keyword(s):  
Drug Delivery ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Good Combination ◽  
Calvarial Defect ◽  
Delivery Vehicle ◽  
Defect Model ◽  
Therapeutic Benefits ◽  
Biodegradable Poly

N,N-Dimethylacetamide (DMA) is FDA approved as an excipient and is used as drug-delivery vehicle. Due to its amphipathic nature and diverse bioactivities, it appears to be a good combination of biodegradable poly-lactide-co-glycolide (PLGA)-based guided bone regeneration membranes. Here we show that the solvent DMA can be loaded to PLGA membranes by different regimes, leading to distinct release profiles, and enhancing the bone regeneration in vivo. Our results highlight the potential therapeutic benefits of DMA in guided bone regeneration procedures, in combination with biodegradable PLGA membranes.

scholarly journals In Vivo Efficacy of Neutrophil-Mediated Bone Regeneration Using a Rabbit Calvarial Defect Model

2021 ◽  
Vol 22 (23) ◽  
pp. 13016
Author(s):  
Thanuja D. K. Herath ◽  
Leonardo Saigo ◽  
Benoit Schaller ◽  
Anis Larbi ◽  
Swee Hin Teoh ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Volume Fraction ◽  
Bone Defects ◽  
Bone Volume ◽  
Surgical Removal ◽  
Calvarial Defect ◽  
Bone Area ◽  
Defect Model ◽  
In Vivo Efficacy

Reconstruction of bone due to surgical removal or disease-related bony defects is a clinical challenge. It is known that the immune system exerts positive immunomodulatory effects on tissue repair and regeneration. In this study, we evaluated the in vivo efficacy of autologous neutrophils on bone regeneration using a rabbit calvarial defect model. Methods: Twelve rabbits, each with two surgically created calvarial bone defects (10 mm diameter), were randomly divided into two groups; (i) single application of neutrophils (SA-NP) vs. SA-NP control, and (ii) repetitive application of neutrophils (RA-NP) vs. RA-NP control. The animals were euthanized at 4 and 8 weeks post-operatively and the treatment outcomes were evaluated by micro-computed tomography, histology, and histomorphometric analyses. Results: The micro-CT analysis showed a significantly higher bone volume fraction (bone volume/total volume) in the neutrophil-treated groups, i.e., median interquartile range (IQR) SA-NP (18) and RA-NP (24), compared with the untreated controls, i.e., SA-NP (7) and RA-NP (14) at 4 weeks (p < 0.05). Similarly, new bone area fraction (bone area/total area) was significantly higher in neutrophil-treated groups at 4 weeks (p < 0.05). Both SA-NP and RA-NP had a considerably higher bone volume and bone area at 8 weeks, although the difference was not statistically significant. In addition, immunohistochemical analysis at 8 weeks revealed a higher expression of osteocalcin in both SA-NP and RA-NP groups. Conclusions: The present study provides first hand evidence that autologous neutrophils may have a positive effect on promoting new bone formation. Future studies should be performed with a larger sample size in non-human primate models. If proven feasible, this new promising strategy could bring clinical benefits for bone defects to the field of oral and maxillofacial surgery.

scholarly journals Silk Protein-Based Membrane for Guided Bone Regeneration

2018 ◽  
Vol 8 (8) ◽  
pp. 1214 ◽  
Author(s):  
Kwang-Jun Kwon ◽  
Hyun Seok
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Guided Bone Regeneration ◽  
Silk Protein ◽  
Regeneration Ability ◽  
Barrier Membrane

Silk derived from the silkworm is known for its excellent biological and mechanical properties. It has been used in various fields as a biomaterial, especially in bone tissue engineering scaffolding. Recently, silk protein-based biomaterial has been used as a barrier membrane scaffolding for guided bone regeneration (GBR). GBR promotes bone regeneration in bone defect areas using special barrier membranes. GBR membranes should have biocompatibility, biodegradability, cell occlusion, the mechanical properties of space-making, and easy clinical handling. Silk-based biomaterial has excellent biologic and mechanical properties that make it a good candidate to be used as GBR membranes. Recently, various forms of silk protein-based membranes have been introduced, demonstrating excellent bone regeneration ability, including osteogenic cell proliferation and osteogenic gene expression, and promoting new bone regeneration in vivo. In this article, we introduced the characteristics of silk protein as bone tissue engineering scaffolding and the recent application of such silk material as a GBR membrane. We also suggested future studies exploring additional uses of silk-based materials as GBR membranes.

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