scholarly journals Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells

Materials ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 227
Author(s):  
Carolina Oliver-Urrutia ◽  
Raúl Rosales Ibañez ◽  
Miriam V. Flores-Merino ◽  
Lucy Vojtova ◽  
Jakub Salplachta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Oral Mucosa ◽  
Three Dimensional ◽  
Rheological Behaviour ◽  
Elastic Solid ◽  
Porous Network ◽  
Pore Connectivity ◽  
Broad Size Distribution ◽  
Negative Effect

This work shows the synthesis of a polyvinylpyrrolidone (PVP) hydrogel by heat-activated polymerization and explores the production of hydrogels with an open porous network by lyophilisation to allow the three-dimensional culture of human oral mucosa stem cells (hOMSCs). The swollen hydrogel showed a storage modulus similar to oral mucosa and elastic solid rheological behaviour without sol transition. A comprehensive characterization of porosity by scanning electron microscopy, mercury intrusion porosimetry and nano-computed tomography (with spatial resolution below 1 μm) showed that lyophilisation resulted in the heterogeneous incorporation of closed oval-like pores in the hydrogel with broad size distribution (5 to 180 μm, d50 = 65 μm). Human oral mucosa biopsies were used to isolate hOMSCs, expressing typical markers of mesenchymal stem cells in more than 95% of the cell population. Direct contact cytotoxicity assay demonstrated that PVP hydrogel have no negative effect on cell metabolic activity, allowing the culture of hOMSCs with normal fusiform morphology. Pore connectivity should be improved in future to allow cell growth in the bulk of the PVP hydrogel.

scholarly journals Characterization of Dental Epithelial Stem Cells from the Mouse Incisor with Two-Dimensional and Three-Dimensional Platforms

2013 ◽  
Vol 19 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Miquella G. Chavez ◽  
Wenli Yu ◽  
Brian Biehs ◽  
Hidemitsu Harada ◽  
Malcolm L. Snead ◽  
...  
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Epithelial Stem Cells ◽  
Mouse Incisor

scholarly journals Fabrication and Multiscale Structural Properties of Interconnected Porous Biomaterial for Tissue Engineering by Freeze Isostatic Pressure (FIP)

2018 ◽  
Vol 9 (3) ◽  
pp. 51 ◽  
Author(s):  
Mythili Prakasam ◽  
Ali Chirazi ◽  
Grzegorz Pyka ◽  
Anna Prokhodtseva ◽  
Daniel Lichau ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Strength ◽  
Cell Attachment ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
High Strength ◽  
Processing Parameters ◽  
Fabrication Technology ◽  
Porous Network ◽  
Pore Connectivity

Biomaterial for tissue engineering is a topic of huge progress with a recent surge in fabrication and characterization advances. Biomaterials for tissue engineering applications or as scaffolds depend on various parameters such as fabrication technology, porosity, pore size, mechanical strength, and surface available for cell attachment. To serve the function of the scaffold, the porous biomaterial should have enough mechanical strength to aid in tissue engineering. With a new manufacturing technology, we have obtained high strength materials by optimizing a few processing parameters such as pressure, temperature, and dwell time, yielding the monolith with porosity in the range of 80%–93%. The three-dimensional interconnectivity of the porous media through scales for the newly manufactured biomaterial has been investigated using newly developed 3D correlative and multi-modal imaging techniques. Multiscale X-ray tomography, FIB-SEM Slice & View stacking, and high-resolution STEM-EDS electronic tomography observations have been combined allowing quantification of morphological and geometrical spatial distributions of the multiscale porous network through length scales spanning from tens of microns to less than a nanometer. The spatial distribution of the wall thickness has also been investigated and its possible relationship with pore connectivity and size distribution has been studied.

scholarly journals Characterization of Three-Dimensional Retinal Tissue Derived from Human Embryonic Stem Cells in Adherent Monolayer Cultures

2015 ◽  
Vol 24 (23) ◽  
pp. 2778-2795 ◽  
Author(s):  
Ratnesh K. Singh ◽  
Ramya K. Mallela ◽  
Pamela K. Cornuet ◽  
Aaron N. Reifler ◽  
Andrew P. Chervenak ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Retinal Tissue ◽  
Monolayer Cultures

scholarly journals Characterization of a Three-Dimensional Mucosal Equivalent: Similarities and Differences with Native Oral Mucosa

2012 ◽  
Vol 195 (3) ◽  
pp. 185-196 ◽  
Author(s):  
Wendy M.W. Tra ◽  
Johan W. van Neck ◽  
Steven E.R. Hovius ◽  
Gerjo J.V.M. van Osch ◽  
Soledad Perez-Amodio
Keyword(s):  
Oral Mucosa ◽  
Three Dimensional ◽  
Similarities And Differences

scholarly journals Three-Dimensional Imaging in Stem Cell-Based Researches

2021 ◽  
Vol 8 ◽  
Author(s):  
Fariborz Nowzari ◽  
Huimei Wang ◽  
Arezoo Khoradmehr ◽  
Mandana Baghban ◽  
Neda Baghban ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
3D Imaging ◽  
Cell Imaging ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
3D Methods ◽  
Regenerative Therapies ◽  
Detailed Imaging

Stem cells have an important role in regenerative therapies, developmental biology studies and drug screening. Basic and translational research in stem cell technology needs more detailed imaging techniques. The possibility of cell-based therapeutic strategies has been validated in the stem cell field over recent years, a more detailed characterization of the properties of stem cells is needed for connectomics of large assemblies and structural analyses of these cells. The aim of stem cell imaging is the characterization of differentiation state, cellular function, purity and cell location. Recent progress in stem cell imaging field has included ultrasound-based technique to study living stem cells and florescence microscopy-based technique to investigate stem cell three-dimensional (3D) structures. Here, we summarized the fundamental characteristics of stem cells via 3D imaging methods and also discussed the emerging literatures on 3D imaging in stem cell research and the applications of both classical 2D imaging techniques and 3D methods on stem cells biology.

Characterization of photosystem II with the atomic-force microscope

1992 ◽  
Vol 50 (2) ◽  
pp. 1146-1147
Author(s):  
Kathleen M. Marr ◽  
Mary K. Lyon
Keyword(s):  
Photosystem Ii ◽  
Atomic Force Microscope ◽  
Three Dimensional ◽  
Biologically Active ◽  
Atomic Force ◽  
Freeze Etching ◽  
Image Averaging ◽  
Oxygen Evolving ◽  
Averaging Techniques

Photosystem II (PSII) is different from all other reaction centers in that it splits water to evolve oxygen and hydrogen ions. This unique ability to evolve oxygen is partly due to three oxygen evolving polypeptides (OEPs) associated with the PSII complex. Freeze etching on grana derived insideout membranes revealed that the OEPs contribute to the observed tetrameric nature of the PSIl particle; when the OEPs are removed, a distinct dimer emerges. Thus, the surface of the PSII complex changes dramatically upon removal of these polypeptides. The atomic force microscope (AFM) is ideal for examining surface topography. The instrument provides a topographical view of individual PSII complexes, giving relatively high resolution three-dimensional information without image averaging techniques. In addition, the use of a fluid cell allows a biologically active sample to be maintained under fully hydrated and physiologically buffered conditions. The OEPs associated with PSII may be sequentially removed, thereby changing the surface of the complex by one polypeptide at a time.

Sign in / Sign up

Export Citation Format

Share Document