scholarly journals Inhibitory Effect of Astaxanthin on Testosterone-Induced Benign Prostatic Hyperplasia in Rats

Marine Drugs ◽  
2021 ◽  
Vol 19 (12) ◽  
pp. 652
Author(s):  
Liping Wang ◽  
Yiwen Hou ◽  
Rong Wang ◽  
Qi Pan ◽  
Debao Li ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Secretory Granules ◽  
Inhibitory Effect ◽  
Prostatic Hyperplasia ◽  
Ventral Prostate ◽  
Control Group ◽  
Sham Operation ◽  
Sod Activity ◽  
Model Control ◽  
Model Control Group

This study investigates the inhibitory effect of astaxanthin (AST) on testosterone-induced benign prostatic hyperplasia (BPH) in rats. Except for the sham operation, BPH model rats were randomly assigned to five groups: the BPH model control rats, AST-treated BPH model rats (20 mg/kg, 40 mg/kg, and 80 mg/kg), and epristeride (EPR)-treated BPH model rats. After treatment, as compared with the BPH model control rats, the prostate and ventral prostate weights of the AST-treated rats decreased, while there was a marked decline in the 80 mg/kg AST-treated rats. The same effect was also observed in the prostate index and ventral prostate index. The proliferation characteristics of epithelia observed in the BPH model control group were gradually alleviated in the AST-treated rats. As compared with the BPH model control rats, lower epithelial thicknesses of prostates and fewer secretory granules in epithelia were observed in the AST-treated rats. The superoxide dismutase (SOD) activity of prostates increased in all the AST-treated rats with a significant increase in the 40 mg/kg and 80 mg/kg AST-treated rats. The testosterone (T) and dihydrotestosterone (DHT) levels of prostates in the AST-treated groups were lower than those in the BPH model control group, and a significant decline was found in the T level of prostates in the 40 g/kg and 80 mg/kg AST-treated rats and the DHT level of prostates in the 40 mg/kg AST-treated rats. These results indicate that AST might have an inhibitory effect on T-induced BPH in rats, possibly due to SOD activity regulation and T and DHT levels.

scholarly journals Effect of Paecilomyces tenuipes Extract on Testosterone-Induced Benign Prostatic Hyperplasia in Sprague–Dawley Rats

2019 ◽  
Vol 16 (19) ◽  
pp. 3764 ◽  
Author(s):  
Choi ◽  
Kim ◽  
Fan ◽  
Tang ◽  
Hwang ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Rat Model ◽  
Testosterone Propionate ◽  
Inhibitory Effect ◽  
Prostatic Hyperplasia ◽  
Control Group ◽  
Lncap Cells ◽  
Prostate Cell ◽  
Expression Levels ◽  
Prostate Size

: Benign prostatic hyperplasia (BPH) is one of the major public health concerns, which has a high prevalence rate and causes significant decline in men's quality of life. BPH is highly related to sexual hormone metabolism and aging. In particular, dihydrotestosterone (DHT), to which testosterone is modified by 5α-reductase (5AR), has a significant effect on BPH development. DHT binds to an androgen receptor (AR) and steroid receptor coactivator 1 (SRC-1); then, it induces the proliferation of a prostate cell and expression of prostate specific antigen (PSA). Paecilomyces tenuipes (P. tenuipes) is a mushroom that has been popularized by the artificial cultivation of fruiting bodies based on silkworms by researchers from the Republic of Korea. In a previous study, we identified the effect of PE on PSA mRNA expression in LNCaP cells. This suggests that PE may have an inhibitory effect on androgen signaling. Therefore, we confirmed the expression of androgen signaling-related factors, such as AR, SRC-1, and PSA in LNCaP. Furthermore, we confirmed the androgen signaling inhibitory effect of PE using the testosterone propionate (TP)-induced BPH rat model. A BPH rat model was established with a four-week treatment of daily subcutaneous injections of testosterone propionate (TP, 3 mg/kg) dissolved in corn oil after castration. The rats in the treatment group were orally gavaged P. tenuipes extract (PE), finasteride (Fi), or saw palmetto extract (Saw) with TP injection. DHT induced an increase in the expression levels of AR, SRC-1, and PSA proteins in LNCaP cells. On the contrary, the PE treatment reduced the expression levels. In vivo, the BPH group showed an increase in prostate size compared with the control group. The PE gavaged group showed a decrease in prostate size compared with the BPH group. In addition, the protein expressions of AR, 5AR2, and PSA were significantly lower in the PE gavaged group than BPH group in prostate tissue. These results suggest the beneficial effects of PE on BPH via the modulation of AR signaling pathway.

Eldecalcitol Plays a Role in Postmenopausal Osteoporosis through Mir-151a-3p/Socs5 Pathway

2021 ◽  
Vol 7 (5) ◽  
pp. 3934-3941
Author(s):  
Hongqing Zhang ◽  
Li Jia ◽  
Danzhi Li
Keyword(s):  
Postmenopausal Osteoporosis ◽  
Cell Activity ◽  
Control Group ◽  
Sham Operation ◽  
Model Group ◽  
Mineral Density ◽  
Specific Mechanism ◽  
Sham Operation Group ◽  
Model Control ◽  
Model Control Group

Objective: This study set out to explore the specific mechanism of Eldecalcitol in postmenopausal osteoporosis (PMOP) and its relationship with miR-151a-3p/SOCS5 pathway. Methods: Forty-five rats were randomly and equally divided into sham operation group (SOG), model control group (MCG) and Eldecalcitol group (EG). miR-151a-3p, SOCS5 and bone mineral density (BMD) levels in each group were detected. MC3T3-E1 cells were modeled and divided into control group (CG), model group (MG) and EG. miR-151a-3p-inhibitor and pcDNA3.1-SOCS were transfected into model cells. miR-151 A-3P, SOCS5, RANKL and OPG levels as well as cell activity of cells in each group were observed. Results: Eldecalcitol intervention in rats can reduce BMD reduction caused by PMOP, reduce the miR-151a-3p level and increase the SOCS5 level. Cell experiments found that Eldecalcitol intervention can improve cell activity, inhibit the miR-151a-3p level and promote the SOCS5 expression, all of which can improve bone resorption of model cells, increase cell activity, inhibit the RANKL level and promote the OPG level. Conclusion: Eldecalcitol plays a role in PMOP by inhibiting miR-151a-3p and promoting the SOCS5 level.

Iodine Bonded with Milk Protein Inhibits Benign Prostatic Hyperplasia Development in Rats

2019 ◽  
Vol 19 (13) ◽  
pp. 1627-1632
Author(s):  
Vladimir G. Bespalov ◽  
Valerii A. Alexandrov ◽  
Grigory V. Tochilnikov ◽  
Dmitrii Е. Lukin ◽  
Nadezhda T. Zhilinskaya ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Sunflower Oil ◽  
Milk Protein ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Prostatic Hyperplasia ◽  
The Body ◽  
Control Group ◽  
Male Wistar Rats ◽  
Conducting Research

Background: There is some evidence that Benign Prostatic Hyperplasia (BPH) may increase the risk of developing prostate cancer, so conducting research on effective BPH inhibitors is important. Objective: This research studied the inhibitory effect of Iodized Serum Milk Protein (ISMP) on BPH in rats. ISMP is a concentrate of lactic protein containing 2.2% iodine. Methods: Male Wistar rats, aged 18 months, were used. In the intact control group, sunflower oil was administered intragastrically by gavage. In 36 rats, BPH was induced by surgical castration, followed by subcutaneous injections of prolonged testosterone - omnadren, 25mg/kg every other day (7 administrations). One group of rats served as BPH-control. ISMP and finasteride (positive control), dissolved in sunflower oil, were administered to rats intragastrically daily at a dose of 200μg/kg and 5mg/kg, respectively, for 4 weeks starting immediately after castration. Results: ISMP inhibited the development of BPH in rats, significantly reducing the mass of the prostate and its parts (except for the anterior lobes) by 1.1-1.3 times and the prostatic index (the ratio of prostate weight to the body weight) - by 1.3-1.4 times. Finasteride inhibited the development of BPH, and its activity was higher (by 1.1-1.3 times) than in ISMP. : Histological analysis of the prostate showed fewer pronounced morphological hyperplasia signs in animals treated with ISMP or finasteride. Conclusion: The iodine-containing preparation ISMP has the ability to inhibit the development of BPH in rats although its activity is somewhat lower than that of finasteride.

scholarly journals Inhibitory effect of low-molecular-weight peptides (0–3 kDa) from Spirulina platensis on H2O2-induced oxidative damage in L02 human liver cells

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jun Ma ◽  
Xiankun Zeng ◽  
Min Zhou ◽  
Le Cheng ◽  
Difeng Ren
Keyword(s):  
Molecular Weight ◽  
Oxidative Damage ◽  
Spirulina Platensis ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Control Group ◽  
Low Molecular Weight ◽  
Sod Activity ◽  
Liver Health ◽  
L02 Cells

AbstractSpirulina platensis protein hydrolysates were prepared by digesting protein extracts with papain, and the hydrolysates were separated into 30, 10, and 3 kDa weights using membrane ultrafiltration. The 0–3 kDa low-molecular-weight Spirulina peptides (LMWSPs) proved the highest chemical antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, hydroxyl radical (·OH) scavenging activities and total antioxidant capacity. Cellular antioxidant ability of LMWPs fractions against 2000 μg/mL H2O2 induced oxidative damage of L02 cells were investigated. The MTT assay results displayed that LMWSPs at different concentrations (0–1000 μg/mL) had proliferation effect on the L02 cells and that treatment of the L02 cells with the 1000 μg/mL LMWSPs (0–3 kDa) significantly prevented H2O2-induced oxidative damage compared with control cells. Moreover, the 2′,7′-dichlorofluorescein diacetate (DCFH-DA) fluorescent probe assay showed that the levels of ROS and NO were significantly lower in the experimental group that was treated with the peptides for 24 h than in the control group. Furthermore, using the corresponding kits, the treatment inhibited the reduction of SOD activity and the increase of MDA contents in the L02 cells. Therefore, LMWSPs (0–3 kDa) may have potential applications in antioxidant and liver health products.

SIGNIFICANCE OF NON-INVASIVE SONO-MORPHOLOGICAL MARKERS IN THE EVALUATION OF BLADDER OUTLET OBSTRUCTION AND ACUTE URINARY RETENTION SECONDARY TO BENIGN PROSTATIC HYPERPLASIA

2021 ◽  
pp. 5-9
Author(s):  
Prasenjit Bhowmik ◽  
Soumendranath Mandal ◽  
Gaurav Sharma ◽  
Bandhan Bahal ◽  
Prashant Gupta ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Diagnostic Accuracy ◽  
Urinary Retention ◽  
Bladder Outlet Obstruction ◽  
Acute Urinary Retention ◽  
Bladder Wall ◽  
Outlet Obstruction ◽  
Threshold Value ◽  
Prostatic Hyperplasia ◽  
Control Group

Objectives: To dene the diagnostic accuracy of intravesical protrusion of prostate (IPP), bladder wall thickness (BWT) and prostate volume (PV) in diagnosis of bladder outlet obstruction (BOO) and prediction of future acute urinary retention (AUR). A prospective Materials and methods: study of 127 patients, presenting with lower urinary tract symptoms suggestive of benign prostatic hyperplasia (BPH) were enrolled with 50 patients among them manifesting AUR. 35 normal persons were selected as control group. After inclusion, all patients underwent transabdominal ultrasound and pressure ow urodynamic study (UDS). UDS parameter, BOO index (BOOI) were used as a reference standard for stratifying the patients into two groups, BOO and non-BOO. The sono-morphological parameters, IPP, BWT and PV were used to compare between these two groups with calculating the diagnostic accuracy for each individual index. IPP, BWT Results: and PV had statistically signicant difference between group 1 (BOOI < 40) and 2 ( BOOI > 40) with strong correlation with BOOI. The Pearson's correlation coefcient (r) for IPP, BWT and PV were 0.762, 0.702 and 0.660 respectively. The AUC for IPP, BWT and PV were 0.824, 0.786 and 0.650 with highest accuracy for IPP (79.2%) at cutoff value of 7 mm. Using the same threshold value, IPP had higher statistical difference than BWT in predicting AUR with similar diagnostic accuracy of IPP and BWT together. IPP and BWT in conjunction with PV in place Conclusions: of UDS had good clinical utility in diagnosis of BOO due to BPH and future AUR prediction.

scholarly journals Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic Hyperplasia

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2638 ◽  
Author(s):  
Bo-Ram Jin ◽  
Hyo-Jung Kim ◽  
Sang-Kyun Park ◽  
Myoung-Seok Kim ◽  
Kwang-Ho Lee ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Prostatic Hyperplasia ◽  
Nuclear Antigen ◽  
Control Group ◽  
Over Expression ◽  
Alternative Medicines ◽  
Androgenic Hormone ◽  
Vitro Model ◽  
Proliferating Cell

Benign prostatic hyperplasia (BPH), an age-dependent disorder with a prevalence percentage of 60% in the 60s, has been found to involve an androgenic hormone imbalance that causes confusion between cell apoptosis and proliferation. Because general medications for BPH treatment have undesirable side effects, the development of effective alternative medicines has been considered. HBX-5 is a newly developed formula with the aim of improving BPH, and is composed of nine medicinal herbs. BPH was induced in the rats by intramuscular injection of testosterone propionate after castration. Rats were divided into six groups, and the efficacy of HBX-5 on testosterone-induced BPH in rats was estimated. In addition, RWPE-1 and WPMY-1 cells were used to demonstrate the effect of HBX-5 on BPH in vitro model. Compared with the control group, HBX-5 administration group suppressed BPH manifestations, such as excessive development of prostate, and increase of serum dihydrotestosterone and 5α-reductase concentrations. Furthermore, immunohistochemistry analysis revealed that HBX-5 significantly decreased the expression of androgen receptor (AR) and proliferating cell nuclear antigen (PCNA). In addition, results of RWPE-1 and WPMY-1 cells showed that HBX-5 inhibited the over-expression of AR and PSA in DHT-induced prostate hyperplastic microenvironments.

scholarly journals Circulating miRNAs as Biomarkers for Prostate Cancer Diagnosis in Subjects with Benign Prostatic Hyperplasia

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Wei Jin ◽  
Xiang Fei ◽  
Xia Wang ◽  
Fangjie Chen ◽  
Yan Song
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Statistical Tests ◽  
Specific Antigen ◽  
Area Under The Curve ◽  
Prostatic Hyperplasia ◽  
Control Group ◽  
Circulating Mirnas ◽  
Noninvasive Biomarker ◽  
Novel Biomarkers

Body fluids often contain freely circulating nucleic acids, many of which can be exploited as noninvasive tools for the diagnosis of cancer as well as for clinical prognostication. Identifying microRNAs (miRNAs) in subjects’ blood with various malignancies means that they can serve as novel biomarkers for prostate cancer (PCa) diagnosis. This study analyzed serum-circulating miRNAs as a noninvasive biomarker in subjects with PCa and subjects with benign prostatic hyperplasia (BPH). In total, 31 PCa subjects and 31 BPH subjects were included, with the BPH group serving as the control group. RT-qPCR was used to quantify the levels of 10 miRNAs, which included miR-18a, miR-34a, miR-106b, miR-183, miR-200a, miR-301a, miR-141, miR-182, miR-200b, and miR-375 in serum. Statistical tests were used to assess the relationship between the levels of miRNAs and the clinicopathological data. A significant increase was observed in the relative expression ratios of miR-141, miR-182, miR-200b, and miR-375 (1.89-, 2.09-, 2.41-, and 2.27-folds, respectively) in the PCa group when compared to the BPH group. Based on the receiver operating characteristic (ROC) analysis, the largest area under the curve (AUC), 0.923, was associated with the miR-200b group, indicating effective diagnostic properties for this biomarker. A correlation was observed between total prostate-specific antigen (TPSA) and the relative levels of miR-141, miR-182, miR-200b, and miR-375. The Gleason score and the miR-200b expression level were also correlated. These results are consistent with previous studies regarding the possibility of differentiating between PCa subjects and healthy controls based on the detection of miRNA. The findings attest to a distinctive expression profile of miRNA that is detectable in the blood of PCa subjects, thereby confirming the role of miRNAs as diagnostic biomarkers for PCa.

scholarly journals Increased Antioxidant Quality Versus Lower Quantity Of High Density Lipoprotein In Benign Prostatic Hyperplasia

2015 ◽  
Vol 34 (4) ◽  
pp. 455-459
Author(s):  
Ozgur Aydin ◽  
Hamit Yasar Ellidag ◽  
Esin Eren ◽  
Nurullah Ay ◽  
Soner Yalçınkaya ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Total Antioxidant Status ◽  
Prostatic Hyperplasia ◽  
High Density ◽  
Paraoxonase 1 ◽  
Control Group ◽  
Laboratory Staff ◽  
Total Antioxidant

SummaryBackground:Oxidative stress may be involved in the pathogenesis of every human disease. To understand its possible role in benign prostatic hyperplasia (BPH), we measured the overall oxidative status of patients with BPH and the serum activity of the high density lipoprotein (HDL)-related antioxidant enzymes paraoxonase 1 (PON1) and arylesterase (ARE).Methods:Fifty-six urology outpatient clinic patients with BPH (mean age 64±8.6 years) were prospectively included in the study. Forty volunteer healthy controls from the laboratory staff (mean age 62±10 years) were enrolled for comparison. Serum total antioxidant status (TAS), total oxidant status (TOS), PON1, ARE, and HDL levels were measured by commercially available, ready-to-use kits.Results:Serum TAS and HDL levels were significantly lower in the BPH group than in the control group (P=0.004 and P=0.02, respectively). No significant between-group differences were observed for TOS levels or PON1 and ARE enzyme activities (P=0.30, P=0.89, and P=0.74, respectively). In the BPH group, the calculated parameters PON1/HDL and ARE/HDL were significantly higher (P=0.02 and P=0.04, respectively).Conclusions:Our findings agree with the previous reports of impaired oxidant/antioxidant balance in BPH patients. The activities of HDL-related enzymes between groups with significantly different HDL levels may be deceptive; adjusted values may help to reach more accurate conclusions.

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