Heterofucans from the Brown Seaweed Canistrocarpus cervicornis with Anticoagulant and Antioxidant Activities

Objective: Phytochemical is naturally present in the seaweeds which biologically play a significant role. The intention of this study was designed to screen the phytochemical constituents and antimicrobial potential of selected seaweed collected from Rameshwaram and Tuticorin Southern coast of India.Methods: The present study investigated the presence of phytochemical constituents and also total phenol, total carbohydrate and total protein quantity of the brown seaweed. Dictyopteris delicatula, Padina gymnospora, Acanthophora spicifera, Portieria hornemannii and Ulva faciata were extracted with solvents having different polarities like methanol, ethanol, chloroform and water and screened for the phytochemical constituents, total phenol, total carbohydrate, total protein and DPPH with standard procedure. The antibacterial activities of the seaweeds were examined by agar well diffusion method.Results: Among the five seaweeds, U. faciata showed the maximum number of active constituents in the methanol extract likewise P. gymnospora was found to have a number of diligent compounds in ethanol extract. A. spicifera showed minimum compounds in ethanol as well as chloroform extract. Moreover A. spicifera, P. hornemannii have shown the superior quantity of protein and carbohydrate when compared to other species. The scavenging activity of methanol extracts at 5 mg/ml concentration P. hornemannii shows 18.2% and A. spicifera possess 17.1%. In the antibacterial activity, methanol extracts of all the seaweed showed a potential inhibitory activity against B. cereus and P. aeruginosa compared to other pathogens.Conclusion: The crude extract of seaweed manifest preferable antimicrobial and antioxidant activities, hence in the future, it would be good if it is further taken for treatment of human diseases or as new antimicrobial agents to replace synthetic antimicrobial agents.

Download Full-text

Chemical defenses of the tropical marine seaweed Canistrocarpus cervicornis against herbivory by sea urchin

Brazilian Journal of Oceanography ◽

10.1590/s1679-87592010000300004 ◽

2010 ◽

Vol 58 (3) ◽

pp. 213-218 ◽

Cited By ~ 16

Author(s):

Éverson Miguel Bianco ◽

Valéria Laneuville Teixeira ◽

Renato Crespo Pereira

Keyword(s):

Sea Urchin ◽

Chemical Properties ◽

Brown Seaweed ◽

Chemical Defenses ◽

Lytechinus Variegatus ◽

Brown Seaweeds ◽

Natural Concentration ◽

Compound Type ◽

Defensive Chemical ◽

Canistrocarpus Cervicornis

This paper reports on the defensive chemical properties of the marine tropical brown seaweed Canistrocarpus cervicornis against herbivory. A natural concentration of dichloromethane crude extract (DCE) obtained from this seaweed significantly inhibited feeding by the sea urchin Lytechinus variegatus. The major metabolite isolated from this active DCE extract was identified as the (4R,7R,14S)-4α,7α-diacetoxy-14-hydroxydolast-1(15),8-diene that strongly inhibited feeding by the same sea urchin. This result suggests that the dolastane diterpenes class may constitute the defensive system of C. cervicornis against herbivory, and probably also of that of other brown seaweeds endowed with a biosynthetic pathway capable of producing compounds of the dolastane-type, a typical skeleton found in Dyctioteae species worldwide. This is the first report showing this compound-type (dolastane diterpenes) as a chemical defense against herbivory in marine seaweeds. This study constitutes an additional report broadening the known spectrum of action and roles of secondary metabolites of the C. cervicornis and Dyctioteae species.

Download Full-text

Antiskin Cancer and Antioxidant Activities of Formulated Agar from Brown Seaweed Laminaria digitata (Hudson) in Dimethyl Benzanthracene-Induced Swiss Albino Mice

International Journal of Polymer Science ◽

10.1155/2021/9930777 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Jeneesha George ◽

A. Thabitha ◽

N. Vignesh ◽

V. Manigandan ◽

R. Saravanan ◽

...

Keyword(s):

Antioxidant Activities ◽

Histopathological Examination ◽

Brown Seaweed ◽

Cancer Control ◽

In Vitro Cytotoxicity ◽

Control Group ◽

High Dose ◽

Laminaria Digitata ◽

Group 2

This study explores the antiskin cancer effect of formulated agar (FA) from Laminaria digitata on dimethyl benzanthracene- (DMBA-) induced skin cancer mice. The agar was extracted and formulated (emulgel), and FA was biochemically characterized. The in vitro cytotoxicity of FA was tested using NTT 3T3 mice fibroblast cells. The mice were divided into 5 groups: group 1 served as control mice, group 2 mice were considered as DMBA-induced cancer control, group 3 mice were FA pretreated (low dose) + DMBA-induced mice, group 4 mice were FA pretreated (high dose) + DMBA-induced mice, and group 5 were positive control + DMBA-induced mice. The behaviour and biochemical markers of cancer were significantly decreased in group 2 (DMBA-induced) mice, which were brought to near normalcy by FA pretreated mice (groups 3 and 4). The levels of p53 and keratin were significantly elevated in group 2 mice and these levels were decreased in 3 and 4 mice as well. The histopathological examination of DMBA-induced mice was shown degenerated cervical patches in the skin, cirrhosis in liver, oedema in the renal tissue, and swollen and damage in cardiac tissue, which were reduced for the mice applied with FA. This confirms that FA pretreatment offered potential antiskin cancer property.

Download Full-text

Measurement of Antioxidant Activities and Phenolic and Flavonoid Contents of the Brown Seaweed Sargassum horneri: Comparison of Supercritical CO2 and Various Solvent Extractions

Fisheries and aquatic sciences ◽

10.5657/fas.2015.0123 ◽

2015 ◽

Vol 18 (2) ◽

pp. 123-130 ◽

Cited By ~ 1

Author(s):

Shipeng Yin ◽

Hee-Chul Woo ◽

Jae-Hyung Choi ◽

Yong-Beom Park ◽

Byung-Soo Chun

Keyword(s):

Antioxidant Activities ◽

Brown Seaweed ◽

Sargassum Horneri ◽

Phenolic And Flavonoid

Download Full-text

Effects of deodorisation methods on volatile compounds, chemical properties and antioxidant activities of fucoidan isolated from brown seaweed (Sargassum sp.)

Algal Research ◽

10.1016/j.algal.2017.06.018 ◽

2017 ◽

Vol 25 ◽

pp. 507-515 ◽

Cited By ~ 5

Author(s):

Sharifah Habibah Syed Khalafu ◽

Wan Mustapha Wan Aida ◽

Seng Joe Lim ◽

Mohamad Yusof Maskat

Keyword(s):

Volatile Compounds ◽

Antioxidant Activities ◽

Chemical Properties ◽

Brown Seaweed

Download Full-text

Optimisation of Ultrasound Frequency, Extraction Time and Solvent for the Recovery of Polyphenols, Phlorotannins and Associated Antioxidant Activity from Brown Seaweeds

Marine Drugs ◽

10.3390/md18050250 ◽

2020 ◽

Vol 18 (5) ◽

pp. 250 ◽

Cited By ~ 4

Author(s):

Viruja Ummat ◽

Brijesh K Tiwari ◽

Amit K Jaiswal ◽

Kevin Condon ◽

Marco Garcia-Vaquero ◽

...

Keyword(s):

Total Phenolics ◽

Antioxidant Activities ◽

Low Cost ◽

Brown Seaweed ◽

Biologically Active ◽

Ultrasound Assisted Extraction ◽

Antioxidant Power ◽

Seaweed Species ◽

Time Frequency ◽

Trolox Equivalent

This study investigates ultrasound assisted extraction (UAE) process parameters (time, frequency and solvent) to obtain high yields of phlorotannins, flavonoids, total phenolics and associated antioxidant activities from 11 brown seaweed species. Optimised UAE conditions (35 kHz, 30 min and 50% ethanol) significantly improved the extraction yield from 1.5-fold to 2.2-fold in all seaweeds investigated compared to solvent extraction. Using ultrasound, the highest recovery of total phenolics (TPC: 572.3 ± 3.2 mg gallic acid equivalent/g), total phlorotannins (TPhC: 476.3 ± 2.2 mg phloroglucinol equivalent/g) and total flavonoids (TFC: 281.0 ± 1.7 mg quercetin equivalent/g) was obtained from Fucus vesiculosus seaweed. While the lowest recovery of TPC (72.6 ± 2.9 mg GAE/g), TPhC (50.3 ± 2.0 mg PGE/g) and TFC (15.2 ± 3.3 mg QE/g) was obtained from Laminaria digitata seaweed. However, extracts from Fucus serratus obtained by UAE exhibited the strongest 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity (29.1 ± 0.25 mg trolox equivalent/g) and ferric reducing antioxidant power (FRAP) value (63.9 ± 0.74 mg trolox equivalent/g). UAE under optimised conditions was an effective, low-cost and eco-friendly technique to recover biologically active polyphenols from 11 brown seaweed species.

Download Full-text

ANTIOXIDANT ACTIVITY AND PHENOLIC CONTENTS OF SEVEN BROWN SEAWEED FROM DJIBOUTI COAST

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2019v11i3.34095 ◽

2019 ◽

pp. 42-44

Author(s):

ABDIRAHMAN ELMI FOURREH ◽

FATOUMA MOHAMED ABDOUL-LATIF ◽

MOUSTAPHA NOUR IBRAHIM ◽

ALI MERITO ALI

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Dry Matter ◽

Phenolic Content ◽

Antioxidant Activities ◽

Biological Activities ◽

Brown Seaweed ◽

Antioxidant Potential ◽

Phenolic Contents ◽

Dpph Method

Objective: This study focuses on the antioxidant potential of Djibouti brown seaweed and their phenolic contents. Methods: We evaluated the antioxidant potential by DPPH method (1,1-diphenyl-2-picrylhydrazyl) and their phenolic contents of seven Djibouti seaweed: Cytoseira myrica, Padina pavonica, Sargassum fluitans, Sargassum ilifolium, Sargassum sp, Turbinaria triquetra and Turbinaria turbinata. Also, we searched the secondary metabolites of these seaweeds. Results: We obtain a higher antioxidant activity at 60,7±0,9 % and a higher phenolic content at 199,01±0,5 μg equivalent phloroglucinol (PGE)/g dry matter for Padina pavonica. A good linear correlation (R2 = 0,898) is observed between the antioxidant activity and the phenolic content of the seaweed studied. Also, two Padina pavonica collected in two different locations have different biochemical concentrations and antioxidant activity, suggesting the influence of the marine environment on the biosynthesis of secondary metabolites and the biological activities of seaweed. The present study shows the presence of tannins, saponosides, flavonoids and steroid-terpenes. Conclusion: The species studied show interesting antioxidant activities and can be consumed to prevent oxidative stress.

Download Full-text

Antilonomic Effects of Brazilian Brown Seaweed Extracts

Natural Product Communications ◽

10.1177/1934578x0900400811 ◽

2009 ◽

Vol 4 (8) ◽

pp. 1934578X0900400

Author(s):

Thaisa Francielle Souza Domingos ◽

Carla Carvalho ◽

Laura de Andrade Moura ◽

Valéria Laneuville Teixeira ◽

Renato Crespo Pereira ◽

...

Keyword(s):

Blood Clotting ◽

Marine Algae ◽

Biological Activities ◽

Brown Seaweed ◽

Human Beings ◽

Coagulation Activity ◽

Marine Algal ◽

Canistrocarpus Cervicornis ◽

Algal Extracts

The aim of this work was to investigate the hemolysis and blood clotting activity of Lomonia obliqua venom and the ability of some Brazilian marine algal extracts ( Canistrocarpus cervicornis, Stypopodium zonale and Dictyota pfaffi) to antagonize such biological activities. L. obliqua caterpillars are dangerous to human beings and envenomation symptoms are characterized by hemorrhagic, hemolytic and blood clotting disorders, and acute renal failure, which sometimes lead to the death of the victims. Through in vitro experiments we have shown that L. obliqua venom is able to clot human plasma and hemolize human erythrocytes and that the coagulation activity of the venom is inhibited by the extracts of C. cervicornis, S. zonale and D. pfaffi. In contrast, C. cervicornis and S. zonale extracts did not inhibit the hemolytic activity of L. oblqua, as did the extract of D. pfaffi. These finding indicate that marine algae may be used as antivenoms or may contribute to the development of compounds with antilonomic effects.

Download Full-text