ANTIOXIDANT ACTIVITY AND PHENOLIC CONTENTS OF SEVEN BROWN SEAWEED FROM DJIBOUTI COAST

Objective: This study focuses on the antioxidant potential of Djibouti brown seaweed and their phenolic contents. Methods: We evaluated the antioxidant potential by DPPH method (1,1-diphenyl-2-picrylhydrazyl) and their phenolic contents of seven Djibouti seaweed: Cytoseira myrica, Padina pavonica, Sargassum fluitans, Sargassum ilifolium, Sargassum sp, Turbinaria triquetra and Turbinaria turbinata. Also, we searched the secondary metabolites of these seaweeds. Results: We obtain a higher antioxidant activity at 60,7±0,9 % and a higher phenolic content at 199,01±0,5 μg equivalent phloroglucinol (PGE)/g dry matter for Padina pavonica. A good linear correlation (R2 = 0,898) is observed between the antioxidant activity and the phenolic content of the seaweed studied. Also, two Padina pavonica collected in two different locations have different biochemical concentrations and antioxidant activity, suggesting the influence of the marine environment on the biosynthesis of secondary metabolites and the biological activities of seaweed. The present study shows the presence of tannins, saponosides, flavonoids and steroid-terpenes. Conclusion: The species studied show interesting antioxidant activities and can be consumed to prevent oxidative stress.

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SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

Jurnal Natural ◽

10.30862/jn.v7i1.678 ◽

2008 ◽

Vol 7 (1) ◽

Author(s):

Bimo Budi Santoso

Keyword(s):

Antioxidant Activity ◽

Medicinal Plants ◽

Antitumor Activity ◽

Traditional Method ◽

Extraction Efficiency ◽

Phenolic Content ◽

Antioxidant Activities ◽

Total Phenolic ◽

Phenolic Contents ◽

Total Phenolic Contents

Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed. The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents, A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R2= 0,77) and methanol (R2 = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.

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Antioxidant Activities and Total Phenolics of Acacia Honey

Czech Journal of Food Sciences ◽

10.17221/1112-cjfs ◽

2009 ◽

Vol 27 (Special Issue 1) ◽

pp. S245-S247 ◽

Cited By ~ 17

Author(s):

M. Krpan ◽

K. Marković ◽

G. Šarić ◽

B. Skoko ◽

M. Hruškar ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Antiradical Activity ◽

Phenolic Content ◽

Antioxidant Activities ◽

Optical Rotation ◽

Total Phenolic ◽

Antioxidant Power ◽

Dpph Method ◽

Acacia Honey

The antioxidant activities and total phenolic content of 30 samples of acacia honey from Croatian territory were analysed. Phenolics were determined by the modified Folin-Ciocalteu method, antiradical activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and potential antioxidant activity using the ferric reducing antioxidant power (FRAP) method. In all samples, physicochemical parameters (water content, electrical conductivity, total reducing sugars, sucrose content, acidity, hydroxymethylfurfural content, prolin content, optical rotation, diastase activity and invertase activity) were measured according to Croatian legislation and International regulatory standards. Honey can be considered as a dietary supplement as it contains some important components including α-tocopherol, ascorbic acid, flavonoids and phenolics. The composition and properties of honey are dependent on floral origins, climatic conditions of the produced area, processing and storage methods. The results of physicochemical analyses showed that all the values of investigated parameters are in agreement with the current legislation. Phenolic content ranged from 31.72 mg/kg to 80.11 mg/kg, antiradical activity expressed as IC50 ranged from 61.28% to 253.47% and antioxidant activity expressed as FRAP value from 6.95 to 142.43. A positive correlation was observed between total phenolic content and antioxidant activity, indicating that phenolic compounds are mainly responsible for the antioxidant power of acacia honey.

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CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF SECONDARY METABOLITES FROM PULICARIA UNDULATA

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i9.12814 ◽

2016 ◽

Vol 8 (9) ◽

pp. 150 ◽

Cited By ~ 6

Author(s):

Taha A. Hussien ◽

Sayed A. El-toumy ◽

Hossam M. Hassan ◽

Mona H. Hetta

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Antioxidant Activities ◽

Structure Activity Relationship ◽

Activity Relationship ◽

Hep G2 ◽

Structure Activity ◽

Dpph Method ◽

Mcf 7

Objective: To evaluate the in vitro cytotoxicity, antioxidant activities and structure-activity relationship of secondary metabolites isolated from Pulicaria undulata.Methods: The methylene chloride-methanol (1:1) extract of the air-dried aerial parts of Pulicaria undulata was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of the isolated compounds were determined on the basis of the extensive spectroscopic analysis, including 1D and 2D NMR and compared with the literature data. The crude extract and the isolated compounds were evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method and cytotoxic assay using human breast cancer (MCF-7) and hepatoma (Hep G2) cell line.Results: Nine secondary metabolites were isolated from Pulicaria undulata in this study. Of which two terpenoidal compounds; 8-epi-ivalbin and 11β, 13-dihydro-4H-xanthalongin 4-O-β-D-glucopyranoside firstly isolated from the genus pulicaria and three flavonoids; eupatolitin, 6-methoxykaempferol, and patulitrin firstly isolated from P. undulata. 6-methoxykaempferol (IC50 2.3 µg/ml) showed the most potent antioxidant activity. The highest cytotoxic effect against MCF-7 and Hep G2 cells was obtained with eupatolitin (IC50 27.6 and 23.5 µg/ml) respectively. The structure-activity relationship was also examined and the findings presented here showed that 3, 5, 7, 4' and 3, 5, 4', 5'-hydroxy flavonoids were potent antioxidant and has cytotoxic activity.Conclusion: Pulicaria undulata is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as antioxidant drug and in the treatment of cancer.

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Evaluation of Secondary Metabolites and Antioxidant Activity of Water, Ethyl Acetate and Hexane Fractions from the Mangrove Young Leaves Sonneratia alba

Chemical Science International Journal ◽

10.9734/csji/2021/v30i230215 ◽

2021 ◽

pp. 23-32

Author(s):

Verly Dotulong ◽

Djuhria Wonggo ◽

Lita A. D. Y. Montolalu

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Secondary Metabolite ◽

Antioxidant Activities ◽

Extraction Methods ◽

Water Fraction ◽

Sonneratia Alba ◽

Dpph Method ◽

Young Leaves

The fractions from young leaves of mangrove Sonneratia alba was studied for its associated secondary metabolites and antioxidant activities. The objective of this study was to determine the secondary metabolite components and antioxidant activity of water, ethyl acetate, and hexane fractions of the young leaves of mangrove S. alba. The fraction was obtained from dry powder of young leaf S.alba using continuous fractionation of crude extracts. The crude extract was attained by 2 extraction methods (soxhlet and maceration) and 2 extraction solvents (methanol and ethanol). Secondary metabolites analyses were qualitatively conducted to detect the presence or absence of phenols, flavonoid, tannin, steroid, triterpenoid and alkaloid. Total phenols were measured using Folin Ciocalteau reagents and gallic acid standard curves whereas antioxidant activity were analyzed using DPPH method (1- 1-diphenil-2-picrihydrasil). Results showed that all fractions contained secondary metabolite components tested. The highest rendement was found in the water fraction fromsoxhletation extract with methanol (6.36±0.29%). The total phenol values were found the highest in the ethylacetatefraction from macerated extract with ethanol (352±9.77 mgGAE/g). Stronger antioxidant activity was also found in ethylacetate fraction as indicated by the small value of IC50 DPPH namely the ethylacetate fraction with soxhletation extract with ethanol (3.43±0.25 µg / mL). The results of this study indicate that the semipolar fraction (ethylacetate fraction) has more potential as a source of natural antioxidants.

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Effect of Geographical and Seasonal Variations on Phenolic Contents and Antioxidant Activity of Aerial Parts of Urtica diocia L.

Current Traditional Medicine ◽

10.2174/2215083804666181012123333 ◽

2019 ◽

Vol 5 (2) ◽

pp. 159-167 ◽

Cited By ~ 2

Author(s):

Vinod Kumar Gautam ◽

Mannu Datta ◽

Ashish Baldi

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Seasonal Variations ◽

Summer Season ◽

Antioxidant Potential ◽

Total Phenolic ◽

Spring Season ◽

Phenolic Contents ◽

Aerial Parts ◽

Phenolic And Flavonoid

Background: Environmental conditions affect the biosynthesis of secondary metabolites as a result of biotic and abiotic factors. In the present study, the effect of different geographical locations and season was studied on total phenolic and flavonoid contents extracted from Urtica dioica Linn. Methodology: The aerial parts of U. dioica collected from Palampur, Shimla and Dharamshala in different seasons were subjected to hydro-alcoholic extraction. Quantitative estimation of total phenolic and flavonoid contents in various extracts was carried out spectrophotometrically. Results: The highest amount of total phenolic (3.987± 0.130) and flavonoid contents (2.847± 0.341) was found in Palampur sample collected in summer season whereas sample collected from Dharamshala in spring season showed the least phenolic contents. In vitro antioxidant activity of all the samples was evaluated by DPPH, NO scavenging and FRPA method. The antioxidant potential was found maximum in the sample collected from Palampur in the summer season, however, the sample collected from Dharamshala in spring season showed the least antioxidant potential. Conclusion: The present study confirms that altitude and seasonal variations significantly affect the levels of secondary metabolites in plant parts.

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Comparative Biochemical Analysis of Secondary Metabolites in Commonly Grown Legume Varieties

Legume Research - An International Journal ◽

10.18805/lr-4217 ◽

2019 ◽

Author(s):

Savita Rani ◽

Sukriti Nehra ◽

M Khabiruddin ◽

Shubham Lamba

Keyword(s):

Free Radical ◽

Secondary Metabolites ◽

Seed Coat ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Total Phenolic ◽

Phenolic Contents ◽

Dpph Method ◽

Whole Seed

In this research study, quantitative determination of phytoconstituents in seed coat, dehusked raw dal, cooked dal and whole seed of locally grown pulses viz. pigeonpea, lentil, desi and kabuli chickpea, and ricebean was carried out. Among the four treatments of all the pulses, highest yield in methanol was found in ricebean. Total phenolic content (mg GAEg-1) varied from 1.72-103.62 in all of the extracts. Similarly, flavonoids (mg CAEg-1) ranged from 1.13 to 33.66 which also found to be reduced in dehulled and cooked extracts. o-Dihydric phenols, hydrophobic and hydrophilic phenols followed order as: seed coat> whole seed> raw dal> cooked dal. Results obtained by DPPH method showed that free radical scavenging capacities ranged from 53.80 to 92.00% while antioxidant activity ranged from 51.00 to 78.60% by FTC method. A highly significant correlation (plessthan0.05) between antioxidant activities/free radical scavenging efficiencies and phenolic contents as well as flavonoids was observed in most of the extracts under study. The results concluded that the pulse seed extracts may be valuable natural source of secondary metabolites for nutraceutical industry.

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Comparative analysis of antioxidant activity and phenolic compounds in the fruits of Aronia spp.

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1360 ◽

2020 ◽

Vol 14 ◽

pp. 393-401

Author(s):

Yulia Vinogradova ◽

Olena Vergun ◽

Olga Grygorieva ◽

Eva Ivanišová ◽

Ján Brindza

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Phenolic Acids ◽

Antioxidant Activities ◽

Biological Activities ◽

Reducing Power ◽

Biologically Active ◽

Polyphenol Compounds ◽

Dpph Method ◽

Ethanol Extracts

Chokeberry (Aronia Medik.) is a non-traditional fruit plant known as a rich source of biologically active compounds and inhibits the numerous biological activities. We compared the antioxidant activity and phenolic compounds of fruits between widely cultivated Aronia mitschurinii (AM-TCH, from Tchekhov district; AM-D, from Dmitrov district; AM-OZ, from Orekhovo-Zuevsky district of Moscow region, Russia) and introduced North American Aronia species (Aronia arbutifolia (AA-M), A. melanocarpa (AML-M), A. × prunifolia (AP-M), which have not been planted yet in the arboretum of Main Botanical Garden of the Russian Academy of Sciences (Moscow). Studying samples were collected in their secondary distribution range. Ethanolic extracts were determined for antioxidant capacity (antioxidant activity by DPPH and phosphomolybdenum methods, the total content of polyphenols, flavonoids, phenolic acids) and measured spectrophotometrically. As standards were used Trolox (TE) for antioxidant activities, gallic acid (GAE) for polyphenol content, quercetin (QE) for flavonoid content, caffeic acid (CAE) for phenolic acid content. The antioxidant activity by DPPH method in ethanol extracts of investigated plants was from 6.96 (AM-D) to 8.89 (AM-OZ) mg TE.g-1 DW. Reducing the power of investigated extracts exhibited activity from 151.47 (AM-OZ) to 297.8 (AA-M) mg TE.g-1 DW. The content of polyphenol compounds determined from 25.98 (AM-TCH) to 54.39 (AA-M) mg GAE.g-1 DW, phenolic acids content was from 7.76 (AP-M) to 11.87 (AM-D) mg CAE.g-1 DW and the content of flavonoids detected from 8.12 (AM-OZ) to 16.62 (AM-D) mg QE.-1 DW. Obtained data showed a strong correlation between the content of polyphenol compounds and reducing the power of extracts (r = 0.700), between flavonoids and phenolic acids (r = 0.771) and also between phenolic acids and reducing power (r = 0.753) in Aronia ethanol extracts. Fruits of investigated species of Aronia can be propagated as a source of polyphenol compounds with antioxidant activity and obtained results may use for farther pharmacological study.

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Comparison of the Antioxidant Activities and Phenolic Content of Five Lonicera Flowers by HPLC-DAD/MS-DPPH and Chemometrics

International Journal of Analytical Chemistry ◽

10.1155/2020/2348903 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Rong-rong Zhou ◽

Xue-hui Liu ◽

Lin Chen ◽

Jian-hua Huang ◽

Xue-juan Liang ◽

...

Keyword(s):

Principal Component Analysis ◽

Antioxidant Activity ◽

Phenolic Content ◽

Antioxidant Activities ◽

Principal Component ◽

Potential Health ◽

Caffeoylquinic Acid ◽

Acid Methyl ◽

Dpph Method ◽

Comprehensive Development

The Lonicera plants (family Caprifoliaceae) with strong antioxidant activity are used as potential health-supporting phytochemicals. Studying the detailed relationships between bioactive compounds and their antioxidant activity is important for further comprehensive development and application of them. In this paper, the antioxidant capacities and compositions of five species of Lonicera flowers were investigated by using the online HPLC-DAD/MS-DPPH method. Results indicated that the samples contained higher amounts of phenols had better antioxidant activity. Furthermore, principal component analysis and linear regression were further used to analyze the correlations between antioxidant capacity and compounds and find the compounds having higher contribution to antioxidant activity. 5-O-Caffeoylquinic acid, 4-O-caffeoylshikimic acid, methyl-5-O-caffeoylquinate, 1,4-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid were screened as stronger antioxidant candidates. In this study, HPLC-DAD/MS and antioxidant activity methods were combined together to analyze the compounds’ information and activity assays of Lonicera, which might provide more evidence for its quality control.

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Correlation of Total Phenolic, Flavonoid and Carotenoid Content of Sesbania sesban (L. Merr) Leaves Extract with DPPH Scavenging Activties

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/ijpapr.v9i1.8046 ◽

2017 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Sani N. Fitriansyah ◽

Irda Fidrianny ◽

Komar Ruslan

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Activities ◽

Carotenoid Content ◽

Total Phenolic ◽

Sesbania Sesban ◽

Total Carotenoid Content ◽

Dpph Method ◽

Dpph Scavenging

Antioxidants are found in many plants and can neutralize free radicals. Sesbania sesban (L.)Merr is a plant that has been used empirically by Indonesian people and its proven have many pharmacologycal activity. The objectives of this research were to study antioxidant activity Sesbania sesban leaves extract using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and correlation with its total phenolic, flavonoid and carotenoid content. Extraction was performed by reflux using different polarity solvent. The extracts were vaporated using rotary evaporator. Antioxidant activity was tested using DPPH assay. Determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with inhibitory concentration 50 (IC50) DPPH scavenging activity were analyzed by Pearson’s method. Nhexane, ethyl acetate and ethanol extracts of Sesbania sesban leaves had IC50 DPPH  50 g/ml and it can be classified as very strong antioxidant. Ethanol extract of Sesbania sesban had the highest of total phenolic content (5.18 g GAE/100 g) and highest total flavonoid content (4.56 g QE/100 g), while the highest total carotenoid content (4.56 g BE/100 g) was given by n-hexane extract. Total phenolic content in Sesbania sesban leaves extracts had significant and negative correlation with their IC50 DPPH scavenging activities. Phenolic compounds in Sesbania sesban leaves extracts were contributor major in its antioxidant activities by DPPH method.

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Endophytic Fungi of Brunfelsia Uniflora: Isolation, Cryopreservation, and Determination of Enzymatic and Antioxidant Activity

10.21203/rs.3.rs-734603/v1 ◽

2021 ◽

Author(s):

Sara Jane Marsola ◽

Lais Freitas Jorge ◽

Adriano Broges Menequeti ◽

Miria Benetati Delgado Berteli ◽

Thaís Emanuelle Feijó Lima ◽

...

Keyword(s):

Antioxidant Activity ◽

Endophytic Fungi ◽

Laccase Activity ◽

Antioxidant Activities ◽

Sucrose Solution ◽

Biological Activities ◽

Cellulase Activity ◽

Total Phenolic ◽

Malt Extract ◽

Phenolic Contents

Abstract Brunfelsia uniflora (Pohl.) D. Don (Solanaceae), commonly known as manacá-de-cheiro , is widely distributed in Brazil and used by local indigenous peoples as an antirheumatic, antisyphilitic , depurative, emetic, vermifuge, and purgative agent. Several studies have examined the biological activities and phytochemical profile of Brunfelsia ; however, few have focused on the diversity of endophytic microorganisms that colonize members of the genus. This study aimed to isolate and cryopreserve endophytic fungi from B. uniflora and determine their cellulase, laccase, and antioxidant activities. Endophytic fungi were isolated from B. uniflora stems, cultured on wheat grains, immersed in a 150 g/L aqueous sucrose solution, and cryopreserved at −80°C for 1 and 6 months. Cellulase activity was determined by a qualitative test using carboxymethylcellulose medium and laccase activity by a quantitative test based on the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate). Prior to antioxidant activity assays, fungi were grown in malt extract broth for production of mycelial biomass. A methanolic extract was prepared for evaluation of DPPH• scavenging activity, FRAP activity, and total phenolic content. A total of 46 endophytic fungal isolates were obtained from B. uniflora stems and classified into 24 groups according to morphological similarities. B. uniflora was shown to harbor a great diversity of endophytic fungi . Mycelial viability was observed after 1 and 6 months of cryopreservation at −80 °C. Fungi exhibited cellulase and laccase activities. Isolate CE23 had the highest laccase activity after 7 days of cultivation. Twelve isolates were found to have low total phenolic contents and DPPH• and FRAP activities.

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