scholarly journals Effect of Muntingia calabura L. Stem Bark Extracts on Uric Acid Concentration and Renal Histopathology in Diabetic Rats

Medicina ◽  
2019 ◽  
Vol 55 (10) ◽  
pp. 695
Author(s):  
Safrida Safrida ◽  
Mustafa Sabri
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Acid Concentration ◽  
Stem Bark ◽  
Diabetic Rats ◽  
Bark Extract ◽  
Uric Acid Concentration ◽  
Renal Histopathology ◽  
Stem Bark Extract ◽  
Muntingia Calabura

Background and objectives: This study was designed to determine uric acid concentration and renal histopathology of Muntingia calabura L. stem bark extract in diabetic rats and to compare the natural product of M. calabura L. stem bark extract with allopurinol. Materials and Methods: A completely randomized design was used for the experiment, which consisted of six treatment groups, each consisting of four rats, as follows: 1) NR, normal rat; 2) KN, diabetic rat (negative control); 3) KP, diabetic rats given allopurinol 10 mg/kg body weight; 4) EM150, diabetic rats given the test extract 150 mg/kg body weight/day; 5) EM300, diabetic rats given the test extract 300 mg/kg body weight/day; and 6) EM450, diabetic rats given for extract 450 mg/kg body weight/ day. Results: The results showed that M. calabura L. stem bark extract decreased (p < 0.05) uric acid concentrations in diabetic rats and no specific damage to renal proximal tubular cells was seen. Conclusions: It was concluded that M. calabura L. stem bark extract has a potential as an antihyperuricemic in diabetic rats. The recommended dose was 300 mg/kg body weight to provide a significant effect on reducing the uric acid level in diabetic rats. Our results support the use of this plant for the treatment of degenerative and inflammatory diseases.

scholarly journals Effect of Muntingia Calabura L. Stem Bark Extracts on Uric Acid Concentration and Renal Histopathology in Diabetic Rats

2019 ◽  
Author(s):  
Safrida Safrida ◽  
Mustafa Sabri
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Acid Concentration ◽  
Stem Bark ◽  
Diabetic Rats ◽  
Bark Extract ◽  
Diabetic Rat ◽  
Uric Acid Concentration ◽  
Renal Histopathology ◽  
Stem Bark Extract

Background and objectives: This study were designed to determine uric acid concentration and renal histopathology of M. calabura L. stem bark extract in diabetic rats and to compare the natural product of M. calabura L. stem bark extract with allopurinol. Materials and Methods: A completely randomized design was used for the experiment which consisted of 6 treatment groups, each consisting of 4 rats, as follows: 1) NR, normal rat; 2) KN, diabetic rat (negative control); 3) KP, diabetic rats given allopurinol 10 mg/kg body weight; 4) EM150, diabetic rats given the test extract 150 mg/kg body weight/day; 5) EM300, diabetic rats given the test extract 300 mg/kg body weight/day; and 6) EM450, diabetic rats given the test extract 450 mg/kg body weight/ day. Results: The results showed that M. calabura L. stem bark extract decrease (p&lt;0.05) uric acid levels in diabetic rats and no specific damage to renal proximal tubular cells was seen. Conclusions: It was concluded that M. calabura L. stem bark extract has a potential as an antihyperuricemic in diabetic rats. The recommended does was 300 mg/kg body weight to provide a significant effect on reducing the uric acid level in diabetic rats. Our findings support the use of this plant as a treatment for gout and other inflammatory diseases.

scholarly journals Effect of Carica papaya L. Stem Bark Extracts on Cholesterol Concentration in Rats Induced with Streptosozin

2020 ◽  
Vol 151 ◽  
pp. 01011
Author(s):  
Safrida Safrida ◽  
Mustafa Sabri
Keyword(s):  
Body Weight ◽  
Carica Papaya ◽  
Stem Bark ◽  
Negative Control ◽  
Diabetic Rats ◽  
Cholesterol Concentration ◽  
Bark Extract ◽  
Treatment Groups ◽  
Randomized Design ◽  
Stem Bark Extract

This study was designed to determine the effect of Carica papaya L. stem bark extracts on cholesterol concentration in rats induced with glibenclamide. A completely randomized design was used for the experiment which consisted of 6 treatment groups, each group consisted of four rats, as follows:1) KN (negative control, non-diabetic rats); KP, diabetic rats given glibenclamide 10 mg/kg body weight; EP 1, diabetic rats given 0 mg/kg body weight/day extract; EP2, diabetic rats given 100 mg/kg body weight/day extract; and EP3, diabetic rats given 200 mg/kg body weight/day extract, EP4, diabetic rats given 300 mg/kg body weight/day extract for 28 day. The results showed that C. papaya L. stem bark extract decreased (P<0.05) cholesterol levels in diabetic rats. It was concluded that C. papaya L. stem bark extract had potential as anti-hypercholesterolemic in diabetic rats.

Anti-Hyperglycemic and Anti-Hyperlipidemic Effects of Aqueous Stem Bark Extract of Acacia albida Delile. in Alloxan-Induced Diabetic Rats

2014 ◽  
Vol 9 (4) ◽  
pp. 170-178 ◽  
Author(s):  
I.A. Umar ◽  
Aminu Mohammed ◽  
U.S. Ndidi ◽  
A.B. Abdulazeez ◽  
W.C. Olisa ◽  
...  
Keyword(s):  
Stem Bark ◽  
Diabetic Rats ◽  
Bark Extract ◽  
Acacia Albida ◽  
Stem Bark Extract

scholarly journals Biochemical and Histopathological Effect of Detarium microcarpum Stem Bark Extract in Wistar Albino Rats

2020 ◽  
pp. 1-9
Author(s):  
Abubakar Bilyamini Mu’azu ◽  
Yusif Bello Baba ◽  
Adamu Idris Matinja
Keyword(s):  
Body Weight ◽  
Lethal Dose ◽  
Stem Bark ◽  
Bark Extract ◽  
Albino Rats ◽  
Cardiac Muscle Cells ◽  
Hepatocellular Necrosis ◽  
Detarium Microcarpum ◽  
Stem Bark Extract ◽  
Histopathological Studies

Aim: In this study, the methanol stem bark extract of Detarium microcarpum was evaluated for sub-chronic, biochemical and histopathological studies. Methodology: Sub-chronic toxicity studies was investigated in rats administered with 35, 70 and 140 mg/kg doses of the extract orally for 28 days using standard laboratory procedures after the acute toxicity was carried out. Results: The median lethal dose (LD50) of the extract was calculated to be equal to (≥) 5000 mg/kg body weight in rats orally. Serological studies revealed significant (p<0.05) decrease in Alanine aminotransferase (ALT) at all doses tested, while at 140 mg/kg it caused a significant (p<0.05) increase in Alkaline Phosphatase (ALP). At doses of 70 and 140 mg/kg there was a significant (p<0.05) reduction in creatinine level. Histopathological studies on the liver showed moderate hepatocellular necrosis at doses of 35 and 70 mg/kg, while at 140 mg/kg there was intense hepatocellular necrosis,  Kupffer cells and lymphocytes hyperplasia. The Kidney showed intense necrosis of tubules and glomerular necrosis with lymphocytes hyperplasia at all doses tested.  The spleen also showed intense lymphocyte hyperplasia at all doses with sinusoidal congestion at the lowest dose of 35 mg/kg. The heart showed slight necrosis of cardiac muscle cells at all doses with blood congestion at 35 and 70mg/kg body weight. Conclusion: The study indicates that prolong use of the extract in the management of disease conditions may be associated with some adverse effect of some vital organs.

scholarly journals Partial elucidation on the remedial effect of stem-bark extract of Moringa oleifera on diabetic rats

2021 ◽  
Vol 015 (02) ◽  
pp. 111-116
Author(s):  
Chukwuedozie Francis Nwachukwu
Keyword(s):  
Body Weight ◽  
Enzyme Activities ◽  
Moringa Oleifera ◽  
Glucose Utilization ◽  
Stem Bark ◽  
Vehicle Group ◽  
Bark Extract ◽  
Significant Difference ◽  
Stem Bark Extract ◽  
Group 2

Diabetes is growing public health. The research investigated the modulatory roles of the aqueous stem-bark extract of Moringa oleifera on glucose utilization. A modified Oral Glucose Tolerance Test (OGTT) was used in studying the effect of the extract on glucose absorption, on four groups of six rats and standard methods were used to test the effect of the extract on enzyme activities (hexokinase and glucose 6 phosphatase) on three groups of six rats. In OGTT, rats in group-1(diabetic control) and group-4 (normal control) were administered with the vehicle only. The other groups were administered different concentrations of the extract in the vehicle (group-2 and 3 were 200mg/kg body weight and 300mg/kg respectively). In the enzymes activities, 200mg/kg body weight of the extract was administered to diabetic treated group whereas normal untreated and diabetic untreated received 5ml of water only. Glucose concentrations of OGTT showed increased concentration in the first 30 minutes after administration of the extract and steady time-dependent decreased concentration through 120 minutes. Group-3, showed a significant difference in each 30 minutes interval, compared to the 120 minutes (p<0.05). Each interval is significantly different from the preceded 30 minutes interval (p<0.005). Group-2 was significantly different in the first and second 30-minutes intervals, compared to the preceded interval (p<0.005). The first 30 minute interval was significantly different from the baseline and 120 minutes (p<0.05). In enzyme activities, the diabetic treated and normal untreated were significantly different from the diabetic untreated (p<0.05). The extract improved glucose utilization.

scholarly journals Anti-hyperglycemic and Antioxidant Potential of Water-Ethanol Extract of Musanga cecropioides Stem Bark

2016 ◽  
Vol 8 (01) ◽  
Author(s):  
Nyemb Nyunaï ◽  
Abel Joël Yaya ◽  
Thierry Gilbert Tabi ◽  
Armelle Tchamgoue ◽  
Marie Ngondé ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Diabetic Rats ◽  
Total Flavonoid ◽  
Bark Extract ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Stem Bark Extract

The study investigated the effect of Musanga cecropioides (MC) water-ethanol stem bark extract on blood glucose level in both hyperglycemic loaded glucose rats and streptozotocin (STZ)-induced diabetic rats, and evaluated its antioxidant capacity. The Wistar rats were induced diabetes after fasting. Oral Glucose Tolerance Test (OGTT) was conducted on normoglycemic rats, and anti-hyperglycemic test on diabetic rats; five groups with five rats each were constituted. Group 1: negative control was treated with vehicle; Group 2, Group 3, and Group 4 were treated with increasing water-ethanol extract (200, 300 and 400 mg/kg b.w); Group 5 was the positive control, treated with glibenclamide. The antioxidant capacity of the extract was also evaluated by measuring the Ferric Reducing Antioxidant Power, Total Phenolic Content, Total Flavonoid Content, and radical scavenging activity of water-ethanol stem bark extract. In OGTT the water-ethanol extract of MC, at the dose of 300 mg/kg, significantly lowered the Area under Curve (AUC) induced by glucose. In STZ diabetic rats, the extract significantly lowered the AUC of blood glucose, at all doses. Glibenclamide was more efficient in both OGTT and anti-hyperglycemic test. The MC extract presented relevant antioxidant activity with IC50 = 6.23 mg/mL. Both the Total Phenolic Content and the Total Flavonoid Content increased in a dose-dependent manner. The correlation of DPPH % free radical scavenged and Total Flavonoid Content was positive and statistically significant. MC water-ethanol extract possesses a good antioxidant potential, and could be helpful to lower hyperglycemic state associated with diabetes.

scholarly journals 28 Days Sub Acute Toxicity Studies of the Methanol Stem Bark Extract of Combretum hypopilinum Diels (Combretacea) in Rats

2021 ◽  
Vol 6 (3) ◽  
pp. 65-73
Author(s):  
Abubakar Kabiru ◽  
Usman Aminu ◽  
Yerima Musa ◽  
Muhammad Abubakar Amali ◽  
Samaila Hassan ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Oral Administration ◽  
Histopathological Examination ◽  
Toxicity Testing ◽  
Stem Bark ◽  
Bark Extract ◽  
Normal Ranges ◽  
Stem Bark Extract ◽  
Oral Acute Toxicity

The aim of this study was to evaluate the safety profile of Combretum hypopilinum stem bark extracted with Methanol (70%v/v). Preliminary Phytochemical screening of the crude methanol stem bark extract was carried out, and revealed the presence of secondary metabolites such as steroids, flavonoids and alkaloids. Initial oral acute toxicity test was carried out using the Limit Dose Test to ascertain the safety of the extract in rats. Sub-acute toxicity testing was conducted by 28 days oral administration of 400 mg/kg, 800 mg/kg and 1600 mg/kg body weight to three groups of ten rats. The fourth group was administered distilled water 10 ml/kg. No major changes were observed in body weight of the animals following 28 days of daily oral administration. Biochemical parameters such as Total Protein, Total Bilirubin, Creatinine, Aspartate Transaminase (AST) and Alanine Transaminase (ALT), were found to be within normal ranges. The levels of marker enzymes in the vital organs did not show any significant changes between control and treated groups. Histopathological examination of the major vital organs (liver, brain, and kidney) revealed no significant pathological changes in the treated groups of rats. The results of the present work suggested that the methanol stem bark extract of Combretum hypopilinum is relatively safe for use at the tested doses.

Sign in / Sign up

Export Citation Format

Share Document