scholarly journals Prediction of Autoimmune Diseases by Targeted Metabolomic Assay of Urinary Organic Acids

Metabolites ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 502
Author(s):  
Dimitris Tsoukalas ◽  
Vassileios Fragoulakis ◽  
Evangelos Papakonstantinou ◽  
Maria Antonaki ◽  
Athanassios Vozikis ◽  
...  
Keyword(s):  
Autoimmune Diseases ◽  
Organic Acids ◽  
Predictive Accuracy ◽  
Binary Logistic Regression ◽  
Gas Chromatography Mass Spectrometry ◽  
Diagnostic Tools ◽  
Loss Of Self ◽  
Urine Organic Acid ◽  
Urine Organic Acids ◽  
Glutathione Cycle

Autoimmune diseases (ADs) are chronic disorders characterized by the loss of self-tolerance, and although being heterogeneous, they share common pathogenic mechanisms. Self-antigens and inflammation markers are established diagnostic tools; however, the metabolic imbalances that underlie ADs are poorly described. The study aimed to employ metabolomics for the detection of disease-related changes in autoimmune diseases that could have predictive value. Quantitative analysis of 28 urine organic acids was performed using Gas Chromatography-Mass Spectrometry in a group of 392 participants. Autoimmune thyroiditis, inflammatory bowel disease, psoriasis and rheumatoid arthritis were the most prevalent autoimmune diseases of the study. Statistically significant differences were observed in the tricarboxylate cycle metabolites, succinate, methylcitrate and malate, the pyroglutamate and 2-hydroxybutyrate from the glutathione cycle and the metabolites methylmalonate, 4-hydroxyphenylpyruvate, 2-hydroxyglutarate and 2-hydroxyisobutyrate between the AD group and the control. Artificial neural networks and Binary logistic regression resulted in the highest predictive accuracy scores (66.7% and 74.9%, respectively), while Methylmalonate, 2-Hydroxyglutarate and 2-hydroxybutyrate were proposed as potential biomarkers for autoimmune diseases. Urine organic acid levels related to the mechanisms of energy production and detoxification were associated with the presence of autoimmune diseases and could be an adjunct tool for early diagnosis and prediction.

scholarly journals COMPARATIVE GAS CHROMATOGRAPHIC (GC) ANALYSIS OF SERUM AND URINE ORGANIC ACIDS IN HEALTHY TERM INFANTS

1974 ◽  
Vol 8 (4) ◽  
pp. 392-392
Author(s):  
Serge B Melançon ◽  
Bernard Grignon ◽  
Michel Potier ◽  
Louis Dallaire
Keyword(s):  
Organic Acids ◽  
Term Infants ◽  
Gc Analysis ◽  
Urine Organic Acids ◽  
Serum And Urine

scholarly journals Analysis of chemical characteristics of lignite upgrading wastewater and its agricultural utilization

2020 ◽  
Vol 81 (7) ◽  
pp. 1530-1540
Author(s):  
Huifen Zhang ◽  
Ji Song ◽  
Mi Zhang ◽  
Cheng Xiang ◽  
Baocai Li ◽  
...  
Keyword(s):  
Organic Acids ◽  
Quality Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Agricultural Field ◽  
Wheat Seedlings ◽  
Liquid Fertilizer ◽  
Material Basis ◽  
Physical Form ◽  
Polluted Waters ◽  
Very High

Abstract The lignite upgrading wastewater (LUW) produced in the drying and upgrading process of lignite cannot be discharged directly. Conventional wastewater treatment methods are usually costly and unable to achieve efficient utilization of water resources which are rich in activity components. In this study, the water quality analysis showed that LUW belonged to seriously polluted waters with low pH and very high total nitrogen content. Fifty-five compounds, mainly phenols and organic acids, were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The study confirmed that the LUW, after being diluted to an appropriate concentration, could significantly promote the growth of wheat seedlings. The phenols and organic acids were the activity material basis of LUW, which promoted seed germination possibly through playing a role similar to plant hormones and simultaneously enhancing the utilization of nutrient elements. LUW had the natural advantages of directly developing high-end liquid fertilizers in terms of its physical form, chemical composition, biological activity, safety and economy. This study confirmed the feasibility of applying LUW to agricultural field as liquid fertilizer only through simple dilution without other treatments. Applying LUW as liquid fertilizer can not only supply a fertilizer product with low production cost and outstanding efficacy, but also provide an efficient and green way for the treatment of upgrading wastewater, which utilize the LUW as natural resources instead of purifying and discharging.

scholarly journals Evaluation of Physiological Characteristics, Soluble Sugars, Organic Acids and Volatile Compounds in ‘Orin’ Apples (Malus domestica) at Different Ripening Stages

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 807
Author(s):  
Shunbo Yang ◽  
Zhipeng Meng ◽  
Yanan Li ◽  
Rongxin Chen ◽  
Yazhou Yang ◽  
...  
Keyword(s):  
Organic Acids ◽  
Volatile Compounds ◽  
Solid Phase ◽  
Soluble Sugars ◽  
Titratable Acidity ◽  
Fruit Weight ◽  
Physiological Characteristics ◽  
Gas Chromatography Mass Spectrometry ◽  
Apple Cultivar ◽  
Ripening Stages

‘Orin’ is a popular apple cultivar, which has a yellow-green appearance, pleasant taste, and unique aroma. However, few studies on the fruit quality characteristics of ‘Orin’ apples have been reported before. In this study, changes of the physiological characteristics were measured at different ripening stages, and the soluble sugars and organic acids were determined by high-performance liquid chromatography (HPLC). Volatile compounds were identified using the headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). During the fruit ripening, the ‘Orin’ apple fruit weight, size, and total soluble solid were gradually increased by contrast with the titratable acidity, and the firmness decreased. The content of four soluble sugars reached the maximum at the 180 days after full bloom (DAFB) stage. Malic acid was measured as the most abundant organic acid in ‘Orin’ apples. Ethyl butyrate, hexyl propanoate, hexyl acetate and butyl acetate belonging to esters with high odor activity values (OAVs) could be responsible for the typical aroma of ‘Orin’ apples. The aim of this work was to provide information on the flavor characteristics of ‘Orin’ apples and promote this apple cultivar for marketing and processing in the future.

The Diagnostic Value of Digital Subtraction Arthrography and Radionuclide Arthrography in Revision Total Hip Arthroplasty / Der diagnostische Wert der digitalen Subtraktionsarthrographie und der Radionuklidarthrographie in der Revisionschirurgie des Hüftgelenkersatzes

2004 ◽  
Vol 49 (12) ◽  
Author(s):  
Marius von Knoch ◽  
Bertram Barden ◽  
Guido Saxler ◽  
Franz Löer
Keyword(s):  
Total Hip Arthroplasty ◽  
Hip Arthroplasty ◽  
Predictive Accuracy ◽  
Diagnostic Value ◽  
Diagnostic Tools ◽  
Digital Subtraction ◽  
Plain Radiographs ◽  
Total Hip ◽  
The Status ◽  
Sensitivity Specificity

AbstractAn analysis of plain radiographs, digital subtraction arthrography, and radionuclide arthrography was performed in 25 revision hip arthroplasties to evaluate the efficacy and usefulness of these methods in the diagnosis of loosening. The findings by each method were compared with intraoperative assessment of the status of components and expressed in terms of sensitivity, specificity, and predictive accuracy. Overall accuracy for the acetabular component by plain radiographs was 80%; by digital subtraction arthrography, 88%; by radionuclide arthrography, 68%. Overall accuracy for the femoral component by plain radiographs was 92%; by digital subtraction arthrography, 84%; radionuclide arthrography, 76%. We consider subtraction arthrography and radionuclide arthrography to be adjuvant diagnostic tools which may be indicated in individual cases of suspected implant loosening of total hip arthroplasty. The routine use of these two methods is not warranted when compared to plain radiographs. In 25 Fällen von Revisionsoperationen von Hüftgelenksersatz wurden Röntgenbilder, digitale Subtraktionsarthrographien und Radionuklidarthrographien analysiert, um die Effizienz dieser Methoden hinsichtlich der Diagnose von Implantatlockerungen festzustellen. Die Ergebnisse der drei genannten radiologischen Untersuchungsmethoden wurden mit der intraoperativ festgestellten Implantatstabilität verglichen. Es wurde die Sensitivität, Spezifität und die Vorhersagegenauigkeit für alle drei Methoden bestimmt. Die Vorhersagegenauigkeit für die acetabulären Komponenten betrug 80% für Röntgenbilder, 88% für digitale Subtraktionsarthrographien und 68% für Radionuklidarthrographien. Die Vorhersagegenauigkeit für die femoralen Komponenten betrug 92% für Röntgenbilder, 84% für digitale Subtraktionsarthrographien und 76% für Radionuklidarthrographien.Wir sehen in der Subtraktionsarthrographie und der Radionuklidarthrographie adjuvante diagnostische Mittel, welche im Einzelfall bei fraglich gelockerten Implantaten indiziert sind. Als Routinemassnahme scheinen diese beiden Methoden im Vergleich zu einfachen Röntgenbildern aber nicht indiziert zu sein.

scholarly journals The Histone Modification Code in the Pathogenesis of Autoimmune Diseases

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Yasuto Araki ◽  
Toshihide Mimura
Keyword(s):  
Autoimmune Diseases ◽  
Histone Modifications ◽  
Lupus Erythematosus ◽  
Cell Types ◽  
Biliary Cirrhosis ◽  
Loss Of Self ◽  
Self Tolerance ◽  
Different Cell Types

Autoimmune diseases are chronic inflammatory disorders caused by a loss of self-tolerance, which is characterized by the appearance of autoantibodies and/or autoreactive lymphocytes and the impaired suppressive function of regulatory T cells. The pathogenesis of autoimmune diseases is extremely complex and remains largely unknown. Recent advances indicate that environmental factors trigger autoimmune diseases in genetically predisposed individuals. In addition, accumulating results have indicated a potential role of epigenetic mechanisms, such as histone modifications, in the development of autoimmune diseases. Histone modifications regulate the chromatin states and gene transcription without any change in the DNA sequence, possibly resulting in phenotype alteration in several different cell types. In this paper, we discuss the significant roles of histone modifications involved in the pathogenesis of autoimmune diseases, including rheumatoid arthritis, systemic lupus erythematosus, systemic sclerosis, primary biliary cirrhosis, and type 1 diabetes.

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