scholarly journals 1H-Nuclear Magnetic Resonance Analysis of Urine as Diagnostic Tool for Organic Acidemias and Aminoacidopathies

Metabolites ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 891
Author(s):  
Ninna Pulido ◽  
Johana M. Guevara-Morales ◽  
Alexander Rodriguez-López ◽  
Álvaro Pulido ◽  
Jhon Díaz ◽  
...  
Keyword(s):  
1H Nmr ◽  
Nuclear Magnetic Resonance Analysis ◽  
Type I ◽  
Inborn Errors ◽  
Resonance Analysis ◽  
Organic Acidemias ◽  
Metabolite Profiles ◽  
1H Nuclear Magnetic Resonance ◽  
Chain Acyl ◽  
Spectral Profiles

The utility of low-resolution 1H-NMR analysis for the identification of biomarkers provided evidence for rapid biochemical diagnoses of organic acidemia and aminoacidopathy. 1H-NMR, with a sensitivity expected for a field strength of 400 MHz at 64 scans was used to establish the metabolomic urine sample profiles of an infant population diagnosed with small molecule Inborn Errors of Metabolism (smIEM) compared to unaffected individuals. A qualitative differentiation of the 1H-NMR spectral profiles of urine samples obtained from individuals affected by different organic acidemias and aminoacidopathies was achieved in combination with GC–MS. The smIEM disorders investigated in this study included phenylalanine metabolism; isovaleric, propionic, 3-methylglutaconicm and glutaric type I acidemia; and deficiencies in medium chain acyl-coenzyme and holocarboxylase synthase. The observed metabolites were comparable and similar to those reported in the literature, as well as to those detected with higher-resolution NMR. In this study, diagnostic marker metabolites were identified for the smIEM disorders. In some cases, changes in metabolite profiles differentiated post-treatments and follow-ups while allowing for the establishment of different clinical states of a biochemical disorder. In addition, for the first time, a 1H-NMR-based biomarker profile was established for holocarboxylase synthase deficiency spectrum.

scholarly journals Nitric oxide accelerates germination via the regulation of respiration in chickpea

2019 ◽  
Vol 70 (17) ◽  
pp. 4539-4555 ◽  
Author(s):  
Sonika Pandey ◽  
Aprajita Kumari ◽  
Manu Shree ◽  
Vinod Kumar ◽  
Pooja Singh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Pentose Phosphate ◽  
Regulation Of Respiration ◽  
Nuclear Magnetic Resonance Analysis ◽  
No Donor ◽  
Resonance Analysis ◽  
Ros Accumulation ◽  
1H Nuclear Magnetic Resonance ◽  
Plant Life Cycle

Abstract Seed germination is crucial for the plant life cycle. We investigated the role of nitric oxide (NO) in two chickpea varieties that differ in germination capacity: Kabuli, which has a low rate of germination and germinates slowly, and Desi, which shows improved germination properties. Desi produced more NO than Kabuli and had lower respiratory rates. As a result of the high respiration rates, Kabuli had higher levels of reactive oxygen species (ROS). Treatment with the NO donor S-nitroso-N-acetyl-D,L-penicillamine (SNAP) reduced respiration in Kabuli and decreased ROS levels, resulting in accelerated germination rates. These findings suggest that NO plays a key role in the germination of Kabuli. SNAP increased the levels of transcripts encoding enzymes involved in carbohydrate metabolism and the cell cycle. Moreover, the levels of amino acids and organic acids were increased in Kabuli as a result of SNAP treatment. 1H-nuclear magnetic resonance analysis revealed that Kabuli has a higher capacity for glucose oxidation than Desi. An observed SNAP-induced increase in 13C incorporation into soluble alanine may result from enhanced oxidation of exogenous [13C]glucose via glycolysis and the pentose phosphate pathway. A homozygous hybrid that originated from a recombinant inbred line population of a cross between Desi and Kabuli germinated faster and had increased NO levels and a reduced accumulation of ROS compared with Kabuli. Taken together, these findings demonstrate the importance of NO in chickpea germination via the control of respiration and ROS accumulation.

Detection of inborn errors of metabolism using GC-MS: over 3 years of experience in southern China

2015 ◽  
Vol 28 (3-4) ◽  
Author(s):  
MinYan Jiang ◽  
Li Liu ◽  
HuiFen Mei ◽  
XiuZhen Li ◽  
Jing Cheng ◽  
...  
Keyword(s):  
Amino Acid ◽  
Inborn Errors Of Metabolism ◽  
Dehydrogenase Deficiency ◽  
Southern China ◽  
Glutaric Aciduria Type ◽  
Type I ◽  
Peroxisomal Disorders ◽  
Inborn Errors ◽  
Glutaric Aciduria ◽  
Chain Acyl

AbstractInborn errors of metabolism (IEM) have been detected worldwide using gas chromatography mass spectrometry (GC-MS) since the 1980s, but few related reports exist on the incidence, spectrum, and clinical presentation features of IEM in southern China.From January 2009 to March 2012, 16,075 urine samples were collected from patients who were highly suspected of having IEM in Guangzhou Women and Children’s Medical Center. The specimens were evaluated using GC-MS.We diagnosed 303 cases of IEM by urine GC-MS analysis, including 197 cases with amino acid disorders, 86 cases with organic acidurias (OAs), 10 cases with fatty acid oxidative (FAO) disorders, and 10 cases with peroxisomal disorders. Neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD) was the most common (153 cases), followed by methylmalonic aciduria (48 cases), urea cycle disorders (21 cases), phenylketonuria (20 cases), propionic aciduria (11 cases), X-linked adrenoleukodystrophy (10 cases), multiple carboxylase deficiency (8 cases), glutaric aciduria type I (7 cases), isovaleric aciduria (6 cases), glutaric aciduria type II (4 cases), short-chain acyl-CoA dehydrogenase deficiency (4 cases), 3-hydroxy-3-methylglutaric aciduria (3 cases), maple syrup urine disease (2 cases), very long-chain acyl-CoA dehydrogenase deficiency (1 case), malonic aciduria (1 case), mevalonic aciduria (1 case), Canavan disease (1 case), lysine protein intolerance (1 case), and medium-chain acyl-CoA dehydrogenase deficiency (1 case). The clinical and laboratory features of IEM are neurologic signs, jaundice, metabolic acidosis, ketotic hypoglycemia, and hyperammonemia.In our study, GC-MS provided a diagnostic clue to OAs, amino acid disorders, FAO, and peroxisomal disorders. Urease pretreatment is useful for the diagnosis of NICCD. In southern China, the majority of IEM were amino acid disorders and organic acid disorders. FAO disorders were relatively rare, which we need to investigate further.

scholarly journals 1H-Nuclear Magnetic Resonance Analysis of the Triacylglyceride Composition of Cold-Pressed Oil from Camellia japonica

Molecules ◽  
2012 ◽  
Vol 17 (6) ◽  
pp. 6716-6727 ◽  
Author(s):  
Carmen Salinero ◽  
Xesús Feás ◽  
J. Pedro Mansilla ◽  
Julio A. Seijas ◽  
M. Pilar Vázquez-Tato ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nuclear Magnetic Resonance Analysis ◽  
Camellia Japonica ◽  
Resonance Analysis ◽  
1H Nuclear Magnetic Resonance ◽  
Cold Pressed ◽  
Nuclear Magnetic

scholarly journals Using γ-Ray Polymerization-Induced Assemblies to Synthesize Polydopamine Nanocapsules

Polymers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1754 ◽  
Author(s):  
Wenwen Jiang ◽  
Xinyue Zhang ◽  
Yafei Luan ◽  
Rensheng Wang ◽  
Hanzhou Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Dynamics ◽  
Interfacial Polymerization ◽  
Dynamics Simulation ◽  
Nuclear Magnetic Resonance Analysis ◽  
Resonance Analysis ◽  
Spherical Structure ◽  
1H Nuclear Magnetic Resonance ◽  
Γ Ray ◽  
Model Drug

This work reports a simple and robust strategy for synthesis of polydopamine nanocapsules (PDA NCs). First, polymer assemblies were synthesized by a γ-ray-induced liquid–liquid (H2O–acrylate) interface polymerization strategy, in the absence of any surfactants. 1H nuclear magnetic resonance analysis and molecular dynamics simulation reveal that the generation of polymer assemblies largely depends on the hydrophilicity of acrylate and gravity of the oligomers at the interface. By virtue of the spherical structure and mechanic stability of the polymer assemblies, PDA NCs are next prepared by the interfacial polymerization of dopamine onto the assemblies, followed by the removal of templates by using ethanol. The polydopamine nanocapsules are shown to load and release ciprofloxacin (CIP, a model drug), such that the CIP-loaded PDA NCs are able to inhibit the growth of Escherichia coli.

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