scholarly journals Encapsulated Cell Dynamics in Droplet Microfluidic Devices with Sheath Flow

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 839
Author(s):  
Peter E. Beshay ◽  
Ali M. Ibrahim ◽  
Stefanie S. Jeffrey ◽  
Roger T. Howe ◽  
Yasser H. Anis
Keyword(s):  
Flow Field ◽  
High Speed ◽  
Single Cells ◽  
Microfluidic Channel ◽  
Internal Flow ◽  
Shear Stresses ◽  
Rotational Flow ◽  
Label Free ◽  
Cell Dynamics ◽  
Sheath Flow

In this paper we study the dynamics of single cells encapsulated in water-in-oil emulsions in a microchannel. The flow field of a microfluidic channel is coupled to the internal flow field of a droplet through viscous traction at the interface, resulting in a rotational flow field inside the droplet. An encapsulated single cell being subjected to this flow field responds by undergoing multiple orbits, spins, and deformations that depend on its physical properties. Monitoring the cell dynamics, using a high-speed camera, can lead to the development of new label-free methods for the detection of rare cells, based on their biomechanical properties. A sheath flow microchannel was proposed to strengthen the rotational flow field inside droplets flowing in Poiseuille flow conditions. A numerical model was developed to investigate the effect of various parameters on the rotational flow field inside a droplet. The multi-phase flow model required the tracking of the fluid–fluid interface, which deforms over time due to the applied shear stresses. Experiments confirmed the significant effect of the sheath flow rate on the cell dynamics, where the speed of cell orbiting was doubled. Doubling the cell speed can double the amount of extracted biomechanical information from the encapsulated cell, while it remains within the field of view of the camera used.

scholarly journals Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Soo-Yeon Cho ◽  
Xun Gong ◽  
Volodymyr B. Koman ◽  
Matthias Kuehne ◽  
Sun Jin Moon ◽  
...  
Keyword(s):  
Near Infrared ◽  
Single Cells ◽  
Microfluidic Channel ◽  
Human Monocyte ◽  
Cellular Heterogeneity ◽  
Label Free ◽  
Nanotube Array ◽  
Chemical Cytometry ◽  
Rich Information ◽  
Non Destructive

AbstractNanosensors have proven to be powerful tools to monitor single cells, achieving spatiotemporal precision even at molecular level. However, there has not been way of extending this approach to statistically relevant numbers of living cells. Herein, we design and fabricate nanosensor array in microfluidics that addresses this limitation, creating a Nanosensor Chemical Cytometry (NCC). nIR fluorescent carbon nanotube array is integrated along microfluidic channel through which flowing cells is guided. We can utilize the flowing cell itself as highly informative Gaussian lenses projecting nIR profiles and extract rich information. This unique biophotonic waveguide allows for quantified cross-correlation of biomolecular information with various physical properties and creates label-free chemical cytometer for cellular heterogeneity measurement. As an example, the NCC can profile the immune heterogeneities of human monocyte populations at attomolar sensitivity in completely non-destructive and real-time manner with rate of ~600 cells/hr, highest range demonstrated to date for state-of-the-art chemical cytometry.

scholarly journals Analysis and control of flow at suction connection in high-speed centrifugal pump

2017 ◽  
Vol 9 (1) ◽  
pp. 168781401668529 ◽  
Author(s):  
Wen-wu Song ◽  
Li-chao Wei ◽  
Jie Fu ◽  
Jian-wei Shi ◽  
Xiu-xin Yang ◽  
...  
Keyword(s):  
Flow Field ◽  
Centrifugal Pump ◽  
High Speed ◽  
Internal Flow ◽  
Orifice Plate ◽  
Centrifugal Pumps ◽  
Pressure Area ◽  
Negative Effect ◽  
And Control ◽  
Experimental Data Analysis

The backflow vortexes at the suction connection in high-speed centrifugal pumps have negative effect on the flow field. Setting an orifice plate in front of the inducer is able to decrease the negative effect caused by backflow vortexes. The traditional plate is able to partially control the backflow vortexes, but a small part of the vortex is still in the inlet and the inducer. Four new types of orifice plates were created, and the control effects on backflow vortexes were analyzed. The ANSYS-CFX software was used to numerically simulate a high-speed centrifugal pump. The variations of streamline and velocity vectors at the suction connection were analyzed. Meanwhile, the effects of these plates on the impeller pressure and the internal flow field of the inducer were analyzed. Numerically, simulation and experimental data analysis methods were used to compare the head and efficiency of the high-speed pumps. The results show that the C-type orifice plate can improve the backflow vortex, reduce the low-pressure area, and improve the hydraulic performance of the high-speed pump.

Study on the Turbulent Injury Principle of Blood in the High-Speed Spiral Blood Pump

2011 ◽  
Vol 393-395 ◽  
pp. 992-995
Author(s):  
Zhong Yun ◽  
Chuang Xiang ◽  
Xiao Yan Tang ◽  
Fen Shi
Keyword(s):  
Shear Stress ◽  
Flow Field ◽  
Blood Cell ◽  
Turbulent Flow ◽  
Red Blood Cell ◽  
Blood Cells ◽  
High Speed ◽  
Internal Flow ◽  
Blood Pump ◽  
Turbulent Flow Field

The strongly swirling turbulent flow in the internal flow field of a high-speed spiral blood pump(HSBP), is one of important factors leading to the fragmentation of the red blood cell(RBC) and the hemolysis. The study on the turbulent injure principle of blood in the HSBP is carried out by using the theory of waterpower rotated flow field and the hemorheology. The numerical equation of the strongly swirling turbulent flow field is proposed. The largest stable diameter of red blood cells in the turbulent flow field is analyzed. The determinant gist on the red blood cell turbulent fragmentation is obtained. The results indicate that in the HSMP, when turbulent flow is more powerful, shear stress is weaker, the vortex mass with energy in flow field may cause serious turbulent fragmentation because of the diameter which is smaller than the RBC’s. The RBC’s turbulent breakage will occur when the Weber value is larger than 12.

Detailed Flow Investigations Within a High-Speed Centrifugal Compressor Impeller

1976 ◽  
Vol 98 (3) ◽  
pp. 390-399 ◽  
Author(s):  
D. Eckardt
Keyword(s):  
Flow Field ◽  
Flow Pattern ◽  
High Speed ◽  
Internal Flow ◽  
Suction Side ◽  
Turbulence Structure ◽  
Wake Interaction ◽  
Compressor Impeller ◽  
Channel Curvature ◽  
Relative Flow

Detailed accurate measurements of velocities, directions, and fluctuation intensities were performed with a newly developed laser velocimeter in the internal flow field of a radial discharge impeller, running at tip speeds up to 400 m/s. Relative flow distributions are presented in five measurement areas from inducer inlet to impeller discharge. The impeller flow pattern, which coincides largely with potential-theory calculations in the axial inducer, becomes more and more reversed when the flow separates from the blade suction side, developing a rapidly increasing wake in the radial impeller. The observed secondary flow pattern and effects of channel curvature and system rotation on turbulence structure are discussed with respect to separation onset and jet/wake interaction.

scholarly journals Study of Circulation Drive in High-Speed Rotating Flow Field

2018 ◽  
Vol 186 ◽  
pp. 01001
Author(s):  
Xu Fan ◽  
Bo Ran
Keyword(s):  
Flow Field ◽  
Numerical Computation ◽  
High Speed ◽  
Influence Factors ◽  
Internal Flow ◽  
Rotating Flow ◽  
Factors Affecting ◽  
Cause Of Formation

In the rotor with high speed, there is a certain axial circulation in the internal gas, which is necessary to analyze the cause of formation and influence factors for understanding better the internal flow field. There are many factors affecting the axial circulation. Different circulation drives have different effects on the flow field. In this paper, numerical computation with N-S equations is used to compute the flow field parameters and analyze the mechanism of the flow field. The influence of the temperature of the end cap on the flow field is mainly disscussed. By comparing and analyzing the streamline shape and the size of vortex region under different temperature drive, an effective method is provided for the study of axial circulation in the highspeed rotating flow field.

Numerical Simulation of the Flow in a Large-Scale Thrust Bearing

2010 ◽  
Author(s):  
Hong-Jie Wang ◽  
Ru-Zhi Gong ◽  
De-Ping Lu ◽  
Zhong-De Wu ◽  
Feng-Chen Li
Keyword(s):  
Flow Field ◽  
High Speed ◽  
Large Scale ◽  
Thrust Bearing ◽  
Cfd Simulation ◽  
Internal Flow ◽  
Heat Radiation ◽  
Heavy Load ◽  
Cfd Method ◽  
Water Turbines

Thrust bearing is a key component of large-scale water turbine. It closely relates to the efficiency of large-scale water turbines, and even determines whether the large-scale turbine can operate normally. With the development of the capacitance of water turbines, thrust bearing will develop to the direction of high speed and heavy load. The structure, strength, lubrication and the characteristic of heat radiation of large-scale thrust bearing were often researched in the past. To study the flow condition of the large-scale thrust bearing and analyze the load characteristics, CFD simulation was carried out on the model of thrust bearing. In this study, CFD method was used to simulate the internal flow field of the large-scale thrust bearing. The model researched was a thrust bearing for 1000MW water turbines. The diameter of the thrust bearing was over 5.8 meters, and the maximum thrust load of the bearing can reach to 60MN. The thin gap between the runner and the pad was usually neglected in the published CFD calculations of thrust bearing. But the thin gap was taken into account in this investigation. 1/12 of the model was used as the computational field and periodic boundary was used in the calculation. The standard κ-ε turbulence model was used to simulate the thrust bearing model, and the flow field in the thrust bearing was obtained. The thin gap between the runner and the pad is a wedge. The pressure and velocity distribution in the thrust bearing and thin gap was calculated respectively with conditions of different thin gaps and different rotational speeds of runner. After that, the relationship between carrying capacity and the size of clearance or the speed of the runner through analyzing the data has been obtained from the results of the calculation.

Numerical Analysis of Internal Flow Field in an Impeller with the Time Marching Method

2011 ◽  
Vol 94-96 ◽  
pp. 1476-1480
Author(s):  
Cai Hua Wang
Keyword(s):  
Flow Field ◽  
High Speed ◽  
Pressure Ratio ◽  
Three Dimensional ◽  
Internal Flow ◽  
Centrifugal Impeller ◽  
Three Dimensional Flow ◽  
Vector Distribution ◽  
Time Marching ◽  
Marching Method

Centrifugal compressors are power machineries used widely. Fully understanding of the complex three-dimensional flow field is very important to design higher pressure ratio, higher efficiency centrifugal compressor. In this paper, time marching method is adopted to solve the three-dimensional viscous N-S equations under the relative coordinate system. The internal flow field of the “full controllable vortex” high speed centrifugal impeller is analyzed and the medial velocity vector distribution and the development of the velocity of each section in the impeller are showed. From the figures, it can be seen that the “wake” phenomenon, such as Ecckart described, caused by the curvature, Coriolis force and the boundary layer is exist

scholarly journals ANALYTICAL AND NUMERICAL MODELING METHODS FOR IMPEDANCE ANALYSIS OF SINGLE CELLS ON-CHIP

NANO ◽  
2008 ◽  
Vol 03 (01) ◽  
pp. 55-63 ◽  
Author(s):  
TAO SUN ◽  
NICOLAS G. GREEN ◽  
HYWEL MORGAN
Keyword(s):  
Numerical Modeling ◽  
High Speed ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Equivalent Circuit Model ◽  
Microfluidic Channel ◽  
Electrical Impedance Spectroscopy ◽  
Static Case ◽  
Immobilized Cell ◽  
Modeling Methods

Electrical impedance spectroscopy (EIS) is a noninvasive method for characterizing the dielectric properties of biological particles. The technique can differentiate between cell types and provide information on cell properties through measurement of the permittivity and conductivity of the cell membrane and cytoplasm. In terms of lab-on-a-chip (LOC) technology, cells pass sequentially through the microfluidic channel at high speed and are analyzed individually, rather than as traditionally done on a mixture of particles in suspension. This paper describes the analytical and numerical modeling methods for EIS of single cell analysis in a microfluidic cytometer. The presented modeling methods include Maxwell's mixture theory, equivalent circuit model and finite element method. The difference and advantages of these methods have been discussed. The modeling work has covered the static case — an immobilized cell in suspension and the dynamic case — a moving cell in the channel.

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