scholarly journals Fabrication of Soft Tissue Scaffold-Mimicked Microelectrode Arrays Using Enzyme-Mediated Transfer Printing

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1057
Author(s):  
Yue-Xian Lin ◽  
Shu-Han Li ◽  
Wei-Chen Huang
Keyword(s):  
Microelectrode Arrays ◽  
Covalent Bond ◽  
Biological Properties ◽  
Neural Interface ◽  
Nerve Tissue ◽  
Transfer Printing ◽  
Tissue Scaffold ◽  
One Step ◽  
Structurally Stable ◽  
The Ideal

Hydrogels are the ideal materials in the development of implanted bioactive neural interfaces because of the nerve tissue-mimicked physical and biological properties that can enhance neural interfacing compatibility. However, the integration of hydrogels and rigid/dehydrated electronic microstructure is challenging due to the non-reliable interfacial bonding, whereas hydrogels are not compatible with most conditions required for the micromachined fabrication process. Herein, we propose a new enzyme-mediated transfer printing process to design an adhesive biological hydrogel neural interface. The donor substrate was fabricated via photo-crosslinking of gelatin methacryloyl (GelMA) containing various conductive nanoparticles (NPs), including Ag nanowires (NWs), Pt NWs, and PEDOT:PSS, to form a stretchable conductive bioelectrode, called NP-doped GelMA. On the other hand, a receiver substrate composed of microbial transglutaminase-incorporated gelatin (mTG-Gln) enabled simultaneous temporally controlled gelation and covalent bond-enhanced adhesion to achieve one-step transfer printing of the prefabricated NP-doped GelMA features. The integrated hydrogel microelectrode arrays (MEA) were adhesive, and mechanically/structurally bio-compliant with stable conductivity. The devices were structurally stable in moisture to support the growth of neuronal cells. Despite that the introduction of AgNW and PEDOT:PSS NPs in the hydrogels needed further study to avoid cell toxicity, the PtNW-doped GelMA exhibited a comparable live cell density. This Gln-based MEA is expected to be the next-generation bioactive neural interface.

One-Step Selective Adhesive Transfer Printing for Scalable Fabrication of Stretchable Electronics

2018 ◽  
Vol 3 (3) ◽  
pp. 1700264 ◽  
Author(s):  
Peng Peng ◽  
Kang Wu ◽  
Liangxiong Lv ◽  
Chuan Fei Guo ◽  
Zhigang Wu
Keyword(s):  
Stretchable Electronics ◽  
Transfer Printing ◽  
One Step

scholarly journals A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

2018 ◽  
Vol 9 (41) ◽  
pp. 7981-7985 ◽  
Author(s):  
Yong-Kyun Sim ◽  
Heetae Jung ◽  
Su Hyun Kim ◽  
Jung-Woo Park ◽  
Woo-Jin Park ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Organic Acid ◽  
Covalent Bond ◽  
Step Method ◽  
Weak Organic Acid ◽  
One Step

One-step covalent bond immobilization of biomolecules on silica in water is promoted by weak organic acid using bifunctional linker.

Aqueous chemical grafting of modified-PEG onto maghemite nanoparticles: Influence of grafting conditions

e-Polymers ◽  
2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Claire-Hélène Brachais ◽  
Ling Hu ◽  
Diana Hach ◽  
Denis Chaumont ◽  
Aurélien Percheron ◽  
...  
Keyword(s):  
Covalent Bond ◽  
Nitrogen Adsorption ◽  
Average Diameter ◽  
Size Exclusion ◽  
Maghemite Nanoparticles ◽  
Chemical Grafting ◽  
Exclusion Chromatography ◽  
One Step ◽  
Aqueous Conditions ◽  
Xrd And Tem

AbstractThe synthesis of maghemite nanoparticles coated with triethoxysilanemonomethylether- PEG (Si-mPEG), is presented in aqueous conditions, by the “grafting to” process. This procedure is performed in one step, starting from anchored polymer and native nanoparticles. The maghemite nanoparticles obtained were first identified by XRD and the average diameter is about 10 nm according to the Nitrogen adsorption, XRD and TEM techniques. The nature of the catalysts (acetic acid, triethylamine, NH4OH and dibutyl-tin-dilaurate) used for the establishment of the covalent bond between the γ-Fe2O3 particles and the SimPEG, as well as the influence of the temperature and the reaction time, were evaluated on the grafting rate of the particles in water by TGA and FTIR techniques. Using the size exclusion chromatography, SEC technique, we showed that the monomethoxy-PEG was resistant to mild acidic up to strong basic conditions. The best ratio “time/amount of polymer” for the highest grafting rate was the use of tinbased organometallic compound (DBTL) at 80 °C during 48 h. In these conditions, a grafting rate of 54 % is achieved.

One-step transfer printing of patterned nanogap electrodes

2019 ◽  
Vol 37 (4) ◽  
pp. 040602
Author(s):  
Kai B. Saller ◽  
Hubert Riedl ◽  
Paolo Lugli ◽  
Gregor Koblmüller ◽  
Marc Tornow
Keyword(s):  
Transfer Printing ◽  
One Step ◽  
Nanogap Electrodes

Discussion on Compensation Factor for Large-Radius Bending Die

2013 ◽  
Vol 441 ◽  
pp. 44-47
Author(s):  
Xiao Lei Qian ◽  
Yi Liu ◽  
Rui Guo ◽  
Yuan Qing Zhang ◽  
Guo Qiang Wang
Keyword(s):  
Large Radius ◽  
Automatic Compensation ◽  
Proper Selection ◽  
Die Geometry ◽  
Compensation Factor ◽  
Metal Sheets ◽  
One Step ◽  
Compensation Program ◽  
Selection Of ◽  
The Ideal

For large-radius bending metal sheets with severe springback, an automatic compensation program based on APDL language was developed to achieve the ideal die geometry. To raise compensation efficiency, the range of the ideal compensation factor based on one-step springback analysis was deduced, and tests prove that more iterations will be needed if compensation factor was beyond the range, and proper selection of compensation factor among the range can be conducive to high compensation efficiency.

scholarly journals Current research in development of polycaprolactone filament for 3D bioprinting: a review

2021 ◽  
Vol 926 (1) ◽  
pp. 012080
Author(s):  
C Amni ◽  
Marwan ◽  
S Aprilia ◽  
E Indarti
Keyword(s):  
3D Printing ◽  
Three Dimensional ◽  
Development Stage ◽  
Biological Properties ◽  
Fabrication Process ◽  
3D Bioprinting ◽  
Three Dimensional Printing ◽  
Further Development ◽  
Dimensional Printing ◽  
The Ideal

Abstract Three-dimensional printing (3DP) provides a fast and easy fabrication process without demanding post-processing. 3D-bioprinting is a special class in 3DP. Bio-printing is the process of accurately 3DP structural design using filament. 3D bio-printing technology is still in the development stage, its application in various engineering continues to increase, such as in tissue engineering. As a forming material in 3D printing, many types of commercial filaments have been developed. Filaments can be produced from either natural or synthetic biomaterials alone, or a combination of the two as a hybrid material. The ideal filament must have precise mechanical, rheological and biological properties. Polycaprolactone (PCL) is specifically developed and optimized for bio-printing of 3D structures. PCL is a strategy in 3D printing to better control interconnectivity and porosity spatially. Structural stability and less sensitive properties environmental conditions, such as temperature, humidity, etc make PCL as an ideal material for the FDM fabrication process. In this review, we provide an in-depth discussion of current research on PCL as a filament currently used for 3D bio-printing and outline some future perspectives in their further development.

Sacrificial layer-assisted one-step transfer printing for fabricating a three-layer dry electrode

2020 ◽  
Vol 306 ◽  
pp. 111954
Author(s):  
Junshan Liu ◽  
Yue Zhang ◽  
Han Shan ◽  
Liping Qi ◽  
Hong Tang ◽  
...  
Keyword(s):  
Sacrificial Layer ◽  
Transfer Printing ◽  
Dry Electrode ◽  
One Step

Bodies as behavior

Vitruvian Man ◽  
2019 ◽  
pp. 144-184
Author(s):  
John Oksanish
Keyword(s):  
Bodily Appearance ◽  
One Step ◽  
Bodily State ◽  
Better Than ◽  
The Ideal

The Dinocrates-Alexander episode of book 2 supplies the reader with a complex heuristic for differentiating good architects from bad. Vitruvius claims to rely on his knowledge and writing in anticipation of his own success, whereas he attributes Dinocrates’ renown to an attractive bodily appearance. A close intertextual reading of the passage threaded through Livy 1 suggests that Alexander and Dinocrates violate the ideal architect–autocrat relationship. The manner in which one interprets the episode indicates whether one can distinguish altruism from ambitio and the like. Alexander’s appetitious reaction to Dinocrates and his body is further problematized by the latter’s nudity and evocation of Hercules and athletic victors. Discussions in the rhetorical handbooks indicate that arguments concerning a plaintiff’s or defendant’s bodily state can support arguments about his character. The handbooks seem to presume a widespread valorization of what the Greeks would call καλοκἀγαθία‎, but there is an implicit acknowledgment that beauty dissimulates vice. The athletic and/or gladiatorial body is therefore a particular locus of contestation and controversy, as Cicero’s (and Sallust’s) depictions of Catiline show. On the Greek side, writers as early as Tyrtaeus and Xenophanes had suggested that wisdom is better than strength. Isocrates frames the issue politically, and Vitruvius takes it one step further. Following the Roman handbooks that viewed the cultivation of bodily attributes (vs. the fortuitous possession of those attributes) as the primary signifier of character, Vitruvius suggests that athletes are ethically and politically bankrupt, while writers deserve triumphs and apotheosis. Archimedes, Socrates, and even Vitruvius himself provide counterexamples.

One‐Step Liquid Metal Transfer Printing: Toward Fabrication of Flexible Electronics on Wide Range of Substrates

2018 ◽  
Vol 3 (12) ◽  
pp. 1800265 ◽  
Author(s):  
Rui Guo ◽  
Jianbo Tang ◽  
Shijin Dong ◽  
Ju Lin ◽  
Hongzhang Wang ◽  
...  
Keyword(s):  
Liquid Metal ◽  
Flexible Electronics ◽  
Metal Transfer ◽  
Transfer Printing ◽  
Wide Range ◽  
One Step

Poly(glycerol-sebacate)/poly(caprolactone)/graphene nanocomposites for nerve tissue engineering

2018 ◽  
Vol 33 (5) ◽  
pp. 529-542 ◽  
Author(s):  
Shirin Ghafaralahi ◽  
Mehdi Ebrahimian-Hosseinabadi ◽  
Anousheh Zargar Kharazi
Keyword(s):  
Mechanical Properties ◽  
Cell Attachment ◽  
Weight Ratio ◽  
Sebacic Acid ◽  
Biological Properties ◽  
Attenuated Total Reflection ◽  
Nerve Tissue ◽  
Positive Role ◽  
Nerve Guidance Conduit ◽  
Poly Caprolactone

In this study, mechanical, electrical, physical, and biological properties of polymeric matrixes comprising poly(glycerol-sebacate) (PGS) and poly(caprolactone) (PCL) with various weight ratio of PGS:PCL (1:3 and 1:1) were evaluated in order to apply as nerve guidance conduit. For this purpose, synthetic PGS pre-polymer was acquired using poly-condensation of glycerol and sebacic acid and characterized by attenuated total reflection-fourier transformed infrared (ATR-FTIR) and X-ray diffraction (XRD) spectroscopies. Furthermore, the effect of 1 wt% graphene (Gr) Nano sheets incorporation as filler, was investigated. Blending PGS with PCL significantly improves the hydrophilicity of the samples and improves cells attachment; however, their mechanical properties decreased dramatically. Presence of Gr within the polymeric matrix, significantly increased elastic modulus and tensile strength, which is possibly attributed to its superior mechanical properties and high aspect of ratio. Moreover, aforementioned polymeric matrixes, turned to conductive membranes by addition of Gr, which affected drastically on their biological properties; that way, 3, 4, 5-dimethylthiazol-2, 5-diphenyl tetrazolium bromide assay elucidated that only addition of 1 wt% Gr to the polymeric films resulted in improved cell survival and cell attachment for 7 days of cell seeding. In addition, cell attachment was enhanced considerably by increasing PGS up to 50 wt%, due to positive role of PGS on contact angle reduction. Therefore, the nano-composite film (50PGS-50PCL-1Gr) can be a promising substrate to use as a nerve guidance conduit.

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