scholarly journals Study on the Isolation of Two Atrazine-Degrading Bacteria and the Development of a Microbial Agent

2019 ◽  
Vol 7 (3) ◽  
pp. 80 ◽  
Author(s):  
Jiangwei Zhu ◽  
Li Fu ◽  
Caihua Jin ◽  
Zili Meng ◽  
Ning Yang
Keyword(s):  
Bacillus Licheniformis ◽  
Bacillus Megaterium ◽  
Degradation Efficiency ◽  
High Tolerance ◽  
Degrading Bacteria ◽  
Microbial Agent

Two bacteria capable of efficiently degrading atrazine were isolated from soil, and named ATLJ-5 and ATLJ-11. ATLJ-5 and ATLJ-11 were identified as Bacillus licheniformis and Bacillus megaterium, respectively. The degradation efficiency of atrazine (50 mg/L) by strain ATLJ-5 can reach about 98.6% after 7 days, and strain ATLJ-11 can reach 99.6% under the same conditions. The degradation of atrazine is faster when two strains are used in combination. Adding the proper amount of fresh soil during the degradation of atrazine by these two strains can also increase the degradation efficiency. The strains ATLJ-5 and ATLJ-11 have high tolerance to atrazine, and can tolerate at least 1000 mg/L of atrazine. In addition, the strains ATLJ-5 and ATLJ-11 have been successfully made into a microbial agent that can be used to treat atrazine residues in soil. The degradation efficiency of atrazine (50 mg/kg) could reach 99.0% by this microbial agent after 7 days. These results suggest that the strains ATLJ-5 and ATLJ-11 can be used for the treatment of atrazine pollution.

scholarly journals Aislamiento e identificación de diez cepas bacterianas desnitrificantes a partir de un suelo agrícola contaminado con abonos nitrogenados proveniente de una finca productora de cebolla en la Laguna de Tota, Boyacá, Colombia

Nova ◽  
2006 ◽  
Vol 4 (6) ◽  
pp. 50
Author(s):  
Joaquín L. Benavides López de Mesa ◽  
Gladys M. Quintero ◽  
Olga Lucía Ostos Ortiz
Keyword(s):  
Bacillus Licheniformis ◽  
Bacillus Megaterium ◽  
Pseudomonas Stutzeri ◽  
Acinetobacter Sp ◽  
Corynebacterium Sp

Este articulo presenta los resultados de la versatilidad adaptativa y tolerancia de una consorcio bacteriano constituido por 10 cepas bacterianas endémicas de un suelo agrícola contaminado, proveniente de una finca productora de cebolla ubicada en la laguna de Tota en Aquitania, Boyacá, Colombia, expuesto al uso incontrolado de fertilizantes nitrogenados. La consorcio bacteriano aislado del suelo está conformado por: Bacillus megaterium, Bacillus licheniformis, Pseudomonas stutzeri, Acinetobacter sp, Propionibacterium sp, Peptoestreptococcus sp, Sthaphylococcus coagulasa negativa, Corynebacterium sp, Clostridium sp y Actinomyces sp. Se demostró que los microorganismos aislados tienen capacidad desnitrificante in vitro, transformando el nitrato a nitrógeno molecular. La consorcio bacteriano aislado se constituye en una alternativa de biorremediación para recuperar suelo agrícola contaminado con exceso de fertilizantes nitrogenados.

Isolation and Genetic Identification of Dibenzothiophene Degrading Bacteria from Contaminated Soil

2012 ◽  
Vol 610-613 ◽  
pp. 292-295 ◽  
Author(s):  
Lin Li ◽  
Chao Cheng Zhao ◽  
Qi You Liu ◽  
Yun Bo Zhang
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Contaminated Soil ◽  
Bacterial Strain ◽  
Degradation Efficiency ◽  
Genetic Identification ◽  
Microbial Consortia ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
Degrading Bacteria

The biodegradation abilities of 10 dibenzothiophene degrading microbial consortia isolated from contaminated soil were investigated. 5 highly efficient dibenzothiophene degrading bacterial strains were obtained from the consortium LKY10 by screening on LB-agar plates.The bacterial strain LKY10-5 reduced more than 90% of dibenzothiophene with 40 mg•L-1concentration, and had higher degradation efficiency than enriched bacterial consortia in 7 days of cultivation. According to species identification and phylogenetic analysis, strain LKY10-1 and LKY10-3 belonged to Actinobacteria and could be included in Rhodococcus and Cellulosimicrobium genus, LKY10-5 and LKY10-6 belonged to Proteobacteria and could be included in Pseudomonas and Devosia genus, and LKY10-13 could be included in Lysinibacillus genus and belonged to Firmicutes.

scholarly journals Chlorpyrifos degradation by combined solar photo-Fenton and bacterial metabolism and simultaneous toxicity analysis in zebrafish (Danio rerio)

2022 ◽  
Author(s):  
Tanmaya Nayak ◽  
Arpan Ghosh ◽  
Sourav Das ◽  
Tapan Kumar Adhya ◽  
Paritosh Patel ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Negative Impact ◽  
Degradation Efficiency ◽  
Bacterial Degradation ◽  
Acetylcholinesterase Inhibition ◽  
Environmental Toxicants ◽  
Degrading Bacteria ◽  
Toxicity Analysis ◽  
Control Adult ◽  
Biological Methods

Chlorpyrifos (CP) is a widely used insecticide that has been used extensively, contributing towards a negative impact on public health concerns and associated ecosystems. Bioremediation is one of the key biological methods used for reducing these environmental toxicants. The present study examined the effectiveness of a combined process including solar photo-Fenton process followed by bacterial degradation using Ochrobactrum sp. CPD-03 for effective CP degradation in wastewater. Results showed that solar photo-Fenton treatment had CP degradation efficiency of ~42% in 4 h with a final degradation efficiency of ~92% in 96 h upon combined bacterial degradation. Simultaneous survivability of zebrafish (Danio rerio) was also studied during CP degradation. Compared to control, adult zebrafishes showed increased survivability following the addition of CPD-03 in water resulting a reduced CP concentration. CP toxicity in wastewater had caused acetylcholinesterase inhibition in zebrafish; however, this inhibition is due to absence of CP degrading bacteria. Therefore, a combined approach would influence for regulating CP degradation in wastewater along with simultaneous survival of Danio rerio.

scholarly journals Screening and Characterisation of Keratin-Degrading Bacillus sp. from Feather Waste

2019 ◽  
pp. 1-12
Author(s):  
M. T. Dada ◽  
S. M. Wakil
Keyword(s):  
Bacillus Subtilis ◽  
Proteolytic Activity ◽  
Bacillus Licheniformis ◽  
Basal Medium ◽  
Bacillus Species ◽  
Bacillus Sp ◽  
Feather Degradation ◽  
Feather Waste ◽  
Degrading Bacteria ◽  
Significant Difference

Aim: This study focuses on the screening and characterisation of keratin-degrading Bacillus species from feather waste. Methods: Nine bacteria were isolated from feather waste obtained from a poultry layout at Egbeda local government secretariat, Ibadan, Nigeria. These bacteria were grown in basal medium with feather as primary source of carbon, nitrogen, sulfur and energy. Feather degrading bacteria were screened for both proteolytic activity and keratin degradation on skimmed milk agar and keratin azure medium respectively. They were also screened for their ability to degrade other keratin substrates such as hair and nail. Results: Three of the isolates with higher feather degradation levels also showed high proteolytic activity and release of azure dye. They were selected and identified phenotypically and genotypically using 16S rRNA sequencing as Bacillus licheniformis-K51, Bacillus subtilis-K50 and Bacillus sp.-K53. The bacteria were capable of degrading other keratin-containing substrates such as nail and hair. Bacillus subtilis-K50 and Bacillus licheniformis-K51 showed significant difference (P) in degradation among the three different keratin sources used yielding higher degradation with feather as keratin source with respective optical densities of 0.07 and 0.11 followed by hair and least in nails with optical densities of 0.05 and 0.07 respectively. Highest degradation of all the three keratin substrates was observed in Bacillus licheniformis-K51. Conclusion: The three isolated bacteria possess the ability to degrade keratin and utilize feather as keratin substrate. As a result, these can be considered as potential candidates for degradation and utilization of feather keratin.

scholarly journals The Auxiliary Effect of Different Bacterial Groups on the Degradation of Organic Matter in Refinery Wastewater by Bacillus Licheniformis

CONVERTER ◽  
2021 ◽  
pp. 868-872
Author(s):  
Yong Chen, Yue Jiao, Xiao Li Wen
Keyword(s):  
Organic Matter ◽  
Bacillus Licheniformis ◽  
Biological Treatment ◽  
Ammonia Nitrogen ◽  
Degradation Efficiency ◽  
Refinery Wastewater ◽  
Petrochemical Wastewater ◽  
Dominant Bacteria ◽  
Bacterial Groups ◽  
Wastewater Biological Treatment

In this paper, the biodegradation of organic compounds in refinery wastewater was studied. Different microbial communities suitable for the treatment of refinery wastewater were selected to degrade the pretreated wastewater. The results showed that the degradation efficiency of COD and ammonia nitrogen was 59.4% and 56.7% respectively; the degradation efficiency of COD and ammonia nitrogen was 50.2% and 60.6% respectively. It can be used as the dominant bacteria for further domestication and cultivation of petrochemical wastewater biological treatment.

scholarly journals Bacillus stearothermophilus Neopullulanase Selective Hydrolysis of Amylose to Maltose in the Presence of Amylopectin

2002 ◽  
Vol 68 (4) ◽  
pp. 1658-1664 ◽  
Author(s):  
Hiroshi Kamasaka ◽  
Kazuhisa Sugimoto ◽  
Hiroki Takata ◽  
Takahisa Nishimura ◽  
Takashi Kuriki
Keyword(s):  
Substrate Specificity ◽  
Bacillus Licheniformis ◽  
Bacillus Megaterium ◽  
Bacillus Stearothermophilus ◽  
Main Product ◽  
Selective Hydrolysis ◽  
Amylolytic Enzymes ◽  
Order Of Magnitude ◽  
Maltogenic Amylase ◽  
Hydrolysis Of

ABSTRACT The specificity of Bacillus stearothermophilus TRS40 neopullulanase toward amylose and amylopectin was analyzed. Although this neopullulanase completely hydrolyzed amylose to produce maltose as the main product, it scarcely hydrolyzed amylopectin. The molecular mass of amylopectin was decreased by only one order of magnitude, from approximately 108 to 107 Da. Furthermore, this neopullulanase selectively hydrolyzed amylose when starch was used as a substrate. This phenomenon, efficient hydrolysis of amylose but not amylopectin, was also observed with cyclomaltodextrinase from alkaliphilic Bacillus sp. strain A2-5a and maltogenic amylase from Bacillus licheniformis ATCC 27811. These three enzymes hydrolyzed cyclomaltodextrins and amylose much faster than pullulan. Other amylolytic enzymes, such as bacterial saccharifying α-amylase, bacterial liquefying α-amylase, β-amylase, and neopullulanase from Bacillus megaterium, did not exhibit this distinct substrate specificity at all, i.e., the preference of amylose to amylopectin.

scholarly journals Biodegradation α-endosulfan and α-cypermethrin by Acinetobacter schindleri B7 isolated from the microflora of grasshopper (Poecilimon tauricola)

2021 ◽  
Author(s):  
Ozlem Gur Ozdal ◽  
Omer Faruk ALGUR
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Megaterium ◽  
Biological Treatment ◽  
Minimal Salt Medium ◽  
Salt Medium ◽  
Negative Bacterium ◽  
Pesticide Degradation ◽  
Gram Negative ◽  
Degrading Bacteria ◽  
Brevibacillus Parabrevis

Abstract Extensive applications of pesticides have led to the contamination of ecosystem. Therefore, the isolation of new pesticide degrading bacteria is important. For the biodegradation of α-endosulfan and α-cypermethrin, new bacteria isolates were isolated from grasshopper (Poecilimon tauricola). According to different tests, these isolated bacteria were identified as Pseudomonas aeruginosa B5, Acinetobacter johnsonii B6, Acinetobacter schindleri B7, Bacillus megaterium B9 and Brevibacillus parabrevis B12. The first two of these bacteria have been isolated as those that can use only α-endosulfan and the last two only use α-cypermethrin. Moreover, A. schindleri B7 was determined to be able to degrade both pesticides. When glucose was added to non-sulfur medium containing α-endosulfan (100 mg/L) and minimal salt medium containing α-cypermethrin (100 mg/L), both pesticide degradation and bacterial growth was increased. As a result, A. schindleri, a new gram negative bacterium, can inevitably be used in the biological treatment of environments exposed to pesticides.

scholarly journals Isolation of 2 simazine-degrading bacteria and development of a microbial agent for bioremediation of simazine pollution

2021 ◽  
Vol 93 (suppl 3) ◽  
Author(s):  
JIANGWEI ZHU ◽  
YAN ZHAO ◽  
XIAOLOU LI ◽  
LEI WU ◽  
LI FU ◽  
...  
Keyword(s):  
Degrading Bacteria ◽  
Microbial Agent

The use of microbiological fertilizers in planting early potatoes in the Belgorod region

2021 ◽  
pp. 30-33
Author(s):  
И.Н. Гаспарян ◽  
А.Г. Левшин ◽  
М.Е. Дыйканова ◽  
Н.Ф. Денискина ◽  
С.И. Смуров
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Bacillus Megaterium ◽  
Wide Spectrum ◽  
Azotobacter Chroococcum ◽  
Stress Factors ◽  
Early Potato ◽  
Negative Effects ◽  
Potato Varieties ◽  
Working Solution

Экологически безопасная продукция пользуется большим спросом. Для ее получения можно использовать микробиологические удобрения. Использование микробиологических удобрений способствует снижению негативных последствий изменения климата и повышает устойчивость растений картофеля к стрессовым факторам. Цель работы: изучить микробиологическое удобрение Славол, обладающее широким спектром действия, на ранних сортах картофеля и выявить его влияние на продуктивность этой культуры. Славол содержит в своем составе шесть компонентов микроорганизмов (Derxia spp., Azotobacter chroococcum, Azotobacter vinelandi, Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium). Работа была выполнена в 2020–2021 годах на участке научно-производственной лаборатории по изучению систем земледелия ФГБОУ ВО Белгородский ГАУ. Почва опытного участка – чернозем выщелоченный, среднемощный, среднесуглинистый на лессовидном суглинке. В опытах были использованы следующие сорта: Удача, Брянский деликатес, Ред Скарлетт, Жуковский ранний, Метеор, Ривьера. Варианты опыта: 1) контроль и 2) применение микробиологического удобрения Славол, которое включало обработку клубней перед посадкой (10 мл/л воды, замачивание на 3–4 часа) и в период ухода дважды в виде некорневой подкормки (10 мл/10 л воды, расход рабочего раствора – 1,5 л/10 м2), первая обработка – через 10–15 дней после всходов, вторая – в период бутонизации. Повторность опытов трехкратная. Варианты в опыте были размещены рендомизированным методом. Площадь одной опытной делянки – 25 м2. Схема посадки – 70×35 см. Установлено, что микробиологическое удобрение Славол обладает широким спектром действия и влияет на рост и развитие растений: повысилось количество стеблей (в среднем по сортам на 32,6%), увеличилась высота растений (на 10,8%) и общая площадь листового аппарата (на 32,9%). В посадках картофеля на 72,2% снизилось развитие альтернариоза. Комплексное влияние препарата сказа Environmentally friendly products are in great demand. Microbiological fertilizers can be used to obtain it. The use of microbiological fertilizers helps to reduce the negative effects of climate change and increases the resistance of potato plants to stress factors. The purpose of the work: to study the microbiological fertilizer Slavol, which has a wide spectrum of action, on early potato varieties, and to identify its effect on the productivity of this crop. Slavol contains six components of microorganisms (Derxia spp., Azotobacter chroococcum, Azotobacter vinelandi, Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium). The work was carried out in 2020–2021 at the site of the scientific and production laboratory for the study of agricultural systems of the Belgorod State Agrarian University. The soil of the experimental site is leached chernozem, medium-thick, medium-loamy on loess-like loam. The following varieties were used in the experiments: Udacha, Bryansk delikates, Red Scarlett, Zhukovsky ranniy, Meteor, Riviera. Experience options: 1) control and 2) the use of microbiological fertilizer Slavol, which included the treatment of tubers before planting (10 ml/l of water, soaking for 3–4 hours) and during the care period twice in the form of foliar top dressing (10 ml/10 l of water, the consumption of the working solution of 1.5 l/10 m2) the first treatment 10–15 days after germination, the second – during budding. The repetition of experiments is threefold. The variants in the experiment were placed by a randomized method. The area of one experimental plot is 25 m2. The planting scheme is 70×35 cm. It is established that the microbiological fertilizer Slavol has a wide spectrum of action and affects the growth and development of plants: the number of stems has increased (on average for varieties by 32.6%), the height of plants has increased (by 10.8%) and the total area of the leaf apparatus (by 32.9%). In potato plantings, the development of alternariosis decreased by 72.2%. The complex effect of the drug affected the yield of early potato varieties, which increased by an average of 12.3% in the Belgorod region.

Isolation and Characterization of Hydrocarbon-degrading Bacteria in Soils of Mechanical Workshops in Maiduguri, Borno State

2021 ◽  
Vol 4 (2) ◽  
pp. 35-38
Author(s):  
Hussaini Shettima ◽  
Ibrahim Alkali Allamin ◽  
Nasir Halima ◽  
Haruna Yahya Ismail ◽  
Yusuf Musa
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Heterotrophic Bacteria ◽  
Bacterial Species ◽  
Bacillus Coagulans ◽  
Engine Oil ◽  
Klebsiella Pneumonia ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Hydrocarbon Utilizing Bacteria

The study was conducted to determine the distribution of hydrocarbon utilizing bacteria in spent engine oil (SEO) contaminated soil. Five mechanical workshops within Maiduguri Metropolis. Five bulk soil sample comprising of one each of the five sites; the sites are Leventis Area on Kashim Ibrahim Way, Damboa Raod, Boiler (commonly called Bola) near Maiduguri Monday Market, Ngomari, on Kano Road, and 1000 Housing Estate, also on Kano Road. Nutrient agar was used, or isolation and enumeration total heterotrophic bacteria and Mineral salt agar was used for the isolation and enumeration of hydrocarbon utilizing bacteria. The result shows THB (92.0x104) at Damboa road (DR) to as higher and (78.8x104) at Bola area (BL) while HUB (2.0x104) at Damboa road (DR) and as high as (9.3x104) at Bola area (BL). The bacterial species isolated were species of Bacillus licheniformis, Bacillus subtilis, Bacillus coagulans, Bacillus alvei, Bacillus cereus, Bacillus lentus other are Pseudomonas aeruginosa, Klebsiella pneumonia. Bacillus licheniformis, and Bacillus subtilis are the most occurring bacterial isolates identified. The ability of those bacterial isolate to degrade hydrocarbon buoyantly will help in remediation of oil polluted environments.

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