scholarly journals Microbiome of the Queensland Fruit Fly through Metamorphosis

2020 ◽  
Vol 8 (6) ◽  
pp. 795 ◽  
Author(s):  
Rajib Majumder ◽  
Brodie Sutcliffe ◽  
Phillip W. Taylor ◽  
Toni A. Chapman
Keyword(s):  
Microbial Communities ◽  
Developmental Stages ◽  
Pupal Stage ◽  
Fruit Fly ◽  
Adult Males ◽  
The Family ◽  
Nutritional Biology ◽  
Laboratory Colonies ◽  
Bacteria And Fungi ◽  
Next Generation Sequencing Ngs

Bactrocera tryoni (Froggatt) (Queensland fruit fly, or “Qfly”) is a highly polyphagous tephritid fruit fly and a serious economic pest in Australia. Qfly biology is intimately linked to the bacteria and fungi of its microbiome. While there are numerous studies of the microbiome in larvae and adults, the transition of the microbiome through the pupal stage remains unknown. To address this knowledge gap, we used high-throughput Next-Generation Sequencing (NGS) to examine microbial communities at each developmental stage in the Qfly life cycle, targeting the bacterial 16S rRNA and fungal ITS regions. We found that microbial communities were similar at the larval and pupal stage and were also similar between adult males and females, yet there were marked differences between the larval and adult stages. Specific bacterial and fungal taxa are present in the larvae and adults (fed hydrolyzed yeast with sugar) which is likely related to differences in nutritional biology of these life stages. We observed a significant abundance of the Acetobacteraceae at the family level, both in the larval and pupal stages. Conversely, Enterobacteriaceae was highly abundant (>80%) only in the adults. The majority of fungal taxa present in Qfly were yeasts or yeast-like fungi. In addition to elucidating changes in the microbiome through developmental stages, this study characterizes the Qfly microbiome present at the establishment of laboratory colonies as they enter the domestication process.

Life stages and phylogenetic position of the wool carder bee mite Sennertionyx manicati (Acari: Acaridae)

Zootaxa ◽  
2020 ◽  
Vol 4857 (1) ◽  
pp. 160-195
Author(s):  
QING-HAI FAN ◽  
DONGMEI LI ◽  
SHERLY GEORGE
Keyword(s):  
Phylogenetic Analysis ◽  
New Zealand ◽  
Molecular Analysis ◽  
Phylogenetic Trees ◽  
Developmental Stages ◽  
Phylogenetic Position ◽  
Life Stages ◽  
Adult Males ◽  
The Family ◽  
Anthidium Manicatum

In mites of the family Acaridae, usually two developmental stages, adult (males, females) and deutonymphal stage (=hypopus) are used for diagnostic descriptions. Because these taxonomically important stages live in different habitats (i.e. deutonymphs are phoretic on a hymenopteran host, while adults live inside the nest of the host), one of these can be missing or they cannot be confidently corelated with each other. Sennertionyx manicati (Acari: Acaridae) was only known from hypopus. Herein, we describe all post-embryonic stages of S. manicati based on material obtained from a nest of Anthidium manicatum in Auckland, New Zealand, and provide a key to all life stages of this species. We also conducted a phylogenetic analysis of the 18S gene and the D2/D3 region of 28S genes from field-collected specimens and GenBank data. The molecular analysis indicated the New Zealand specimen shared the identical 18S and 99.5% identity of the D2D3 region of 28S sequences with S. manicati from Italy, and clustered together in the phylogenetic trees. 

scholarly journals Microbial Communities in Different Developmental Stages of the Oriental Fruit Fly, Bactrocera dorsalis, Are Associated with Differentially Expressed Peptidoglycan Recognition Protein-Encoding Genes

2019 ◽  
Vol 85 (13) ◽  
Author(s):  
Hongmei Huang ◽  
Huijing Li ◽  
Lu Ren ◽  
Daifeng Cheng
Keyword(s):  
Microbial Communities ◽  
Developmental Stages ◽  
Fruit Fly ◽  
Subtractive Hybridization ◽  
Bactrocera Dorsalis ◽  
Gram Negative Bacteria ◽  
Oriental Fruit Fly ◽  
Content Type ◽  
Development Stages ◽  
Gram Positive Bacteria

ABSTRACT The insect microbiota can change dramatically to enable adaptation of the host in different developmental stages and environments; however, little is known about how the host maintains its microbiota to achieve such adaptations. In this study, 16S rRNA sequencing revealed that the microorganisms in larvae and adults of the Oriental fruit fly, Bactrocera dorsalis, are primarily Gram-negative bacteria but that the major components in pupae are Gram-positive bacteria. Using suppression subtractive hybridization (SSH) and transcriptome analysis, we screened two specifically expressed genes encoding peptidoglycan recognition proteins (PGRP-LB and PGRP-SB1) and analyzed their relationship to B. dorsalis microbial communities. Knockdown of the PGRP-LB gene in larvae and adults led to increased ratios of Gram-positive bacteria; knockdown of the PGRP-SB1 gene in pupae led to increased ratios of Gram-negative bacteria. Our results suggest that maintenance of the microbiota in different developmental stages of B. dorsalis may be associated with the PGRP-LB and PGRP-SB1 genes. IMPORTANCE Microorganisms are ubiquitous in insects and have widespread impacts on multiple aspects of insect biology. However, the microorganisms present in insects can change dramatically in different developmental stages, and it is critical to maintain the appropriate microorganisms in specific host developmental stages. Therefore, analysis of the factors associated with the microbiota in specific development stages of the host is needed. In this study, we applied suppression subtractive hybridization (SSH) combined with transcriptome analysis to investigate whether the microbiota in development stages of the Oriental fruit fly, Bactrocera dorsalis, is associated with expression of PGRP genes. We found that two different PGRP genes were specifically expressed during development and that these genes may be associated with changes in microbial communities in different developmental stages of B. dorsalis.

Effect of gamma-radiation on the tsetse fly Glossina Morsitans Westw

1969 ◽  
Vol 58 (3) ◽  
pp. 505-519 ◽  
Author(s):  
G. J. Dean ◽  
S. M. Wortham
Keyword(s):  
Gamma Radiation ◽  
Emerging Adults ◽  
Developmental Stages ◽  
Pupal Stage ◽  
Tsetse Fly ◽  
Adult Males ◽  
Glossina Morsitans ◽  
Mating Period ◽  
Continuous Irradiation ◽  
Complete Failure

Puparia of Glossina morsitans Westw. were collected in the Zambesi valley and contained developmental stages from final phases of third-instar larvae to pupae near to eclosion. These puparia or the emerging adults were exposed to gamma-radiation from a Co60 source at rates between 54 and 122 rad/min. Effective mortality in the pupal stage increased from 23 per cent, at 2,000 rads to 64 per cent, at 15,000 rads, while those deposited in spring (August-October) were more sensitive to gamma-rays than those collected in winter (May-July).Under described test conditions, 25 pairs of untreated flies produced a mean of 21 pupae in a 28-day test period, insemination averaged 95 per cent., and 56 and 62 per cent, of the males and females survived, respectively. Continuous irradiation reduced reproduction by over 95 per cent, between 8,000 and 15,000 rads wiih males emerging within the first two weeks after treatment; while males emerging during the third post-treatment week were completely sterilised with dosages above 4,000 rads. Treatments given as fractions one and four days apart gave a slightly higher level of residual fertility than that obtained with similar but continuous total dosages. Irradiation of adult males reduced reproduction to an extent comparable with that observed in males emerging from pupae within two weeks of treatment (> 90 per cent, from 8,000 rads). Females treated either as adults or as pupae were completely sterilised with doses of 1,000 to 8,000 rads. Males exposed to 8,000 and 12,000 rads retained their sterility throughout 39- and 45-day test periods, and were fully competitive with untreated males.Survival of virgin flies emerging from treated pupae was reduced both by increasing dosage from 4,000 to 15,000 rads, and decreasing pupal age at the time of treatment. Mortality during the 28-day mating period decreased with 1,000 and 2,000 rads, increased up to 8,000 rads, and apparently decreased with 12,000 and 15,000 rads. When the doses were fractionated, survival was longer than when treatment was continuous, and mortality was reduced to the level expected from the size of the first fraction. Virgin adult males given doses between 4,000 and 18,000 rads, and females given 2,000 and 4,000 rads, lived longer than the corresponding controls.The ability of the males to inseminate females was not affected by any of the treatments, and sperm from irradiated males were mobile and apparently behaved normally. Dominant lethality in the males was expressed by the failure to produce pupae in matings with untreated females, and in the treated female fly by the complete failure of oogenesis.

scholarly journals Changes in rhizosphere soil microbial communities across plant developmental stages of high and low methane emitting rice genotypes

2021 ◽  
pp. 108233
Author(s):  
Cristina P. Fernández-Baca ◽  
Adam R. Rivers ◽  
Woojae Kim ◽  
Ryo Iwata ◽  
Anna M. McClung ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Developmental Stages ◽  
Rhizosphere Soil ◽  
Soil Microbial Communities ◽  
Soil Microbial ◽  
Rice Genotypes

scholarly journals Structure of Microbial Communities When Complementary Effluents Are Anaerobically Digested

2021 ◽  
Vol 11 (3) ◽  
pp. 1293
Author(s):  
Ana Eusébio ◽  
André Neves ◽  
Isabel Paula Marques
Keyword(s):  
Anaerobic Digestion ◽  
Microbial Communities ◽  
Volatile Fatty Acids ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
Rrna Gene ◽  
Bacterial Populations ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Olive Mill

Olive oil and pig productions are important industries in Portugal that generate large volumes of wastewater with high organic load and toxicity, raising environmental concerns. The principal objective of this study is to energetically valorize these organic effluents—piggery effluent and olive mill wastewater—through the anaerobic digestion to the biogas/methane production, by means of the effluent complementarity concept. Several mixtures of piggery effluent were tested, with an increasing percentage of olive mill wastewater. The best performance was obtained for samples of piggery effluent alone and in admixture with 30% of OMW, which provided the same volume of biogas (0.8 L, 70% CH4), 63/75% COD removal, and 434/489 L CH4/kg SVin, respectively. The validation of the process was assessed by molecular evaluation through Next Generation Sequencing (NGS) of the 16S rRNA gene. The structure of the microbial communities for both samples, throughout the anaerobic process, was characterized by the predominance of bacterial populations belonging to the phylum Firmicutes, mainly Clostridiales, with Bacteroidetes being the subdominant populations. Archaea populations belonging to the genus Methanosarcina became predominant throughout anaerobic digestion, confirming the formation of methane mainly from acetate, in line with the greatest removal of volatile fatty acids (VFAs) in these samples.

scholarly journals Pathogenicity and Side Effect of Indigenous Beauveria bassiana on Coccinella undecimpunctata and Hippodamia variegata (Coleoptera: Coccinellidae)

Insects ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 42
Author(s):  
Samy Sayed ◽  
Sayed-Ashraf Elarrnaouty ◽  
Saad AlOtaibi ◽  
Mohamed Salah
Keyword(s):  
Beauveria Bassiana ◽  
Indirect Effect ◽  
Developmental Stages ◽  
Larval Instar ◽  
Pupal Stage ◽  
Tween 80 ◽  
Contact Method ◽  
Coccinella Undecimpunctata ◽  
Hippodamia Variegata ◽  
Application Methods

This study aimed to estimate the virulence of an indigenous Beauveria bassiana on all developmental stages of two indigenous coccinellids; Coccinella undecimpunctata and Hippodamia variegata through three application methods; direct spray, contact method, and feeding on aphids treated with the fungus (ingestion). Also, indirect effect on all developmental stages resulted from 1st larval instar treated with these application methods. All treatments were done with a concentration of 1 × 105 which was recommended in previous studies for different aphid species with a control of 0.02% Tween 80 (v/v). The mortality of 1st larval instar of both H. variegata and C. undecimpunctata and pupal stage of C. undecimpunctata were significantly increased with spray method only. Also, contact method achieved significantly higher mortality on 1st larval instar of C. undecimpunctata only. Regard to indirect effect, except of mortality of 1st larval instar of both predators and 2nd larval instar of H. variegata, other developmental instars/stages of both predators were not affected by B. bassiana through the three tested application methods in the mortality, duration, survival, cumulative survival male and female longevity, and fecundity. Therefore, both tested predatory coccinellids could be compatible with this indigenous isolate of B. bassiana where, in general, there are no negative effects of the fungus on both predators.

scholarly journals Transcriptional Profiles of Secondary Metabolite Biosynthesis Genes and Cytochromes in the Leaves of Four Papaver Species

Data ◽  
2018 ◽  
Vol 3 (4) ◽  
pp. 55 ◽  
Author(s):  
Dowan Kim ◽  
Myunghee Jung ◽  
In Ha ◽  
Min Lee ◽  
Seok-Geun Lee ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Bioinformatics Analyses ◽  
Kegg Pathways ◽  
Benzylisoquinoline Alkaloid ◽  
Development Life Cycle ◽  
The Family ◽  
Leaf Transcriptome ◽  
Secondary Metabolite Biosynthesis

Poppies are well-known plants in the family Papaveraceae that are rich in alkaloids. This family contains 61 species, and in this study we sequenced the transcriptomes of four species’ (Papaver rhoeas, Papaver nudicaule, Papaver fauriei, and Papaver somniferum) leaves. These transcripts were systematically assessed for the expression of secondary metabolite biosynthesis (SMB) genes and cytochromes, and their expression profiles were assessed for use in bioinformatics analyses. This study contributed 265 Gb (13 libraries with three biological replicates) of leaf transcriptome data from three Papaver plant developmental stages. Sequenced transcripts were assembled into 815 Mb of contigs, including 226 Mb of full-length transcripts. The transcripts for 53 KEGG pathways, 55 cytochrome superfamilies, and benzylisoquinoline alkaloid biosynthesis (BIA) were identified and compared to four other alkaloid-rich genomes. Additionally, 22 different alkaloids and their relative expression profiles in three developmental stages of Papaver species were assessed by targeted metabolomics using LC-QTOF-MS/MS. Collectively, the results are given in co-occurrence heat-maps to help researchers obtain an overview of the transcripts and their differential expression in the Papaver development life cycle, particularly in leaves. Moreover, this dataset will be a valuable resource to derive hypotheses to mitigate an array of Papaver developmental and secondary metabolite biosynthesis issues in the future.

scholarly journals The Scent of Ant Brood: Caste Differences in Surface Hydrocarbons of Formica exsecta Pupae

2021 ◽  
Author(s):  
Unni Pulliainen ◽  
Nick Bos ◽  
Patrizia d’Ettorre ◽  
Liselotte Sundström
Keyword(s):  
Surface Chemistry ◽  
Chemical Communication ◽  
Cuticular Hydrocarbons ◽  
Social Insects ◽  
Developmental Stages ◽  
Pupal Stage ◽  
Male And Female ◽  
Chemical Stimuli ◽  
Caste Differences ◽  
Long Chain

AbstractChemical communication is common across all organisms. Insects in particular use predominantly chemical stimuli in assessing their environment and recognizing their social counterparts. One of the chemical stimuli used for recognition in social insects, such as ants, is the suite of long-chain, cuticular hydrocarbons. In addition to providing waterproofing, these surface hydrocarbons serve as a signature mixture, which ants can perceive, and use to distinguish between strangers and colony mates, and to determine caste, sex, and reproductive status of another individual. They can be both environmentally and endogenously acquired. The surface chemistry of adult workers has been studied extensively in ants, yet the pupal stage has rarely been considered. Here we characterized the surface chemistry of pupae of Formica exsecta, and examine differences among sexes, castes (reproductive vs. worker), and types of sample (developing individual vs. cocoon envelope). We found quantitative and qualitative differences among both castes and types of sample, but male and female reproductives did not differ in their surface chemistry. We also found that the pupal surface chemistry was more complex than that of adult workers in this species. These results improve our understanding of the information on which ants base recognition, and highlights the diversity of surface chemistry in social insects across developmental stages.

Extrinsic Inter- and Intra-specific Competition in Parasitoid Wasps

2021 ◽  
Vol 67 (1) ◽  
Author(s):  
Paul J. Ode ◽  
Dhaval K. Vyas ◽  
Jeffrey A. Harvey
Keyword(s):  
Developmental Stages ◽  
Interference Competition ◽  
Apparent Competition ◽  
Annual Review ◽  
Publication Date ◽  
Natural Ecosystem ◽  
Adult Males ◽  
Alternate Host ◽  
Adult Females ◽  
Behavioral Mechanisms

The diverse ecology of parasitoids is shaped by extrinsic competition, i.e., exploitative or interference competition among adult females and males for hosts and mates. Adult females use an array of morphological, chemical, and behavioral mechanisms to engage in competition that may be either intra- or interspecific. Weaker competitors are often excluded or, if they persist, use alternate host habitats, host developmental stages, or host species. Competition among adult males for mates is almost exclusively intraspecific and involves visual displays, chemical signals, and even physical combat. Extrinsic competition influences community structure through its role in competitive displacement and apparent competition. Finally, anthropogenic changes such as habitat loss and fragmentation, invasive species, pollutants, and climate change result in phenological mismatches and range expansions within host–parasitoid communities with consequent changes to the strength of competitive interactions. Such changes have important ramifications not only for the success of managed agroecosystems, but also for natural ecosystem functioning. Expected final online publication date for the Annual Review of Entomology, Volume 67 is January 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Biological control methods in Piper nigrum Linn. leaves infected with galls caused by Liothrips karneyi

2021 ◽  
Vol 12 (1) ◽  
pp. 696-698
Author(s):  
Asha Renjith ◽  
Payal Lodha
Keyword(s):  
Biological Control ◽  
Disease Management ◽  
Crop Production ◽  
Developmental Stages ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Pharmacological Activities ◽  
The Family ◽  
Growth Reaction ◽  
Complex Phenomena

Piper nigrum Linn. (Black pepper)  belongs to the family Piperaceae and an economically and medicinally important spice and is a native of Southern India. Leaf marginal gall is transmitted by Liothrips karnyi belonging to family Phlaeothripidae of order Thysanoptera (Thrips). Insect induced galls arise due to growth reaction of plants due to the attack of cecidozoa, also known as zoocecidia. The growth and development of the host plant alter due to the infestation of the insect, which involves certain complex phenomena. The different groups of cecidozoa influence the plants in such a way as to produce galls at different stages in their life history. Most of the cecidozoa cause galls only in their developmental stages, but some of them are capable of inducing galls both in larval as well as adult stages. The crop production in India has been adversely affected due to the infestation of pests. And it is necessary to use different approaches of disease management to curb the infestation, and significant researchers have shown progress to protect the crops in an eco-friendly manner. Piperine exhibits diverse pharmacological activities like antihypertensive, antiplatelets, antioxidant, antitumor, antipyretic, analgesic, anti-inflammatory, anti-diarrheal, antibacterial, antifungal, anti-reproductive, insecticidal activities. In the present investigation, the biological method of disease management of marginal leaf gall transmitted by Liothrips karnyi has been undertaken. The biological control of leaf diseases has been significant on the increased reflection of environmental problems over pesticide uses.

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