scholarly journals Effect of Weakly Basic Conditions on the Separation and Purification of Flavonoids and Glycosides from Chrysanthemum morifolium Tea

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 297 ◽  
Author(s):  
Yuxiao Wang ◽  
Zhenzhen Xu ◽  
Yue Wu ◽  
Mo Li ◽  
Sicheng Pang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Quinic Acid ◽  
Ultra Performance Liquid Chromatography ◽  
Chrysanthemum Morifolium ◽  
Separation And Purification ◽  
Caffeoylquinic Acids ◽  
Flight Mass Spectrometry ◽  
Functional Aspects ◽  
Ammonium Hydrogen

Tea brewed from chrysanthemum flowers has been widely used in Chinese medicine. The possibly medicinal compounds in Chrysanthemum morifolium tea can be purified by preparative high performance liquid chromatography (HPLC), but this is usually done with acidic conditions, which leads to the hydrolysis of glycosides. In hopes of avoiding this hydrolysis, we explored the effect of weakly basic conditions on the separation and purification of flavonoids and glycosides from Chrysanthemum morifolium. We also explored the effects of weakly basic conditions on chlorogenic acid (3-CQA) and apigenin-7-O-glucoside (A7G). Our results show that the concentration of the weakly basic ammonium hydrogen carbonate and time had no significant effect on A7G, p < 0.01, but it had a significant effect on 3-CQA, p < 0.01. HPLC and ultraviolet (UV) analysis showed that the structure of 3-CQA is destroyed in weakly basic conditions. Caffeic acid, quinic acid, and 3,4-dihydroxymandelic acid, which is a hydrolysate of 3-CQA, were identified by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The results showed that weakly basic conditions could be used for the purification of flavonoids and glycosides but not for caffeoylquinic acids. Moreover, our work clarified the hydrolysis behaviour of caffeoylquinic acids, which can be helpful for research into their functional aspects.

scholarly journals Acylated Flavonoid Glycosides are the Main Pigments that Determine the Flower Colour of the Brazilian Native Tree Tibouchina pulchra (Cham.) Cogn.

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 718 ◽  
Author(s):  
Fernanda Rezende ◽  
Marcelo Ferreira ◽  
Mads Clausen ◽  
Magdalena Rossi ◽  
Claudia Furlan
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Flower Colour ◽  
Metabolite Profile ◽  
Ultra Performance Liquid Chromatography ◽  
Flavonoid Glycosides ◽  
Array Detector ◽  
Flight Mass Spectrometry ◽  
Photodiode Array Detector ◽  
Photodiode Array

Tibouchina pulchra (Cham.) Cogn. is a plant native to Brazil whose genus and family (Melastomataceae) are poorly studied with regards to its metabolite profile. Phenolic pigments of pink flowers were studied by ultra-performance liquid chromatography with a photodiode array detector and electrospray ionization quadrupole time-of-flight mass spectrometry. Therein, twenty-three flavonoids were identified with eight flavonols isolated by preparative high-performance liquid chromatography and analysed by one- and two-dimensional nuclear magnetic resonance. Kaempferol derivatives were the main flavonols, encompassing almost half of the detected compounds with different substitution patterns, such as glucoside, pentosides, galloyl-glucoside, p-coumaroyl-glucoside, and glucuronide. Concerning the anthocyanins, petunidin p-coumaroyl-hexoside acetylpentoside and malvidin p-coumaroyl-hexoside acetylpentoside were identified and agreed with previous reports on acylated anthocyanins from Melastomataceae. A new kaempferol glucoside was identified as kaempferol-(2′′-O-methyl)-4′-O-α-d-glucopyranoside. Moreover, twelve compounds were described for the first time in the genus with five being new to the family, contributing to the chemical characterisation of these taxa.

scholarly journals Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of Zanthoxylum nitidum Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 585 ◽  
Author(s):  
Aihua Huang ◽  
Yuguang Chi ◽  
Jiawei Liu ◽  
Mincun Wang ◽  
Jialiang Qin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Oral Administration ◽  
High Performance ◽  
Ultra Performance Liquid Chromatography ◽  
Rat Plasma ◽  
Pharmacokinetic Studies ◽  
Flight Mass Spectrometry ◽  
Zanthoxylum Nitidum

Zanthoxylum nitidum (Roxb.) DC (Rutaceae), called as “liangmianzhen” in China, is well known for its anti-inflammation and analgesic effect. Alkaloids are its main active constituents. However, little has been known about the absorption of main alkaloids in vivo. In this study, an ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry was employed for identification of absorbed alkaloids in rats after oral administration of Z. nitidum decoction. By analyzing the fragmentation patterns, a total of nineteen alkaloids were exactly or tentatively identified in rat plasma after treatment, of which magnoflorine, α-allocryptopine, and skimmianine are dominant. Moreover, a high performance liquid chromatography coupled mass spectrometry method was developed for simultaneous quantification of magnoflorine, α-allocryptopine, and skimmianine, and successfully applied to pharmacokinetic study in rats after oral administration of Z. nitidum decoction. The research would contribute to comprehensive understanding of the material basis and function mechanism of Z. nitidum decoction.

scholarly journals Purification, Identification, and Sensory Evaluation of Kokumi Peptides from Agaricus bisporus Mushroom

Foods ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Tao Feng ◽  
Yang Wu ◽  
Zhiwen Zhang ◽  
Shiqing Song ◽  
Haining Zhuang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Sensory Evaluation ◽  
Agaricus Bisporus ◽  
High Performance ◽  
Solid Phase ◽  
Gel Permeation Chromatography ◽  
Ultra Performance Liquid Chromatography ◽  
Taste Intensity ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Agaricus bisporus can enhance the umami and salty taste in chicken soup, and also has a high protein content, which indicates that there might be some kokumi taste compounds in this mushroom. Therefore, through ultrafiltration, gel permeation chromatography (GPC), and reverse phase-high performance liquid chromatography (RP-HPLC), some peptides in fresh Agaricus bisporus mushroom were isolated. Then, these peptides were identified by sensory evaluation and ultra performance liquid chromatography (UPLC) coupled quadruple time of flight mass spectrometry (Q-TOF-MS). The sensory evaluation results showed that the addition of aqueous extract isolated from Agaricus bisporus to model chicken broth did enhance chicken broth’s complexity, mouthfulness, and palatability. UPLC-Q-TOF-MS analysis found that Gly-Leu-Pro-Asp (Mw = 399.99) and Gly-His-Gly-Asp (Mw = 383.99) might act as key molecules to cause kokumi taste. In order to verify the kokumi taste of the above two peptides, they were synthesized by solid-phase synthesis and the taste properties of these two peptides were further characterized by descriptive sensory evaluation and taste intensity tests. This work indicated that it was feasible to produce kokumi peptides from Agaricus bisporus.

scholarly journals Phytochemical Profile and Antidepressant Effect of Ormosia henryi Prain Leaf Ethanol Extract

2019 ◽  
Vol 20 (14) ◽  
pp. 3396
Author(s):  
Ying Lu ◽  
Shihao Zhu ◽  
Yingjie He ◽  
Changfu Peng ◽  
Zhi Wang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Speed ◽  
High Performance ◽  
Ethanol Extract ◽  
Antidepressant Activity ◽  
Ultra Performance Liquid Chromatography ◽  
Antidepressant Effect ◽  
Mild Stress ◽  
Flight Mass Spectrometry ◽  
Phytochemical Profile

The Ormosia henryi Prain leaf (OHPL) is a new bioactive resource with potential antidepressant activity, but few reports have confirmed its chemical composition or antidepressant effect. To investigate the phytochemical profile of OHPL ethanol extract (OHPLE), six flavone C-glycosides and two flavone O-glycosides were purified by high-speed counter-current chromatography combined with preparative high-performance liquid chromatography (HSCCC-prep-HPLC). The eight isolated compounds were identified by NMR and MS. Forty-six flavonoids, including flavones, flavone C-glycosides, flavone O-glycosides, isoflavones, isoflavone O-glycosides, prenylflavones and polymethoxyflavones were definitively or tentatively identified from OHPLE using ultra-performance liquid chromatography/ electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS/MS) on the basis of fragment ions that are characteristic of these isolated compounds. The results of the antidepressant assay suggest that OHPLE significantly improved depression-related behaviors of chronic unpredictable mild stress (CUMS) mice. The observed changes in these mice after OHPLE treatment were an increased sucrose preference index, reduced feeding latency, prolonged tail suspension time, and upregulated expression of brain-derived neurotrophic factor (BDNF). The details of the phytochemicals and the antidepressant effect of OHPLE are reported here for the first time. This study indicates that the OHPL, enriched in flavone C-glycosides, is a new resource that might be potentially applied in the field of nutraceuticals (or functional additives) with depression-regulating functions.

Metabolic profile of dendrobine in rats determined by ultra-high performance liquid chromatography/Quadrupole time-of-flight mass spectrometry

2020 ◽  
Vol 23 ◽  
Author(s):  
Jun-qi Bai ◽  
Qian-xiang Guo ◽  
Jing Zhang ◽  
Juan Huang ◽  
Wen Xu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Metabolite Profiling ◽  
High Performance ◽  
Time Of Flight ◽  
Fragmentation Pathway ◽  
Ultra Performance Liquid Chromatography ◽  
Rat Plasma ◽  
Flight Mass Spectrometry

Aims & Objective: Dendrobine is a major alkaloid present mainly in dendrobium nobile Lindl. It has been reported to have analgesic, antipyretic, lower heart rate and blood pressure and other pharmacologic activities. Despite its critical pharmacological function, its metabolite profiling is still unclear. Methods: In this study, the in vivo metabolite profiling of dendrobine in rats was investigated using ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS). The metabolites were predicted using MetabolitePilotTM software with mass defect filter (MDF) technique. These predicted metabolites were further analyzed by MS2 spectra, and compared with the detailed fragmentation pathway of the dendrobine standard and literature data. Results: total of 59 metabolites were identified for the first time in rat plasma and urine after oral administration of dendrobine. Demethylated, dehydrogenated, hydroxylated, ketonizated and glucuronide were the major metabolic pathways. Conclusions: This research provides scientific and reliable support for full understanding of the metabolic fate of dendrobine in vivo.

scholarly journals Quantification of Lansoprazole in Oral Suspension by Ultra-High-Performance Liquid Chromatography Hybrid Ion-Trap Time-of-Flight Mass Spectrometry

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Stacy D. Brown ◽  
Justin D. Connor ◽  
Nicholas C. Smallwood ◽  
Ralph A. Lugo
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Ion Trap ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometric ◽  
Oral Suspension ◽  
Flight Mass Spectrometry ◽  
The Stability ◽  
Unique Mass

An LC-MS/MS method was developed and validated to be used as a stability indicating assay for the study of a 3 mg/mL lansoprazole oral suspension. The method utilizes a UPLC (ultra-performance liquid chromatography) column and unique mass spectrometric detection (ion-trap time-of-flight (IT-TOF)) to achieve a sensitive (LOD 2 ng/mL), accurate, and reproducible quantification of lansoprazole. This method reports an intraday and interday coefficient of variation of 2.98 ± 2.17% (n=5for each concentration for each day) and 3.07 ± 0.89% (n=20for each concentration), respectively. Calibration curves (5–25 μg/mL) were found to be linear with anR2value ranging from 0.9972 to 0.9991 on 4 different days. Accuracy of the assay, expressed as % error, ranged from 0.30 to 5.22%. This method is useful for monitoring the stability of lansoprazole in oral suspension.

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