scholarly journals Diversity in Accessions of Panicum miliaceum L. Based on Agro-Morphological, Antioxidative, and Genetic Traits

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1012 ◽  
Author(s):  
Bimal Ghimire ◽  
Chang Yu ◽  
Seung Kim ◽  
Ill-Min Chung
Keyword(s):  
Genetic Diversity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Panicum Miliaceum ◽  
Expected Heterozygosity ◽  
Radical Scavenging Capacity ◽  
Bioactive Properties ◽  
Scavenging Capacity

The genetic diversity and antioxidant potential of Panicum miliaceum L. accessions collected from different geo-ecological regions of South Korea were evaluated and compared. Antioxidant potential of seeds was estimated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging assays and total phenolic content was determined by the Folin–Ciocalteu method. Total phenolic content (TPC) in 80% methanolic extracts ranged from 16.24 ± 0.86 to 58.04 ± 1.00 mg gallic acid equivalent (GAE)/g of the sample extracts and total flavonoid content (TFC) varied from 7.19 ± 1.05 to 52.56 ± 1.50 mg quercetin equivalents (QE) mg/g of the sample extracts. DPPH radical scavenging capacity of the extracts from the 15 accessions of P. miliaceum varied from 206.44 ± 7.72 to 2490.24 ± 4.641 mg GAE/g of the sample extracts and ABTS radical scavenging capacity ranged from 624.85 ± 13.1 to 1087. 77 ± 9.58 mg GAE/g of the sample extracts. A wide range of genetic variation was observed as measured by Shannon’s information index (I), number of effective alleles (Ne), number of observed alleles (Na), expected heterozygosity (He), unbiased expected heterozygosity (uHe). The observed variation in the bioactive properties, morphological traits, and genetic diversity among the accessions may provide useful information for breeding programs seeking to improve bioactive properties of P. miliaceum.

Effect of 1-MCP on Bioavailability of Lingwu Long Jujube In Vitro Digestion

2012 ◽  
Vol 554-556 ◽  
pp. 1357-1360
Author(s):  
Zhong Li Jiang ◽  
Ai Li Wang ◽  
Xi Hong Li ◽  
Min Peng Zhu ◽  
Jun Wei Wang
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Control Samples

The present study investigated the effect of 1-MCP on bioavailability of Lingwu long jujube stored at 0 °C for 60 days. At the end of the storage, compared with the control samples, Lingwu long jujube treated with 1-MCP exhibited higher Vc bioaccessibility, total phenolic content and free radical scavenging capacity, which demonstrated that 1-MCP treatment could provide a better effect on maintaining the bioavailability of Lingwu long jujube.

scholarly journals Total Phenolic Content and Antioxidant Activity of Salvia spp. Exudates

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Laura Giamperi ◽  
Anahi Bucchini ◽  
Angela Bisio ◽  
Emanuela Giacomelli ◽  
Giovanni Romussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Dpph Radical Scavenging

The total phenolic content and antioxidant activity of 6 Salvia spp. exudates were measured to find new potential sources of natural antioxidants. Total phenolic content was assessed by a modified Prussian blue method, and the antioxidant activity by two methods: 1,1- diphenyl-2-picryl- hydrazyl (DPPH) radical scavenging capacity assay and lipoxygenase inhibitory assay. The total phenolic content ranged between 1.3 μg/mg DW ( S. fallax) and 74.0 μg/mg DW ( S. cacaliaefolia). In the DPPH test, S. cacaliaefolia was more effective than BHT, while in the inhibition of lipid peroxidation all the extracts presented good antioxidant capacity.

scholarly journals Influence of Sodium and Maturity Stage on the Antioxidant Properties of Cauliflower and Broccoli Sprouts

2017 ◽  
Vol 45 (2) ◽  
pp. 458-465 ◽  
Author(s):  
Antoanela PATRAS ◽  
Vasile STOLERU ◽  
Razvan Vasile FILIMON ◽  
Silvica PADUREANU ◽  
Elena Liliana CHELARIU ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Maturity Stage ◽  
Broccoli Sprouts ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Dpph Radical Scavenging

Sprouts are very rich in antioxidants (e.g. phenolic compounds) and their consumption is beneficial for humans’ health. The sprouts’ antioxidant properties are correlated to conditions of growth and maturity stage. The present study reveals the influence of maturity stage (5-, 7- and 9-day-old) and two sodium salts, NaCl and Na2SO4 (10 mM and 100 mM) on the antioxidant capacity and total phenolic content of cauliflower and broccoli sprouts. The analysed sprouts’ extracts were obtained in two phases, using ethanol 70% with 0.01% hydrochloric acid. In the case of cauliflower, the 7 days germination leads to the highest total phenolic content and radical scavenging capacity. Broccoli sprouts revealed the most enhanced DPPH radical scavenging capacity and total phenolic content at 5-day-old. The two sodium salts, generally, had positive effects on global antioxidant status. It is highlighted both for broccoli and cauliflower, the stimulating effect of 100 mM Na2SO4 on the total phenolic content and soluble dry weight, but the DPPH radical scavenging activity was more important in the case of NaCl, than Na2SO4 treatments, except for cauliflower sprouts treated with 100 mM Na2SO4. In conclusion, germination under adequate salt stress is a protocol to be considered in order to improve antioxidant properties of broccoli and cauliflower sprouts, despite the non-convenient effects on the germination and sprouts growth. Further researches are needed to optimize the level and time periods of such treatments.

scholarly journals Effect of Microwave Pretreatment of Seeds on the Quality and Antioxidant Capacity of Pomegranate Seed Oil

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1287
Author(s):  
Tafadzwa Kaseke ◽  
Umezuruike Linus Opara ◽  
Olaniyi Amos Fawole
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Oil Yield ◽  
Total Phenolic ◽  
Total Carotenoids ◽  
Yellowness Index ◽  
Microwave Pretreatment ◽  
Scavenging Capacity ◽  
Pomegranate Seeds

Microwave pretreatment of oilseeds is a novel technique used to enhance oil nutraceutical properties. In this study, the effect of microwave pretreatment of seeds was investigated on pomegranate seed oil quality attributes including oil yield, yellowness index, refractive index, peroxide value, ρ-anisidine value, total oxidation value, conjugated dienes, total phenolic content, total carotenoids content, phytosterol composition, fatty acid composition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, and ferric reducing antioxidant power (FRAP). The seeds of three different pomegranate cultivars (‘Acco’, ‘Herskawitz’, and ‘Wonderful’) were microwave heated at 261 W for 102 s. Pomegranate seeds microwave pretreatment enhanced oil yield, yellowness index, total carotenoids content, total phenolic content, FRAP and DPPH radical scavenging capacity, despite an increase in conjugated dienes, and peroxide value. Palmitic acid, oleic acid, linoleic acid, saturated, and monosaturated fatty acids were increased after pomegranate seeds microwave pretreatment, whilst the levels of punicic acid and β-sitosterol were reduced. Nevertheless, the refractive index, the ratio of unsaturated to saturated fatty acid of the extracted oil were not significantly (p > 0.05) affected by pomegranate seeds microwave pretreatment. Principal component analysis and agglomerative hierarchical clustering established that ‘Acco’ and ‘Wonderful’ oil extracts from microwave pretreated PS exhibited better oil yield, whilst ‘Herskawitz’ oil extracts showed higher total carotenoids content, total phenolic content, and antioxidant capacity.

scholarly journals Evaluation of Phenolic and Flavonoid Content, Antioxidant and Radical Scavenging Capacity of Baru (Dipteryx alata Vog.) Almond

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 326-326
Author(s):  
Deja Ivy ◽  
Rami Najjar ◽  
Maureen Meister ◽  
Maria Margareth Naves ◽  
Rafaela Feresin
Keyword(s):  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Freeze Dried ◽  
Trolox Equivalent ◽  
Phenolic And Flavonoid ◽  
Dipteryx Alata

Abstract Objectives The legume plant species Dipteryx alata Vog., commonly known as baru, is a tree that grows in the Brazilian savanna. It produces drupe type of fruit and has an edible seed referred to as the baru nut. The baru nut is of interest as it possesses bioactive compounds useful in managing chronic diseases, yet little is known about its composition. Thus, the aim of this study was to determine the phenolic and flavonoid content, as well as antioxidant and radical scavenging capacity of the baru nut. Methods Baru nuts were roasted, pulverized, and freeze-dried prior to polyphenolic extractions, which were performed using 80% ethanol and ultrasonic bath. Baru crude extracts were then purified with chloroform, evaporated, and freeze-dried prior to being assayed. Total phenolic content and total flavonoid content of purified baru polyphenolic extracts were determined using Folin-Ciocalteu reagent and aluminum chloride colorimetric assay, respectively. Antioxidant capacity was measured using Trolox equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant power (FRAP) while 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) assays were used to determine radical scavenging capacity. Results Total phenolic content of purified baru nut extract was 58 ± 10 μmol gallic acid equivalent/L while total flavonoid content was 7 ± 0 μg quercetin equivalent/mL. The TEAC of purified baru nut extract was 161 ± 5 μmol trolox equivalent (TE)/L, which was higher than its FRAP, 55 ± 0.5 μmol TE/L. Radical scavenging capacity of baru nut extract was 17 ± 3 and 5 ± 7 μmol TE/L according to DPPH and ORAC, respectively. Conclusions Baru nut is rich in polyphenols and has high antioxidant activity. Further research is warranted to determine the antioxidant properties of baru nut in vitro and in vivo. Funding Sources No sources of funding.

scholarly journals Characterization and Uptake of Strawberry-Derived Exosome-Like Nanovesicles by Human Aortic Endothelial Cells

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 310-310
Author(s):  
Jessica Danh ◽  
Brandon Canup ◽  
Rami Najjar ◽  
Maureen Meister ◽  
Hamed Laroui ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Aortic Endothelial Cells ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Human Aortic Endothelial Cells ◽  
Dpph Radical Scavenging

Abstract Objectives To characterize strawberry (SB)-derived exosome-like nanovesicles (ELNs), assess the total phenolic content, total flavonoid content, and total antioxidant capacity as well as its uptake by human aortic endothelial cells (HAECs). Methods SB ELNs were extracted using differential centrifugation. After final ultracentrifugation at 100,000 × g for 1 h, pellets were collected and washed in PBS. Characterization was performed using dynamic light scattering measurements. Total phenolic content (TPC) and flavonoid content (TFC) were determined using Folin-Ciocalteu and aluminum chloride, respectively. Antioxidant capacity was determined using the Trolox equivalent antioxidant capacity (TEAC) and ferric reducing ability of plasma (FRAP) assay while free radical scavenging power was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay. Cell viability and uptake were assessed in HAECs. Cell viability was measured after 24-h incubation with SB ELNs using MTT reagent. Cell uptake was measured after 12-h incubation with 100 μg/mL coumarin-6 (C-6) labelled SB ELNs. Fluorescent microscopy and flow cytometry were used to detect cellular uptake of C-6 labelled SB ELNs on a LSR II. Results SB ELNs were sized at 119.4 ± 28.3 nm (PDI = 0.29 ± 0.06). TPC and TFC of SB ELNs were 158.9 ± 22.6 μmol GAE/L and 5.1 ± 0.4 μg QE/mL, respectively. Antioxidant capacity of SB ELNs was 211.38 ± 6.3 μmol TE/L and 118.0 ± 7.6 μmol Fe2+/L by TEAC and FRAP, respectively. DPPH radical scavenging capacity was 181.3 ± 2.5 μmol TE/L in SB ELNs. No cytotoxic effects were observed for SB ELNs in HAECs. Uptake of SB ELNs by HAECs was 15.3% higher compared to baseline levels. Conclusions We report, for the first time, the presence of phenolics and flavonoids in the cargo of SB ELNs, SB ELNs antioxidant capacity, and demonstrate their uptake by HAECs. Taken together, these findings support the need to further characterize and explore the antioxidant potential of SB ELNs in vitro and in vivo. Funding Sources None.

scholarly journals Comparative analysis of specialized metabolites and antioxidant capacity in vitro of different natural populations of Globularia spp.

2018 ◽  
Vol 77 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Maja Friščić ◽  
Semir Maslo ◽  
Rade Garić ◽  
Željan Maleš ◽  
Kroata Hazler Pilepić
Keyword(s):  
Phenolic Content ◽  
Radical Scavenging ◽  
Natural Populations ◽  
Total Phenolic ◽  
Bioactive Substances ◽  
Specialized Metabolites ◽  
Plant Parts ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

AbstractTotal phenolic, flavonoid, condensed tannin and iridoid content, as well as antioxidant capacityin vitro,were determined spectrophotometrically in methanolic extracts of different plant parts of the Mediterranean medicinal plantGlobularia alypumL. and three widespread European species of the same genus:G. cordifoliaL.,G. meridionalis(Podp.) O. Schwarz andG. punctataLapeyr. In order to consider possible environmental influences on the production of specialized metabolites, each species, exceptG. alypum, was collected from three different natural populations. Great variations in the amounts of specialized metabolites were observed among different plant parts and species. For example, total phenolic content ranged from 10.13 (G. punctata, flowers) to 44.90 (G. cordifolia, flower stems) mg gallic acid equivalent g−1dry weight. Moreover, great differences, attributed to location-specific environmental factors, were observed among different populations of the same species. For example, a strong positive correlation was observed among mean monthly temperatures and total phenolic contents in the leaves of studiedGlobulariaspp. (r = 0.75, p = 0.019). However, despite these differences, all species were rich in bioactive substances when compared toG. alypum, especially in their aerial parts. A very good positive correlation was observed between total phenolic content and DPPH radical scavenging capacity (r = 0.86, p < 0.001)/ABTS radical scavenging capacity (r = 0.83, p < 0.001). The results obtained show thatG. cordifolia,G. meridionalisandG. punctataare rich in bioactive substances, providing support for their pharmaceutical utilization. Further investigations are needed to verify the possibility of their medicinal use.

Antioxidant capacity of hydroalcoholic extracts of Grewia serrulata DC and Grewia nervosa (Lour.) Panigrahi

2018 ◽  
Vol 9 (1) ◽  
pp. 121
Author(s):  
Mentham Ramesh ◽  
Chandu Babu Rao
Keyword(s):  
Total Phenolic Content ◽  
Superoxide Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential

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