Diverse Terpenoids and Their Associated Antifungal Properties from Roots of Different Cultivars of Chrysanthemum Morifolium Ramat

Roots provide anchorage and enable the absorption of water and micronutrients from the soil for plants. Besides these essential functions, roots are increasingly being recognized as an important organ for the production of diverse secondary metabolites. The goal of this study was to investigate the chemical composition and function of terpenoid secondary metabolites in roots of different cultivars of the popular ornamental plant Chrysanthemum morifolium Ramat. Although C. morifolium is known for rich production of secondary metabolites in its flower heads and leaves, the diversity of secondary metabolites in roots remains poorly characterized. In this study, 12 cultivars of C. morifolium were selected for comparative analysis. From their roots, a total of 20 terpenoids were detected, including four monoterpenes, 15 sesquiterpenes, and one diterpene. The cultivar ‘She Yang Hong Xin Ju’ exhibited the highest concentration of total terpenoids at approximately 730 µg·g−1 fresh weight. Most cultivars contained sesquiterpenes as the predominant terpenoids. Of them, (E)-β-farnesene was detected in all cultivars. Based on their terpenoid composition, the 12 cultivars were planed into four groups. To gain insights into the function of root secondary metabolites, we performed bioassays to assess their effects on growth of three species of pathogenic fungi: Fusarium oxysporum, Magnaporthe oryzae, and Verticillium dahliae. Significant variability in antifungal activity of the root extracts among different cultivars were observed. The cultivar ‘Xiao Huang Ju’ was the only cultivar that had significant inhibitory effects on all three species of fungi. Our study reveals the diversity of terpenoids in roots of C. morifolium and their function as a chemical defense against fungi.

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Characterization of Composition and Antifungal Properties of Leaf Secondary Metabolites from Thirteen Cultivars of Chrysanthemum morifolium Ramat

Molecules ◽

10.3390/molecules24234202 ◽

2019 ◽

Vol 24 (23) ◽

pp. 4202 ◽

Cited By ~ 2

Author(s):

Huanhuan Xue ◽

Yifan Jiang ◽

Hongwei Zhao ◽

Tobias G. Köllner ◽

Sumei Chen ◽

...

Keyword(s):

Secondary Metabolites ◽

Antifungal Agents ◽

Pathogenic Fungi ◽

Chrysanthemum Morifolium ◽

In Vitro Assays ◽

Gas Chromatography Mass Spectrometry ◽

Leaf Extracts ◽

Antifungal Properties ◽

Chrysanthemum Morifolium Ramat

Chrysanthemum morifolium Ramat is an ornamental plant of worldwide cultivation. Like many other species in the family Asteraceae, C. morifolium is a rich producer of secondary metabolites. There are two objectives in this study: (I) to determine and compare the diversity of apolar secondary metabolites among different cultivars of C. morifolium and (II) to compare their properties as antifungal agents. To attain these objectives, we selected 13 cultivars of C. morifolium that are commonly used for making chrysanthemum tea as experimental materials. Leaves at the same developmental stage were collected from respective mature plants and subjected to organic extraction. The extracts were analyzed using gas chromatography–mass spectrometry. A total of 37 apolar secondary metabolites including 26 terpenoids were detected from the 13 cultivars. These 13 cultivars can be largely divided into three chemotypes based on chemical principal components analysis. Next, the extracts from the 13 cultivars were examined in in vitro assays for their antifungal properties against three species of pathogenic fungi: Fusarium oxysporum, Magnaporthe oryzae, and Verticillium dahliae. Significant variability in antifungal activity of the leaf extracts among different cultivars was observed. The 13 cultivars can be divided into four groups based on their antifungal activities, which could be partly correlated to the contents of terpenoids. In short, this study reveals large variations in chemical composition, particularly of terpenoids, of leaf secondary metabolites among different cultivars of C. morifolium and their different abilities in functioning as antifungal agents.

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Inhibitory Effects of Chrysanthemum (Chrysanthemum morifolium Ramat.) Extract and Its Active Compound Isochlorogenic Acid A on Sarcopenia

Preventive Nutrition and Food Science ◽

10.3746/pnf.2021.26.4.408 ◽

2021 ◽

Vol 26 (4) ◽

pp. 408-416

Author(s):

Dowan Kwon ◽

Changhee Kim ◽

Yu Kyong Woo ◽

Jae-Kwan Hwang

Keyword(s):

Active Compound ◽

Chrysanthemum Morifolium ◽

Inhibitory Effects ◽

Chrysanthemum Morifolium Ramat

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MORFOLOGI DAN ANATOMI Chrysanthemum morifolium Ramat. var. puspita nusantara dan var. tirta ayunisertaChrysanthemum indicum L.var. mustika kaniya

Al-Hayat: Journal of Biology and Applied Biology ◽

10.21580/ah.v2i2.4660 ◽

2019 ◽

Vol 2 (2) ◽

pp. 53

Author(s):

Isvana Dalaila ◽

Kusrinah Kusrinah ◽

Lianah Lianah

Keyword(s):

Economic Value ◽

Chrysanthemum Morifolium ◽

Ornamental Plant ◽

Anatomical Characteristics ◽

Anatomical Characters ◽

Chrysanthemum Morifolium Ramat

Chrysanthemum including ornamental plant commodities are much-loved community. Chrysanthemum has a high economic value and potential to be developed. The diversity of morphological and anatomical characters is not currently widely practiced. This study aims to determine the morphological and anatomical characteristics of C. morifolium Ramat. var. puspitanusantara, and var. tirtaayuni and C. indicum L. var. mustikakaniya. Sampling by purposive random. The study was conducted in May - June 2017 in Kenteng Village, Bandungan District, Semarang District. The results showed that the results showed that C. morifolium Ramat var. puspitanusantara and var. tirtaayuni and C. indicum L. var. mustikakaniya have variations of morphology, especially on leaves and flowers, variations in leaf organ form, and base. Variations of flowering organs include the number of flowers per stem, the number of ribbon flowers, and the freshness of the flower. Anatomical characters based on tissue arrangement of stem and leaf organs have similarities.

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Analysis of Secondary Metabolites of Shoot, Callus Culture and Field Plant of Chrysanthemum morifolium Ramat

Jurnal ILMU DASAR ◽

10.19184/jid.v21i1.8665 ◽

2020 ◽

Vol 21 (1) ◽

pp. 1

Author(s):

Tia Setiawati ◽

Alma Ayalla ◽

Mohamad Nurzaman ◽

Valentina A. Kusumaningtyas ◽

Ichsan Bari

Keyword(s):

Secondary Metabolites ◽

Secondary Metabolite ◽

Callus Culture ◽

Leaf Explants ◽

Shoot Culture ◽

Chrysanthemum Morifolium ◽

Gas Chromatography Mass Spectrometry ◽

Stem Explants ◽

Chrysanthemum Plant ◽

Chrysanthemum Morifolium Ramat

The chrysanthemum plant (Chrysanthemum morifolium Ramat.) contains many secondary metabolites such as flavonoids and various volatile compounds that can be utilized as drugs. Tissue culture can be an alternative to enhance the production of certain secondary metabolite. The study aimed to determine the types of secondary metabolites that contained in shoot culture, callus and field plants of C. morifolium. The research method was exploration in the laboratory to analyze and compare the content of secondary metabolite from shoot culture, callus and field plants of C. morifolium. Callus was induced by explants of C. morifolium plantlet stems and leaves respectively on MS medium with an addition of 3 ppm 2,4-D + 2 ppm kinetin and 4 ppm 2,4-D. For shoot culture, single nodule explants with one leaf were planted on MS media with the addition of 1 ppm BAP. The secondary metabolite compouds were analized and identified by GC-MS (Gas Chromatography-Mass Spectrometry). The results showed that various types of secondary metabolites contained in shoot culture, callus and field plants of C. morifolium. In callus culture from leaf explants, four compounds from groups of alcohol, acetic acid and organosilicon were identified, whereas in callus culture from stem explants were identified eight compounds from aldehydes, esters, alkanes, and carboxylic acids group. In the shoot culture, nine compounds of alcohol, ketone, aldehyde, cycloalkane and organosilicon group were identified, while in the field plants five compounds were identified from the cycloalkanes, ketones, organoborones and organosilicon group. Some detected compounds have a potential as precursors of alkaloid, phenolic, and flavonoid.Keywords: chrysanthemum, culture, shoots, callus, secondary metabolites.

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Effects of Planting Density and Concentration of NPK Fertilizer on the Growth of Potted Chrysanthemum (Chrysanthemum morifolium Ramat)

JURNAL AGRONIDA ◽

10.30997/jag.v4i1.1530 ◽

2019 ◽

Vol 4 (1) ◽

Author(s):

Feby Steviani Anugrah Ramadhan ◽

Setyono Setyono ◽

Evi Dwi Sulistya Nugroho

Keyword(s):

Economic Value ◽

Chrysanthemum Morifolium ◽

Ornamental Plant ◽

Stem Diameter ◽

Planting Density ◽

Randomized Design ◽

Ornamental Crops ◽

Npk Fertilizer ◽

Completely Randomized Design ◽

Chrysanthemum Morifolium Ramat

Chrysanthemum is an ornamental plant that people likes, grows the whole year, and hashigh economic value. This study was aimed at assessing the effects of planting density andconcentration ofNPK fertilizer on the growth of potted chrysanthemum. The study was conductedin a plastic house at the Indonesian Ornamental Crops Research Institute (IOCRI), Cipanas,Cianjur, West Java from March to June 2017. Shoot cuttings of ±7 cm of potted chrysanthemum ofAvanthe Agrihorti cultivar were used. A completely randomized design in a factorial pattern withfactors was used. The first factor was planting density and the second factor was concentrationofNPK (16:16:16) fertilizer. The planting density consisted of 5, 6, and 7 shoot cuttings andconcentrationof NPK fertilizer were 150, 200, 250, and 300 ppm. Results showed that plantingdensity significantly affected stem diameter, number of buds, and width of canopy. No effectconcentrationof fertilizer was found on all variables.Keywords: potted chrysanthemum, planting density, NPK fertilizer

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Effect of benzyl amino purine and 1-methylcyclopropene in maintaining rooting quality of chrysanthemum (Chrysanthemum morifolium Ramat cv. ‘White Fiji’) cuttings

Research on Crops ◽

10.31830/2348-7542.2020.024 ◽

2020 ◽

Vol 21 (1) ◽

Keyword(s):

Chrysanthemum Morifolium ◽

Chrysanthemum Morifolium Ramat ◽

Benzyl Amino Purine

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Effect of triacontanol on production and quality of flowers of Chrysanthemum morifolium Ramat

Scientia Horticulturae ◽

10.1016/0304-4238(82)90107-8 ◽

1982 ◽

Vol 18 (1) ◽

pp. 87-92 ◽

Cited By ~ 5

Author(s):

D. Skogen ◽

A.B. Eriksen ◽

S. Nilsen

Keyword(s):

Chrysanthemum Morifolium ◽

Chrysanthemum Morifolium Ramat

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A Cross-Metabolomic Approach Shows that Wheat Interferes with Fluorescent Pseudomonas Physiology through Its Root Metabolites

Metabolites ◽

10.3390/metabo11020084 ◽

2021 ◽

Vol 11 (2) ◽

pp. 84

Author(s):

Laura Rieusset ◽

Marjolaine Rey ◽

Florence Gerin ◽

Florence Wisniewski-Dyé ◽

Claire Prigent-Combaret ◽

...

Keyword(s):

Secondary Metabolites ◽

Host Plant ◽

Soil Microorganisms ◽

Wheat Root ◽

Bioactive Metabolites ◽

Root Extracts ◽

Fluorescent Pseudomonas ◽

Health And Development ◽

The Impact ◽

Metabolomic Approach

Roots contain a wide variety of secondary metabolites. Some of them are exudated in the rhizosphere, where they are able to attract and/or control a large diversity of microbial species. In return, the rhizomicrobiota can promote plant health and development. Some rhizobacteria belonging to the Pseudomonas genus are known to produce a wide diversity of secondary metabolites that can exert a biological activity on the host plant and on other soil microorganisms. Nevertheless, the impact of the host plant on the production of bioactive metabolites by Pseudomonas is still poorly understood. To characterize the impact of plants on the secondary metabolism of Pseudomonas, a cross-metabolomic approach has been developed. Five different fluorescent Pseudomonas strains were thus cultivated in the presence of a low concentration of wheat root extracts recovered from three wheat genotypes. Analysis of our metabolomic workflow revealed that the production of several Pseudomonas secondary metabolites was significantly modulated when bacteria were cultivated with root extracts, including metabolites involved in plant-beneficial properties.

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